vol. 258, no. 3 biological chemistry · vol. 258, no. 3 the journal of february 10, 1983 biological...

Vol. 258, No. 3 February 10, 1983 The Journal of

Biological Chemistry Cop.yright 0 1983 by the American Society of Biological Chemists, Inc., 428 East Preston St., Baltimore, MCI. 21202 1J.S.A.

CONTENTS*

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COMMUNICATIONS

Sequential association of nucleolar 7-2 RNA with different autoantigens.

Carl Hashimoto and Joan A. Steitz

Detection of a nucleolar 7-2 ribonucleoprotein and a cytoplasmic 8-2 ribonucleoprotein with autoantibodies from patients with scleroderma.

Ram Reddy, Eng M. Tan, Dale Henning, Katsu Nohga, and Harris Busch

Messenger RNA for the 73,000-dalton poly(A)-binding protein occurs as translationally repressed mRNP in duck reticulocytes.

Kinsey Maundrell, Maria Tereza Imaizumi-Scherrer, E. Stuart Maxwell, Olivier Civelli, and Klaus Scherrer

Labeling of high affinity ATP binding sites on the 53,000- and 160,000-dalton glycoproteins of the sarco- ulasmic reticulum with the photaffinity probe 8-N3- [a-=P]ATP.

Kevin P. Campbell and David H. MacLennan

Localization of membrane proteins by the use of a photoreactive fatty acid incorporated in viva into ve- sicular stomatitis virus.

John Capone, Pierre Leblanc, Gerhard E. Gerber, and Hara P. Ghosh

Nucleotide sequences of two pea cDNA clones encoding the small subunit of ribulose 1,5-bisphosphate carboxylase and the major chlorophyll a/b-binding thylakoid polypeptide.

Gloria Coruzzi, Richard Broglie, Anthony Cashmore, and Nam-Hai Chua

Oligomeric structure and catalytic activity of G type Casein kinase. Isolation of the two subunits and rena- turation experiments.

Cleude Cachet and Edmond M. Chambaz

A novel thrombin-reactive protein complex in human platelets.

Mohanathasan Chelladurai, Nancy G. Fossett, and Pan- kaj Gang&y

Effects of insulin on phenylephrine-induced activation of phosphorylase and phosphatidylinositol turnover in isolated hepatocytes.

Andrew P. Thomas and John R. Williamson

Ribose. An oxidation product of glucose B-phosphate in microsomal fraction.

Yukinobu Hino and Shigeki Minakami

Opiate receptor binding affected by guanine nucleotide. Partial loss of sensitivity to guanosine-5’-(/3,y-imido) triphosphate evident in vitro.

.Jan S. Rosenbaum and Wolfgang SadCe

Ribonuclease D is not essential for the normal growth of Escherichia coli or bacteriophage T4 or for the biosynthesis of a T4 suppressor tRNA.

Richard T. Blouin, Richard Zaniewski, and Murray P. Deutscher

Stereospecific elimination of hydrogen at C-10 in ei- cosapentaenoic acid during the conversion to leuko- triene Cs.

Sven Hammarstrtim

Sequence determination for N-linked oligosaccharides through the use of the nuclear Overhauser effect.

Jean-Robert Brisson and Jeremy P. Carver

1435 Pertussis toxin inhibits enkephalin stimulation of GTPase of NGlOS-15 cells.

Drusilla L. Burns, Erik L. Hewlett, Joel Moss, and Mar- tha Vaughan

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Separation and identification of type 1 and type 2 protein phosphatases from rabbit reticulocyte lysates.

J. Gordon Foulkes, Vivian Ernst, and Daniel H. Levin

Phosphofructokinase mutants of yeast. Biochemistry and genetics.

Zita Lobo and Pabitra K. Maitra

The dynamics of formation of a collagen-phosphopho- ryn conjugate in relation to the passage of the miner- alization front in rat incisor dentin.

George D. Maier, Joseph H. Lechner, and Arthur Veis

The interaction in uitro of the intermediate filament ~z-2 vimentin with naturally occurring RNAs and

Peter Traub, W. James Nelson, Siegfried Kiihn, and Constantin E. Vorgias

GTP-dependent anion-sensitive adenylate cyclase in snail ganglia potentiation of neurotransmitter effects.

Philippe Deterre, Henri Gozlan, and Joel Bockaert

Measurement of the electrochemical proton gradient in submitochondrial particles.

Edwurd A. Berry and Peter C. Hinkle

Proteolytic conversion of arginine-vasopressin and oxytocin by brain synaptic membranes. Characterization of formed peptides and mechanisms of proteolysis.

J. Peter H. Burbach and Jos L. M. Lebouille

The action of insulin on hepatic fructose 2,6-bisphosphate metabolism.

Simon J. Pilkis, Ted D. Chrisman, M. Raufczt El-Magh- rabi, Anne Colosia, Eric Fox, Jo Pilkis, and Thomas H. Claus

Turnover of cytochrome c oxidase from Paracoeeas denitrificans.

Juergen K. V. Reichardt and Quentin H. Gibson

NADPH-dependent production of Oxy radicals by purified components of the rat liver mixed function oxidase system. I. Oxidation of hydroxyl radical scaveng- ing agents.

Gary W. Winston and Arthur I. Cederbaum

NADPH-dependent production of Oxy radicals by purified components of the rat liver mixed function oxi- ci;;;si[stern. II. Role in microsomal oxidation of


a-Adrenergic regulation of enzymes of amino acid metabolism in primary cultures of adult rat hepatocytes.

Chiseko Noda, Toshikazu Nakamura, and Akira Ichi- hara

Resolution and reassociation of three distinct components from pig heart phosphoprotein phosphatase.

Teiji Imaoka, Michinori Imazu, Hirofumi Usui, Nobu- hisa Kinohara, and Masao Takeda

Purification and characterization of DNase VIII. A 5’- 3’ directed exonuclease from human placental nuclei.

Antonia M. Pedrini and Lawrence Grossman

* The CONTENTS arranged by Subject Categories will be found immediately following these CONTENTS. Full Instructions to Authors will be found in THE JOURNAL, 258, 1 (1983), and reprints may be obtained from the editorial office.

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1544 “0 labeling of adenine nucleotide a-phosphoryls in platelets. Contribution by phosphodiesterase-catalyzed hydrolysis of CAMP.

Thomas P. Krick, and Nelson D. Goldberg Timothy F. Walseth, John E. Gander, Susan J . Eide,

1559 Mossbauer study of iron bleomycin and its activation

Richard M. Burger, Thomas A. Kent, Susan Band Hor-

1565 Thyrotropin-releasing hormone-diphtheria toxin-re- lated polypeptide conjugates. Potential role of the hydrophobic domain in toxin entry.

Patricia Bacha, John R. Murphy, and Seymour Reichlin

1571 Inhibition of electron transfer by 3-alkyl-2-hydroxy- l,4-naphthoquinones in the ubiquinol-cytochrome c oxidoreductases of Rhodopseudomonas sphaemides

quinone-binding site and the Rieske iron-sulfur clus- and mammalian mitochondria. Interaction with a ubi-

ter. Katsumi Matsuura, John R. Bowyer, Tomoko Ohnishi,

and P. Leslie Dutton 1580 Enhancement of monoclonal antibodies against HLA-

intermediates.

witz, Eckard Munck, and Jack Peisach

A2 is due to antibody bivalency. Nicholas J . Holmes and Peter Parham

1587 Human placental 17P-estradiol dehydrogenase and 20a-hydroxysteroid dehydrogenase. Studies with 6P- bromoacetoxyprogesterone.

James L. Thomas and Ronald C. Strickler

1591 Cysteinesulfinate metabolism. Altered partitioning between transamination and decarboxylation following administration of P-methyleneaspartate.

Owen W. Griffith

elephant myoglobin.

mero-Herrera

1599 Interaction of ligands with the distal glutamine in

David E. Bartnicki, Hiroshi Mizukami, and A. E . Ro-

1603 Partial purification and characterization of a uracil- DNA glycosylase from wheat germ.

Peter Blaisdell and Huber Warner

1610 Oxidative ring fission of the naphthoquinones lapachol and dichloroallyl lawsone by Penicillium notatum.

Sharee L. Otten and John P. Rosazza

1614 Proteolytic interconversion of electrophoretic variants of the enzyme rhodanese.

Paul Horowitz and Karen Falksen 1619 Interaction of 3’-[3H]-2‘-chloro-2’-deoxyuridine 5’-tri-

phosphate with ribonucleotide reductase from Lacto- bacillus leichmannii.

Blakley JoAnne Stubbe, Georgianna Smith, and Raymond L.

1625 Mechanism of ribonucleoside diphosphate reductase from Escherichia coli. Evidence for 3°C-H bond cleavage.

JoAnne Stubbe, Mark Ator, and Thomas Krenitsky

1632 Vitamin A deprivation selectively lowers uridine nucleotide pools in cultured Sertoli cells.

Daniel D. Carson and William J . Lennarz 1637 Photoaffinity labeling of the chick progesterone recep-

tected after removal of proteolytic interference. tor proteins. Similar hormone binding domains de-

Muriel E. Birnbaumer, William T. Schrader, and Bert W. O’Malley

1645 Steady state kinetic evidence for an acyl-enzyme intermediate in reactions catalyzed by bovine spleen cathepsin B.

Andrew S. Bajkowski and Allen Frankfater 1650 The pH dependency of bovine spleen cathepsin B-cat-

p-nitrophenol to water and dipeptide nucleophiles. alyzed transfer of N“-benzyloxycarbonyl-L-lysine from

Comparisons with papain. Andrew S. Bajkowski and Allen Frankfater

1656 Anion effects on in vitro sarcoplasmic reticulum function. The relationship between anions and calcium flux.

Van Winkle, and Mark L. Entman Alice Chu, Charlotte A. Tate, Roger J. Bick, W. Barry

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Charge determination of proteins with polyelectrolyte titration.

Dieter Horn and Claus-Chr. Heuck

Cordycepin analogues of 2-5A and its derivatives. Chemical synthesis and biological activity.

Johnston, and Paul F. Torrence Hiroaki Sawai, Jiro Imai, Krystyna Lesiak, Margaret I .

Guanosine 5’-triphosphate, 3’-diphosphate 5’-phospho- hydrolase. Purification and substrate specificity.

Identification of multiple species of calmodulin messenger RNA using a full length complementary DNA.

Lisette Lagace, T. Chandra, Savio L. C. Woo, and An- thony R. Means

The interaction of vincristine with calf brain tubulin.

Akira Hara and Jose Sy

V. Prakash and Serge N. Timasheff

Noncollagenous proteins of rat compact bone.

son, and Ulf Svanberg Anders Linde, Mats Jontell, Ted Lundgren, Bengt Nil-

Growth factor-stimulated protein phosphorylation in Go/GI-arrested fibroblasts. T w o distinct classes of growth factors with potentiating effects.

Jean-Claude Chambard, Arlette Franchi, Alphonse Le Cam, and Jacques Pouyssegur

Release of transcript and template during transcrip- tion termination at the trp operon attenuator.

C-22-substituted steroid derivatives as substrate analogues and inhibitors of cytochrome P-450.,,.

Ammonia formation in isolated rat liver mitochondria.

J. Pease Kathryn F. LaNoue, Anton C. Schoolwerth, and Andrew

Glutamate transport in rat kidney mitochondria. Anton C. Schoolwerth, Kathryn F. LaNoue, and Wanda

J . Hoover

Resonance Raman spectra of the heme in leghemoglo- bin. Evidence for the absence of ruffling and the influ- ence of the vinyl groups.

Stephen B. Kong, and Kevin M. Smith Denis L. Rousseau, Mark R. Ondrias, Gerd N . LaMar,

Photoaffinity labeling of catechol 0-methyltransferase with 8-azido-S-adenosylmethionine.

Kirk, and Boyd E. Haley Ivan I. Kaiser, Dawn M. Kladianos, Edward A. Van

The catalytic mechanism of glutathione reductase as derived from x-ray diffraction analyses of reaction intermediates.

Vivian Berlin and Charles Yanofsky

Joel J . Sheets and Larry E. Vickery

Emil F. Pai and George E. Schulz

Stereochemical course of DNA hydrolysis by nuclease s1.

Barry V. L. Potter, Paul J . Romaniuk, and Fritz Eckstein

Cooperative effects in the interaction between melittin and phosphatidylcholine model membranes. Studies by temperature scanning densitometry.

Peter Laggner Monika Posch, Ulrike Rakusch, Christa Mollay, and

Diacylglycerol kinase from pig brain. Purification and phospholipid dependencies.

Effect of tunicamycin, an inhibitor of protein glycosyl- ation, on the biological properties of acetylcholine receptor in cultured muscle cells.

Regulation of ornithine aminotransferase mRNA levels in rat kidney by estrogen and thyroid hormone.

Evidence that inhibitors of anion exchange induce a transmembrane conformational change in Band 3.

Zan G. Macara, Scot Kuo, and Lewis C. Cantley

ride antigen of type I1 group B Streptococcus. Structural determination of the capsular polysaccha-

Harold J. Jennings, Karl-Gunnar Rosell, Ewa Katzenel- lenbogen, and Dennis L. Kasper

Hide0 Kanoh, Hiroaki Kondoh, and Teruo On0

Joav Prives and Dafna Bar-Sagi

Mike M. Mueckler and Henry C. Pitot

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Proton nuclear magnetic resonance studies of the ligation states of the monomeric ferricytochrome c' from Rhodopseudomnas palustris. Modulation of axial histidine bonding via variable proton donation.

Bartsch J. Timothy Jackson, Gerd N. La Mar, and Robert G.

Primary structures of three human pancreas peptides with growth hormone-releasing activity.

Brazeau, William B. Wehrenberg, and Roger Guillemin Fred S. Esch, Peter Bohlen, Nicholas C. Ling, Paul E.

Pyruvylated glycolipids from Mycobacterium smeg- rnatis. Structures of two oligosaccharide components.

tices. New assay procedures and inhibition by sulfhy- Release of granule contents from sea urchin egg cor-

dryl-modifying reagents.

Purification and properties of NADP-dependent for- mate dehydrogenase from Clostridium thermoaceti- cum, a tungsten-selenium-iron protein.

Lars G. Ljungdahl Zsamu Yamamoto, Takashi Saiki, Shiu-Mei Liu, and

ATP-dependent acidification of membrane vesicles isolated from purified rat liver lysosomes. Acidification activity requires phosphate.

Modification of the Neurospora crassa plasma membrane [H+]-ATPase with N,N'-dicyclohexylcarbodi- imide.

Siawusch Saadat and Clinton E. Ballou

John G. Haggerty and Robert C. Jackson

Donald L. Schneider

Michael R. Sussman and Carolyn W. Slayman The resistance to tryptic hydrolysis of peptide bonds adjacent to W,N-dimethyllysyl residues.

D e novo purine synthesis in human lymphocytes. Par- tial co-purification of the enzymes and some properties

Louis Poncz and Dorr G. Dearborn

of the pathway. ~~

Eric McCairns, Dale Fahey, Dorit Sauer, and Peter B. Rowe

Inactivation of the 2-ketoglutarate and pyruvate dehydrogenase complexes of beef heart by branched chain keto acids.

Ronald H. Jackson and Thomas P. Singer

Subunit structure of electron transfer flavoprotein.

Frerman Martha C. McKean, Joe D. Beckrnann, and Frank E.

Binding of Clostridium perfringens '251-labeled A-toxin to erythrocytes.

Biochemical evidence for the presence of two vitamin D-dependent calcium-binding proteins in mouse kidney.

thieu Anne-Cecile Delorme, Jean-Louis Danan, and Henri Ma-

The structure of the complex type oligosaccharide from rabbit hepatic binding protein. A re-examination.

Partial purification and reconstitution of the Na+-D- glucose ~~ cotransport protein from pig renal proximal

Colette Joliuet-Reynaud and Joseph E. Alouf

Mark Lowe and Bo Nilsson

tubules. Hermann Koewsell, Helmut Menuhr. Zlze Ducis, and

The0 F. Wissmulier

M13 procoat protein into liposomes which have bac- Reconstitution of rapid and asymmetric assembly of

terial leader peptidase.

Studies on adenosine triphosphate transphosphorylases. Human isoenzymes of adenylate kinase: isolation and physicochemical comparison of the crystal- line human ATP-AMP transphosphorylases from mus-

Yoshiko Ohno-Zwashita and William Wickner

cle and liver.

chael C. Cress, and Minoru Hamada

" .

Stephen A. Kuby, Gerald Fleming, Asta Frischat, Mi-

Identification and expression of a cloned yeast heat shock gene.

David B. Finkelstein and Susan Strausberg

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Inactivation of human coagulation factor V by activated protein C.

Koji Suzuki, Johan Stenflo, Bjorn Dahlbiick, and Bo Teodorsson

Multiple forms of DNA-dependent RNA polymerases in Xenopus laevis. Rapid purification and structural and immunological properties.

David R. Engelke, Barkur S. Shastry, and Robert G. Roeder

Multiple forms of DNA-dependent RNA polymerases inxenopus laevis. Properties, purification, and subunit structure of class I11 RNA polymerases.

Peptide intermediates in the degradation of cellular proteins. Bestatin permits their accumulation in mouse liver in vivo.

Robert G. Roeder

Violeta Botbol and Oscar A. Scornik

Phosphorylation of brain cytosol proteins. Effects of phospholipids and calmodulin.

Judith C. Juskeuich, Donald M. Kuhn, and Walter LOU- enberg

Initiation factor and ribosome levels are coordinately controlled in Escherichia coli growing at different rates.

J. Greg Howe and John W. B. Hershey

Bacillus thuringiensis crystal protein gene. Transcriptional and translational start sites for the

Whiteley Hing Cheung Wong, H. Ernest Schnepf, and H. R.

The fate of an N-formylated chemotactic peptide in stimulated human granulocytes. Subcellular fraction- ation studies.

Painter, Larry A..Sklar, and Charles G. Cochrane Algirdas J. Jesaitis, Joseph R. Naemura, Richard G.

Terminal adenylation in the synthesis of RNA by Q/3 replicase.

Carl Dobkin, and Donald R. Mills James N. Bausch, Fred Russell Kramer, Eleanor Miele,

The rfd gene codes for ADP-L-glycero-D-mannohep- tose-6-epimerase. An enzyme required for lipopolysac- charide core biosynthesis.

Functional domains in introns. RNA processing intermediates in cis- and trans-acting mutants in the penultimate intron of the mitochondrial gene for cytochrome b.

Deborah K. Hanson, Helene J . Klapper, Philip S. Perlman, Mary Rose Lamb, Paul Q. Anziano, Kent R. Glaus,

and Henry R. Mahler Molecular motion and conformation of cholesteryl esters in reconstituted high density lipoprotein by deuterium magnetic resonance.

d l , and Robert J . Cushley Yashpal I . Parmar, Heiner Gorrissen, Stephen R. Was-

Proteolytic processing of presecretory proteins is required for development of biological activities in pan- creatic exocrine proteins.

A role of calcium-activated phospholipid-dependent protein kinase in human platelet activation. Compari- son of thrombin and collagen actions.,.

Kimihiko Sano, Yoshimi Takai, JunJl Yamanishi, and Yasutomi Nishizuka

thelia1 cells in culture. Sulfated proteoglycans synthesized by vascular endo-

Atsuhiko Oohira, Thomas N. Wight, and Paul Bornstein Purification and characterization of yeast topoisom- erase I.

yechan, and Lucy M. S. Chang Gianfranco Badaracco, Paolo Pleuani, William T. Ru-

Properties of purified enzymes induced by pathogenic drug-resistant mutants of herpes simplex virus. Evi- dence for virus variants expressing normal DNA PO- lymerase and altered thymidine kinase.

Graham Darby Brendan A. Larder, David Derse, Yung-Chi Cheng, and

William G. Coleman, Jr.

George Scheele and Russell Jacoby

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2034 Intracellular transloeation and metabolism of a flu- orescent phosphatidic acid analogue in cultured fibroblasts.

Richard E. Pagano, Kenneth J. Longmuir, and Ona C. Martin

2041 Epidermal growth factor and epidermal growth factor receptor-dependent phosphorylation of a M, = 34,000 protein substrate for pp60”‘“.

Pradip Ghosh-Dastidar and C. Fred Fox

2045 Purification and properties of T4 phage thymidylate synthetase produced by the cloned gene in an ampliti- cation vector.

Marlene Belfort, Alan Moelleken, Gladys F. Maley, and Frank Maley

2052 Increased synthesis of glutathione S-transferases in response to anticarcinogenic antioxidants. Cloning and measurement of messenger RNA.

William R. Pearson, Jolene J. Windle, John F. Morrow, Ann M. Benson, and Paul Talalay

GENERAL

Submit all manuscripts in triplicate to Editor, The Journal of Biological Chemistry 9650 Rockville Pike Bethesda, MD 20814, U.S.A.

Accepted manuscripts will be published with the implicit under- standing that the author(s) will pay a charge per page (miniprint supplement excluded). Current page charges may be obtained by contacting the JBC Office. Under exceptional circumstances, when no source of grant or other support exists, the author(s) may apply, at the time of submission, for a grant-in-aid to Chairman, Publi- cations Committee, American Society of Biological Chemists, Inc., 9650 Rockville Pike, Bethesda, MD 20814. All such applications must be countersigned by an appropriate institutional official stating that no funds are available for page charges.

Queries on matters of general editorial policy, requests for reprints of the “Instructions to Authors,” or of the “Editorial Policy and Practices,” or for permission to reproduce any part of a previously published article should be directed to the Journal Editorial Ofice in Bethesda, telephone 301-530-7150.

ADDITIONS AND CORRECTIONS

2063 Cloning, partial sequencing, and expression of glycer- aldehyde-3-phosphate dehydrogenase gene in chick embryonic heart muscle cells. Vol. 257 (1982) 9872- 9877.

H. H. Arnold, H. Domdey, K. Wiebauer, K. Datta, and M. A. Siddiqui

2063 Hydrophobic binding properties of the lectin from lima beans (Phaseolus lunatus). Vol. 257 (1982) 11274- 11277.

David D. Roberts and Irwin J. Goldstein

2064 Structure of chicken hypothalamic luteinizing hormone-releasing hormone. I. Structural determination on partially purified material. Vol. 257 (1982) 10722- 10728.

Judy A. King and Robert P. Millar

INFORMATION

The Journal of Biological Chemistry publishes papers on a broad range of topics of interest to biochemists. The views expressed are those of the author(s) and not of The Journal of Biological Chem- istry or the American Society of Biological Chemists.

Address all correspondence and orders relative to subscriptions and backcopiesto: THE JOURNALOF BIOLOGICAL CHEMISTRY,~~~ EASTPRESTON STREET,BALTIMORE, MD21202,U.S.A.,telephone 301-528-4255. Requests for termination of subscriptions will be honored with proportional refunds less a $10 cancellation charge. Allow at least six weeks for address changes. Claims for replacement copies must be received within four months of the issue date.

Published semimonthly by the American Society of Biological Chemists, Inc. (Annual volume: United States, $310; other countries, $360. Single copy: United States, $14.00, other countries, $16.00.) Subscriptions are entered on a calendar year basis only. Special rates are available to members, graduate students, and postdoctoral fellows for personal use only. Qualifying forms are available from the Bethesda Office. Second class postage paid at Baltimore, MD 21202, U.S.A., and at additional mailing offices.

Vol. 258, No. 3 The Journal of Biological Chemistry February 10, 1983

CONTENTS Arranged by Subject Categories

CARBOHYDRATES, LIPIDS, AND OTHER NATURAL PRODUCTS

1544 '"0 labeling of adenine nucleotide a-phosphoryls in Dlatelets. Contribution bv Dhosuhodiesterase-cata-

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rides through the use of the nuclear Overhauser ef- Sequence determination for N-linked oligosaccha-

fect.

Oxidative ring fission of the naphthoquinones lapachol and dichloroallyl lawsone by Penicillium notatum

Diacylglycerol kinase from pig brain. Purification and phospholipid dependencies.

Structural determination of the capsular polysaccha- ride antigen of type I1 group B Streptococcus.

Harold J. Jennings, Karl-Gunnar Rosell, Ewa Katze- nellenbogen, and Dennis L. Kasper Pyruvylated glycolipids from Mycobacterium smeg- matis. Structures of two oligosaccharide components.

The structure of the complex type oligosaccharide from rabbit hepatic binding protein. A re-examination.

The rfd gene codes for ADP-L-glycero-D-mannohep- tose-6-epimerase. An enzyme required for lipopoly- saccharide core biosynthesis.

William G. Coleman, Jr. Sulfated proteoglycans synthesized by vascular en- dothelial cells in culture.

stein Atsuhiko Oohira, Thomas N. Wight, and Paul Born-

Jean-Robert Brisson and Jeremy P. Carver

Sharee L. Otten and John P. Rosazza

Hideo Kanoh, Hiroaki Kondoh, and Teruo On0

Siawusch Saadat and Clinton E. Ballou

Mark Lowe and Bo Nilsson

CELL BIOLOGY AND METABOLISM Effects of insulin on phenylephrine-induced activation of phosphorylase and phosphatidylinositol turnover in isolated hepatocytes.

tide. Partial loss of sensitivity to guanosine-5'-(P,y- Opiate receptor binding affected by guanine nucleo-

imido) triphosphate evident in uitro.

Pertussis toxin inhibits enkephalin stimulation of GTF'ase of NG108-15 cells.

Drusilla L. Burns, Erik L. Hewlett, Joel Moss, and Martha Vaughan Phosphofructokinase mutants of yeast. Biochemistry and genetics.

Proteolytic conversion of arginine-vasopressin and oxytocin by brain synaptic membranes. Characteri- zation of formed peptides and mechanisms of proteolysis.

The action of insulin on hepatic fructose 2,6-hisphos-

Andrew P. Thomas and John R. Williamson

Jan S. Rosenbaum and Wolfgang Sadee

Zita Lobo and Pabitra K. Maitra

J . Peter H. Burbach and Jos L. M. Lebouille

phate metabolism. Simon J . Pilkis, Ted D. Chrisman, M. Raafat El-Magh-

rabi. Anne Colosia. Eric Fox, Jo Pilkis, and Thomas H. Clais NADPH-dependent production of Oxy radicals by purified components of the rat liver mixed function oxidase system. I. Oxidation of hydroxyl radical scav- enging agents.

Gary W. Winston and Arthur I. Cederbaum NADPH-dependent production of Oxy radicals by pu-

idase system. 11. Role in microsomal oxidation of rified components of the rat liver mixed function ox-

ethanol.

metabolism in primary cultures of adult rat hepato- cy-Adrenergic regulation of enzymes of amino acid

cytes. Chiseko Noda, Toshikazu Nakamura, and Akira Ichi-

hara


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iyzed hydrolysis of CAMP." * A

Timothy F. Walseth, John E. Gander, Susan J. Eide, Thomas P. Krick, and Nelson D. Goldberg Cysteinesulfinate metabolism. Altered partitioning between transamination and decarboxylation following administration of 19-methyleneaspartate.

Vitamin A deprivation selectively lowers uridine nucleotide pools in cultured Sertoli cells.

Growth factor-stimulated protein phosphorylation in Go/G1-arrested fibroblasts. Two distinct classes of growth factors with potentiating effects.

Jean-Claude Chambard, Arlette Franchi, Alphonse Le Cam, and Jacques Pouyssegur Ammonia formation in isolated rat liver mitochondria.

Kathryn F. LaNoue, Anton C. Schoolwerth, and Andrew J . Pease Glutamate transport in rat kidney mitochondria.

J. Hoover Anton C. Schoolwerth, Kathryn F. LaNoue, and Wanda

Release of granule contents from sea urchin egg cor- tices. New assay procedures and inhibition by sulfhy- dryl-modifying reagents.

De nouo purine synthesis in human lymphocytes. Par- tial co-purification of the enzymes and some properties of the pathway.

Eric McCairns, Dale Fahey, Dorit Sauer, and Peter B. Rowe Binding of Clostridium perfringens 1251-Iabeled A- toxin to erythrocytes.

Biochemical evidence for the presence of two vitamin D-dependent calcium-binding proteins in mouse kidney.

Anne-Cecile Delorme, Jean-Louis Danan, and Henri Mathieu

proteins. Bestatin permits their accumulation in Peptide intermediates in the degradation of cellular

mouse liver in uiuo.

The fate of an N-formylated chemotactic peptide in stimulated human granulocytes. Subcellular fraction- ation studies.

Painter, Larry A . Sklar, and Charles G. Cochrane Algirdas J. Jesaitis, Joseph R. Naemura, Richard G.

Proteolytic processing of presecretory proteins is required for development of biological activities in pan- creatic exocrine proteins.

A role of calcium-activated phospholipid-dependent protein kinase in human platelet activation. Compar- ison of thrombin and collagen actions.

Yasutomi Nishizuka Kimihiko Sano, Yoshimi Takai, Junji Yamanishi, and

Intracellular translocation and metabolism of a flu- orescent phosphatidic acid analogue in cultured fibroblasts.

Martin Richard E. Pagano, Kenneth J . Longmuir, and Ona C.

Epidermal growth factor and epidermal growth factor receptor-dependent phosphorylation of a M, = 34,000 protein substrate for pp60"".

Owen W. Griffith

Daniel D. Carson and William J. Lennarz

John G. Haggerty and Robert C. Jackson

Colette Joliuet-Reynaud and Joseph E. Alouf

Violeta Botbol and Oscar A. Scornik

George Scheele and Russell Jacoby

Pradip Ghosh-Dastidar and C. Fred Fox

ENZYMOLOGY Oligomeric structure and catalytic activity of G type Casein kinase. Isolation of the two subunits and re- naturation experiments.

Claude Cochet and Edmond M. Chambaz 6

Indicates a Communication

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Stereospecific elimination of hydrogen at C-10 in ei-

triene Cs. cosapentaenoic acid during the conversion to leuko-

Separation and identification of type 1 and type 2 protein phosphatases from rabbit reticulocyte lysates.

J. Gordon Foulkes, Vivian Ernst, and Daniel H. Levin Turnover of cytochrome c oxidase from Paracoccus denitrificans.

Resolution and reassociation of three distinct components from pig heart phosphoprotein phosphatase.

Teiji Zmaoka, Michinori Imazu, Hirofumi Usui, Nobu- hisa Kinohara, and Masao Takeda Human placental l7D-estradiol dehydrogenase and 20a-hydroxysteroid dehydrogenase. Studies with 6~-bromoacetoxyprogesterone.

Interaction of 3’-[3H]-2’-chloro-2’-deoxyuridine 5’-triphosphate with ribonucleotide reductase from Lacto- bacillus leichmannii

Blakley JoAnne Stztbbe, Georgianna Smith, and Raymond L.

Mechanism of ribonucleoside diphosphate reductase from Escherichia coli. Evidence for 3°C-H bond

Sven Hammarstrom

Juergen K. V. Reichardt and Quentin H. Gibson

James L. Thomas and Ronald C. Strickler

cleavage. JoAnne Stubbe, Mark Ator, and Thomas Krenitsky

Steady state kinetic evidence for an acyl-enzyme intermediate in reactions catalyzed by bovine spleen cathepsin B.

The pH dependency of bovine spleen cathepsin B-

from p-nitrophenol to water and dipeptide nucleo- catalyzed transfer of N“benzyloxycarbony1-L-lysine

philes. Comparisons with papain.

Guanosine 5’-triphosphate, 3’-diphosphate 5”phos- phohydrolase. Purification and substrate specificity.

C-22-substituted steroid derivatives as substrate analogues and inhibitors of cytochrome P-450,,,.

Stereochemical course of DNA hydrolysis by nuclease s1.

Barry V. L. Potter, Paul J. Romaniuk, and Fritz Eck- stein.

mate dehydrogenase from Clostridium thermoaceti- Purification and properties of NADP-dependent for-

cum, a tungsten-selenium-iron protein.

Lars G. Ljungdahl Zsamu Yamamoto, Takashi Saiki, Shiu-Mei Liu, and

The resistance to tryptic hydrolysis of peptide bonds adjacent to N’, N-dimethyllysyl residues.

hydrogenase complexes of beef heart by branched Inactivation of the 2-ketoglutarate and pyruvate de-

chain keto acids.

lases. Human isoenzymes of adenylate kinase: isola- Studies on adenosine triphosphate transphosphory-

tion and physicochemical comparison of the crystal- line human ATP-AMP transphosphorylases from muscle and liver.

chael C. Cress, and Minoru Hamada Stephen A . Kuby, Gerald Fleming, Asta Frischat, Mi-

Phosphorylation of brain cytosol proteins. Effects of phospholipids and calmodulin.

Lovenberg Judith C. Juskeuich, Donald M. Kuhn, and Walter

Purification and characterization of yeast topoisom- erase I.

yechan, and Lucy M. S. Chang Gianfranco Badaracco, Paolo Pleuani, William T. Ru-

Properties of purified enzymes induced by pathogenic drug-resistant mutants of herpes simplex virus. Evi- dence for virus variants expressing normal DNA po- lymerase and altered thymidine kinase.

Graham Darby Brendan A. Larder, Dauid Derse, Yung-Chi Cheng, and

Andrew S. Bajkowski and Allen Frankfater

Andrew S. Bajkowski and Allen Frankfater

Akira Hara and Jose Sy

Joel J . Sheets and Larry E. Vickery

Louis Poncz and Dorr G. Dearborn

Ronald H. Jackson and Thomas P. Singer

-1391

*1395

01415

1467

1474

1571

1656

1761

1775

1785

1833

1839

1888

1895

2000

*1379

01383

MEMBRANES AND BIOENERGETICS Labeling of high affinity ATP binding sites on the 53,000- and 160,000-dalton glycoproteins of the sarco lasmic reticulum with the photaffinity probe 8-N3- [a%]*TP.

Kevin P. Campbell and Dauid H. MacLennan Localization of membrane proteins by the use of a photoreactive fatty acid incorporated in vivo into ve- sicular stomatitis virus.

John Capone, Pierre Leblanc, Gerhard E. Gerber, and Hara P. Ghosh Ribose. An oxidation product of glucose 6-phosphate in microsomal fraction.

GTP-dependent anion-sensitive adenylate cyclase in snail ganglia potentiation of neurotransmitter effects.

Philippe Deterre, Henri Gozlan, and Joel Bockaert Measurement of the electrochemical proton gradient in submitochondrial particles.

Inhibition of electron transfer by 3-alkyl-2-hydroxy-

oxidoreductases of Rhodopseudomonas sphaeroides 1,4-naphthoquinones in the ubiquinol-cytochrome c

and mammalian mitochondria. Interaction with a ubiquinone-binding site and the Rieske iron-sulfur

Yukinobu Hino and Shigeki Minakami

Edward A. Berry and Peter C. Hinkle

cluster. Katsumi Matsuura, John R. Bowver, Tomoko Ohnishi,

and P. Leslie Dutton Anion effects on in vitro sarcoplasmic reticulum function. The relationship between anions and calcium flux.

Van Winkle, and Mark L. Entman Alice Chu, Charlotte A. Tate, Roger J . Bick, W. Barry

tin and phosphatidylcholine model membranes. Stud- Cooperative effects in the interaction between melit-

ies by temperature scanning densitometry. Monika Posch, Ulrike Rakusch, Christa Mollay, and

Peter Laggner Effect of tunicamycin, an inhibitor of protein glyco-

receptor in cultured muscle cells. sylation, on the biological properties of acetylcholine

Joav Prives and Dafna Bar-Sagi Evidence that inhibitors of anion exchange induce a transmembrane conformational change in Band 3.

Zan G. Macara, Scot Kuo, and Lewis C. Cantley ATP-dependent acidification of membrane vesicles isolated from purified rat liver lysosomes. Acidifica- tion activity requires phosphate.

Modification of the Neurospora c m s a plasma membrane [H’I-ATPase with N,W-dicyclohexylcarbodi- imide.

Partial purification and reconstitution of the Na+-D- glucose cotransport protein from pig renal proximal tubules.

The0 F. Wissmuller Hermann Koepsell, Helmut Menuhr, Ilze Ducis, and

Reconstitution of rapid and asymmetric assembly of M13 procoat protein into liposomes which have bac- terial leader peptidase.

Molecular motion and conformation of cholesteryl esters in reconstituted high density lipoprotein by deuterium magnetic resonance.

sall, and Robert J . Cushley Yashpal Z. Parmar, Heiner Gorrissen, Stephen R. Was-

Donald L. Schneider

Michael R. Sussman and Carolyn W. Slayman

Yoshiko Ohno-Zwashita and William Wickner

NUCLEIC ACIDS, PROTEIN SYNTHESIS, AND MOLECULAR GENETICS

ferent autoantigens. Sequential association of nucleolar 7-2 RNA with dif-

Detection of a nucleolar 7-2 ribonucleoprotein and a

ies from patients with scleroderma. cytoplasmic 8-2 ribonucleoprotein with autoantibod-

Ram Reddy, Eng M. Tan, Dale Henning, Katsu Nohga, and Harris Busch

Carl Hashimoto and Joan A. Steitz


8

-1387

-1399

01423

1456

1536

1559

1603

1671

1684

1714

1781

1908

1921

1932

1954

1960

1978

1991

Messenger RNA for the 73,000-dalton poly(A)-binding protein occurs as translationally repressed mRNP in duck reticulocytes.

Kinsey Maundrell, Maria Tereza Imaizumi-Scherrer, E. Stuart Maxwell, Olivier Civelli, and Klaus Scherrer Nucleotide sequences of two pea cDNA clones encoding the small subunit of ribulose 1,5-bisphosphate

thylakoid polypeptide. carboxylase and the major chlorophyll a/b-binding

Gloria Coruzzi, Richard Broglie, Anthony Cashmore, and Nam-Hai Chua Ribonuclease D is not essential for the normal growth of Escherichia coli or bacteriophage T4 or for the biosynthesis of a T4 suppressor tRNA.

Deutscher Richard T. Blouin, Richard Zaniewski, and Murray P.

The interaction in vitro of the intermediate filament protein vimentin with naturally occurring RNAs and DNAs.

Peter Traub, W. James Nelson, Siegfried Kuhn, and Constantin E. Vorgias Purification and characterization of DNase VIII. A 5’- 3’ directed exonuclease from human placental nuclei.

Mossbauer study of iron bleomycin and its activation intermediates.

witz, Eckard Miinck, and Jack Peisach Richard M. Burger, Thomas A. Kent, Susan Band Hor-

Partial purification and characterization of a uracil- DNA glycosylase from wheat germ.

Chemical synthesis and biological activity. Cordycepin analogues of 2-5A and its derivatives.

Hiroaki Sawai, Jiro Imai, Krystyna Lesiak, Margaret I. Johnston, and Paul F. Torrence Identification of multiple species of calmodulin messenger RNA using a full length complementary DNA.

Lisette Lagace, T. Chandra, Savio L. C. Woo, and Anthony R. Means Release of transcript and template during transcrip- tion termination at the trp operon attenuator.

Regulation of ornithine aminotransferase mRNA levels in rat kidney by estrogen and thyroid hormone.

Identification and expression of a cloned yeast heat shock gene.

Multiple forms of DNA-dependent RNA polymerases in Xenopus laevis. Rapid purification and structural and immunological properties.

David R . Engelke, Barkur S. Shastry, and Robert G. Roeder

Multiple forms of DNA-dependent RNA polymerases in Xenopus laevis. Properties, purification, and subunit structure of class 111 RNA polymerases.

Initiation factor and ribosome levels are coordinately controlled in Escherichia coli growing at different rates.

Antonia M. Pedrini and Lawrence Grossman

Peter Blaisdell and Huber Warner

Vivian Berlin and Charles Yanofsky

Mike M. Mueckler and Henry C. Pitot

David B. Finkelstein and Susan Strausberg

Robert G. Roeder

J . Greg Howe and John W. B. Hershey

Bacillus thuringiensis crystal protein gene. Transcriptional and translational start sites for the

Whiteley Hing Cheung Wong, H. Ernest Schnepf, and H. R.

Terminal adenylation in the synthesis of RNA by Qp replicase.

James N. Bausch, Fred Russell Kramer, Eleanor Miele, Carl Dobkin, and Donald R. Mills

Functional domains in introns. RNA processing intermediates in cis- and trans-acting mutants in the penultimate intron of the mitochondrial gene for cytochrome b.

Mary Rose Lamb, Paul Q. Anziano, Kent R. Glaus, Deborah K. Hanson, Helene J . Klapper, Philip S. Perlman, and Henry R. Mahler

2045

2052

-1407

1450

1565

1580

1599

1614

1637

1665

1689

1698

1740

1747

1752

1799

1806

1866

1914

Purification and properties of T4 phage thymidylate

fication vector. synthetase produced by the cloned gene in an ampli-

Marlene Belfort, Alan Moelleken, Gladys F. Maley, and Frank Maley

response to anticarcinogenic antioxidants. Cloning Increased synthesis of glutathione S-transferases in

and measurement of messenger RNA.

Ann M. Benson, and Paul Talalay William R. Pearson, Jolene J . Windle, John F. Morrow,

PROTEIN CHEMISTRY AND STRUCTURE A novel thrombin-reactive protein complex in human platelets.

Mohanathasan Chelladurai, Nancy G. Fossett. and Pankaj Ganguly The dynamics of formation of a collagen-phosphopho- ryn conjugate in relation to the passage of the min- eralization front in rat incisor dentin.

Thyrotropin-releasing hormone-diphtheria toxin-re-

hydrophobic domain in toxin entry. lated polypeptide conjugates. Potential role of the

Patricia Bacha, John R . Murphy, and Seymour Reichlin Enhancement of monoclonal antibodies against HLA- A2 is due to antibody bivalency.

George D. Maier, Joseph H. Lechner, and Arthur Veis

Nicholas J. Holmes and Peter Parham Interaction of ligands with the distal glutamine in elephant myoglobin.

David E. Bartnicki, Hiroshi Mizukami, and A. E. Ro- mero-Herrera

iants of the enzyme rhodanese. Proteolytic interconversion of electrophoretic var-

Photoaffinity labeling of the chick progesterone receptor proteins. Similar hormone binding domains detected after removal of proteolytic interference.

W. O’Malley Muriel E. Birnbaumer, William T. Schrader, and Bert

lyte titration. Charge determination of proteins with polyelectro-

Dieter Horn and Claus-Chr. Heuck The interaction of vincristine with calf brain tubulin.

V. Prakash and Serge N. Timasheff Noncollagenous proteins of rat compact hone.

Anders Linde, Mats Jontell, Ted Lundgren, Bengt Nil- son, and Ulf Svanberg

bin. Evidence for the absence of ruffling and the influ- Resonance Raman spectra of the heme in leghemoglo-

ence of the vinyl groups.

Stephen B. Kong, and Kevin M. Smith Denis L. Rousseau, Mark R. Ondrias, Gerd N. LaMar,

Photoaffinity labeling of catechol O-methyltransferase with 8-azido-S-adenosylmethionine.

Kirk, and Boyd E . Haley Ivan I. Kaiser, Dawn M. Kladianos, Edward A. Van

The catalytic mechanism of glutathione reductase as derived from x-ray diffraction analyses of reaction intermediates.

Proton nuclear magnetic resonance studies of the ligation states of the monomeric ferricytochrome c’ from Rhodopseudomonas palustris. Modulation of axial histidine bonding via variable proton donation.

Bartsch J. Timothy Jackson, Gerd N. La Mar, and Robert G.

Primary structures of three human pancreas peptides with growth hormone-releasing activity.

Brazeau, William B. Wehrenberg, and Roger Guillemin Fred S. Esch, Peter Bohlen, Nicholas C. Ling, Paul E .

Subunit structure of electron transfer flavoprotein. Martha C. McKean, Joe D. Beckmann, and Frank E.

Frerman

vated protein C. Inactivation of human coagulation factor V by acti-

Koji Suzuki, Johan Stenflo, Bjorn Dahlback, and Bo Teodorsson

Paul Horowitz and Karen Falksen

Emil F. Pai and Georg E. Schulz


vol. 258, no. 3 biological chemistry · vol. 258, no. 3 the journal of february 10, 1983 biological...

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