when do isotypes matter? the primary -> secondary immune response: increase ig titer switch from...
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When Do Isotypes When Do Isotypes Matter?Matter?
The primary -> secondary immune response:
•Increase Ig titer
•Switch from IgM to IgG
•Increase Ag binding affinity
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Where and when do affinity Where and when do affinity maturations and isotype class switch maturations and isotype class switch
occur??occur??
BCR:mIgMBCR:mIgM sIgGsIgGmIgGmIgG
CSR/SHM
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Infection and Ag presentationInfection and Ag presentation
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Infection and Ag presentationInfection and Ag presentation
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Infection and Ag presentationInfection and Ag presentation
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T cell zone
B cell zone
Red pulp border
Generation of Generation of plasmablastsplasmablastsin the spleenin the spleen
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T cells: CD4+ and CD8+T cells: CD4+ and CD8+
CD8+
CD4+
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APCs: present Ag in the context APCs: present Ag in the context of MHC class II to T helper cellsof MHC class II to T helper cells
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Ag presentation to T helper cells Ag presentation to T helper cells in the spleenin the spleen
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T helper cells are CD4+T helper cells are CD4+
•are activated by APCare activated by APC
•secrete cytokines tosecrete cytokines toaugment the immuneaugment the immuneresponse response
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T helper cells polarizeT helper cells polarize into Tinto THH1 and T1 and THH2 cells2 cells
and secrete uniqueand secrete unique combinations of combinations of cytokinescytokines
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Cytokines target germline transcripts Cytokines target germline transcripts to specific Ig isotypes in B cellsto specific Ig isotypes in B cells
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Cytokines target germline transcripts Cytokines target germline transcripts to specific isotypes to specific isotypes
33 2b2b
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T cell zoneB cell zone
Red pulp border
Germinal Center
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SHM Occurs in SHM Occurs in the Germinal the Germinal
Center B CellsCenter B Cells
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Primary focus
SHM Occurs in Germinal Center B CellsSHM Occurs in Germinal Center B Cells
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T cell zone
Red pulp border
Germinal Center
Primary focus
plasmablasts
T cell zone
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sIgM sIgM and sIgG
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