Warm-Up / EOC Prep1. A segment of a DNA strand has the following bases:

TAC GATWhat is the complementary strand of DNA?A. UAG CAU B. TAG CATC. ATG CTA D. AUG CUA

2. Which relationship is most similar to the relationship below?

tRNA : ribosomeA. book : publisher B. truck : factoryC. key : lock D. baker : pie

Agenda•Warm-up•Notes- Mutations•Mutation Practice WS•BINGO (If we have time)•DNA Quiz•Clean-up•Cool-Down

Mutations and Gene Regulation

•Remember: In Eukaryotes, DNA is found in the nucleus.

•For the most part, all of the cells in an organism’s body have the same DNA. However, we have learned in the past that cells are specialized for specific tasks and parts of the body.

•How can they be specialized but have the exact same DNA, the exact same instructions?

•Cell differentiation is the way that cells become different from each other as they go through mitosis.

•At first, all cells are the same and are not specialized. These cells are called stem cells.

•As they grow and divide they become differentiated and specialized into heart cells, brain cells, liver cells, etc.

•The differentiation of cells, despite the fact that all cells have the same DNA, occurs due to gene regulation. Both prokaryotic and eukaryotic cells can regulate which genes are expressed and which are not, depending on the cell’s needs.

Cell Organization Review•Specialized cells have specific jobs.•A group of similar cells working together to perform the same function is called a tissue.

•Ex: muscle, epithelial, connective, nervous

•A group of similar tissues working together to perform the same function is called an organ

•Ex: heart•A group of similar organs working together to perform the same function is called an organ system.

•Ex: circulatory system

•The four levels of organization:

1.Cell

•2. Tissue•Four types: muscle, connective, epithelial, nervous

•3. organ

•4. organ system

•As different cells respond to the environment they produce different types and amounts of proteins by “turning on” some genes and “turning off” other genes.

•Remember: A gene is a segment of DNA located on a chromosome that codes for a specific hereditary trait.

•This protein production is what specializes different cells for different jobs.

•Turning on and off genes can be an advantage or a disadvantage to the cell. Gene regulation can cause the overproduction or underproduction of proteins as well as the production of proteins at incorrect times.

•An overproduction of proteins can cause cancer (the uncontrolled growth of cells), however, overproduction of proteins can also help the body, such as in injury repair

•Just like poor gene regulation, mutations can also cause problems in cells.

•A mutation is a change in the nucleotide-base sequence of a gene or DNA molecule

•When the cell copies its DNA, the enzyme DNA polymerase is in charge of proofreading the new DNA strand for errors. If it makes an error and doesn’t correct it, a mutation occurs.

•Mutations can disrupt the functions of proteins.

Examples

•DNA: TAC-GGC-GAG-TAG-CCT•RNA: AUG-CCG-CUC-AUC-GGA•AA: Met - Pro -Leu -Iso -Gly

•These amino acids in this order make up a specific protein that is needed by the body.

Examples

•If one DNA nucleotide is changed, look what happens to the protein…

•DNA: TAC-GTC-GAG-TAG-CCT•RNA: AUG-CAG-CUC-AUC-GGA•AA: Met - Glu -Leu -Iso -Gly

•Now a point mutation has been made, or perhaps an amino acid sequence that is different and will do nothing.

Examples

•A different type of mutation can occur called a frame-shift mutation. What would happen if DNA Polymerase accidently removed one of the nucleotides in the original DNA sequence?

•DNA: TAC-GGC-GAG-TAG-CCT•DNA: TAC-GGC-AGT-AGC-CT•RNA: AUG-CCG-UCA-UCG-GA•AA: Met - Pro -Ser -Ser - ---

X

Types of Mutations•Gene Mutations▫Point Mutation (not serious usually)▫Frame Shift Mutations (serious)

Insertion Deletion

•Chromosome Mutations (all very serious)▫Deletion Insertion▫Inversion ▫Translocation

Types of Gene Mutations

Types of Chromosome Mutations

REVIEW

•What is a mutation?

•A change in a nucleotide base sequence

•Name that mutation: •THE CAT SAT THE TAC SAT

•Inversion

•Name that mutation:•THE CAT SAT THE CT SAT

•Deletion

•Name that mutation:•THE CAT SAT THE CATTA SAT

•Insertion

•Name that mutation:•THE CAT SAT ITH ECA TSA

•Frameshift mutation

Mutation Practice•If your birthday is..•Jan-Mar: insertion•Apr-June: deletion•July-Sept: inversion•Oct-Dec: point mutation

•Mutate this mRNA strand according to your birthday.

•ACU CGA ACA

Now..

•Take the original sequence and translate it.

•Also take your mutated sequence and translate it.

•Did the amino acid chain change because of the mutation?

Mutation Practice•If your birthday is..•Jan-Mar: point mutation•Apr-June: insertion•July-Sept: deletion•Oct-Dec: inversion

•Mutate this mRNA strand according to your birthday.

•CAC UAG AGU

Now..

•Take the original sequence and translate it.

•Also take your mutated sequence and translate it.

•Did the amino acid chain change because of the mutation?

Mutation Practice•If your birthday is..•Jan-Mar: inversion•Apr-June: point mutation•July-Sept: insertion•Oct-Dec: deletion

•Mutate this mRNA strand according to your birthday.

•UCC GAC GCA

Now..

•Take the original sequence and translate it.

•Also take your mutated sequence and translate it.

•Did the amino acid chain change because of the mutation?

Mutation Practice•If your birthday is..•Jan-Mar: deletion•Apr-June: inversion•July-Sept: point mutation•Oct-Dec: insertion

•Mutate this mRNA strand according to your birthday.

•ACT CGA UCG

Now..

•Take the original sequence and translate it.

•Also take your mutated sequence and translate it.

•Did the amino acid chain change because of the mutation?

•Whiteboards & Markers Away!

•Complete the Mutation Practice worksheet.

Complementary DNA Strand

•TCTAAC•CTTAAG•GAGATC•AGTCAG•CAGGTT•AACGUC•GCTACG•CGAATT

Transcribe DNA to mRNA

•ATCGAA•GGCATA•CTAGCA•TTCAGG•AGTCAT•GTCAGT•CCAGGT•AATTGC

Reverse Transcription: mRNA to DNA •AUGCAA•UGAACC•AUGCCA•GUCACU•ACUGUA•CGUCAC•UCGACC•ACGUUA

Translate: mRNA to protein chain•AUGCAA•UGAACC•AUGCCA•GUCACU•ACUGUA•CGUCAC•UCGACC•ACGUUA

Cool-Down1- What is the difference between transcription and translation (consider inputs and outputs)?

2- A mutation changes the original sequence of A-T-T-C-G-A to T-T-A-C-G-A. What kind of mutation was this? Did it shift the reading frame?

3- Fictional: in the battle between DNA and protein, who makes the stronger case for determining who you are as a person?

Mutation Practice

Name the correct mutation!•Normal Gene: ACA TCA CCG

•Mutation # 1: ACG TCA CCG•Mutation # 2: ACA ACT CCG•Mutation # 3: AAT CAC CG

Mutation Practice

Name the correct mutation!•Normal Gene: TCG CCG ACG

•Mutation # 1: TCT GCC GAC G•Mutation # 2: TCG CCG CCG ACG

•Mutation # 3: GCC GCT ACG