SCIENTIFIC PROGRAMME WEDNESDAY 16TH

12.30

Opening lecture

Vivek Malhotra (Centre for Genomic Regulation, Barcelona):

Mechanism of intracellular protein sorting ROOM A

15.45

Session I: Advances in new technologies ROOM A

Session chairs: Alberto Alvarez-Barrientos (Projech Science to Technology,

Madrid) and María Carmen Risueño (CIB, CSIC Madrid).

Invited speakers

David Klenerman (Univ of Cambridge, UK):

Studying single molecules on living cells

15.45

Philip N. Benfey (Duke University, Durham, NC, USA):

Systems Biology and Development 16.30

Isabel Díaz (Centro de Biotecnología y Genómica de plantas, Univ.

Politécnica de Madrid):

Bimolecular complementation assays to study protein-protein interactions in plants

17.15

Carmen López Iglesias (Scientifical and Technical Services,

University of Barcelona) :

Approaching the native state: cryo-technologies in electron microscopy

18.30

Sergio Marco (Institut Curie, Paris):

Progress in transmission electron tomography: high resolution 3D structure of cell organelles

19.00

THURSDAY 17TH 8.00

Session II: Proliferation, differentiation and cell death ROOM A

Session chairs: Alberto Muñoz (Centre for Biomedical Research “Alberto Sols”)

and Guillermo López (Pablo de Olavide University).

Invited speakers

María Cuervo (Albert Einstein College of Medicine, New York):

Autophagy in aging and age-related disorders

8.00

Pura Muñoz Cánoves (Pompeu Fabra University):

Mechanisms regulating muscle cell differentiation, growth and regeneration

8.30

Oral communications

1. Poly(ADP-ribose)polymerase-1 expression and activity increase coincidentally with cell death in early postnatal mouse retina. David Martín-Oliva, Rosa M. Ferrer, Ana M.

Santos, M. Carmen Carrasco-Sierra, Ana Sierra, José Luis

Marin-Teva, Ruth Calvente, Julio Navascués, Miguel A.

Cuadros.

9.00

2. Functional activity of Sur8 depends of its specific plasma membrane microdomain localization. José Luis Oliva,

Gonzalo León, Natalia Martínez, Lucía Sanchez-Ruiloba,

Teresa Iglesias, Piero Crespo and José María Rojas.

9.15

3. Catecholamines induce cardiac fibroblast autophagy

through activation of the β2-adrenergic receptor. Pablo

9.30

Aránguiz-Urroz, Jimena Canales, Rodrigo Troncoso, Sergio

Lavandero, Guillermo Díaz-Araya.

4. Regulatory role of reactive oxygen species in monocytic differentiation. Guillermo López-Lluch, Sara Santa-Cruz Calvo,

M. Isabel Burón, F.J. Alcaín, Plácido Navas.

9.45

5. Terfenadine induces ROS-dependent autophagy and apoptosis in human melanoma cells. María D. Boyano,

Francesca Nicolau-Galmés, Aintzane Asumendi, Gorka Pérez-

Yarza, Noelia Andollo, Idoia Ortega-Martínez, Jesús

Gardeazabal, Jesús M. Careaga and Jose Luís Díaz-Pérez.

10.00

Posters 18.00-18.30

P1. 3-O-(3,4,5-trimethoxybenzoyl)-(-)-catechin, a new synthetic tea-derived compound, induces apoptosis in melanoma cells. Magalí Sáez-Ayala, Luis

Sánchez-del-Campo, Juan Cabezas-Herrera and José Neptuno Rodríguez

López.

P2. SNAIL mediates TGF-β-induced anti-apoptotic signals. Jèssica Mainez,

Sonia Vega, Esther Bertran, M Angela Nieto and Isabel Fabregat.

P3. Role of protease activity in cisplatin-induced cell viability, apoptosis and necrosis. Sandra M. Sancho-Martínez, José M. López-Novoa , Francisco

J. López-Hernández FJ.

P4. H-RAS and N-RAS modulate fibroblast migration through AKT activation. Isabel Fuentes- Calvo, José M. López-Novoa, Carlos Martínez-

Salgado.

P5. Dlk1 affects cellular proliferation by regulating the expression of the transcriptional modulators ifi202 and ifi205. María Desamparados Ruvira,

Maria Julia González, Jose Javier García-Ramírez, Jorge Laborda, Ricardo

Sánchez-Prieto, María José Ruiz-Hidalgo.

P6. Tumor necrosis factor receptors signaling via NF-κB and JNK is

required for vascular development in zebrafish. F.J. Roca, R. Espín, J.

Meseguer, V. Mulero.

P7. Cell wall components as markers of proliferation and differentiation during plant developmental and reprogramming processes. I Bàràny, B

Fadón, D Prem, MC Rodríguez-Serrano, MC Risueño, PS Testillano.

P8. Telomerase expression and telomere length drastically decrease in senescent zebrafish. Monique Anchelin, Francisca Alcaraz-Pérez, María

Moreno, María Luisa Cayuela.

P9. Autophagy is not universally required for apoptotic cell engulfment during neural development. Patricia Boya, Marián Mellén, Enrique de la

Rosa.

P10. The effect of hydroxyurea on the external granular layer of the developing cerebellum. Vanessa Molina, Mª Carmen Santa-Cruz, Joaquim

Martí and José-Pablo Hervás.

P11. Antiproliferative and proapoptotic effects of apigenin on prostate cancer cell line TRAMP-C1. Nuria Álvarez Sánchez, Julián Castillo, Obdulio

Benavente-García, Vicente Vicente Ortega.

P12. Blockade of the epidermal growth receptor in hepatocellular carcinoma cells increases the apoptosis induced by serum withdrawal or the physiological inducer TGF-beta. Laia Caja, Patricia Sancho, Conrad

Ortiz, Esther Bertran, Isabel Fabregat.

P13. The stress treatment to induce embryogenesis increases NO production and cell death in suspension and pollen cultures of barley. MC

Risueño, M Rodríguez-Serrano, I Barany, M Coronado, PS Testillano.

P14. Parietal cells show a different maturation pattern depending on their location in the gland. Juan Francisco Madrid, Laura Gómez-Santos, Francisco

Hernández, Lucio Díaz-Flores, Francisco José Sáez.

P15. Stromal cell proliferation and differentiation during corneal wound healing. P. Gallego, C. Martínez-García, T. Blanco, L. Ibares, R. Cantalapiedra,

J. Merayo-Lloves.

P16. Detection of mitotic catastrophe by means of automated processing of fluorescence images. Santiago Rello-Varona, Oliver Kepp, Ilio Vitale, Laura

Senovilla, Mohamed Jemaà, Maria Castedo and Guido Kroemer.

8.00

Session V: Cytoskeleton and membrane traffic ROOM B

Session chairs: María del Mar Malagón (University of Córdoba) and Francisco

Wandossel (CBM, Madrid).

Invited speakers:

Gustavo Egea (University of Barcelona):

Role of actin dynamics in the structural organization of the Golgi Apparatus

8.00

Nicolás Vitale (Institut des Neurosciences Cellulaires et

Intégratives, CNRS and Université de Strasbourg, France):

Lipid dynamic in the course of regulated exocytosis

8.30

Oral communications

1. Interactions of human cytomegalovirus with cellular membranes. Victoria Cepeda and Alberto Fraile-Ramos.

9.00

2. Role of LPP3 in the structural and functional organization of the Golgi complex. Enric Gutiérrez, Inés

Fernández-Ulibarri, Francisco Lázaro-Diéguez, Elisabet Sarri,

and Gustavo Egea.

9.15

3. Identification of a novel structural motif for endo-lysosomal targeting. Dolores Pérez-Sala, Konstantinos

Stamatakis and Patricia Boya.

9.30

4. Characterization of a novel long coiled-coil protein, NECC2, in neuroendocrine cells: interaction with the neurotrophin receptor TrKA. Alberto Díaz-Ruiz, Yoana

Rabanal, Juan R. Peinado, Yolanda Jiménez-Gómez, David

Cruz-García, Rafael Vázquez-Martínez, María M. Malagón.

9.45

5. Inducible cortactin phosphorylation requires N-WASP. Elvira Nieto-Pelegrín, Eugenia Meiler, Narcisa Martínez-Quiles.

10.00

Posters 18.00-18.30

P17. Motor proteins and microtubule cytoskeleton manage ERGIC’s morphology and mobility. Emma Martínez-Alonso, Mónica Tomás, Luís A.

Martínez-Marín and José A. Martínez-Menárguez.

P18. Role of the diacylglycerol used as a substrate for the synthesis of phosphatidilcholine in the secretory pathway. Adrià Sicart, Gustavo Egea

and Elisabet Sarri.

P19. Endocytosis is altered by chronic alcohol exposure in cultured neurons. María Pilar Marín, Guillermo Esteban-Pretel, Xavier Ponsoda, Ana

María Romero, Raúl Ballestín, Carlos López, Luis Megías, Joaquín Timoneda,

Asunción Molowny, Jaime Renau-Piqueras.

P20. Ethanol decreases RhoA-GTP levels in rat astrocytes: involvement of a RhoAGAP protein. Javier Selva, Susana E. Martínez and Gustavo Egea.

P21. The role of spectrin and ankyrin isoforms in the structural and functional organization of the Golgi apparatus. Laia Salcedo-Sicilia, Susana

Granell, Cecilia Colonna, Gustavo Egea.

P22. Endocytic internalization of two nanomolecules with therapeutic potential. Jorge Soriano, Marta Díaz, Juan Carlos Stockert, Angeles

Villanueva, Magdalena Cañete.

P23. The small GTPase Rab18 and its role in the regulation of the cellular activity of adipocytes. Marina R.Pulido, Yolanda Jiménez-Gómez, Juan R.

Peinado, María Ortega, Socorro García-Navarro, Rafael Vázquez-Martínez,

María M. Malagón.

P24. The small GTPase Rab18 is part of the microtubule-based secretory granule transport machinery. Rafael Vázquez-Martínez, Marina R. Pulido,

María Ortega, Farid Almabouada, Francisco Gracia-Navarro, Socorro García-

Navarro y María M. Malagón.

P25. Esr-like proteins are secreted in maize embryogenic cultures and its inhibition by tunicamycin blocks in vitro pollen embryo development. PS

Testillano, MJ Coronado, AM Thierry, B Fadón, E Matthys-Rochon, C Dumas,

MC Risueño.

P26. Changes in the actin cytoskeleton in mouse embryonic fibroblasts lacking the antioxidative enzyme NQO1. Laura Jódar-Montilla; José A.

González-Reyes; Rafael Vázquez-Martínez and José M. Villalba.

P27. The yeast p24 complex coordinates lipid remodeling and ER exit of GPI-anchored proteins. Guillaume Castillon, Auxiliadora Aguilera-Romero,

Sharon Epstein, Javier Manzano, Reika Watanabe, Howard Riezman, Manuel

Muñiz.

P28. The interaction of human cytomegalovirus with the SNARE protein syntaxin 3. Victoria Cepeda and Alberto Fraile-Ramos.

P29. Identification of a Long Coiled-Coil Protein, NECC1, as a Novel Component of the Golgi Complex Involved in the Regulation of Protein Traffic. David Cruz-García, Alberto Díaz-Ruiz, Yoana Rabanal, Justo Castaño,

Rafael Vázquez-Martínez, María M. Malagón.

10.15

Plenary lecture

Philip N.Benfey (Duke University, Durham, NC, USA): Development rooted in interwoven networks

ROOM A

11.45

Session VI: Cellular and Molecular Pathology ROOM A

Session chairs: Jaime Renau-Piqueras (Centre of Research Hospital La Fe,

Valencia) and Amelia Sánchez Pina (CEBAS, Murcia).

Invited speakers

Erwin Knecht (Centre of Research Príncipe Felipe, Valencia):

Proteasomes and autophagy in human diseases 11.45

Julio Salinas (CIB-CSIC, Madrid):

New intermediates in Arabidopsis response to abiotic stress

12.15

Oral communications

1. Three-dimensional mitochondrial networks in plant meristematic cells: What for? José M. Seguí-Simarro.

12.45

2. MidA/PRO1853 is a putative mitochondrial methyltransferase required for complex I function. Sergio

Carilla-Latorre; M. Esther Gallardo; Sarah Annesley; Javier

Calvo-Garrido; Osvaldo Graña; Alfonso Valencia; Rafael

Garesse; Paul Fisherr and Ricardo Escalante.

13.00

3. Extracellular zinc intake in cultured astrocytes is altered by ethanol exposure. Xavier Ponsoda, Raul Ballestín,

Asunción Molowny, Maria Pilar Marín, Guillermo Esteban-

Pretel, Ana María Romero, Jaime Renau-Piqueras, Carlos

Lopez-Garcia.

13.15

4. Single-mitochondria analysis of organelle turnover, population dynamics and matrix proteases. Ivett Bárány,

Ana Navarro, MCarmen Risueño, Pilar S Testillano, JoseM

López-Cepero.

13.30

5. Dietary modulation of ghrelin cell density in a model of diet-induced obese Wistar rat. Martín-Rodríguez M, Gómez-

Ambrosi J, Muñoz-Navas MA, Frühbeck G and Burrell MA.

13.45

Posters 18.00-18.30

P30. Ethanol perturbs the Golgi complex structure during neural differentation. Mónica Tomás, Mª Pilar Marín, Emma Martínez-Alonso,

Guillermo Esteban-Pretel, Jaime Renau-Piqueras, Jose A. Martínez-

Menárguez.

P31. Golgi complex fragmentation in Parkinson´s disease. Wilson Rendón,

Mónica Tomás, Emma Martínez-Alonso, José Sánchez-Villalobos and José A.

Martínez-Menárguez.

P32. Ubiquitin-positive protein aggregates and autophagy dysfunction in Dictyostelium cells lacking Vmp1. Javier Calvo-Garrido and Ricardo

Escalante.

P33. The mitochondrial fusion protein mitofusin 2 modulates hypertrophy in cultured cardiac myocytes. Pennanen C, Morales C, Parra V, Chiong M,

Lavandero S.

P34. Adenocarcinoma prostate cancer induces hematopoietic stromal transformation. Sonia Emperador, Clara Alcaine, Fanny de la Iglesia,

MªAngeles Roy, Ana Gascon, Elena Sinues, Blanca Conde.

P35. Photoprotective effect of carnosic acid and apigenin in UV-irradiated mice. Nuria Álvarez Sánchez, Julián Castillo, Obdulio Benavente-García,

Antonio Cano Gómez, José Victor Bolarín Lucas, Vicente Vicente Ortega.

P36. Thevetia Peruviana cardiotoxic aqueous extract and its effects on chicken embryo experimental model: in vivo and in vitro response. Marco

A. Álvarez., Sequera Diana., José M. Ercolino., Lourdes Perdomo.

P37. Altered growth and RAS/ERK1/2 signaling in primary cultured vascular smooth muscle cells from cirrhotic rats. Noemí M. Atucha, Antonio

J. Ortiz, Eduardo Ortiz, Paola A. Romecín, José E. Millán, Ana B. Meseguer,

Esther M. García, Joaquín García-Estañ, María Clara Ortiz.

P38. Effect of diabetic state on GLP-1 expression in gut and serum of NOD mice. Raquel Alcaraz, Izaskun Imbuluzqueta, Carmen Beorlegui, Ana Cristina

Villaro, Mª Pilar Sesma, Mª Elena Bodegas.

P39. Changes in dermal fibroblast of young and aged rats following the application of one radiofrequency session. Esther Beltrán-Frutos, Vanesa

Sánchez-Sancha, Adelina Zuasti, Concepción Ferrer, Sergio Navarro, Vicente

Seco-Rovira, Manuel Canteras, Luis Miguel Pastor.

P40. Ultrastructural analysis of androgenic rapeseed microspores preserved by high pressure freezing and freeze-substitution. Patricia

Corral-Martínez and José M. Seguí-Simarro.

11.45

Session X: Stem cells and cellular therapy ROOM B

Session chairs: José Becerra (University of Málaga) and Salvador Martínez

(Miguel Hernández University, Alicante).

Invited speakers

Salvador Martínez (Miguel Hernández University, Alicante):

Neurotrophic potential of adult stem cells: leukodystrophy model

11.45

Augusto Silva (Dirección General de Terapias Avanzadas y

Transplantes, Ministerio de Sanidad y Política Social)

Terapias avanzadas: un reto europeo

12.15

Oral Communications

1. Dental pulp stem cells as candidates for tissue engineering. Luis Blanco Jerez, Carolina Díez Quijano, Nerea

Sánchez Pérez, Alicia Ardanuy Pizarro, and José Antonio

Uranga Ocio.

12.45

2. Cancer stem cell self-renewal inhibition: the new anti-neoplasic and relapse therapies. C. Ramírez-Castillejo. C.

Gil-Gas; P. Castro-García; C.B. Álvarez-Simón; P. Honrubia,

Jose Maria García Bueno.

13.00

3. Do silica-based ceramics hold a potential for biomedical applications? In vitro evaluation of HA-SBA-15 biocompatibility. Leonor Santos-Ruiz, Daniel Amat, Mª

Aránzazu Díaz, José Becerra.

13.15

4. Photodynamic therapy activates follicular stem cells in mouse skin. E. Carrasco, J. Espada, A. Zamarrón, M.I. Calvo,

A. Blázquez-Castro, M. Matabuena, A. Juarranz.

13.30

5. Characterization of cancer stem cells in peripheral blood from metastatic colorectal cancer patients. P. Honrubia; C.

Gil-Gas; P. Castro-García; C.B. Álvarez-Simón, Ramirez-

Castillejo C.

13.45

Posters 18.00-18.30

P41. Expression of progenitor cell markers in the development of human hair follicle. E. Poblet, P.J. Benito-Castellanos, J. Chiarri-Rodrigo R. Vera

Berón, A. Arriaga-Aragón.

P42. A cancer stem cell in the C6 glioma cell line. Gilgas C, Castro-García

P, , Honrubia P, Alvarez-Simón CB, Ferre J,Sabater S, Ramirez-Castillejo C.

P43. The premature senescence in SAMP8 mouse and its relation between shortage present in the self-regulatory signal neural stem cells. P. Castro-

García; C.B. Álvarez-Simón; F.J. Fernández-Gómez; C. Gil-Gas; J. Jordán-

Bueso; C. Ramírez-Castillejo.

P44. A 3D culture system selects non-adherent cells with mesenchymal characteristics. Silvia Claros, José A. Andrades, José Becerra.

P45. A collagen-targeted rhBMP-2 to improve bone formation in vivo. Arrabal PM, Visser R, Becerra J, Cifuentes M.

P46. Effect of pigment epithelial-derived factor (PEDF) and a PEDF-derived peptide on breast cancer cell survival. Sebastià Sabater, Carmen Belen

Alvarez Simón, Carmen Gil Gas, Paola Castro García, Carmen Ramírez

Castillejo.

15.45

Session VIII: Nucleus and gene expression ROOM A

Session chairs:

María-Carmen Risueño (CIB, Madrid) and Pilar Testillano (CIB, Madrid).

Invited speakers

Marco Biggiogera (University of Pavia, Italia ):

Changes in transcriptional activity induce alterations in the nuclear functional architecture

15.45

Ana Caño-Delgado (CSIC-CRAG, Barcelona):

Systems Biology to understand the role of plant steroid hormones in vascular development

16.15

Oral communications

1. Gene expression profiles of embryogenic competence and pollen embryogenesis development by transcriptomics and in situ hybridization. M Rodríguez-Serrano, MC Risueño,

PS Testillano.

16.45

2. Small RNAs with major roles in plant hormone networks. Ignacio Rubio-Somoza and Detlef Weigel.

17.00

3. The survival of motor neuron protein is a novel sumo-1 substrate: sumoylation of K119 contributes to regulate the number and molecular composition of cajal bodies. Rocío

Bengoechea, Olga Tapia, Miguel Lafarga and María T.

Berciano.

17.15

4. The origin of variation in transcription elongation. Ricardo Pires das Neves, Lorena Andreu, Rajeev Gupta, Nick

Jones, Tariq Enver & Francisco J. Iborra.

17.30

5. Nuclear actin binding proteins in plants: spectrin-like proteins. Clara Pérez-Munive, Susana Moreno Díaz de la

Espina.

17.45

Posters 18.00-18.30

P47. The level of DNA methylation and its chromatin distribution pattern change during plant cell differentiation and cell reprogramming. MT Solís,

M Rodríguez-Serrano, I Barany, D Prem, MC Risueño, PS Testillano.

P48. Cell cycle and ribosome biogenesis are affected by altered gravity in young Arabidopsis seedlings grown under magnetic levitation. Ana I.

Manzano, Camelia Dijkstra, Oliver Larkin, Paul Anthony, Michael R. Davey,

Richard J.A. Hill, Laurence Eaves, Eugénie Carnero-Díaz, F. Javier Medina.

P49. Unmethylation of coilin induces its nucleolar relocalization and disruption of Cajal bodies. Olga Tapia, Rocío Bengoechea, Miguel Lafarga

and María T. Berciano.

P50. Bortezomib induces the formation of nuclear poly(A) RNA granules enriched in Sam68 and PABPN1 in sensory ganglia neurons. Iñigo

Casafont, Maria Teresa Berciano, Miguel Lafarga.

P51. Mapping telomere distribution in the Arabidopsis root. Mary-Paz

González-García, Andres Canela2, Maria A. Blasco2 and Ana I. Caño-Delgado.

15.45

Session VII: Immunobiology ROOM B

Session chairs: Ángeles García Pardo (CIB, Madrid) and Victoriano Mulero

(University of Murcia).

Invited speakers

José Yelamos López (IMIM-Hospital del Mar, PRBB,

Barcelona):

Signalling pathways mediated by poly(ADP-ribose) polymerases in the immune system

15.45

Yolanda Carrasco (CNB, Madrid):

Modulation of B cell dynamics in response to chemokines 16.15

and antigen encounter

Oral communications

1. Epigenetic regulation of immune genes expression in the zebrafish. D. García-Moreno, J. Meseguer, V. Mulero.

16.45

2. Notch1 upregulates LPS-induced macrophage activation

by increasing NF-κB activity. Eva Monsalve, Almudena Ruiz-

García, Mª Dolores Alvarez-Gonzalez. Victoriano Baladrón,

Beatriz Sánchez-Solana, Samuel Rivero, Antonio Rubio, Jorge

Laborda and María José M. Díaz-Guerra.

17.00

3. 17α-ethinylestradiol modulates activation of the professional phagocytes of the bony fish gilthead seabream. Isabel Cabas, Sergio Liarte, José Meseguer,

Victoriano Mulero, Alfonsa García-Ayala.

17.15

4. Channels and hemi-channels in macrophage pro-inflammatory response: novel anti-inflammatory drug targets. Pablo Pelegrín.

17.30

5. Study of inflammatory response in macrophages obtained from ascites fluid. Tapia-Abellán A , Ruiz-Alcaraz

AJ, Hernández-Caselles T, Such J, Francés R, García-

Peñarrubia P, Martínez-Esparza M.

17.45

Posters 18.00-18.30

P52. Innate cytotoxic cells in teleost fish: 25 years of research. Alberto

Cuesta, José Meseguer, María Ángeles Esteban.

P53. Effects caused by 2-deoxy-D-glucose in immune parameters of seabream (Sparus aurata L.). F.A. Guardiola, R. Cerezuela, J. Meseguer,

M.A. Esteban.

P54. Evolution of negative regulation of Toll-like receptor (TLR) signaling: zebrafish MD1 is a co-receptor of TLR4 and RP105. Sergio Candel, María P.

Sepulcre, José Meseguer, Victoriano Mulero.

P55. Fishing the metalloprotease responsible for the processing of

interleukin-1β in fish. D. Angosto, G. López-Castejón, F.J. Roca, M.P.

Sepulcre, J. Meseguer, V. Mulero.

P56. Alternative macrophage stimulation re-program inflammatory macrophages to inhibit inflammasome activation and IL-1beta release through extracellular pyrophosphate. Alberto Baroja-Mazo, Gloria Lopez-

Castejón, Ana Gómez, Pablo Pelegrín.

18.30

SEBC MEETING ROOM A

FRIDAY 18TH 9.00

Session IX: Cell signalling ROOM A

Session chairs: Isabel Fabregat (IDIBELL, Barcelona) and José Carlos García

Borrón (University of Murcia).

Invited speakers

Alberto Muñoz (Centre for Biomedical Research “Alberto Sols”):

Effects of vitamin D on human colon cancer cells 9.00

Isabel Mérida (CNB, Madrid):

Role of diacylglycerol on the spatiotemporal regulation of Ras and Rac

9.30

Oral Communications

1. Role of PI3Kγ/PLC/IP3/calcium in insulin dependent-

glucose uptake in cardiac myocytes. Ariel Contreras-Ferrat,

Deisy Pivet, Constanza Carrillo, Cesar Vasquez, Enrique

Jaimovich, Amira Klip and Sergio Lavandero.

10.00

2. Biological significance of nuclear localization of Pmk1, an ERK1/2-type MAP kinase from fission yeast. Alejandro

Franco, Laura Sánchez-Mir, Andrés Núñez, Teresa Soto, Jero

Vicente, Mariano Gacto, José Cansado.

10.15

3. Functional characterization of a novel protein, Necc2, in adipose tissue. Yolanda Jiménez-Gómez, Alberto Díaz-Ruiz,

Marina R. Pulido, Juan R. Peinado, Raúl M. Luque, Francisco

Gracia-Navarro, Rafael Vázquez-Martínez, María M. Malagón.

10.30

4. Oxidative damage and modulation of sphingolipids in 4-HPR-mediated antineoplasic effects. Aintzane Apraiz, Jolanta

Idkowiak-Baldys, Naiara Nieto-Rementeria, M. Dolores Boyano,

Yusuf A. Hannun, Gorka Pérez-Yarza, Aintzane Asumendi.

10.45

5. L- and S-endoglin differentially modulate biological properties in L6E9 myoblasts. S. Velasco, M. Pericacho, JM.

López-Novoa, A. Rodríguez-Barbero.

11.00

Posters 18.00-18.30

P57. Autocrine expression of TGF-β confers a mesenchymal phenotype

and migratory properties to human hepatocellular carcinoma cells (HCC). Esther Bertran, Patricia Sancho, Laia Caja, Estanis Navarro, Isabel Fabregat.

P58. In vitro analysis of the effects of HGF/Met signaling on oval cell migration and invasion. Amileth Suárez-Causado, Margarita Fernández,

Cesareo Roncero, Esther Bertrán, Valentina M. Factor, Snorri S. Thorgeirsson,

Isabel Fabregat, Aránzazu Sánchez.

P59. hSprouty2 in the TNFα-NF-κB pathway: TRAF2 and p65 implications.

CA. García-Domínguez, N. Martínez, T. Gragera-Alía, D. Retana, S.

Hernández, JM. Rojas.

P60. Alpha-folate receptor: a new leading actor in melanoma resistance to methotrexate. Luis Sánchez-del-Campo, María F. Montenegro, Magalí Sáez-

Ayala, Juan Cabezas-Herrera, and José Neptuno Rodríguez –López.

P61. Effects of folic acid treatment on platelet alterations of bile duct ligated rats. Meseguer AB, Romecin PA, Millán JE, Ortíz MC, Cragnolini F,

García E, Atucha NM, García-Estañ J.

P62. Increased platelets reactive oxigen species (ROS) production in experimental cirrhosis. Romecín PA, Meseguer AB, Millán JE, Ortíz MC,

Cragnolini F, García E, Atucha NM, García-Estañ J.

P63. MGRN1 inhibits MC1R functional coupling and provides a new pathway for signalling to the nucleus. Perez-Oliva, AB.; Olivares, C.;

Abrisqueta, M.; Garcia-Borrón, JC.;, Jiménez-Cervantes, C.

P64. Functional coupling to the MAPK/ERK1/2 pathway of MC1R in melanoma cells. Herraiz Serrano, C.; Jiménez-Cervantes, C,; Garcia-Borrón,

JC.

P65. Red fluorescent protein-aequorin fusions as improved bioluminescent Ca2+ reporters. Adil Bakayan, Cecilia Fernandez, Fernando

Picazo, Beatriz Domingo & Juan Llopis.

P66. A pH exchange assay for topology determination of GFP- or YFP-tagged membrane proteins. Beatriz Domingo, María Gasset, Antonio Serrano,

Thierry Fischer and Juan Llopis.

9.00

Session XI: Biology of reproduction and development ROOM B

Session chairs: Luis Miguel Pastor (University of Murcia).

Invited speakers:

Luis Puelles (University of Murcia):

Neuronal genoarchitecture: concept and examples 9.00

Juan José Calvete (Institute of Biomedicine, Valencia):

Spermadhesins and porcine reproduction 9.30

Oral communications

1. Binder of sperm 1 is involved in the sperm binding to the zona pellucida in the bovine species. Turmo D, Mondéjar I,

Grullón L, Calvete JJ, Manjunath P, Coy P, Avilés M.

10.00

2. Activation of WNT pathway induces changes in tooth morphogenesis and cell differentiation. Aurrekoetxea M,

Ibarretxe G, Unda FJ.

10.15

3. Molecular evidences of the presence of ZP4 in the subfamily murinae. Izquierdo-Rico MJ, Laudet V, Ballesta J,

Avilés M, Chevret P.

10.30

4. Morphological, proliferative and apoptotic changes in seminiferous epithelium during testicular regression of the syrian mamster (Mesocricetus auratus) exposed to short photoperiod. Seco-Rovira V., Sánchez-Sancha V., Beltrán-

Frutos E., Zuasti A., Ferrer C., Canteras M., Pastor LM.

10.45

5. Minor ontogenetic changes in pollen development are key events for aggregated pollen shedding in the early angiosperm Annona cherimola. Jorge Lora, Pilar S.

Testillano, Maria.C. Risueño, Jose I. Hormaza, María Herrero.

11.00

Posters 18.00-18.30

P67. Protein tyrosine phosphorylation pattern in capacitated human spermatozoa in patients who failed in artificial insemination. Mª Teresa

Zomeño-Abellán, Juan Carlos Martínez, José Landeras, Irene Stetson, José

Ballesta, Manuel Avilés.

P68. Does immobilization of human sperm before ICSI induce DNA damage? EM García, MJ Gómez-Torres, J Guerrero, J Ten, R Bernabeu and J

De Juan.

P69. Immunohistochemical and ultrastructural study of the Leydig cell during aging in the syrian hamster (Mesocricetus auratus). Esther Beltrán-

Frutos, Concepción Ferrer, Adelina Zuasti, Víctor Gómez-Mayordomo, Manuel

Canteras, Vicente Seco-Rovira, Luis Miguel Pastor.

11.45

Round-table meeting: Scientific and teaching career ROOM A

Organizador: José María López Cepero (University of Cadiz).

Invited speakers

Santiago Rodríguez de Córdoba (CIB, Madrid)

Ana Caño-Delgado (CSIC-Centre for Research in Agricultural Gemonics,

CRAG)

Màrius Rubiralta i Alcañiz (Secretario General de Universidades)

15.45

Session III: Cell cycle and cancer ROOM A

Session chairs: Amparo Cano (Instituto de Investigaciones Biomédicas “Alberto

Sols”) and Marcos Malumbres (CNIO, Madrid).

Invited speakers

Cayetano González (IRB Barcelona):

Neural stem cell polarity and malignant growth in Drosophila

15.45

Raul Méndez (Centre for Genomic Regulation, Barcelona):

Mitotic cell cycle progression and tumour development are regulated by CPEB1 and CPEB4-dependent translational control

16.15

Oral communitations

1. The dual EGFR/Her-2 tyrosine kinase inhibitor Lapatinib reduces lung cancer growth through apoptosis induction. Roque Díaz, Paul A. Nguewa, Ricardo Parrondo, Carlos Perez-

Stable, Irene Sáenz de Tejada, Miriam Redrado, Raúl Catena,

María Collantes, Iván Peñuelas, Juan Antonio Díaz-González,

Alfonso Calvo.

16.45

2. Role of RACK1 ortholog in the control of G2/M transition in fission yeast. Andrés Núñez, Alejandro Franco, Laura

Sánchez-Mir, Teresa Soto, Jero Vicente, Mariano Gacto, José

Cansado.

17.00

3. Is resveratrol inducing a metabolic switch on tumoral cells? Effects of this polyphenol on cell cycle progresión. Sara Santa-Cruz Calvo, Rafael de Cabo, Plácido Navas,

Guillermo López Lluch.

17.15

4. Serum proteomic pattern of cutaneous melanoma and relation to malignant progresión. Idoia Ortega-Martínez,

Jesus Gardeazabal, Bruno Blaya, Ana Sánchez, Jose L.Diaz-

Ramón, Jesus Mª.Careaga, Rosa Izu, Aintzane Asumendi,

17.30

Gorka Perz-Yarza, María D.Boyano.

5. Effects of photodynamic therapy on human endothelial cells. M.I. Calvo, J. Espada, E. Carrasco, A. Zamarrón, M.C.

Iglesias-de la Cruz, A. Juarranz, F. Sanz-Rodríguez.

17.45

Posters 18.00-18.30

P70. Biological and genetic analysis of three new human rhabdomyosarcoma cell lines, with emphasis on the CXCR4/CXCL12 chemokine axis. Rosario Gil-Benso, Teresa San Miguel, Robert Callaghan,

Sandra Pinto, Alicia Martínez-Romero, Antonio Ferrández, Javier Boix, Juan C

Cigudosa, Concha López-Ginés.

P71. Novel pattern of EGFR amplification in glioblastoma and the relationship with gene copy number and gene expression profile. Rosario

Gil-Benso, Ruben Ferrer-Luna, Rafael Benito, Robert Callaghan, Jose

Gonzalez-Darder, Vicente Quilis, Daniel Monleon, Bernardo Celda, Teresa San

Miguel, Miguel Cerdá-Nicolas and Concha Lopez-Gines.

P72. Expression of cancer related genes in liver of turbot (Scophthalmus

maximus) exposed to carcinogenic environmental pollutants. Pamela Ruiz,

Amaia Orbea, Miren P. Cajaraville.

P73. Morphological and actin microfilament alterations induced in HeLa

cells by a new effective protocol of photodynamic therapy. Acedo P.; Rello-

Varona S.; Stockert J.C.; Cañete M. and Villanueva A.

P74. Oxidative stress and cell cycle deregulation in human melanoma.

Yoana Arroyo-Berdugo, Santos Alonso-Alegre, Aintzane Asumendi, Francesca

Nicolau-Galmés, Noelia Andollo, Neskuts Izagirre, Gorka Pérez-Yarza,

Concepción de la Rúa, María D. Boyano.

P75. Photodynamic therapy with methyl aminolevulinate and red light prevents the appearance of skin tumors induced by exposure to uv radiation. A. Zamarrón, A. Juarranz, E. Carrasco, M.I. Calvo, M. Matabuena, A.

Blázquez-Castro, F. Sanz-Rodríguez

15.45

Session IV: Cellular and Molecular Neurobiology ROOM B

Session chairs: Miguel Lafarga (University of Cantabria) and Manuel Vidal Sanz

(University of Murcia).

Invited speakers

José Ramón Naranjo (CNB, Madrid):

DREAM dominant negative mutants and mechanisms of pain control

15.45

Carlos Belmonte (Instituto de Neurociencias, Universidad

Miguel Hernández):

Converting cold into pain

16.15

Oral communications

1. Characterization of the mode of migration of microglial cells in organotypic cultures of developing quail retina. Carrasco MC, Navascués J, Calvente R, Sierra A, Martín-Oliva

D, Ferrer-Martín RM, Santos AM, Cuadros MA, Marín-Teva JL.

16.45

2. Loss of synaptic connectivity induced by the neurotoxic rotenone in the rat retina. Julian Esteve Rudd, Laura

Fernández Sánchez, Pedro Lax, José Martín Nieto, Nicolás

17.00

Cuenca.

3. DNA damage induces chromatin repositioning, telomere clustering and chromatin silencing in Purkinje cells of PCD mutant mice. Fernando C. Baltanás, Eduardo Weruaga, José

R. Alonso, Miguel Lafarga and María T. Berciano.

17.15

4. Colinearity of Hox gene expression in the mouse medulla oblongata. L. Tomas-Roca, R. Corral-San-Miguel, P. Aroca, L.

Puelles, F. Marin.

17.30

5. The clock genes: molecular cloning and characterization. Jose A, Sánchez Férez, Ana del Pozo, F. J. Sánchez-Vázquez.

17.45

Posters 18.00-18.30

P76. Analysis of irx4 expression during development chik hindbrain. Rubén Corral-San Miguel, Laura Tomás-Roca, Faustio Marín, Beatriz Lorente-

Cánovas, Pilar Aroca.

P77. The antiapoptotic TUDCA protects against mitochondrial dysfunction and synaptic contact loss in retinal degeneration. Laura Fernández

Sánchez, Pedro Lax, Gema Esquiva, Isabel Pinilla, José Martín Nieto, Nicolás

Cuenca.

P78. Mechanisms controlling the intracortical migration of gabaergic interneurons. R. Pla, V. Borrell and O. Marín.

P79. Implication of caleosins in lipid metabolism during the germination of the olive (Olea europaea L.) pollen grain. Krzysztof Zienkiewicz, Antonio J.

Castro, Juan de Dios Alché, Agnieszka Zienkiewicz, Cynthia Suárez, María

Isabel Rodríguez-García

18.30

Closure lecture: María Blasco (CNIO, Madrid):

Role of telomeres in cancer and aging