week 5: electronic cell counters instrumentation automation electric impedance coulter principle...

Download Week 5: Electronic Cell Counters Instrumentation Automation Electric impedance Coulter principle Optical scatter Myeloperoxidase Radio frequency probe

Post on 28-Dec-2015

218 views

Category:

Documents

0 download

Embed Size (px)

TRANSCRIPT

  • Week 5: Electronic Cell CountersInstrumentationAutomationElectric impedanceCoulter principle

    Optical scatterMyeloperoxidaseRadio frequency probeHistogramData plot

  • Instrumentation/AutomationIncrease productivity and precisionAccuracy still depends on operatorOther interventionsCalibrationQCMaintenance

  • Brief History1852: Hemocytometry by K Vierordt1956: Electronic impedance counter Coulter Model A1970s: Light scatter technique (e.g., Ortho ELT-81980s: Cytochemical counter Technicon H-6000; flowcytometry1990s: VCS technology of Coulter STKS

  • Electrical ImpedanceCoulter principle first developed in 1950s

    R = k x Particle volume Aperture size

  • Coulters A, F, ZBI

  • Coulters S and S-Plus

  • Light ScatterDegree of light scatter is proportional to cell sizeUse of laminar flow using sheath fluid prevents cells from tumblingMore precise cell grouping with size: differential count

  • Ortho ELT-8

  • CytochemicalTechnicon measured the myeloperoxidase activity of leukocytes along with light scatter to differentiate leukocytes more preciselyDevelopment of flowcytometry: cell marker studies, DNA analysis, etc.

  • Light Scatter and Myeloperoxidase Activity

  • Radio Frequency ProbeVCS (volume, conductivity, scatter) technology by CoulterRadio frequency probe with impedance by SysmexAble to determine cell surface features and internal (nuclear, granular) complexity

  • Sizing and Conductivity