YOGI VEMANA UNIVERSITYDepartment of Environmental Science

Kadapa, A.P. - 516003, INDIA

Dr.T.Chandrasekhar Mobile: 9573154641Assistant Professor Fax: 08562-225419Principle Investigator, CSIR Project E.mail: tcsbiotech@gmail.comNo: YVU/ENVI/TCS-CSIR/Cont./II year/2013-14 Date: 11-11-2013

Quotations invited

To

Sir,

Sub: Department of Environmental Science - CSIR Project, IInd year grants, - quotations invited for chemicals, glassware, and plasticware -reg.

With references to cited above, you are requested to send your lowest quotations in triplicate in the name of Registrar, Yogi Vemana University, Kadapa-516003, YSR (Dt), A.P., India by quoting the reference YVU/ENVI/TCS-CSIR/Cont./II year/2013-14 towards the supply of the following chemicals, glassware and plasticware (list is enclosed) along with your terms and conditions so as to reach The Registrar office on or before 05-12-2013 in the sealed cover by speed or register post only.

You are also requested to send the catalog if any. Quotation includes unit price, taxes, warranty, validity of quotation and transport etc.

Thanking you

Yours Sincerely

(T. CHANDRASEKHAR)

Chemicals

S.No Name of the chemical Make Catalogue.No Quantity

1 Agarose Himedia MB002-100G 100gm2 Acrylamide Himedia MB068-25G 25gm3 Bis acrylamide Himedia MB005-25G 25gm4 SDS Sodium dodesyl sulphate Himedia MB010-25G 25gm5 APS Ammonium per sulphate Himedia MB003-25G 25gm6 TEMED Himedia MB026-100ML 100ml7 Tris free base Himedia MB029-100G 100gm8 Tris Hcl Himedia MB030-100G 100gm9 Tris Himedia MB190-100G 100gm10 Glycine Himedia MB013-100G 100gm11 DTT dithiotreitol Himedia MB070-5G 5gm12 β-mercaptoethanol Himedia MB041-100ML 100ml13 Bromophenol blue Himedia MB123-5G 5gm14 Coomassie brilliant blue Himedia MB092-5G 5gm15 Methanol Himedia MB113-500ML 500ml16 Iso propanol Himedia AS068-500ML 500ml17 Glycerol Himedia MB060-500ML 500ml18 Isobutanol Himedia MB056-500ML 500ml19 Ponceau S Himedia MB096-25G 25gm20 NaCl sodium chloride Himedia MB023-500G 500gm21 NaCl sodium chloride Himedia GRM853-500G 500gm22 BSA bovine serum albumin Himedia MB083-5G 5gm23 Triton X 100 Himedia MB031-50ML 50ml24 KCl potassium chloride Himedia GRM698-500G 500gm25 Na2HPO4 sodium phosphate dibasic

anhydrousHimedia MB024-250G 250gm

26 KH2PO4 Potassium phosphate monobasic anhydrous

Himedia MB050-100G 100gm

27 EDTA Himedia MB011-500G 500gm28 DMSO Dimethyl sulphoxide Himedia MB058-100ML 100ml29 DAB Tetra amino biphenyl

tetrahyrochlorideHimedia MB121-1G 1gm

30 CTAB Himedia MB101-100G 100gm31 Chloroform Himedia MB109-100ML 100ml32 Iso amyl alcohol Himedia MB091-100ML 100ml33 HEPES Himedia MB016-25G 25gm34 RNase A Himedia MB087-100MG 100mg35 Guanidine HCl Himedia MB014-25G 25gm36 Proteinase K Himedia MB086-10MG 10mg

37 Sodium acetate Himedia MB048-100G 100gm38 Potassium acetate Himedia MB042-100G 100gm39 Ammonium acetate Himedia MB033-100G 100gm40 Ethidium bromide Himedia MB071-1G 1gm41 PVP Himedia MB102-100G 100gm42 NaOH sodium hydroxide Himedia GRM467-500G 500gm43 Boric acid Himedia GRM1224-500G 500gm44 TCA Trichloro acetic acid Himedia GRM6274-100G 100gm45 Urea Himedia MB032-500G 500gm46 Acetone Himedia AS025-2.5L 2.5lt47 CHAPS Himedia MB084-1G 1gm48 K2CO3 Himedia GRM731-500G 500gm49 Phenol Himedia MB081-100ML 100ml50 EGTA Himedia MB130-10G 10gm51 PMSF Phenyl methane sulfonyl

fluorideHimedia MB144-1G 1gm

52 PEG Polyethylene glycol Himedia MB148-500G 500gm53 GSSG oxidized glutathione Himedia TC282-100MG 100mg54 Gelatin Himedia GRM019-500G 500gm55 Citric acid Himedia MB174-500G 500gm56 Na2HPO4.2H2O Sodium phosphate

dibasic dehydrateHimedia MB126-500G 500gm

57 NBT Nitrotetrazolium blue chloride Himedia MB107-100MG 100mg58 L-Methionine Himedia PCT0315-25G 25gm59 Riboflavin Himedia PCT0214-10G 10gm60 Glacial acetic acid Himedia AS001-500ML 500ml61 5-Sulphosalicylic acid dihydrate Himedia GRM7527-250G 250gm62 Orthophosporic acid Himedia AS011-500ML 500ml63 Toluene Himedia AS074-500ML 500ml64 Proline Himedia PCT0317-25G 25gm65 Na2CO3 Himedia GRM851-500G 500gm66 Potassium sodium tartarate

tetrahydrate Himedia GRM1046-500G 500gm

67 Gamborg B5 Medium Himedia PT017-25L 25lt68 YEP Broth Himedia M1827-500G 500gm69 Kanamycin Himedia PCT1105-5G 5gm70 Rifampicin Himedia CMS1889-5G 5gm71 Cephotaxime Himedia MB134-10G 10gm72 Acetosyringone Himedia PCT1301-1G 1gm73 HiPurATMPlant DNA isolation kit Himedia MB502-50PR 50pr74 Molecular biology grade water Himedia ML024-10x100ML 500ml75 Folins reagent Himedia ML059-125ML 125ml76 Bradford reagent Himedia ML106-100ML 100ml77 Biuret reagent Himedia ML122-100ML 100ml78 Blocking buffer Himedia ML044-100ML 100ml

79 Ponceau S stain Himedia ML045-250ML 250ml80 Luria Bertaini Broth Miller Himedia M1245-500G 500gm81 2X PCR Taq mixture Himedia MBT061-20R 20r(0.5ml)82 2X PCR Taq mixture Himedia MBT061-50R 50r(1.25ml)83 Bam H1 restriction enzyme Himedia MBRE002-250U 250u84 Hind III restriction enzyme Himedia MBRE003-250U 250u85 2X Laemmli buffer Himedia ML021-5x6ML 5x6ml86 1X PBS Himedia ML116-100ML 100ml87 1X TE buffer Himedia ML060-100ML 100ml88 1Kb DNA ladder 50µg Merck 612652070501730 1 EA89 1Kb DNA ladder 100µg Merck 612652071001730 1 EA90 Protein molecular weight broad

range 50 lanes 0.5mlMerck 623110475001730 1 EA

91 Protein molecular weight broad range 25 lanes 0.25ml

Merck 623110472501730 1 EA

92 Protein molecular weight lower range 50 lanes 0.5ml

Merck 623110175001730 1 EA

93 Bam H1 2000U Merck 610100100021730 1 EA94 Hind III 4000U Merck 610100600021730 1 EA95 Red dye PCR Master mix 2X (5x10

rxn)Merck 610667800041730 1 EA

96 CTAB plant DNA extraction kit 10 preps

Merck 612105500011730 1 EA

97 EmeraldAmp® MAX PCR Master Mix 160 rxns

TaKaRa RR320A

98 TaKaRa Taq™ DNA Polymerase 250 units

TaKaRa R001A

99 Premix Taq™ DNA Polymerase (TaKaRa Taq™ Version 2.0) 150 units

TaKaRa R004A

100 Nucleospin plasmid-50 TaKaRa 740588101 Nucleo spin extract -50 TaKaRa 740609102 Not I restriction enzyme NEB103 ECL western blotting kit Thermoscientific104 X-ray film105 Cassette106 Sterlin 5lt x 10107 Ethyl alocoholAR Any make 500ml x 10108 DMSF Fisher /

Himedia/ Merck …………… …………

109 Thiourea Fisher / Himedia/ Merck

………… …………

110 SB3 Fisher / Himedia/ Merck

………… …………

111 Phospine Fisher / ………… ………….

Himedia/ Merck 112 Pharmalyte Fisher /

Himedia/ Merck ………… …………..

113 H2O2 Fisher / Himedia/ Merck

………. ……….

GlasswareS.No Name of the glassware Capacity Make & Cat No.

1 Volumetric flask 50 ml Borosil -5641 2 Volumetric flask 100 ml Borosil-5641 3 Volumetric flask 250 ml Borosil-5641 4 Volumetric flask 500 ml Borosil-5641 5 Volumetric flask 1000 ml Borosil-5641 6 Conical flask 50 ml Borosil-49807 Conical flask 100 ml Borosil-49808 Conical flask 250 ml Borosil -49809 Conical flask 500 ml Borosil -498010 Conical flask 1000 ml Borosil -498011 Measuring cylinder 10 ml Borosil-302212 Measuring cylinder 25 ml Borosil-302213 Measuring cylinder 50 ml Borosil-302214 Measuring cylinder 100 ml Borosil- 302215 Measuring cylinder 250 ml Borosil- 302216 Measuring cylinder 500 ml Borosil-302217 Measuring cylinder 1000 ml Borosil-302218 Pipette 0.1 ml Borosil-706019 Pipette 0.5 ml Borosil-706020 Pipette 1.0 ml Borosil-706021 Pipette 2 ml Borosil-706022 Pipette 5 ml Borosil-706023 Pipette 10ml Borosil-706024 Beaker 25 ml Borosil-100025 Beaker 50 ml Borosil-100026 Beaker 100 ml Borosil-100027 Beaker 250ml Borosil-100028 Beaker 500ml Borosil-100029 Beaker 1000ml Borosil-100030 Amber color Reagent bottle 50ml Borosil-151931 Amber color Reagent bottle 100ml Borosil-151932 Amber color Reagent bottle 250 ml Borosil-1519

33 Amber color Reagent bottle 500 ml Borosil-151934 Reagent bottle 50 ml Borosil-150135 Reagent bottle 100 ml Borosil-150136 Reagent bottle 250 ml Borosil-150137 Reagent bottle 500ml Borosil-150138 Reagent bottle 1000 ml Borosil-150139 Funnel 65 mm Borosil-640140 Funnel 75mm Borosil-640141 Funnel 100 mm Borosil-640142 Test tubes with rim 25x 150 mm Borosil-980043 Rimless test tubes 25x 150 mm Borosil-980044 Autoclavable Tissue culture bottles with

polystyrene cap250ml ……..

45 S-line Petridishes 100x15mm Borosil-316546 S-line Petridishes 200x30mm Borosil-3165

Plasticware

S.No Name of the item (Plasticware) Capacity Make and catalogue No

1 Amber color wide mouth reagent bottle 30ml Tarsons 5813002 Amber color wide mouth reagent bottle 60ml Tarsons 5813103 Amber color wide mouth reagent bottle 500ml Tarsons 5813404 Test tube baskets 180 x 170 x 160 Tarsons 1800205 Funnel 35mm Tarsons 6300106 Micropipette tip 0.2-10µl Tarsons 5210007 Micropipette tip 2-200 µl Tarsons 5210108 Micropipette tip 200-1000 µl Tarsons 5210209 Micro tip box 0.2-10µl Tarsons 52405110 Micro tip box 2-200 µl Tarsons 52405011 Micro tip box 200-1000 µl Tarsons 52408012 Tough tags 24 x 12.7mm Tarsons 50006013 Tough spots dia 9.5 Tarsons 50008014 Reversible rack with cover 96 places Tarsons 24101015 Centrifuge tubes conical bottom 15ml Tarsons 50003116 Centrifuge tubes conical bottom 50ml Tarsons 50004117 Centrifuge tubes round bottom 16ml Tarsons 54301018 Centrifuge tubes round bottom 50ml Tarsons 54302019 Caps 16ml Tarsons 54001020 Caps 50ml Tarsons 54002021 Oak ridge centrifuge tubes 10ml Tarsons 54102022 Oak ridge centrifuge tubes 30ml Tarsons 541030

23 Oak ridge centrifuge tubes 50ml Tarsons 54104024 Oak ridge centrifuge tubes 70ml Tarsons 54105025 Rack for microtube 1.5ml 20 places Tarsons 24001026 Universal combi rack …… Tarsons 20208027 Test tube stand 25mm tubes 18 places Tarsons 20204028 Cryo vial sterile 1.8ml Tarsons 52302029 Cryo vial sterile 4.5ml Tarsons 52303030 Cryo coder white …… Tarsons 52311031 Cryo coder blue …… Tarsons 52312032 Cryo coder red …… Tarsons 52313033 Cryo coder yellow …… Tarsons 52314034 Cryo rack 50 places Tarsons 24105035 Microtube box 1.5ml 64 places Tarsons 52407036 Cryobox 1.0 and 1.8ml 81 places Tarsons 52401037 Cryobox 4.5ml 81 places Tarsons 52403038 Cryo cube box 1.8ml 100 places Tarsons 20207039 -20 PCR minicooler 0.2ml 96 places Tarsons 52511040 Ice tray 600ml Tarsons 52508041 Float rack 1.5ml 16 places Tarsons 24000042 Float rack 1.5ml 8 places Tarsons 24004043 Nitrile gloves Small Tarsons 37201044 Nitrile gloves Medium Tarsons 37202045 Hand protector grip …… Tarsons 80000046 Planton plant tissue culture container 7.5 x 7.5 x 10 cm Tarsons 02008047 Test tube cap 25mm Tarsons 02007048 Staining box 12.5 x 12.5 x 5 cm Tarsons 48202049 PCR tubes flat lid 0.2ml Merck/ Himedia/ Tarsons

50 8 strip PCR tubes with flat cap 0.2ml Merck/ Himedia/ Tarsons

51 96 well non skirted PCR blocks 0.2ml Merck/ Himedia/ Tarsons

52 Caps for 96 well PCR blocks 0.2ml Merck/ Himedia/ Tarsons

53 Phyta jar for plant tissue cultureRound container

250ml Himedia PW1125-25NO

54 Phyta jar for plant tissue cultureSquare container

500ml Himedia PW1143-25NO

55 Sterile syringe driven filters 0.22µm with PES membrane

25mm dia Any make

56 Hidispo Bag – 14Size : 20 (h) x 14 (b)

500 no Himedia PW038-500NO

57 Freeze tag, multicolour dots 1x6000 no Himedia LA937M-1x6000NO58 Pasture pipettes 1x50 no Himedia PW1206-1x50NO59 Magnetic bar retrievers 1 no Himedia PW176-1NO60 Spatula -200 50 no Himedia LA006-50NO61 Metaloop -SS-2 1x8 no Himedia LA650-1x8NO62 Stainless steel forceps blunt 8 inches 2 no Himedia LA707-2NO63 Stainless steel forceps blunt 10 inches 2 no Himedia LA708-2NO

64 Stainless steel forceps blunt 12 inches 2 no Himedia LA767-2NO65 Stainless steel scalpel holder NO.3 2 no Himedia LA829-2NO66 Stainless steel scalpel holder NO.4 2 no Himedia LA830-2NO67 Sterile scalpel blade NO.10 1x100 no Himedia LA768-1x100NO68 Sterile scalpel blade NO.11 1x100 no Himedia LA769-1x100NO69 Sterile scalpel blade NO.15 1x100 no Himedia LA770-1x100NO70 Sterile scalpel blade NO.20 1x100 no Himedia LA771-1x100NO71 PCR tubes work station Orange colour 1x5 no Himedia CG297-1x5NO72 Parafilm 1 no HimediaLA017-1NO73 Buffer capsules ,pH:4.0 10 no Himedia LA405-10NO74 Buffer capsules ,pH:7.0 10 no Himedia LA406-10NO75 Buffer capsules ,pH:9.2 10 no Himedia LA407-10NO76 Stainless steel stand 2 no Himedia LA722-2NO77 HiIndicator pH paper 1 pk Himedia LA310-1PK78 Coombs for Electrophoresis

Mini and Midi systemNYXTECHNIK

79 Glass rods 10 No80 Glass spreaders 4 No81 Autoclave gloves 2 sets82 Autoclave biohazard covers

Small and medium size100 No

83 Pestle and mortarSmall size

10 No

84 Watmann no 1 filter papers 1 pk85 Blotting paper 1reem 86 Spirit lamps glass made 4 No87 Disposable gloves 300 No88 Cotton rolls 4 No89 Tissue rolls 1 Box

Yours Sincerely

(T. CHANDRASEKHAR)