water effluent comparison to regulation
TRANSCRIPT
Water Effluent Comparison To Regulation
Mackenzie Pulp Mill Corp.
Permit: PE01138
2020-06-16 9:28:15 AM
Period Starting: May 01, 2020
Period Ending: May 31, 2020
FLOW Actual Daily Avg (E3M3/D) 62.1
90_EFF 90th Percentile (Previous 100 Days) 62.7
PROD Actual Daily Avg (ADT/D) 488
90_PROD 90th Percentile (Previous 100 Days) 610
BL Actual Daily Avg (ADT/D) 488
90_CBPROD 90th Percentile (Previous 100 Days) 610
PRODUCTION Reference Production Rate (RPR) 705
TSS Permitted Max (mg/l) 182
Permitted Avg (mg/l) 110
Actual Daily Max (mg/l) 119
Actual Daily Avg (mg/l) 78.1
BOD Permitted Max (mg/l) 73
Actual Daily Max (mg/l) 34
Actual Daily Avg (mg/l) 23.4
AOX Permitted Avg (KG/ADT) 0.60
Actual Daily Avg. (KG/ADT) 0.26
Actual Daily Avg (mg/l) 3
Page: 1 / 1
Mackenzie Pulp Mill Corporation
Biobasin #2 - Temperature (°C)
May 2020
DATE Minimum Temperature Maximum Temperature
01-May 22.4 26.4
02-May 22.8 26.0
03-May 10.4 25.0
04-May 22.7 28.3
05-May 24.1 26.8
06-May 22.7 26.8
07-May 23.3 28.7
08-May 25.6 30.7
09-May 24.6 29.8
10-May 23.5 28.9
11-May 23.5 30.3
12-May 25.3 30.6
13-May 25.4 32.4
14-May 24.8 30.8
15-May 27.4 32.6
16-May 27.4 32.0
17-May 26.3 30.1
18-May 24.7 31.6
19-May 27.6 32.1
20-May 27.7 30.8
21-May 27.6 31.0
22-May 27.5 30.5
23-May 25.4 30.0
24-May 26.1 30.0
25-May 25.3 29.7
26-May 24.6 29.8
27-May 25.2 32.0
28-May 27.0 30.4
29-May 25.2 31.7
30-May 21.4 31.0
31-May 21.3 23.1
Monthly Min./Max. 10.4 32.6
Monthly Average 27.9
Date pH Temperature (⁰C) pH Temperature (⁰C)
01-May 7.04 22.4 7.21 19.3
02-May 6.99 23.7 7.25 21.3
03-May 7.05 24.2 7.30 21.8
04-May 6.98 25.0 7.07 22.0
05-May 6.95 26.1 7.12 19.9
06-May 6.88 25.3 7.01 21.0
07-May 6.80 27.9 6.94 24.8
08-May 6.81 28.7 7.08 24.4
09-May 6.84 25.1 7.23 22.7
10-May 6.83 26.6 7.44 23.1
11-May 6.85 27.8 7.11 23.3
12-May 6.85 27.5 7.03 25.3
13-May 6.85 29.1 7.07 25.0
14-May 6.81 28.5 7.12 23.3
15-May 6.89 29.7 7.10 25.9
16-May 6.95 28.1 7.12 28.8
17-May 6.89 29.1 7.07 24.8
18-May 6.95 29.1 7.13 24.2
19-May 6.96 30.6 7.12 27.5
20-May 6.94 29.6 7.51 26.5
21-May 6.93 29.7 7.47 28.0
22-May 6.91 29.1 7.56 26.7
23-May 6.92 26.1 7.52 24.0
24-May 6.93 26.8 7.44 25.5
25-May 6.89 28.9 7.46 24.7
26-May 6.90 27.2 7.69 23.4
27-May 6.87 28.3 7.68 24.8
28-May 6.86 29.2 7.46 26.0
29-May 6.85 29.5 7.44 25.7
30-May 6.80 29.3 7.41 18.7
31-May 6.87 28.4 7.43 26.9
Field Laboratory
May-20
Biobasin #2
pH & Temperature
Date
01-May 2060
02-May 1990
03-May 1959
04-May 1923
05-May 1893
06-May 1891
07-May 1919
08-May 1960
09-May 1928
10-May 1911
11-May 1899
12-May 1900
13-May 1601
14-May 1591
15-May 1594
16-May 1522
17-May 1532
18-May 1519
19-May 1509
20-May 1506
21-May 1510
22-May 1560
23-May 1487
24-May 1451
25-May 1429
26-May 1406
27-May 1406
28-May 1384
29-May 1370
30-May 1380
31-May 1366
Monthly Min/Max 1366 2060
Monthly Average 1657
May 2020
#2BO-24 hr-Conductivity (µS/cm)
Biobasin #2 Conductivity
Date
01-May 724
02-May 645
03-May 617
04-May 580
05-May 740
06-May 540
07-May 480
08-May 810
09-May 730
10-May 570
11-May 540
12-May 660
13-May 710
14-May 760
15-May 601
16-May 584
17-May 538
18-May 562
19-May 571
20-May 566
21-May 588
22-May 597
23-May 529
24-May 520
25-May 569
26-May 505
27-May 488
28-May 489
29-May 413
30-May 431
31-May 415
Monthly Min/Max 413 810
Monthly Average 583
May 2020
#2BO-24hr COD (mg/L)
Biobasin #2 Chemical Oxygen Demand (COD)
DATE Ammonia Ammonia Nitrate Nitrite
NH4 - N NH4 - N NO3 - N NO2 - N Dissolved Total
PO4 - P PO4 - P
01-May 1.35
02-May 1.43
03-May 0.98
04-May 0.81
05-May 0.18 0.123 <0.0500 <0.0100 5.07 0.0364 0.464
06-May 0.19
07-May 0.75
08-May 0.03
09-May 0.21
10-May 0.49
11-May 0.83
12-May 1.38
13-May 1.46
14-May 0.78
15-May 1.06
16-May 1.57
17-May 2.00
18-May 1.89
19-May 1.45
20-May 2.84
21-May 3.71
22-May 2.77
23-May 3.49
24-May 3.61
25-May 2.58
26-May 2.46
27-May 3.59
28-May 3.23
29-May 0.62
30-May 1.27
31-May 1.10
PhosphorusTotal
Kjeldahl
Nitrogen
Biobasin #2 - Nutrients (mg/L)
May 2020
Tested In-
houseTested by ALS Environmental
8664 Commerce Court, Burnaby, BC V5A 4N7
Acute Toxicity Test Results
Sample #2BO – GRAB – 05/05/2020,
collected May 05, 2020
Final Report
May 21, 2020
Submitted to: Mackenzie Pulp Mill Corporation
Mackenzie, BC
WO#200776 – 200777 Nautilus Environmental Company Inc. 1
SAMPLE INFORMATION
Sample ID
Dates Receipt
temp. Collected Received Rainbow trout
test initiation
Daphnia magna
test initiation
#2BO – GRAB –
05/05/2020
05-May-20 at
0830h
06-May-20
at 0915h
07-May-20 at
1750h
06-May-20 at
1505h 14.4°C
TESTS
• Rainbow trout 96-h LC50 test
• Daphnia magna 48-h LC50 test
RESULTS
Toxicity test results
Sample ID LC50 (% v/v)
Rainbow trout Daphnia magna
#2BO – GRAB – 05/05/2020 >100 >100
LC = Lethal Concentration
QA/QC
QA/QC summary Rainbow trout Daphnia magna
Reference toxicant LC50 (95% CL)
97.8 (72.9 – 131.1) µg/L Zn1
5.5 (4.4 – 6.9) g/L NaCl2
Reference toxicant historical mean
(2 SD range) 100.9 (54.1 – 188.3) µg/L Zn 6.2 (4.7 – 8.2) g/L NaCl
Reference toxicant CV 32% 14%
Organism health history Acceptable Acceptable
Protocol deviations None None
Water quality range deviations None None
Control performance Acceptable Acceptable
Test performance Valid Valid
1Test Date: April 13, 2020;
2 Test Date: May 06, 2020; LC = Lethal Concentration, CL = Confidence Limits, SD =
Standard Deviation, CV = Coefficient of Variation
WO#200776 – 200777 Nautilus Environmental Company Inc. 2
Report By: Reviewed By:
Ditty Kakkassery, R.P. Bio Andy Diewald, B.Sc.
Laboratory Biologist Senior Analyst
This report has been prepared by Nautilus Environmental Company Inc. based on data and/or samples
provided by our client and the results of this study are for their sole benefit. Any reliance on the data by a
third party is at the sole and exclusive risk of that party. The results presented here relate only to the
samples tested.
APPENDIX A – Summary of test conditions
Table 1. Summary of test conditions: 96-h rainbow trout (Oncorhynchus mykiss)
LC50 test.
Test species Oncorhynchus mykiss
Organism source Hatchery
Organism age Juvenile
Test type Static
Test duration 96 hours
Test vessel 20-L glass aquarium
Test volume 10 to 20 L (depending on size of fish)
Test solution depth ≥15 cm
Test concentrations Five concentrations, plus laboratory control
Test replicates 1 per treatment
Number of organisms 10 per replicate
Control/dilution water Dechlorinated Metro Vancouver municipal tapwater
Test solution renewal None
Test temperature 15 ± 1°C
Feeding None
Light intensity 100 to 500 lux
Photoperiod 16 hours light / 8 hours dark
Aeration 6.5 ± 1 mL/min/L
Test measurements
Temperature, dissolved oxygen and pH measured daily;
salinity measured in the undiluted sample at test initiation;
conductivity measured at test initiation and termination;
survival checked daily
Test protocol Environment Canada (2000), EPS 1/RM/13, with 2007 & 2016
amendments
Statistical software CETIS Version 1.9.4
Test endpoint Survival (96-hour LC50)
Test acceptability criterion for controls Survival ≥90%
Reference toxicant Zinc (added as ZnSO4)
Table 2. Summary of test conditions: 48-h Daphnia magna LC50 test.
Test species Daphnia magna
Organism source In-house culture
Organism age <24-hour old neonates
Test type Static
Test duration 48 hours
Test vessel 250-mL glass beaker
Test volume 200 mL
Test solution depth 6 cm
Test concentrations Five concentrations, plus laboratory control
Test replicates 1 per treatment
Number of organisms 10 per replicate
Control/dilution water Moderately-hard reconstituted water + 2.5 µg/L Se
Test solution renewal None
Test temperature 20 ± 2°C
Feeding None
Light intensity 400 to 800 lux
Photoperiod 16 hours light / 8 hours dark
Aeration None
Test measurements
Temperature, dissolved oxygen and pH measured daily;
salinity, hardness and alkalinity measured in the undiluted
sample at test initiation; conductivity measured at test
initiation and termination; survival checked daily
Test protocol Environment Canada (2000), EPS 1/RM/14, with 2016
amendments
Statistical software CETIS Version 1.9.4
Test endpoint Survival (48-hour LC50)
Test acceptability criterion for controls Survival ≥90%
Reference toxicant Sodium chloride (NaCl)
APPENDIX B – Toxicity test data
Rainbow Trout Summary Sheet
Client:
Work Order No.:
Sample Information:
Sample ID:
Sample Date:
Date Received:
Sample Volume:
Other:
Dilution Water:
oii'2BO - CrmJ,O - cs f~sl2,2o
M "'ij os- . ?o 20
Test Species: Oncorhynchus mykiss
Test Validity Criteria:
~ 90% control survival
WQRanges:
T CC) = 15 ± 1; DO (mg/l) = 7.0 to 10.3; pH= 5.5 to 8.5
Type: Dechlorinated Municipal Tap Water
Hardness (mg/L CaC03): ID Alkalinity (mg/L CaC03):
Test Organism Information:
Batch No.: 03il20a
Source:
No. FishNolume (L):
Loading Density (g/L):
Mean Length ± SD (mm): Range: 3 3 - 1; '5
Mean Weight± SD (g): Range: o·36 - 1-ol
Zinc Reference Toxicant Results:
Reference Toxicant ID:
Stock Solution ID: 19U.o5 Date Initiated:
96-h LC50 (95% CL): qu { 72.9- i3i.l) "?''- 2'1
Reference Toxicant Mean and Historical Range:
Reference Toxicant CV (%): 21.
Test Results:
Reviewed by:
Version 1.4; Issued May 29, 2015.
96-Hour Rainbow Trout Toxicity Test Data Sheet
Client/Project#:
Sample I. D.
W.O.#
RBT Batch#:
Date Collected/Time:
Date Setup/Time:
CER#:
Sample Setup By:
Thermometer:~
Mnvl<i'.r,"i,Q, Rtlp tl\.\\ CoC(lo~tJ1oD it ·z&J- 61 Q/1£- o'f!o;r f7&7£J - --
IJ1_
;- ... ' D.O. meter/probe:~ P::L j Cond./Salinity meter/probe: .(Z I Cl'Z- 5' pH meter/probe: ('2::: 1 ?:~ } P -p-
,.... ""
'"" Number FishNolume: ...L\li.L)-"/~"""'.::c...:l.::e:s'c_'- ______ _
7-d% Mortality: ....,,--,....,,-_i:.:·"=-:--------Total Pre-aeration Time (mins): #~ i I 0 __ Aeration rate adjusted to 6.5 ± 1 mL/min/L? (Y/N): _!.\ -----
Undiluted Sample WQ
Parameters lnitiaiWQ Adjustment 30 minWQ
Temp 'C 15'.s / /{.('
D.O. (mg/L) 3.1 / 5"3 pH 1.7.. / 1.~ Cond. (~S/cm) 1?1>7 .. / n9t Salinity (ppt) 0.') I 0."'
Concentration #Survivors Temperature ('C) Dissolved Oxygen (mg/L) pH Conductivity
(IJ (~S/cm)
(% v/v) 1 2 4 24 48 72 96 0 24 48 72 96 0 24 48 72 96 0 24 48 72 96 0 96
(Jr \ I ~C. 10 vo q tq.o 1·>.o IS II ,,_0 iS.o I(~ 'l.\l % q,'f Cf,J 1:z. '"\.1. I.) 7:1.. '1.1. Jz. 3'1 b~"Z.S"' \0 lo /O {0 1'~- ))'.o (S,D ~-0 Ito C!;-1 cl.\ 92 lq,q 'I'{ r··z.. 1-4 7>?.
-~--'1.3 1'11 15"2-
11..) \ll /0 /.0 10 ~~-\) \)--:'0 t~":>{) 4".0 IS!) elf! "l.(, "/:7 14.'6 9.{ "1.1 .,,, IS 1.~ :l& 1.'3B 2'-l'f ?.~ \0 ;0 '1-0 IO pL) IS. v 15-0 I c;. o IS.D Ci.~ ·~.~
9(, lti-~ q_<; "1 .":( 1.<1 g,., /."/ 'f.[ 1.{"1] L(qq
So 1\l lO lO IQ \o.\~ 1->.0 /•;,v /P 1\.D q,J "1:1 c.; .c. lq.;r q_b i.Y i '6 -\ £_,,, I z:t. a.; "lil'\ 91/ IOO l\l (0 10 1\) r:::\) li:,; !5o li<;.o 1\.o ,,'2 q.~ ').L q.1 '1-( '1S IR.t. £~l g.s- '$.~ W1B 11os'
Initials #- ~1"1 iU'f It<- !lA- L,.c: ','V/ I wilY M.. -c.z Svi'J )l.!lf' ,...__
"'"' t'-7- !o,f-1 lftt\(" ;t-v A- ,.,_
" 1..rh,J , ll:v"'h ~y1J 1
_,
l!::f;lfl mdl atfe;u, hf9'-"h aurhw.ltttfCJ I ' I
Fish Description at 96 h ~"'«~'''''' f,,~ ~Pfl"' M (""'..( Number of Stressed Fish at 96 h /
Other Observations: (/) w..eJ tJ/d - 6 . f
Reviewed by: ---------"''----- Date Reviewed: fnJ I y '/..'>
Version 2.5; Issued July 19, 2017 Nautilus Environmental Company Inc.
Client:
Work Order No.:
Sample Information:
Sample ID:
Sample Date:
Date Received:
Sample Volume:
Daphnia magna Summary Sheet
~:ffhz/-G I!.+ tftti Start Date/Time II"'? ti-,& .... e ts-ori. Z-<>o 7-7-(. C., fhr..::Pu•. Test Species: ---'6~aapo:;h"n!.!:ia"-!.!m!.!:a~g~n"a ___ _
Set up by: __ £J.,@<t!11f:r:~_,.'----
Test Validity Criteria:
;::. 90% mean control survival and/or mobility and :52
:t:IZ.8o - 6,.J;- os-,kd:z..-z,., daphnids exibit immobility and/or mortality in any
&p e~/-zoz, ' single control replicate.
Ap ot,/~ ""' WQ Ranges:
'Z../ z.,t.. T CC) = 20 ± 2; DO (mg/L) = 3.6 to 9.4; pH= 6 to 8.5
Test Organism Information:
Brood stock No.:
Age of young (Day 0): <24 h
Avg No. young per brood in previous 7 d: 6 Mortality(%) in previous 7 d:
Days to first brood:
NaCI Reference Toxicant Results:
Reference Toxicant ID:
Stock Solution ID:
Date Initiated:
48-h LC50 (95% CL):
;il>f Reference Toxicant Mean and Historical Range: f,. Z GrY.r-q.z.)rL NaCL Reference Toxicant CV (%): ____ ..!to:J'ft_ _________ _
Test Results:
Reviewed by: Date reviewed: ---):..._+_':...{1-Y-==---
Version 1.5~ Issued Sep. 30,2015 Nautilus Environmental Company Inc.
Freshwater Acute
48 Hour Toxicity Test Data Sheet
Client:
Sample ID:
Work Order No.:
t!YhaV'<. /?.vf' /)j.;ij h.,--.-~-M>" Start Date/Time: ~ #6,/.-ze,ZoQ /9)_4_ :ft=u;c: - &.c.,b - o s /" ">",/ -z._z_o CER #: ----''---------
-u>o r ?= b ' No. Organisms/volume: -::-'1"'0/c=2;e;OO"'m"'L"--------
Test Organism: _,o"'.m"-a"'g'7n:':a=,----------
Set up by: /fft-P
Thermometer:({,(~-;;' pH meter/probe: _____{g__!_f,z_ DO meter/probe: _f_1~ Cond./Salinity meter/probe: _i,_;_£_
Concentration Number of No.
Temperature Dissolved oxygen pH Conductivity
Live Organisms Immobilized
("C) (mg/L) (~S/cm)
Z(vlv) Rep
24 48 48 0 24 48 0 24 48 0 24 48 0 48
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c D
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Technician Initials II,\; I!, JiA) .J\J\) !<UY IJ;«<-fl' J~~ lt.iUf ltUvf ·Jw /)l.f tiW ttJ>r df!j? /ft4f n11a us men use
Hardness" I Alkalinltv" Teme ("Cl j£{.0 '
( 1.0 Concentration •(m /Las CaCo3l DO_lmg/L) 3.'f mJ>J: J \ '(:o Control IMHWl iOCJ ~+ H ~-4 '.t.. ">IN~ ..., . .., Highest cone. L'30 Zli"- Cond (uS/em f'f'Z.o
, l-:Hb
Hardness adjusted - Salinity (ppt) n.tl Q."(
Comments:
Sample Description:
Mortality: Heartbeat checked under microscope "-<5i: ,..e '1. toi.
"'i"'bA b-.;o>-'"' ~,..;~."...:~, I>.Jp ..,;\l DMvtn. Dollw·n p~v--1.t<J:"' ·
Batch#: b12%7ol[, 7~d previous# young/brood: 2 b Previous 7~d Mortality (%): __ o ___ Day of 1st Brood: __ q_!_ __
Reviewed by: Date reviewed: ----~--""'\4' _..!.I..:::~..;.!_.'U:I,_ ____ _
Version 1.9; Issued July 19, 2017 Nautilus Environmental Company Inc.
APPENDIX C – Chain-of-custody form
TESTING LOCATION (Please Circle)
G ~~H!!~N~~ Chain of Custody
Burnaby 8664
Commerce Court Burnaby, British
Columbia, Canada VSA 4N7
Phone 604.420.8773 Date _p ---- . -"'- .
Report to: Invoice To: ANAL VSES REQI IRED
Company Mackenzie Pulp Mill Core:oration Company Mackenzie Pulp Mill Corporation
Address PO Box 2609, 1000 Coquiwaldi Rd. Address PO Box 2609, 1000 Coquiwaldi Rd. ~ City/State/Zip Mackenzie, BC, VOJ 2CO City/State/Zip Mackenzie, BC, VOJ 2CO ~ ~
~ = ~
"" Contact Randeep Sandhu Contact Kim Harnett ro
~ -e B ~
Phone 250·997·2444 Phone 250·997·2473 "' ~
~ c ro ·e s Email MPMC-Environment@mackenzieQUI():.com Email [email protected] 0 ID ~·.· ~ g; "' MPMC-T echnical@mackenzi8!2ulp.com PO No. OJ c ~
43011427 ~ ·e J ro 0 ro ID f c ~ c ~ Sample Collection By: KV/CA Sample Type: Grab X OR Composite 0 "' u "' v ~
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#OF CONTAINERS AND "' "' " SAMPLE ID DATE TIME MATRIX COMMENTS ~ ~ ~ ~ ~
VOLUME (e.g. 1 x 20 L) 00 "' 00 "' ~
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Sample confirmation redept, mortality update and report to required: 1. Total No. of . · ·.· . ·
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(Com an Com an) .· :.:·' ·._;_-_: "~ ·.- .-. ·· Date:mdTimel
Additional costs may be required for sample disposal or storage. Payment net 30 unless otherwise contracted.
END OF REPORT
8664 Commerce Court, Burnaby, BC V5A 4N7
Acute Toxicity Test Results
Sample #2BO – GRAB – 12/05/2020,
collected May 12, 2020
Final Report
May 27, 2020
Submitted to: Mackenzie Pulp Mill Corporation
Mackenzie, BC
WO#200878 Nautilus Environmental Company Inc. 1
SAMPLE INFORMATION
Sample ID
Dates Receipt
temperature Collected Received Daphnia magna
test initiation
#2BO – GRAB –
12/05/2020
12-May-20 at
0730h
13-May-20 at
1130h
13-May-20 at
1925h 8.1oC
TEST
• Daphnia magna 48-h LC50 test
RESULTS
Toxicity test results
Sample ID LC50 (% v/v)
#2BO – GRAB – 12/05/2020 >100
LC = Lethal Concentration
QA/QC
QA/QC summary Daphnia magna
Reference toxicant LC50 (95% CL) 5.5 (4.4 – 6.9) g/L NaCl1
Reference toxicant historical mean
(2 SD range) 6.2 (4.7 – 8.2) g/L NaCl
Reference toxicant CV 14%
Organism health history Acceptable
Protocol deviations None
Water quality range deviations None
Control performance Acceptable
Test performance Valid
1 Test date: May 06, 2020, LC = Lethal Concentration, SD = Standard Deviation, CL = Confidence Limits,
CV = Coefficient of Variation
WO#200878 Nautilus Environmental Company Inc. 2
Report By: Reviewed By:
Marcus Lee-Fraizer, B.Sc. Edmund Canaria, R.P. Bio.
Laboratory Biologist Senior Analyst
This report has been prepared by Nautilus Environmental Company Inc. based on data and/or samples
provided by our client and the results of this study are for their sole benefit. Any reliance on the data by
a third party is at the sole and exclusive risk of that party. The results presented here relate only to the
samples tested.
APPENDIX A – Summary of test conditions
Table 1. Summary of test conditions: 48-h Daphnia magna LC50 test.
Test species Daphnia magna
Organism source In-house culture
Organism age <24-hour old neonates
Test type Static
Test duration 48 hours
Test vessel 250-mL glass beaker
Test volume 200 mL
Test solution depth 6 cm
Test concentrations Five concentrations, plus laboratory control
Test replicates 1 per treatment
Number of organisms 10 per replicate
Control/dilution water Moderately-hard reconstituted water + 2.5 µg/L Se
Test solution renewal None
Test temperature 20 ± 2°C
Feeding None
Light intensity 400 to 800 lux
Photoperiod 16 hours light / 8 hours dark
Aeration None
Test measurements
Temperature, dissolved oxygen and pH measured daily;
salinity, hardness and alkalinity measured in the undiluted
sample at test initiation; conductivity measured at test
initiation and termination; survival checked daily
Test protocol Environment Canada (2000), EPS 1/RM/14, with 2016
amendments
Statistical software CETIS Version 1.9.4
Test endpoint Survival (48-hour LC50)
Test acceptability criterion for controls Survival ≥90%
Reference toxicant Sodium chloride (NaCl)
APPENDIX B – Toxicity test data
Daphnia magna Summary Sheet
Client:
Work Order No.:
~-,;/,:_ /"fz//1// Start Date!Time: jif~ f>/Z.,-z.o€_
£,oo g CT ¥....-.&..oz;~ Test Species: ~phnia magna
Set up by: ty,/1{,(
LflYI._
Sample Information: Test Validity Criteria:
Sample ID: ::?: 90% mean control survival and/or mobility and S2
# 'Zt'o - 6-~h ·- / 7.1/<•r/;z.,-,..., daphnids exibit immobility and/or mortality in any
Sample Date:
Date Received:
~ /Z /?-.9 z 0 single control replicate.
Sample Volume:
//1'"1 /J %u-....,, WQ Ranges:
z_ -I'.'. / L T ("C) = 20 ± 2; DO (mg/L) = 3.6 to 9.4; pH = 6 to 8.5
Test Organism Information:
Brood stock No.: b<./zq2oA:t8"'C.. Age of young (Day 0): <24 h
Avg No. young per brood in previous 7 d: /4 Mortality(%) in previous 7 d: e,
Days to first brood: 8
NaCl Reference Toxicant Results:
Reference Toxicant ID: /)~~ ..>'0 Stock Solution ID: 2<:>A/~.() '-Date Initiated:
48-h LCSO (95% CL): 41,, tJ 4 /k>Zc<>
S-: > L 1/-" Y ;_ ,',. '1 ) g/LNaCL
Reference Toxicant Mean and Historical Range: {,. Z. (¥- 7- - z. '2.) g/L NaCL
Reference T oxicant CV (% ):
Test Results: -Z:L 7!',!.,( {LSD cl q-z-,./4.,,__<,__,/ Z,, ..6..:... >/Pc Z.(v/4-)
Reviewed by: ~ Date reviewed: ~ ~I ;;:>bJ.i)
Version 1.5; Issued Sep. 30, 2015 Nautilus Environmental Company !nc.
Freshwater Acute
Client:
Sample ID:
Work Order No.:
48 Hour Toxicity Test Data Sheet
.&, 4-.. p <- B-f ik,: II /4...r.,_-=~ Start Date/Time: L I ~/z, ~ <2 N2.s L #-z..gc; -G.,,-.,k ·• -/.;..z../'Zc,u, CER#:_~-'"'--------
,z,,po 8:r:• 12. '.,,,. No. Organisms/volume:-'1-"0/c:2cc.0"'0m=L ______ _
,..,,_ - • TestOrganism:"O~.m=ag"'n~a-______ _
Setupby: /,(t,t,£:
Thermometer. CfJ!.ll:,;: pH meter/probe: ..}Q_1_J;z_ DO meter/probe: _.f:__l_j:2_ Cond.lSalinity nieterlprobe: .Jz.J_f,_
Concentration
%,(v/v)
Numberof I No. I I I I I Live Organisms Immobilized
1 Rep I ,
Temperature
('C) Dissolved oxygen
(mg/L)
pH Conductivity
(µSiem)
24 I 48 48 o I 24 I 48 I o I 24 I 48 I o I 24 I 48 I o I 48
/'-.,-t,,., / A I tQlio ,D i<i..olJti:o I 1-,:-> I fH'l~i}f,}]Jt. / jg 61 J:,.( I SS"t> I "?S'Y B
C
D
L ~- ·z 'S" A 11.o I lo 0 1q.ojf'1.cl1q,ol g".c,j',Z-xJ@<i~o!J~"!lS:( l</<eoJ "t'fvl
B
C
D
11,,JS" A II{) L10 -~ - 0 fw>_V.f. o !r_i;zi}_[j:,]:? 1 l J:.-;;J-11-'l ITfll .i:. 0 I s-z."l I en 1
B Cl-'
C
D
7%' A Ito l/o D ~-s-Jt1.0~'7P !1S.'fl'{fj;i.:Q1,1-Ti:Z !Jc-°! 700 \ ·rr-i-B
C
D
g'"O A 1/0 110 D rnsiN.olt"l,"1'15-"l.lkbl i..:.f \?.b!7.l I ""-"'1 lio~"l 11.,,,4 ,
B
C
D
J,00 A 1TITT10 C> fl~,o ilti .d 1"JJl\·p0 I 4:.l,! \$"\:.I 1-~ 1, b 11-ff'll 11t. I 1:J1 r B
C
D
Technician Initials I ciikl l,;tlv I (1""- I~ 111/Jf, ju~ /lWla>tl~I:11/?Jli>Yl,,w--l&~ rl.i,..,,..
Concentl]lt!on
Control (MH1""
Highest q_onc.
Hardness adjusted
Comments:
Hardness ... Alkalinitv*
*fll}_g_/_~ as CaCo3l
,; re I ::1-x .... /~o '2 "7--<:,
Sample Description: ~/4-d ,d-L /4:,.,.-: 7
Batch#: t'Ji-/Z '/ /,t,flHjJ,{(,7 .d previous# young/brood: / C,
Reviewed by: @:
Version 1.9: Issued July 19, 2017
-Act·astment- -A:-crusre
"-0 -/
./ //
1\-,.,r Mortality: Heartbeat checked _under microscope~ 'i:.t.
.., /44-1:.J , 4/4-,., v/4,,..,..- , f'PA-,_ ~-;?/u./4.-w ~ t/7/ ; 7
Previous 7~d Mortality{%): 0 Day of 1st Brood: __ 'g=---
Date reviewed: ~ 2-D ?1)Y!
Nautilus Environmental Company Inc,
APPENDIX C – Chain-of-custody form
G ~1H!!~N~~ Report to:
Company Mackenzie Pulp Mill Corporation
Address PO Box 2609, 1000 Coquiwaldi Rd.
City/State/Zip Mackenzie, BC, V0J 2C0
Contact Randeep Sandhu
Phone 250-997-2444
Email MPMC-Environment@mackenziepu!p.com
Sample Collection By: KV/CA ··'•'···•·•·•·•••.••· ·····•·••;,•.•,••.·.••w.•; .• ,,•;,•,•••••••'•,w, ·•.•;,•,••··.w...-,•··•···• • . ·.•••···w··•··••·•·•w.-;, ., •••. w,w .••. , .......... , •.
SAMPLE ID DATE TIME MATRIX
1 #2B0 - GRAB - 12/05/2020 12-May-20 7:30 water
2 2
I
4 4
;
6
7
8
9
10
'
' SPECIAL INSTRUCTIONS/COMMENTS (CLIENT}
Sample confirmation reciept, mortality update and report to required:
RELINQUISHED BY (CLIENT)
i,,io<Nam,) Cle\\\'\'_ -~)he.++ ~;I-.~ ~4/{A) , . -n !um)
Cornr,anv\ /v\oc.v\ C /l/lP (Date/Time'
TESTING LOCATION (Please Circle)
Burnaby 8664
Commerce Court Burnaby, British
Columbia, Canada VSA 4N7
Phone 604.420.8773
Invoice To:
Company Mackenzie Pulp Mill Corporation
Address PO Box 2609, 1000 Coquiwa!di Rd.
City/State/Zip Mackenzie, BC, V0J 2C0
Contact Kim Hornett
Phone 250-997-2473
Email [email protected]
PO No. 43011427
Sample Type: Grab X OR Composite O ,.w,w,•., .w,w.•,·.w,•,•;,•;,•;. N,,•,•.,v .• ·,.w.w.•;,•. ,•,•N,W,N,' ;,w, .•.w .•. , .• •,•••'-'•'•'•·•·••;,w,w, .~ .•.•,•,•••·••v;.•.; .• ·• ,w.
# OF CONTAINERS ANO
VOLUME (e.g. l x 20 L) COMMENTS
2x1L 20011g
·. SAMPLE RECEIPT l>ETAILS(LABORAT.ORY) i • : .• ..
1. TOtiil NO; o·t ·• 4.'·ke Present ~ioi i col'll:'illners· •. 2-- . - ... 1nt·ooif!r? :,: . ··.· •-, "• ·· ..
·.· ·.· .• .
i)··. 2. C(IUtler /.:otJ,..,,_,.r 5; Sfll'il .Pre·serit <
. •· i ·.· ~~ ,COO_l~r,(' \:.:
.·· .. ,,', .-•, : ~.::_J.~lt,la!~::._·,.'.·/
~--·•··•···
3. ·90,0:d. COndltl_On?_ {JIN.\ r.r.~_se~t-.~,~:{/' :•,,''.
,-. __ . ·-.-.· S~al?.>· .-· ·,<;·.. · ·
. • _i • .. ·. RECEIVED .BY. (LABORATORY) . ._· .. ·. ·.
1~ ·. / >y ·. •·• .. ··••. ·t11 .· / ·· ..
- . ·. · ·1Si~nii1UreJ \·.:_'.
·.
(Printed Name) : .. ..
.· . ··· .
r1~7 I 3/:JJJ ;1c:io ),1.,v$(/4,J" l1cornllii11,;1 · ·. ·._ : ,.'1Dateand!ime1 ;,, -· ·:,
Additional costs may be 'required for sample. disposal or storage. Payment net 30 unless otherwise contracted.
Chain of Custody
Date ___ p -ANALYSES REOUIRED
.
= 0
~ '-e
C = m
~ f· -9,
"" w
C 0. C m E ·2 a.
0 w - ~ "' ~ "' V
~ C ~
~ ·2 1 m 0 ro w s C
"' C
~ 0, cl "' C il! m ro C w
E I- E !- ;s ci "' ci "' ro
"' "' 0 ~ ~ ~ ~ "" 00 J, 00 J, "' st "" st "' 0:
X X s. l .
..
SAMPLE DESC_RIPTION AND COMMENTS (IABORATORY) .
· .. .. . . . .
··•·•·
I> ..
I . .
.
· ..
.
END OF REPORT
8664 Commerce Court, Burnaby, BC V5A 4N7
Acute Toxicity Test Results
Sample #2BO – GRAB – 19/05/2020,
collected May 19, 2020
Final Report
June 3, 2020
Submitted to: Mackenzie Pulp Mill Corporation
Mackenzie, BC
WO#200922 Nautilus Environmental Company Inc. 1
SAMPLE INFORMATION
Sample ID
Dates Receipt
temperature Collected Received Daphnia magna
test initiation
#2BO – GRAB –
19/05/2020
19-May-20 at
0820h
20-May-20 at
1125h
20-May-20 at
1440h 8.9oC
TEST
• Daphnia magna 48-h LC50 test
RESULTS
Toxicity test results
Sample ID LC50 (% v/v)
#2BO – GRAB – 19/05/2020 >100
LC = Lethal Concentration
QA/QC
QA/QC summary Daphnia magna
Reference toxicant LC50 (95% CL) 5.5 (4.4 – 6.9) g/L NaCl1
Reference toxicant historical mean
(2 SD range) 6.2 (4.7 – 8.2) g/L NaCl
Reference toxicant CV 14%
Organism health history Acceptable
Protocol deviations None
Water quality range deviations None
Control performance Acceptable
Test performance Valid
1 Test date: May 06, 2020, LC = Lethal Concentration, SD = Standard Deviation, CL = Confidence Limits,
CV = Coefficient of Variation
WO#200922 Nautilus Environmental Company Inc. 2
Report By: Reviewed By:
Marcus Lee-Fraizer, B.Sc. Edmund Canaria, R.P. Bio.
Laboratory Biologist Senior Analyst
This report has been prepared by Nautilus Environmental Company Inc. based on data and/or samples
provided by our client and the results of this study are for their sole benefit. Any reliance on the data by
a third party is at the sole and exclusive risk of that party. The results presented here relate only to the
samples tested.
APPENDIX A – Summary of test conditions
Table 1. Summary of test conditions: 48-h Daphnia magna LC50 test.
Test species Daphnia magna
Organism source In-house culture
Organism age <24-hour old neonates
Test type Static
Test duration 48 hours
Test vessel 250-mL glass beaker
Test volume 200 mL
Test solution depth 6 cm
Test concentrations Five concentrations, plus laboratory control
Test replicates 1 per treatment
Number of organisms 10 per replicate
Control/dilution water Moderately-hard reconstituted water + 2.5 µg/L Se
Test solution renewal None
Test temperature 20 ± 2°C
Feeding None
Light intensity 400 to 800 lux
Photoperiod 16 hours light / 8 hours dark
Aeration None
Test measurements
Temperature, dissolved oxygen and pH measured daily;
salinity, hardness and alkalinity measured in the undiluted
sample at test initiation; conductivity measured at test
initiation and termination; survival checked daily
Test protocol Environment Canada (2000), EPS 1/RM/14, with 2016
amendments
Statistical software CETIS Version 1.9.4
Test endpoint Survival (48-hour LC50)
Test acceptability criterion for controls Survival ≥90%
Reference toxicant Sodium chloride (NaCl)
APPENDIX B – Toxicity test data
Daphnia magna Summary Sheet
Client:
Work Order No.: #{...Ju.,_,:.,'.,__ A{e&'I( Start DatefTime ~
4 "e.o {:"':k, @/¥~:(__
'c-<:>o f 'Z-~ ?q?<>-.;..~ Test Species: Oa hnia ma na
Set up by: /4.t-F- / cc ;,
Sample Information: Test Validity Criteria:
Sample ID: :::: 90% mean control survival and/or mobility and :S2
-If ZS @-Cv..J, -11/4,s/z.;,., daphnids exibit immobility and/or mortality in any
,Ill "'7 /'f/z.o -u,, r single control replicate. Sample Date:
Date Received:
Sample Volume:
tf1J,, z,:, /-z., 2z WQ Ranges:
7 Z..Jt! / L T (°C) = 20 ± 2; DO (mg/L) = 3.6 to 9.4; pH = 6 to 8.5
Test Organism Information:
Broodstock No.: o S-of, 7.Diif: -t B Age of young (Day 0): <24 h
Avg No. young per brood in previous 7 d: { Mortality(%) in previous 7 d: 0
Days to first brood:
NaCl Reference Toxicant Results:
Reference Toxicant ID: /),.,_ pc.5"'0 Stock Solution ID: -z..,o N A-02
Date Initiated: _AY,..__ o 6 /-u,"'7.a 48-h LCSO (95% Cl): r.0y. - '-· "l) g/LNaCL
Reference Toxicant Mean and Historical Range: i. Z ( <,t. J - 'i;,z..} g/L NaCL
Reference Toxicant CV(%): ____ ,::./L-~· ________ _
Test Results: ~ ~.,g d. Cc.so ?IS -<At'f;,_,-4.,.,t ~ 4L :::.. 4a ~ &tv-1
Reviewed by: ~ Date reviewed: ~ -;,4 r ~
Version 1.5; Issued Sep. 30, 2015 Nautilus Environmental Company Inc.
Freshwater Acute
Client:
Sample ID:
Work Order No.:
48 Hour Toxicity Test Data Sheet
/4.a,.../u., ~ ef;,f. /;1/1/ /4,,-,o"'"---Z-~ Start Date!Time: ~3/ 1c>/=a. eJtYi{ £f Z8r;;-&w;,J, - 1£/o•;;/-z.p7,,:, CER#: __ <----------
1,oo q c. 'L ~ No. Organisms/volume: ---'1"'0"/2,_,0-=0"-m"'L'--------Test Organism: _,D'-'.m=a""gnccac....,. _______ _
Set up by: ~ /C c5
Thermometer: c.f.l!.< pH meter/probe: ~_!;;;_ DO meter/probe: __f_;___i2_ Cond./Salinity ~eter/probe: ___f?_1_f,_
Concentration Number of No.
Live Organisms Immobilized
%(vlv) Rep
24 48 48
/ ·--.-i,,,, I A /,D {D D
B
C
D
?.·z~ A IQ ID p
B
C
D
11.,.E A tO /0 D
B
C
D
-z_,'5"' A to /O 0
B
C
D
<;'O A ,~ /0 D
B
C
D
,1,...0 A i,o /o 0
B
C
D
Technician Initials /ffi,/,{ \\...\,'.}·~ \\\v...__
Hardness* Alkalinitv*
Concentration *(me/Las CaCo31
Control (MH\M '16 ...,_/
HiAhest cone. S?o ~z... ~ r"'I
Hardness adiusted
Comments:
Temperature Dissolved oxygen pH Conductivity
0
.. ~·.,.z::,
ifi.-c-
lt/.0
;q.o
lti,o
it/,O
ITT{
('C) (mg/L) (µSiem)
24 48 0 24 48 0 24 48 0 48
1a.r l'iP &.-4 '?,,'5' JA, q.1. 'il."J f-1 s'i""i .~s-i.-
/f.-', l"iP <i.'?, <bz.. J-, i/ g~ .v 'i( .0 J:.. <> az_g I tr,"'
11-" (qP t-:+ <LI C. ; :~'I R.c J.._ D '.>c<> -,::,<
lfi:, /t;p g.1- IJ..l, J-,'j) 7--~ 'J,'l I-" 61,,3 /"(, I
·;;.,; i"l, ,0 'l..'-f L 1 b :l I '7'. "'.l.. ,,'\ ~9 q,~ q·i,D
/ti-'> /'/? 7-,3 I-.;. 1o S',5 17.$' 7. l, i.s- I/be-I /~oJr
duf \\_<_"'- r,;:;;-,.,- ,111.1-r,, ,,\»..._ lh1,u:-- t'l,M,f 'v,..'·' ,//~./ 1,;,,,_~
mmai ustmem ustea
Temp ('Cl /'i.o /
DO (moil) -;7. ~ V
pH '1--"> /
Cond fuS/cm I(., of ./
Sa!initv (""n b, e £
~rr Mortality: Heartbeat checked under microscope r"1 'cL
Sample Description: -/&,bld ~ ~ ' J '= ~?• rd 1 '21,.f'J < 7 0 d,.,,,,__, < -
I f&i..,. ~:k'.44:1'., \
" ~a Batch#: f\ ')Cb ·ti;, A-tK 7--d previous # young/brood: { -::J: Previous 7~d Mortality{%): 0 Day of 1st Brood.: __ ..,!,_ __
Reviewed by: ~ Date reviewed: rtYu.1 :;,4 r d1J,;,o
Version 1.9; Issued July 19, 2017 Naumus Environmental Company lnc.
·-
APPENDIX C – Chain-of-custody form
G ~~H!!~N~~ Report to:
Company
Address
City/State/Zip
Contact
Phone
Sample CoHl!~~!~-~ By: ~'..l..
SAMPLE ID
11#280 - GRAS -19/05/2020
Mackenzie Pulp Mill Corporation
PO Box 2609, l0~Q Coquiwaldi Rd.
Mackenzie, BC, V0J 2CO
Randee_e. Sandhu
250-997-2444
MPMC-Environment@mackenziepu!p.com
M PMC-T echn [email protected] m
KV
DATE TIME MATRIX
19-May-20 8:20 water
TESTING LOCATION (Please Circle}
Burnaby 8664
Commerce Court Burnaby, British
Columbia, Canada VSA 4N7
Phone 604.420.8773
lnvoice To:
Company
Address
Mackenzie Pulp Mill Corporation
PO Box 2609, 1000 Co.9.uiwaldi Rd.
City/State/Zip Mackenzie, BC, V0J 2C0
Kim Hornett
250-997-2473
Contact
Phone
PO No.
43011427
Sample Type: Grab X OR
# OF CONTAINERS AND
VOLUME (e.g. 1 x 20 L)
2 x 1 L
Composite 0
COMMENTS
2..00 qzz
Chain of Custody
Date ___ Page_of.
ANALYSES REQUIRED
I E e
a = = m
( ~ .9, cr, K .s a
m
! C .9, 0 © ~ ~ 0,
'.:J u C
l a ·a m 0 m © f C ~ C ~ 0, u cr, u m ~ m a w
E I- E I- J!l ci "' ci "' m
"' "' 0 .c .c .c .c ~
$ "' $ "' ~
O'> O'> "' .·
X X K,9
21-------1----+--+---+-------+----------+-t-+-t-+--+-l-+-+-t--t--
:1 I I I I I I I I I I I I I I I I --
51--------1-----+---+---+-------+-----------+--t--t--ir--t--i---i--i---i--t--;--c--
:1 I I I I I I I I I I I I I I I I --
-8 I I I I
' . -9 I I J I
10 I I 7 7 .· . I · -- -- - -- - I ' SAMPLERECEll'Tl>ETAILS(u\li0.111\TORY) SAMPLE llESCRIPTIONAf:ID COMMENTS (LABOffAT(!RYf ' SPECIAL INSTRUCTIONS/COMMENTS (CUENT)
-- -
Sample confirmation reciept, mortality update and report to required:
REUNQUISHED BY (CLIENT)
1, ·Ttital Nc;i;··:of
KVI c;..1J,S, GI Va 1Ac~a>-t.A 7''1 (Prir>t Name =-----F'"'"''"l£!!•d1:!N•,cm,i_e)_c_'--c...,---"---"---"-'-,,.-----"---"Q;,,L!!!!eL.:......:......:........:,
Y\ cte,O,Jl<Ad4> ftt.Lf- tvw 14 ).lo@_; I\ '.o /l1'i;&'"( /.. /y!t,j 2f:/13l //,7-c _Company) 'tfoate/l"ime) (~ompanyl :· · ·· · · · . '," (D~feMdTlll\_e)
Additional costs may be required for sample disposal or storage. Payment net 30 unless othenvise contracted.
END OF REPORT
8664 Commerce Court, Burnaby, BC V5A 4N7
Acute Toxicity Test Results
Sample #2BO – GRAB – 26/05/2020,
collected May 26, 2020
Final Report
June 10, 2020
Submitted to: Mackenzie Pulp Mill Corporation
Mackenzie, BC
WO#200983 Nautilus Environmental Company Inc. 1
SAMPLE INFORMATION
Sample ID
Dates Receipt
temperature Collected Received Daphnia magna
test initiation
#2BO – GRAB –
26/05/2020
26-May-20 at
0730h
27-May-20 at
1200h
27-May-20 at
1350h 7.8oC
TEST
• Daphnia magna 48-h LC50 test
RESULTS
Toxicity test results
Sample ID LC50 (% v/v)
#2BO – GRAB – 26/05/2020 >100
LC = Lethal Concentration
QA/QC
QA/QC summary Daphnia magna
Reference toxicant LC50 (95% CL) 6.8 (5.7 – 8.1) g/L NaCl1
Reference toxicant historical mean
(2 SD range) 6.2 (4.7 – 8.3) g/L NaCl
Reference toxicant CV 14%
Organism health history Acceptable
Protocol deviations None
Water quality range deviations None
Control performance Acceptable
Test performance Valid
1 Test date: May 27, 2020, LC = Lethal Concentration, SD = Standard Deviation, CL = Confidence Limits,
CV = Coefficient of Variation
WO#200983 Nautilus Environmental Company Inc. 2
Report By: Reviewed By:
Marcus Lee-Fraizer, B.Sc. Andy Diewald, B. Sc.
Laboratory Biologist Senior Analyst
This report has been prepared by Nautilus Environmental Company Inc. based on data and/or samples
provided by our client and the results of this study are for their sole benefit. Any reliance on the data by
a third party is at the sole and exclusive risk of that party. The results presented here relate only to the
samples tested.
APPENDIX A – Summary of test conditions
Table 1. Summary of test conditions: 48-h Daphnia magna LC50 test.
Test species Daphnia magna
Organism source In-house culture
Organism age <24-hour old neonates
Test type Static
Test duration 48 hours
Test vessel 250-mL glass beaker
Test volume 200 mL
Test solution depth 6 cm
Test concentrations Five concentrations, plus laboratory control
Test replicates 1 per treatment
Number of organisms 10 per replicate
Control/dilution water Moderately-hard reconstituted water + 2.5 µg/L Se
Test solution renewal None
Test temperature 20 ± 2°C
Feeding None
Light intensity 400 to 800 lux
Photoperiod 16 hours light / 8 hours dark
Aeration None
Test measurements
Temperature, dissolved oxygen and pH measured daily; salinity,
hardness and alkalinity measured in the undiluted sample at
test initiation; conductivity measured at test initiation and
termination; survival checked daily
Test protocol Environment Canada (2000), EPS 1/RM/14, with 2016
amendments
Statistical software CETIS Version 1.9.4
Test endpoint Survival (48-hour LC50)
Test acceptability criterion for controls Survival ≥90%
Reference toxicant Sodium chloride (NaCl)
APPENDIX B – Toxicity test data
Daphnia magna Summary Sheet
Client:
Work Order No.:
M,ullfmie £ult, Mill rn rpc.wtin YI 2C09Z"-
Sample Information:
Sample ID:
Sample Date:
Date Received:
Sample Volume:
'-1\2.f:,i\- 0gp.,p, - 26/0'5/2020 Ho,lt ?0 /202£)
Hc1v 21 12020 'lX IL
Test Organism Information:
Start Datemme: Muy J.7 /20:2Q & ,3:.50 k Test Species: Daphnia magna
Set up by: ML.F / cu;
Test Validity Criteria:
~ 90% mean control survival and/or mobility and :52
daphnids exibit immobility and/or mortality in any
single control replicate.
WQRanges:
T (°C) = 20 ± 2; DO (mg/L) = 3.6 to 9.4; pH = 6 to 8.5
Broodstock No.:
Age of young (Day 0):
01v2.q2 o A~ei <24 h
Avg No. young per brood in previous 7 d: 16 Mortality(%) in previous 7 d:
Days to first brood: 2.
NaCl Reference Toxicant Results:
Reference Toxicant ID:
Stock Solution ID:
Date Initiated:
48-h LC50 (95% CL):
i)n1DC'51 '.2flNu.n'2
~'°'" ?J now . "' '" . ) /,,. ,. I '1.7- 1.. l _gjLNaCL
Reference Toxicant Mean and Historical Range: fu ·2. (4.1 -i. 3) _gjl NaCL
Reference Toxicant CV(%): ___ ,_I-'-----------
Test Results: -n,,,,.,. !4-Xhr L.t i:=.;o ~-~ ect1n111tPd tr, \-iP 1100-':/u i.vlv).
Reviewed by: Date reviewed: ~ 1/2,:::>
Version 1.5; !ssued Sep. 30, 2015 Nautilus Environmental Company Inc.
Freshwater Acute
48 Hour Toxicity Test Data Sheet
Client:
Sample ID:
Work Order No.:
//fM./r-_-r.,1,,_ fl.f Jt'/1 /4,,.,,,-..¢;,.._ Start Date/Time: /11_47 Zrz:'"-i,,;:,,.@ /SsoL. :ii 7,g,:, -6.....,_t, ·- U / o, S: /1,,;,.,_,.,, CER #: __ > _________ _
r . ·"7,.pe q:;,,; No. Organisms/volume: ~1~0/-=2~00~m~L~-------
Test Organism:-=O"".m=ag"-n','a-,,---,-------Set up by: /1#'i: / cc. ~
Thermometer. ('.f fi1"<:"pH meter/probe: _i_; b DO meter/probe: _!i__t~ Cond./Salinity meter/probe: _!::__}~
Concentration Number of No.
Temperature Dissolved oxygen pH Conductivity Live Organisms
Immobilized ('C) (mg/L) (µSiem)
/.,{vi'-') Rep
24 48 48 0 24 48 0 24 48 0 24 48 0 48
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Temn /"C\ 1q.o ,
DO /mo/L\ l' .o /
Control MH lnH 7,5 .,/
Hi hest cone. Cond (uS/cm) 14q2_ /
Hardness ad·usted Salinitv loot\ C ;-1 /.
Comments: Mortality: Heartbeat checked under microscope fi; ~ Req_ owed
w
Sample Description: i:i1vr,,d, , bYGNU pu.i P'J odr,,r s~ Batch#: l:Ji./2/11,;;> i'\'t'~ 7-d previous# young/brood:
I C.l i
-Z.-0 Previous 7-d Mortality(%):
pi y t'i ( l1 \c.l,Jei
~ Day of 1st Brood:.~$~--
Reviewed by: er Date reviewed: &v-t.- YD Version 1.9; Issued July 19, 2017 Nautilus Environmental Company Inc.
APPENDIX C – Chain-of-custody form
11.-'1""'-' L.U"-N1•v111 \.-n::a;,"' .,.., .. ,..,,
Chain of Custody
G ~~H!!h~~ Burnaby 8664
Commerce Court Burnaby, British
Columbia, Canada VSA 4N7
Phone 604.420.8773 Date ___ Page_of
ANAL VSES REnUIRED
Report to: Invoice To:
Company Mackenzie Pulp Mill Corporation Company Mackenzie Pulp Mill Corporation I
Address PO Box 2609, 1000 Coquiwafdi Rd. Address PO Box 2609, 1000 Coquiwaldi Rd. e City/State/Zip Mackenzie, BC, V0J 2C0 City/State/Zip Mackenzie, BC, VOJ 2C0 :t e • ~ • = Contact Randeep Sandhu Contact Kim Harnett
ro
~ e E,
Phone 250-997-2444 Phone 250-997-2473 cr, 8. C • ·a ro ! '
Email [email protected] Email [email protected] 0 • E, v> ~ cr,
[email protected] PO No. ':l u C ~ 43011427 • ·a
ro 0 ro • s ] C v> C
~ Sample Collection By: KV Sample Type: Grab X OR Composite 0 cr, ':l
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# OF CONTAINERS AND "' "' 0
SAMPLE ID DATE TIME MATRIX COMMENTS .c .c .c .c v>
VOLUME {e.g. 1 x 20 L) J, "' J, "' v>
st m st m i2
l #280 - GRAB - 26/05/2020 26-May-20 7:30 water 2 X 1 L I IV If 2 0011 o 3 X X 28
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SPECIAL INSTRUCTIONS/COMMENTS (CUENT) ..
SAMPLE RECEIPT DETAILS_ (t.<(BORATORY) . - .. > SAMPLE .DESCRIPTION AN.D,COMMENTS (LJ\BORATORY) .• ...
1; _TOtat_.N?·:•:0!•:::\:_::: :-:"i· .. -· I k' ~·:.: ~~,: P~~-e-~_t._ •·• @ · .. i .i . . \ ·. . . . .• . .,: ,• :.··· · ... ·· >
Sample confirmation reciept, mortality update and report to required: Containers-.-:,•.-. ·· ,.i. ln:cciorer? / ... :,•/~\<•··
{~ourlor •:··•· i. · :.•• U:Of.iJ fs<,IP,eserit v1rp) _ ... :- .· :-: ·.. /.-· .... •.-.·· I:•:·:·.. .• . .-,. '· :-,.--·. c;mCooler?· .. ·--> ........ · .; .:" _:..-· . ·•· .· .
[email protected] . i·· .. . • .. •.·.• ··.·· .. ···. •,..;,.( 6,.lrif\1"5 .. _·.'.i •i> Yi~ 3 .. ~ot,_d.,C.~_11_d,l!i,~~! :.· . ..- ·~~-~-~'!.~t:~.n :".'-._:-'.:. '·· _. . \ -.,. ' ::' · ·:·.-. ··.·.: :;•:: ·. ··: . -'<·:'.:"/;/.·:.:·i• Si!al?:.:,: .:::< ,_.:;•··_-.: ,:- , _ :: .. ·•· ..
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. .··.• RECEIVED IIY (LABORAT<lRY) • • RELINQUISHED BY (CLIENT) . · •:·: ... ·, ' ',-: . ,."' '" .
~_/£ ··.•·.·•·•·.···~·-··••·i. \
.i·: i....... 1:1-1 ..•..... • \> p,;o,N,m,,C lc,:,re. AhtioH- le 'Jir}
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Comnanv) · .. •.:, "_:· .. ··ioa1~::Z·Timei ·· · :.:· . .
Additional costs may be required for sample disposal or storage. Payment net 30 unless otherwise contracted.
END OF REPORT
Precision Accuracy BlanksDate Test Results Average % Deviation Acceptance Date Std. Value Test % Deviation Acceptance Date Test Results Acceptance
from Average Criteria Result from Standard Criteria mg/l Criteria
A) Non-Filterable Residue (TSS)
05-May-20 87.90 88.5 0.6 15.0 < 2.0 mg/l
89.05 05-May-20 -3.05
12-May-20 90.20 86.5 4.3 12-May-20 -1.85
82.80 19-May-20 -3.90
19-May-20 91.00 91.4 0.4 26-May-20 -2.60
91.80
26-May-20 66.70 61.6 8.2
56.55
*Note: extremely low concentrations represent greater deviation possibilities
B) pH
06-May-20 7.01 7.01 0.1 15.0 Not applicable for pH test.7.00
12-May-20 7.03 7.04 0.1
7.05
19-May-20 7.12 7.16 0.5
7.20
26-May-20 7.69 7.78 1.1
7.86
C) Nutrients (TESTING DONE BY ALS Environmental)
D) BOD (TESTING DONE BY ALS Environmental)
E) AOX (TESTING DONE BY ECONOTECH SERVICES LTD.)
See attached Certificate of Accreditation and Final Scope of Testing
documentation for Econotech Services Ltd & ALS Environmental issued
by the Canadian Association for Laboratory Accreditation Inc. (CALA).
Subsequent submissions will refer to this documentation.
See also last PT test done by Mackenzie Pulp Mill.
May 2020
Quality Assurance Data
SAMPLE ANALYTE UNITS ASSIGNED REPORTED S Z SCORE
STUDY DATE:
LABORATORY NAME:
March 2020 Study
Mackenzie Pulp Mill - Internal Compliance Lab
METHOD
DATE: PAGE:PROFICIENCY TESTING CANADA TESTING REPORT
3033
CODE
PARTICIPANT NO.:
MATRIX: Water (Inorganic)
APP
June 19, 2020
SCORE BIAS SUMMARYN ±U
3
SolidsTEST GROUP:
C04A-1 Total Suspended Solids mg/L 10 10 2GRAVIMETRIC 0033246 187 .1
C04A-2 Total Suspended Solids mg/L 46 45 5GRAVIMETRIC -.2033246 183 .4
C04A-3 Total Suspended Solids mg/L 95 84 10GRAVIMETRIC -1.1033246 186 .9
C04A-4 Total Suspended Solids mg/L 200 184 20GRAVIMETRIC Acceptable-.8033246 186 2 92
C04A-1 Volatile Suspended Solids mg/L 8.9 9.6 1.3GRAVIMETRIC .538050850 57 .2
C04A-2 Volatile Suspended Solids mg/L 34.9 35.2 4.4GRAVIMETRIC .068050850 59 .7
C04A-3 Volatile Suspended Solids mg/L 78.5 72.4 9.8GRAVIMETRIC -.622050850 59 2
C04A-4 Volatile Suspended Solids mg/L 162 158.1 20.2GRAVIMETRIC Acceptable-.193050850 58 3 95
BOD/CBODTEST GROUP:
C04B-1 BOD (5 day) mg/L 48 45 6D.O. METER -.5033247 103 .8
C04B-2 BOD (5 day) mg/L 83 82 10D.O. METER -.1033247 104 1
C04B-3 BOD (5 day) mg/L 167 130 23D.O. METER -1.609033247 105 3
C04B-4 BOD (5 day) mg/L 220 167 32D.O. METER Acceptable-1.656033247 104 4 86
CODTEST GROUP:
C04D-1 COD mg/L 76 80 5COLORIMETRIC .8044972 94 .7
C04D-2 COD mg/L 116 127 7COLORIMETRIC 1.571044972 96 .9
C04D-3 COD mg/L 309 315 13COLORIMETRIC .462044972 93 2
C04D-4 COD mg/L 485 513 18COLORIMETRIC H Acceptable1.556044972 95 2 84
pHTEST GROUP:
C15-1 pH pH units 5.1 5.1 .1pH METER 0033248 237 .008
C15-2 pH pH units 7.12 7.06 .1pH METER -.6033248 239 .008
C15-3 pH pH units 3.34 3.44 .1pH METER 1033248 238 .008
*DNS = Did Not Submit Results ± U = uncertainty of the Assigned Value
SAMPLE ANALYTE UNITS ASSIGNED REPORTED S Z SCORE
STUDY DATE:
LABORATORY NAME:
March 2020 Study
Mackenzie Pulp Mill - Internal Compliance Lab
METHOD
DATE: PAGE:PROFICIENCY TESTING CANADA TESTING REPORT
3033
CODE
PARTICIPANT NO.:
MATRIX: Water (Inorganic)
APP
June 19, 2020
SCORE BIAS SUMMARYN ±U
4
C15-4 pH pH units 8.25 8.22 .1pH METER Acceptable-.3033248 242 .008 93
*DNS = Did Not Submit Results ± U = uncertainty of the Assigned Value
This laboratory is accredited in accordance with the recognized International Standard ISO/IEC 17025:2017. This accreditation demonstrates technical competence for a defined scope and the operation of a laboratory quality management system (refer to joint ISO-ILAC-IAF Communiqué dated April 2017).
Nautilus Environmental Inc. 8664 Commerce Court Burnaby, British Columbia
Accreditation No.: A3525 Issued On: September 10, 2019 Accreditation Date: March 6, 2007 Expiry Date: March 10, 2022
This certificate is the property of the Canadian Association for Laboratory Accreditation Inc. and must be returned on request; reproduction must follow policy in place at date of issue. For the specific tests to which this accreditation applies, please refer to the laboratory’s scope of accreditation at www.cala.ca.
President & CEO