what is the qtls and n0n- allelic heterosis...the goal of rice breeding program in korea (iii)...

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What is the QTLs and N0N- Allelic Heterosis ? QTLs?, Non-allelic Heterosis ?

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Page 1: What is the QTLs and N0N- Allelic Heterosis...The Goal of Rice Breeding Program in KOREA (III) Development of value added Functional rice cultivar ¾ Low-albumen rice (low-Glutelin

What is the QTLs and N0N- Allelic Heterosis ?

QTLs?,

Non-allelic Heterosis ?

Page 2: What is the QTLs and N0N- Allelic Heterosis...The Goal of Rice Breeding Program in KOREA (III) Development of value added Functional rice cultivar ¾ Low-albumen rice (low-Glutelin

Wide Hybridization

• To transfer resistance to diseases and insects intojaponica rice : Bl, BB, BPH, RBSDV, ShB, Stemborer

• Transfer of genes for weed competitive ability from African rice (O. glaberrima) into japonica rice

• Molecular characterization of alien introgression- Tagging of alien genes/QTLs and molecular cytogenetics

• Development of near-isogenic alien introgression lines for use in functional genomics

Page 3: What is the QTLs and N0N- Allelic Heterosis...The Goal of Rice Breeding Program in KOREA (III) Development of value added Functional rice cultivar ¾ Low-albumen rice (low-Glutelin

Production of F1 hybrids between japonica cultivars and wild species of Oryza (’03 – ’06)

Completely sterileEmbryo rescueO. minuta (BBCC)xIlpumbyeo

Completely sterile, limited pairing, irregular meiosis

Embryo rescueO. minuta (BBCC)xJunambyeo

Completely sterileEmbryo rescueO. alta (CCDD)xJunambyeo

Completely sterileDirect crossO. punctata (BB)xIlpumbyeo

Highly sterileDirect crossO. glaberrima (AA)xHwacheongbyeo

Highly sterileDirect crossO. longistaminata AA)xIlpumbyeo

Partially sterileDirect crossO. rufipogon (AA)xIlpumbyeo

Seedlings in test tubesEmbryo rescueO. ridleyi (HHJJ)xIlpumbyeo

Completely sterileEmbryo rescueO. officinalis (CC)xJinmibyeo

Intermediate in morphology, partially sterile, normal chromosome pairing

Direct crossO. rufipogon (AA)xJinmibyeo

Wild speciesO. sativa

RemarksMethodF1 hybrids

Page 4: What is the QTLs and N0N- Allelic Heterosis...The Goal of Rice Breeding Program in KOREA (III) Development of value added Functional rice cultivar ¾ Low-albumen rice (low-Glutelin

Breeding of elite lines from a cross ofHwaseonbyeo x O. minuta (2n=48, BBCC)

* Bl, Blast; BB, Bacterial blight; BSDV, Black streak dwarf virus

8.14

8. 21

8.13

8.12

78

72

109

85

VarietyHeading (mon.day)

Culmlength(cm)

Yield(t/ha)

Suweon 487

Suweon 497

Suweon 506

Hwaseongbyeo

Disease resistance Bl BB BSDV

(1-9) (1-9) (%)

7

7

1

7

7

1

1

8

0

ND

ND

56.3

5.46

4.94

6.14

5.22

‘02

‘03

‘04

-

Year

Page 5: What is the QTLs and N0N- Allelic Heterosis...The Goal of Rice Breeding Program in KOREA (III) Development of value added Functional rice cultivar ¾ Low-albumen rice (low-Glutelin

Blast

Bph

Evaluation of Wide Cross Progenies

BLB

RBSDV

Page 6: What is the QTLs and N0N- Allelic Heterosis...The Goal of Rice Breeding Program in KOREA (III) Development of value added Functional rice cultivar ¾ Low-albumen rice (low-Glutelin

Introgression Lines from Crosses of Japonica x Wild Rices

Page 7: What is the QTLs and N0N- Allelic Heterosis...The Goal of Rice Breeding Program in KOREA (III) Development of value added Functional rice cultivar ¾ Low-albumen rice (low-Glutelin

Breeding ProgramIn KOREA

Page 8: What is the QTLs and N0N- Allelic Heterosis...The Goal of Rice Breeding Program in KOREA (III) Development of value added Functional rice cultivar ¾ Low-albumen rice (low-Glutelin

Targets of Rice Breeding

For Farmers

For Consumers

For Environment

For Future

Higher income(yield/quality)Easier cultivationResistance to biotic and abiotic stressLess materials

Good taste to be safeNutrient to be cheapEasy to processRich in functional ingredients

Less chemicalsEnrich biodiversityBeautiful landscape

Super high yieldingSustain high yielding systemEnhance protein and oil yield

Page 9: What is the QTLs and N0N- Allelic Heterosis...The Goal of Rice Breeding Program in KOREA (III) Development of value added Functional rice cultivar ¾ Low-albumen rice (low-Glutelin

Rice Breeding Program in KOREA

High quality

Super Yielding,Hybrid

Low Input,Direct Seeding,Mechanization

Safety

Short-term,Cropping System

Bio-fortifications

Page 10: What is the QTLs and N0N- Allelic Heterosis...The Goal of Rice Breeding Program in KOREA (III) Development of value added Functional rice cultivar ¾ Low-albumen rice (low-Glutelin

The Goal of Rice Breeding Program in KOREA (I)

Development of high-palatable rice cultivars each institute

Central zone adaptation with blast, brown planthopper

resistance and with cold tolerance

West-southern region adaptation with bacterial blight

resistance and with salinity tolerance

East-southern region adaptation with virus resistance and

with cold salty wind tolerance

Page 11: What is the QTLs and N0N- Allelic Heterosis...The Goal of Rice Breeding Program in KOREA (III) Development of value added Functional rice cultivar ¾ Low-albumen rice (low-Glutelin

The Goal of Rice Breeding Program in KOREA (II)

Development of high-quality rice cultivars adaptable to direct seeding

Lodging resistanceBetter germination & emergence at low temperatureHigh-adaptability in dense planting

Development of short-term rice cultivars adaptable tolate transplanting after cash crops

Growth duration : shorter than 95daysGrain productivity per day : above 60 kg/ha

Page 12: What is the QTLs and N0N- Allelic Heterosis...The Goal of Rice Breeding Program in KOREA (III) Development of value added Functional rice cultivar ¾ Low-albumen rice (low-Glutelin

The Goal of Rice Breeding Program in KOREA (III)

Development of value added Functional rice cultivarLow-albumen rice (low-Glutelin rice) for treatment and prevention of diabetes, kidney disease, chronic renal failure, etc.

Developing rice varieties that have 16kDa globulin removed and have

anti-allergy property (decreases atopy, etc.)

Developing iron-fortified rice varieties for pregnant women to prevent eficiency of red blood cells or anemia

Developing varieties with antioxidant function, such as high Anthocyan content in black brown rice.

Developing gamma aminohutyric acid in rice for blood pressure letdown

Developing Pro-vitamin A in rice for night blindness prevention

Developing Isoflavone in rice varieties for the control of female hormone

Developing low-content protein in rice for wine

Page 13: What is the QTLs and N0N- Allelic Heterosis...The Goal of Rice Breeding Program in KOREA (III) Development of value added Functional rice cultivar ¾ Low-albumen rice (low-Glutelin

The Goal of Rice Breeding Program in KOREA (IV )

Development of new rice materials using biotechnologyEnhancement of transgenic or regeneration efficiencyIdentification & isolation of useful gene sources

Development of super-yielding rice cultivarsTarget (2010) : 10 t/ha in milled rice

Multi-resistance to major diseases and insect pestsWhole crop silage 20 t/ha in korea

New rice materials for super-yielding hybrid riceUtilization of wide-cross compatibility genesEnhancement of hybrid-seed productivity

Page 14: What is the QTLs and N0N- Allelic Heterosis...The Goal of Rice Breeding Program in KOREA (III) Development of value added Functional rice cultivar ¾ Low-albumen rice (low-Glutelin

The Vision of Rice Breeding in KOREA

2015

Milled rice yield(t/ha)

hHigh-quality rice : 6.5hHigh-yielding rice : 10.0

Safety in cultivation

h Stability of resistance to disease & insect pests

h Tolerance/Resistance toenvironmental stressessuitable for regional specificity

Grain quality

High quality rice- Marketing & milling quality - Eating qualityHigh-yielding rice

- Diversified grain size, shape- Physicochemical propertiesValue added functional .Double cropping & Reduction cost

hShort-growth duration- Less than 100 days.- Grain yield : > 6.0kg/day/10a

hAdaptability to direct seeding- Lwo-temp germinability - Lodging tolerance, - Low tillering : < 10 tillers

Page 15: What is the QTLs and N0N- Allelic Heterosis...The Goal of Rice Breeding Program in KOREA (III) Development of value added Functional rice cultivar ¾ Low-albumen rice (low-Glutelin

Rice Breeding Practices