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A TraditionalA TraditionalGenBank RecordGenBank Record

LOCUS AY182241 1931 bp mRNA linear PLN 04-MAY-2004DEFINITION Malus x domestica (E,E)-alpha-farnesene synthase (AFS1) mRNA,

complete cds.ACCESSION AY182241VERSION AY182241.2 GI:32265057KEYWORDS .SOURCE Malus x domestica (cultivated apple)

ORGANISM Malus x domesticaEukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;Spermatophyta; Magnoliophyta; eudicotyledons; core eudicots;rosids; eurosids I; Rosales; Rosaceae; Maloideae; Malus.

REFERENCE 1 (bases 1 to 1931)AUTHORS Pechous,S.W. and Whitaker,B.D.TITLE Cloning and functional expression of an (E,E)-alpha-farnesene

synthase cDNA from peel tissue of apple fruitJOURNAL Planta 219, 84-94 (2004)

REFERENCE 2 (bases 1 to 1931)AUTHORS Pechous,S.W. and Whitaker,B.D.TITLE Direct SubmissionJOURNAL Submitted (18-NOV-2002) PSI-Produce Quality and Safety Lab,

USDA-ARS, 10300 Baltimore Ave. Bldg. 002, Rm. 205, Beltsville, MD20705, USA

REFERENCE 3 (bases 1 to 1931)AUTHORS Pechous,S.W. and Whitaker,B.D.TITLE Direct SubmissionJOURNAL Submitted (25-JUN-2003) PSI-Produce Quality and Safety Lab,

USDA-ARS, 10300 Baltimore Ave. Bldg. 002, Rm. 205, Beltsville, MD20705, USA

REMARK Sequence update by submitterCOMMENT On Jun 26, 2003 this sequence version replaced gi:27804758.FEATURES Location/Qualifiers

source 1..1931/organism="Malus x domestica"/mol_type="mRNA"/cultivar="'Law Rome'"/db_xref="taxon:3750"/tissue_type="peel"

gene 1..1931/gene="AFS1"

CDS 54..1784/gene="AFS1"/note="terpene synthase"/codon_start=1/product="(E,E)-alpha-farnesene synthase"/protein_id="AAO22848.2"/db_xref="GI:32265058"/translation="MEFRVHLQADNEQKIFQNQMKPEPEASYLINQRRSANYKPNIWKNDFLDQSLISKYDGDEYRKLSEKLIEEVKIYISAETMDLVAKLELIDSVRKLGLANLFEKEIKEALDSIAAIESDNLGTRDDLYGTALHFKILRQHGYKVSQDIFGRFMDEKGTLEDFLHKNEDLLYNISLIVRLNNDLGTSAAEQERGDSPSSIVCYMREVNASEETARKNIKGMIDNAWKKVNGKCFTTNQVPFLSSFMNNATNMARVAHSLYKDGDGFGDQEKGPRTHILSLLFQPLVN"

ORIGIN 1 ttcttgtatc ccaaacatct cgagcttctt gtacaccaaa ttaggtattc actatggaat

61 tcagagttca cttgcaagct gataatgagc agaaaatttt tcaaaaccag atgaaacccg121 aacctgaagc ctcttacttg attaatcaaa gacggtctgc aaattacaag ccaaatattt181 ggaagaacga tttcctagat caatctctta tcagcaaata cgatggagat gagtatcgga241 agctgtctga gaagttaata gaagaagtta agatttatat atctgctgaa acaatggatt

//

Header

Feature Table

Sequence

The Flatfile FormatThe Flatfile Format

Traditional GenBank RecordTraditional GenBank Record

ACCESSION U07418

VERSION U07418.1 GI:466461

ACCESSION U07418

VERSION U07418.1 GI:466461

Accession•Stable•Reportable•Universal

Accession•Stable•Reportable•Universal

VersionTracks changes in sequenceVersionTracks changes in sequence

GI numberNCBI internal useGI numberNCBI internal use

well annotatedwell annotated

the sequence is the datathe sequence is the data

LOCUS AF062069 3808 bp mRNA INV 02-MAR-2000

Sequence and Database IdentifiersSequence and Database IdentifiersLocus, accession, Locus, accession, gigi, version, version

DEFINITION Limulus polyphemus myosin III mRNA, complete cds.

GB DivisionLocus Name

DEF line (Title)

Modification Datemol-typemRNA (= cDNA)rRNAsnRNADNA

Sequencelength

VERSION AF062069.2 GI:7144484

ACCESSION AF062069 Accession Number

Accession.version gi number

BASE COUNT 1201 a 689 c 782 g 1136 tORIGIN

1 tcgacatctg tggtcgcttt ttttagtaat aaaaaattgt attatgacgt cctatctgtt<sequence omitted>

3721 accaatgtta taatatgaaa tgaaataaag cagtcatggt agcagtggct gtttgaaata3781 aagatacagt aactagggaa aaaaaaaa

//

SequenceSequence

End of record

Indicates beginning of sequence data

Using Using EntrezEntrez

An integrated An integrated database search and database search and

retrieval systemretrieval system

WWWWWWAccessAccess

Entrez&BLAST

GenomesGenomes

TaxonomyTaxonomy

EntrezEntrez: Neighboring and Hard Links: Neighboring and Hard Links

PubMedabstractsPubMedabstracts

Nucleotide sequences

Nucleotide sequences

Protein sequencesProtein

sequences

3-D Structure(MMDB)(MMDB)

3 -D Structure

3 -D Structure

Word weight

PhylogenyPhylogenyVAST

BLASTBLAST

WWW WWW EntrezEntrez•All of MEDLINE plus others•Abstracts•Links to online Journals

GenBank, EMBL, DDBJRefSeq, PDB

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MIMNow in Entrez

Population and phylogenetic studies

EntrezEntrez NucleotidesNucleotides

Mouse

Document Summaries: Mouse[All Fields]Document Summaries: Mouse[All Fields]

Chicken not mouse !?

3 million records

EntrezEntrez Nucleotides: Limits: Preview/IndexNucleotides: Limits: Preview/Index

Mouse

EntrezEntrez Nucleotides: LimitsNucleotides: LimitsAccessionAll FieldsAuthor NameEC/RN NumberFeature keyFilterGene NameIssueJournal NameKeywordModification DateOrganismPage NumberPrimary AccessionPropertiesProtein NamePublication DateSeqID StringSequence LengthSubstance NameText WordTitle WordUidVolume

Field Restriction

Only FromRefSeqGenBankEMBLDDBJ

Exclude unwanted categories of sequences

MoleculeGenomic DNA/RNAmRNArRNA

Gene LocationGenomic DNA/RNAMitochondrionChloroplast

Mouse

EntrezEntrez Nucleotides: Limits: OrganismNucleotides: Limits: Organism

Mouse

Document Summaries: Mouse[Organism]Document Summaries: Mouse[Organism]

2,976,070[All Fields]-2,921,009[Organism]

55,061

Exclude Bulk Sequences, mRNAExclude Bulk Sequences, mRNA

Adding Terms: Preview/IndexAdding Terms: Preview/Index

glyceraldehyde 3 phosphate dehydrogenase

AccessionAll FieldsAuthor NameEC/RN NumberFeature keyFilterGene NameIssueJournal NameKeywordModification DateOrganismPage NumberPrimary AccessionPropertiesProtein NamePublication DateSeqID StringSequence LengthSubstance NameText WordTitle WordUidVolume

Search History

Mouse GAPD RecordsMouse GAPD Records

(("Mus musculus"[Organism] ANDglyceraldehyde 3 phosphate dehydrogenase[Title Word])AND ((((((1900[MDAT] : 3000[MDAT]) NOT gbdiv_est[PROP]) NOT gbdiv_sts[PROP]) NOT gbdiv_gss[PROP])NOT gbdiv_htg[PROP]) NOT gbdiv_pat[PROP]))AND biomol_mrna[PROP]

Properties Field Terms

Displaying Mouse GAPD RecordsDisplaying Mouse GAPD Records

SummaryBriefGenBankASN.1FASTAGI listLinkOutPubMed LinksProtein LinksNucleotide NeighborsPopSet LinksStructure LinksGenome LinksTaxonomy LinksOMIM Links

Formats

Links and neighbors (related records)

>gi|193425|gb|M60978.1|MUSGAPDS Mus musculus testis-specific isoform of glyceraldGGCAGCCAGGCCATGAGATCTTAGGCCATGTCGAGACGTGACGTGGTCCTTACCAATGTTACTGTTGTCCAGCTACGGCGGGACCGATGCCCATGCCCATGCCCATGCCCATGTCCATGCCCATGCCCTGTGATCAGACCACCTCCACCCAAGCTTGAGGATCCACCACCCACGGTTGAAGAACAGCCACCGCCACCGCCGCCGCCACCTCCACCTCCACCACCACCTCCTCCTCCTCCTCCACCCCAGATAGAGCCAGACAAGTTTGAAGAGGCTCCCCCTCCCCCTCCCCCTCCTCCTCCTCCTCCCCCTCCCCCTCCTCCACCACTCCAAAAGCCAGCTAGAGAGCTGACAGTGGGTATCAATGGATTTGGACGCATTGGTCGTCTGGTGCTGCGAGTCTGCATGGAGAAGGGCATTAGGGTGGTAGCAGTGAATGACCCATTCATTGATCCAGAATACATGGTTTACATGTTCAAATATGACTCCACACATGGTAGATACAAAGGAAACGTGGAACATAAGAATGGACAACTAGTTGTGGACAACCTTGAGATCAACACGTACCAGTGCAAAGACCCTAAAGAAATCCCCTGGAGCTCTATAGGGAATCCCTACGTGGTGGAGTGTACAGGCGTCTATCTGTCCATCGAGGCAGCTTCGGCACATATTTCATCTGGTGCCAGGCGTGTGGTGGTCACTGCACCCTCCCCCGATGCACCCATGTTTGTCATGGGAGTGAACGAGAAGGACTATAACCCTGGCTCTATGACCATTGTCAGCAATGCATCCTGTACCACCAACTGCCTGGCTCCTCTCGCCAAGGTTATTCATGAAAACTTCGGGATCGTGGAAGGGCTAATGACCACAGTCCATTCCTACACAGCCACTCAGAAGACAGTGGATGGGCCATCAAAGAAGGACTGGCGAGGTGGCCGCGGCGCTCACCAAAACATCATCCCATCGTCCACTGGGGCTGCCAAGGCTGTAGGCAAAGTCATCCCAGAGCTCAAAGGGAAGCTAACAGGAATGGCATTCCGGGTGCCAACCCCAAACGTGTCAGTTGTGGACCTGACCTGCCGCCTGGCCAAGCCTGCTTCTTACTCGGCTATCACGGAGGCTGTGAAAGCTGCAGCCAAGGGACCTTTGGCTGGCATCCTTGCTTACACAGAGGACCAGGTGGTCTCCACGGACTTTAACGGCAATCCCCATTCTTCCATCTTTGATGCTAAGGCTGGAATTGCCCTCAATGACAACTTCGTGAAGCTTGTTGCCTGGTACGACAACGAATATGGCTACAGTAACCGAGTGGTCGACCTCCTCCGCTACATGTTTAGCCGAGAGAAGTAACACAAAAGGCCCCTCCTTGCTCCCCTGCGCACCTCGCGTTCCTGACTTCGGCTTCCACTCAAAGGCGCCGCCACCGGGTCAACAATGAAATAAAAACGAGAATGCGC

>

FASTA FormatFASTA Format

FASTA Definition Line>gi|193425|gb|M60978.1|MUSGAPDS

gi number

Database Identifiersgb GenBankemb EMBLdbj DDBJsp SWISS-PROTpdb Protein Databankpir PIRprf PRFref RefSeq

Accession number

Locus Name

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