vol. 21 the journal november biological chemistry · port system in eacherichia coli. measurement...

Vol. 258, No. 21 The Journal of November 1 0,1983

Biological Chemistry Copyright 0 1983 by the American Society of Biological Chemists, Inc., 428 East Preston St., Baltimore, Md. 21202 U S A .

CONTENTS*

COMMUNICATIONS 12745 Post-translational modification of the fourth compo-

nent of complement. Sulfation of the a-chain.

12749 Amino acid sequence at the reactive site of human (11-

David R. Karp

antichymotrypsin. M. Morii and James Travis

12753 Molecular cloning of human adenosine deaminase gene sequences.

Alexander F. Markham, Gail A. Bruns, Sabra C. Goff, and Stuart H. Orkin, Peter E. Daddona, Donna S. Shewach,

William N . Kelley

12757 Subunit composition of oligomeric human von Wille- brand factor.

Kirby, and David M. Livingston Dennis C. Lynch, Theodore S. Zimmerman, Edward P.

12761 X-ray absorption spectroscopic studies of high valent iron porphyrins. Horseradish peroxidase compounds I and I1 and synthetic models.

James E. Penner-Hahn, Thomas J . McMurry, Mark Ren-

Davis, Alan L. Balch, John T. Groves, John H. Dawson, and ner, Lechoslaw Latos-Grazynsky, Kim Smith Eble, Ian M.

Keith 0. Hodgson 12765 Lithium ion-sugar cotransport via the melibiose trans-

port system in Eacherichia coli. Measurement of Li+ transport and specificity.

Tomofusa Tsuchiya, Mari Oho, and Sumiko Shiota-Niiya 2768 The sequence of the nucleotides at the a-sarcin cleav-

age site in rat 28 S ribosomal ribonucleic acid. Yuen-Ling Chan, Yaeta Endo, and Ira G. Wool

2771 Resonance Raman studies of beef heart aconitase and a bacterial hydrogenase.

Spiro, Rona R. Ramsay, and Thomas P. Singer Michael K. Johnson, Roman S. Czernuszewicz, Thomas G.

2775 Deglycosylated human chorionic gonadotropin. An antagonist to desensitization and down-regulation of the gonadotropin receptor-adenylate cyclase system.

R. Victor Rebois and Peter H. Fishman

1

12779 5’-Bromoacetamido-5’-deoxyadenosine. A novel re- agent for labeling adenine nucleotide sites in proteins.

12783 Effects of parathyroid hormone and vitamin D on the

Bhaskar R. Samarzt and Frederick Sweet

renal vitamin K-dependent carboxylating system. Peter I. Karl and Paul A. Friedman

12787 In vivo flux between DhosDhocreatine and adenosine triphosphate determined by two-dimensional phos- Dhorous NMR.

retti Robert S. Balaban, Howard L. Kantor, and James A. Fer-

12790 Evidence for direct methyl transfer in betaine: homocysteine S-methyltransferase.

William M. Awad, Jr., Philip L. Whitney, William E. Skiba, John H. Mangum, and Marilyn S. Wells

12793 Post-translational modification of a tryptophan biosynthetic enzyme. Precursor-product relationships in vivo and carbon-energy source dependence.

Raymond D. Mosteller and Ruth V. Goldstein 12797 Products derived from 5J3.11-eicosatrienoic acid by

Barbara A. Jakschik, Aubrey R. Morrison, and Howard the 5-lipoxygenase-leukotriene pathway.

Sprecher

12801

- 12804

12810

12816

12821

12827

12835

12842

12847

12857

12865

12868

12876

12881

12886

12895

Complete structure of lipid A obtained from the lipopolysaccharides of the heptoseless mutant of Safmo- nella typhimurium.

Kuni Takayama, Nilofer Qureshi, and Paolo Mascagni

RNA polymerase I1 from wild type and a-amanitin- resistant strains of Caenorhabditis elegans.

Thomas Sanford, Miriam Golomb, and Donald L. Riddle

The importance of the Escherichia coli ribosomal protein L16 for the reconstitution of the peptidyl-tRNA hydrolysis activity of peptide chain termination.

Warren P. Tate, Hartmut Schulze, and Knud H. Nierhaus

The Escherichia coli ribosomal protein L11 sup- presses release factor 2 but promotes the release factor 1 activities in peptide chain termination.

Warren P. Tate, Hartmut Schulze, and Knud H. Nierhaus

Solubilization of triolein and cholesteryl oleate in egg phosphatidylcholine vesicles.

James A. Hamilton, Kurt W. Miller, and Donald M. Small

Interactions between xenobiotics that increase or decrease the levels of cytochrome P-450 isozymes in rabbit lung and liver.

Robertson, and Richard M. Philpot Cosette J. Serabjit-Singh, Phillip W. Albro, lain G. C.

Spectral and electron transfer properties of Sepharose 6MB-immobilized cytochrome c.

Melvin A. Schafer and George S. Wilson Evidence of 2-aminopurine-cytosine base mispairs involving two hydrogen bonds.

Myron F. Goodman and Robert L. Ratliff

Characterization of low buoyant density dermatan sulfate proteoglycans synthesized by rat ovarian granulosa cells in culture.

Masaki Yanagishita and Vincent C. Hascall

Characterization of heparan sulfate proteoglycans synthesized by rat ovarian granulosa cells in culture.

Statistical limits in Scatchard analysis.


Henry A. Feldman

Synthesis and coenzyme A-dependent esterification of cholesterol in rat intestinal epithelium. Differences in cellular localization and mechanisms of regulation.

Edwrd F. Stange, Keith E. Suckling, and John M. Dietschy

Differential effects of protease inhibitors on 1,26- dihydroxyvitamin Da receptors.

and June E. Bishop Anthony W. Norman, Willi Hunziker, Marian R. Walters,

Rapid vesicle reconstitution of alprenolol-Sepharose- purified B1-adrenergic receptors. Interaction of the purified receptor with N..

Daniel J . Kelleher, Abbas Rashidbaigi, Arnold E. Ruoho, and Gary L. Johnson

Characterization of ascorbic acid transport by adren- omedullary chromaffin cells. Evidence for Na+-dependent co-transport.

Emanuel J . Diliberto, Jr., Grace Dean Heckmnn, and Ale- jandro J. Daniels

Amino acid sequence of ovine 6-phosphogluconate dehydrogenase.

Alan Carm and John E. Walker

* The CONTENTS arranged by Subject Categories will be found immediately following these CONTENTS. Full Instructions to Authors will be found in (1983) J. Biol. Chem. 258, 1-11, and reprints may be obtained from the editorial office

2

3

12907

12912

12920

12929

12934

12940

12947

12952

12957

12958

12967

12976

12982

12988

12994

13002

13008

13014

ATP-citrate lyase phosphorylation in rat adipose tissue.

Dominick L. Pucci, Seethala Ramakrishna, and William B. Benjamin

(Na,K)-ATPase-mediated cation pumping in cultured

rocholate transport and characterization of its rela- rat hepatocytes. Rapid modulation by alanine and tau-

tionship to intracellular sodium concentration. Rebecca W. Van Dyke and Bruce F. Schurschmidt

The isolation and identification of two new metabo- lites of 25-hydroxyvitamin Da produced in the kidney.

In vivo ‘‘N NMR studies of regulation of nitrogen assimilation and amino acid production by Brevibac- terium lactofermentum.

Glenville Jones, May Kung, and Kenichi Kano

Nurit Haran, Zvi E. Kahana, and Aviva Lapidot

Characterization of proteolysis fragments of aspartokinase 1:homoserine dehydrogenase I. Fluorescence and circular dichroism studies.

Fluorescence studies of threonine-promoted confor-

strate analogue 2’(3’)-0-(2,4,6-trinitrophenyl)adeno- mational transitions in aspartokinase I using the sub-

sine 5”triphosphate.

Philip L. McMahon and Mark Takahashi

Karen E. Broglie and Mark Takahashi

Position of ester groups in the lipid A backbone of lipopolysaccharides obtained from Salmonella typhi- murrum.

Nilofer Qureshi, Kuni Takayama, David Helkr, and Cath- erine Fenselau

The amino acid sequence of D-ribose-binding protein from Escherichia coli K12.

James M. Groarke, Walter C. Mahoney, John N. Hope,

Mark A. Hermodson Clement E. Furlong, Frank T. Robb, Howard Zalkin, and

Appendix. The amino acid sequence of D-ribose-binding protein from Salmonella typhimurium ST1.

Gail Karns Buckenmeyer and Mark A. Hermodson

Differential turnover of methyl groups on methyl-

erythrocytes. accepting membrane proteins of irreversibly sickled

Gloria A. Green, Suresh C. S i k h , and Vijay K. Kalra

Partial purification and properties of a murine plas- macytoma glucosyltransferase.

Paul Gold and Michael Green

Monoclonal antibodies directed against mammalian RNA polymerase I. Identification of the catalytic center.

pel, Dean A. Stetler, and Ernest D. Marquez Kathleen M. Rose, Kathleen A. Maguire, John N. D. Wur-

Evidence for a small catalytic domain in the adenylate cyclase from Salmonella typhimurium.

phate N-acetylglucosamine synthesis in delipidated Restoration by phospholipids of dolichol pyrophos-

rat lung microsomes.

Terry K. Leib and John A. Gerlt

Pamela L. Plouhar and Roger K. Bretthauer

Insulin-mediated modifications of myocardial lipoprotein lipase and lipoprotein metabolism.

Vahouny Patricia O’Looney, Mary Vander Maten, and George V.

Kinetic evidence for interacting active sites in the Neurospora crassa plasma membrane ATPase.

Barry J . Bowman

Studies of the ferredoxin from Thermus thermophilus. Russ Hi&, Tatsuro Yoshida, George E . T a r , Charles H.

Williams, Jr., Martha L. Ludwig, James A. Fee, Thomas A. Kent, Boi Hanh Huynh, and Eckard Munck

Characterization of 3-iron ferredoxins by means of the linear electric field effect in EPR.

Jack Peisach, Helmut Beinert, Mark H. Emptage, W. B. Mirns, James A . Fee, William H. Orme-Johnson, Alan R. Rendina, and Nannette R. Orme-Johnson

13017 Inhibition of hormone-stimulated adenylate cyclase activity after altering turkey erythrocyte phospho- lipid composition with a nonspecific lipid transfer pro-

binding from adenylate cyclase activation. tein. Phosphatidylinositol uncouples catecholamine

Charles C. McOsker, Gregory A. Weiiand, and Donald B. Zilversmit

13027 Conditions influencing formation of 16:0/16:0 molecular species in membrane phospholipids of Esche- richia coli.

Neungton, Brian Wattenberg, and Davld F. Silbert Cindy Lau, David L. A. Greenway, Carl E. Freter, Neolobol

13034 Altered acyltransferase activity in Escherichia coli associated with mutations in acyl coenzyme A synthe- tase.

David L. A. Greenway and David F. Silbert 13043 Kinetics of activation of acetyl-coA carboxylase by

citrate. Relationship to the rate of polymerization of the enzyme.

Narlin B. Beaty and M. Daniel Lane 13051 The polymerization of acetyl-coA carboxylase.

Narlin B. Beaty and M. Daniel Lane 13056 Activation of spermatozoan adenylate cyclase by a low

molecular weight factor in porcine seminal plasma. Naomichi Okamura and Yoshiki Sugita

13063 Order of metal binding in metallothionein. Kirk B. Nielson and Dennis R. Winge

13070 Novel form of DNA polymerase a associated with DNA primase activity of vertebrates. Detection with mouse stimulating factor.

Tatsuo Yagura, Tomoko Kozu, Takeshi Seno, Mineo Sa- myoshi, Sota Hiraga, and Hiroshi Nagano

3076 Structure of the 5’ terminus of hen oviduct lysozyme

William H. Eschenfeldt, Beth Genya Cohen, and Robert E. messenger ribonucleic acid.

Rhoads 3082 Biological activities of phosphodiester linkage isomers

Krystyna Lesiak, Jiro Imai, Georgia Floyd-Smith, and Paul of 2-5A.

F. Torrence 13089

13094

13100

13111

13116

13120

13127

13134

13141

Epidermal growth factor stimulates tyrosine phosphorylation of the myosin regulatory light chain from - smooth muscle.

Byron Gallis. Arthur M. Edelman. John E. Casnellie, and Edwin G. Krebs A simple and sensitive procedure for measuring iso- tope fluxes through ion-specific channels in heterog- enous populations of membrane vesicies.

The sequence of the NHp-terminal 204-residue frag- ment. of the heavy chain of rabbit skeletal muscle myosin.

Ascorbic acid and catecholamine secretion from cultured chromaffin cells.

Zinder Mark Levine, Anthony Asher, Harvey Pollard, and Oren

Cycloheximide causes accumulation of insulin receptors at the cell surface of cultured fibroblasts.

Robert E. Fellows Ranjana Kadle, Valerie G. Kalter, Mohan K. Raizada, and

Pre-steady state 8-lactamase kinetics. The trapping of a covalent intermediate and the interpretation of pH rate profiles.

Immunological detection of microtubule poison-induced conformational changes in tubulin.

nucleosides but not on others. Sugar-free growth of mammalian cells on some ribo-

Inhibitory regulation of adenylyl cyclase in the absence of stimulatory regulation. Requirements and kinetics of guanine nucleotide-induced inhibition of the cyc- S49 adenylyl cyclase.

Haim Garty, Bernardo Rudy, and Steven J . D. Karlish

Siu Wing Tong and Marshall Elzinga

Ellen G. Anderson and R. F. Pratt

Janet L. Morgan and Brian S. Spooner

Burton M. Wice and David E. Kennel1

John D. Hildebrandt and Lutz Birnbaumer

4

13148

13155

13160

13166

13172

13178

13185

13193

13199

13205

13213

13221

13230

13236

13243

13250

13258

13262

Identification of the target amino acids in the site- specific inactivation of triose phosphate isomerase by ferrate anion.

Janusz Steczko, Mark Hermodson, Bernard Axelrod, and Elzbieta Dziembor-Kentzer

Structure and reactivity of cysteine residues in mito- chondrial serine hydroxymethyltransferase.

Francisco Gauilanes, Darrell Peterson, Bryan Bullis, and La Verne Schirch

sis bouina. A biosynthetic role for carnitine in the yeast Torulop-

Ron K. Emaus and Loran L. Bieber

Photoaffinity labeling of lactose synthase with a UDP- galactose analogue.

Timothy K. Lee, Lee-Jun C. Wong, and Shan S. Wong

Turnover of excess hemoglobin a chains in 8-thalas- semic cells is ATP-dependent.

Joseph R. Shueffer

Unconventional reading of the glycine codons.

Lagerkvist

Amplification of adenosine deaminase gene sequences in deoxycoformycin-resistant rat hepatoma cells.

Tore Samwlsson, Torsten Anberg, Thomas Boren, and Ulf

Stephen W. Hunt, IZZ and Patricia A. Hoffee

Thermodynamics of the binding of L-arabinose and of D-galactose to the L-arabinose-binding protein of Escherichia coli.

Harumi Fukada, Julian M. Sturtevant, and Florante A. Quiocho

A central role for cyclic AMP, but not progesterone, in luteinizing hormone receptor down-regulation in the granulosa cell.

Complexes of Escherichia coli primase with the replication origin of G4 phage DNA.

Ralph H. Schwa11 and Gregory F. Erickson

Mark M. Stayton and Arthur Kornberg

The proteolytic substructure of light meromyosin. Lo-

strength insolubility of myosin. calization of a region responsible for the low ionic

Laszlo Nyitray, Gabor Mocz, Laszlo Szilugyi, Miklos Balint, Renne Chen Lu, Anna Wong, and John Gergely

and 17 stabilize nucleosome core particles. Nonhistone nuclear high mobility group proteins 14

Arthur E. Paton, Elizabeth Wilkinson-Singley, and Donald E. Olins

Cytochrome oxidase subunit 2 gene in Neurospora crassa mitochondria.

Giuseppe Macino and Giorgio Morelli

The Chinese hamster cell emetine resistance gene. Analysis of cDNA and genomic sequences encoding ribosomal protein S14.

Noboru Nakamichi, Douglas D. Rhoads, and Donald J . Roufa

tion of glycoproteins of the mouse egg’s extracellular Role of asparagine-linked oligosaccharides in secre-

coat. Richard J . Roller and Paul M. Wassarman

Repeating covalent structure and protective immu- nogenicity of native and synthetic polypeptide frag-

protective and nonprotective epitopes with mono- ments of type 24 streptococcal M protein. Mapping of

clonal antibodies.

David L. Hasty Edwin H. Beachey, Jerome M. Seyer, James B. Dale, and

LexA protein is a repressor of the colicin El gene.

Nakazawa, and Roger Brent

Isolation and characterization of a 115,000-dalton matrix-associated glycoprotein from chick aorta.

Giorgio M. Bressan, Ines Castelluni, Alfonso Colombatti, and Din0 Volpin

Yousuke Ebina, Yoshiyuki Takahara, Fumio Kishi, Atsushi

13268

13273

13281

13287

13292

13299

13307

13314

13321

13328

13331

13340

13347

13353

13359

13370

Keratin biosynthesis in normal mouse epithelia and in squamous cell carcinomas. mRNA-dependent altera- tions of the primary structure of distinct keratin subunits in tumors.

Jurgen Schweizer and Hermelita Winter

Preparation and characterization of membrane fractions enriched in outer and inner envelope membranes

munochemical analyses. from spinach chloroplasts. I. Electrophoretic and im-

Maryse Anne Block, Albert-Jean Dorne, Jacques Joyard, and Roland Douce

tions enriched in outer and inner envelope membranes Preparation and characterization of membrane frac-

ization. from spinach chloroplasts. 11. Biochemical character-

Maryse Anne Block, Albert-Jean Dorne, Jacques Joyard, and Roland Douce

zymatic activity in isolated sera urchin mitotic appa- Identification of nonmitochondrial creatine kinase en-

ratus.

David Cole Robert B. Silver, Mallory S. Saft, Alice R. Taylor, and R.

Significance of phosphorylation of phosphofructoki- nase.

Shigetaka Kitajima, Ryuzo Sakakibara, and Kosaku Uyeda

RNA levels in methotrexate-resistant KB cells. 5-Fluorouracil modulation of dihydrofolate reductase

Bruce J. Dolnick and John J. Pink

The assembly of an H2Az,H2Bz,H3,H4 hexamer onto DNA under conditions of physiological ionic strength.

Michael J . Ellison and David E. Pulleyblank

which form in vitro under conditions of physiological Internal structure of discrete nucleohistone complexes

ionic strength. Michael J . Ellison and David E. Pulleyblank

Pathways of assembly of nucleohistone complexes formed in vitro under physiological conditions. Impli- cations for the structure of the nucleosome.


Proteins of the Bacillus stearothermophilus ribosome. Crystallization of proteins L30 and 55.

Krzysztof Appelt, Stephen W. White, and Keith S. Wilson

Regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity in avian myeloblasts. Mode of action of 25-hydroxycholesterol.

Richard D. Tanakn, Peter A. Edwards, Show-Fung Lan, and Alan M. Fogelman

Precursor proteins are transported into mitochondria in the absence of proteolytic cleavage of the additional sequences.

Craig Zwizinski and Walter Neupert

Coated vesicles from rat liver and calf brain contain lysosomal enzymes bound to mannose 6-phosphate receptors.

Corinne Horn Campbell and Leonard H . Rome

Site selection and structure of DNA-linked RNA primers synthesized by the primosome in phage 9x174 DNA replication in vitro.

Tohru Ogawa, Ken-ichi Arai, and Tuneko Okazaki

Role of the regulatory light chains in skeletal muscle actomyosin ATPase and in minifilament formation.

Sarkis S. Margossian, Ashok K. Bhun, and Henry S. Slayter

Identification and purification of an adenylate kinase- associated protein that influences the thermolability of adenylate kinase from a temperature-sensitive adk mutant of Escherichia-coli.

Ronald J . Huss and Mzchnel Glaser

5

13377 Hormonal regulation of the phosphorylation of glyco- 13391 Biosynthetic and structural properties of endothelial gen synthase in perfused rat heart. Effects of insulin, cell type VI11 collagen. catecholamines, and glucagon. Helene Sage, Beat Trueb, and Paul Bornstein

Dona1 A. W a k h Chidambaram Ramachandran, Karen L. Angelos, and

13384 A 174-kilodalton ATPase/dATPase polypeptide and a glycoprotein of apparently identical molecular weight are common but distinct components of higher eukar- 13402 Isolation of a five-polypeptide cytochrome b - f complex yotic nuclear structural protein subfractions. from spinach chloroplasts. Vol. 258 (1983) 10348-

Fisher Robert D. Clark and Geoffrey Hind

ADDITIONS AND CORRECTIONS

Miguel Berrios, Adele J . Filson, Gimter Blobel, and Paul A. 10354.

GENERAL INFORMATION FOR 1984

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Vol. 258, No. 21 The Journal of Biological Chemistry November 10, 1983

CONTENTS Arranged by Subject Categories

CARBOHYDRATES, LIPIDS, AND OTHER NATURAL PRODUCTS

12929 In vivo "N NMR studies of regulation of nitrogen assimilation and amino acid production by Brevi-

012797

e12801

12821

12847

12857

12868

12920

12988

12994

-12775

12787

12827

12876

12886

12907

Products derived from 5,8,1l-eicosatrienoic acid by the 5-lipoxygenase-leukotriene pathway.

Barbara A. Jakschik, Aubrey R. Morrison, and Howard Sprecher Complete structure of lipid A obtained from the lipopolysaccharides of the heptoseless mutant of Sal- monella typhimurium.

Kuni Takayama, Nilofer Qureshi, and Paolo Mascagni Solubilization of triolein and cholesteryl oleate in egg phosphatidylcholine vesicles.

Small James A. Hamilton, Kurt W. Miller, and Donald M.

Characterization of low buoyant density dermatan sulfate proteoglycans synthesized by rat ovarian granulosa cells in culture.

Characterization of heparan sulfate proteoglycans synthesized by rat ovarian granulosa cells in culture.

Synthesis and coenzyme A-dependent esterification of cholesterol in rat intestinal epithelium. Differ- ences in cellular localization and mechanisms of regulation.

Eduard F. Stange, Keith E. Suckling, and John M. Dietschy. The isolation and identification of two new metab- olites of 25-hydroxyvitamin D3 produced in the kidney.

Restoration by phospholipids of dolichol pyrophos- phate N-acetylglucosamine synthesis in delipidated rat lung microsomes.

Pamela L. Plouhar and Roger K. Bretthauer Insulin-mediated modifications of myocardial lipoprotein lipase and lipoprotein metabolism.

Vahouny Patricia O'Looney, Mary Vander Maten, and George V.



Glenuille Jones, May Kung, and Kenichi Kano

CELL BIOLOGY AND METABOLISM Deglycosylated human chorionic gonadotropin. An antagonist to desensitization and down-regulation of the gonadotropin receptor-adenylate cyclase system.

I n vivo flux between phosphocreatine and adenosine triphosphate determined by two-dimensional phos- phorous NMR.

Robert S. Balaban, Howard L. Kantor, and James A. Ferretti Interactions between xenobiotics that increase or decrease the levels of cytochrome P-450 isozymes in

R. Victor Rebois and Peter H. Fishman

rabbit lung and liver.

Robertson, and Richard M. Philpot Cosette J. Serabjit-Singh, Phillip W . Albro, lain G. C.

Differential effects of protease inhibitors on 1,25- dihydroxyvitamin Ds receptors.

ters, and June E. Bishop Anthony W. Norman, Willi Hunziker, Marian R. Wal-

Characterization of ascorbic acid transport by ad-

dependent co-transport. renomedullary chromaffin cells. Evidence for Na+-

Emanuel J. Diliberto, Jr., Grace Dean Heckman, and Alejandro J. Daniels ATP-citrate lyase phosphorylation in rat adipose tissue.

Dominick L. Pucci, Seethala Ramakrishna, and William B. Benjamin

12947

13056

13111

13116

13127

13134

13160

13199

13243

13287

13331

13347

13370

13384

812779

bacterium lactofermentum.

Position of ester groups in the lipid A backbone of

phimurium. lipopolysaccharides obtained from Salmonella ty-

Catherine Fenselau Nilofer Qureshi, Kuni Takayama, David Heller, and

Activation of spermatozoan adenylate cyclase by a low molecular weight factor in porcine seminal plasma.

Ascorbic acid and catecholamine secretion from cultured chromaffin cells.

Zinder Mark Leuine, Anthony Asher, Harvey Pollard, and Oren

Cycloheximide causes accumulation of insulin receptors at the cell surface of cultured fibroblasts.

and Robert E. Fellows Ranjam Kadle, Valerie G. Kalter, Mohan K. Raizada,

Immunological detection of microtubule poison-induced conformational changes in tubulin.

Sugar-free growth of mammalian cells on some ri- bonucleosides but not on others.

A biosynthetic role for carnitine in the yeast Toru- lopais bovina.

A central role of cyclic AMP, but not progesterone, in luteinizing hormone receptor down-regulation in the granulosa cell.

Role of asparagine-linked oligosaccharides in secretion of glycoproteins of the mouse egg's extracellular coat.

enzymatic activity in isolated sea urchin mitotic Identification of nonmitochondrial creatine kinase

apparatus.

David Cole Robert B. Silver, Mallory S. Saft, Alice R. Taylor, and R.

Regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity in avian myeloblasts. Mode of action of 25-hydroxycbolestero1.

Richard D. Tanaka, Peter A. Edwards, Show-Fung Lan, and Alan M. Fogelman Coated vesicles from rat liver and calf brain contain lysosomal enzymes bound to mannose 6-phosphate receptors.

Identification and purification of an adenylate kinase-associated protein that influences the thermolability of adenylate kinase from a temperature- sensitive adk mutant of Escherichia coli.

A 174-kilodalton ATPase/dATPase polypeptide and a glycoprotein of apparently identical molecular weight are common but distinct components of higher eukaryotic nuclear structural protein subfractions.

Miguel Berrios, Adele J . Filson, Gunter Blobel, and Paul A. Fisher

Nurit Haran, Zvi E. Kahana, and Auiua Lapidot

Naomichi Okamura and Yoshiki Sugita

Janet L. Morgan and Brian S. Spooner

Burton M. Wice and David E. Kennel1

Ron K. Emaus and Loran L. Bieber

Ralph H. Schwa11 and Gregory F. Erickson

Richard J . Roller and Paul M. Wassarman

Corinne Horn Campbell and Leonard H. Rome

Ronald J. Huss and Michael Gloser

ENZYMOLOGY 5'-Bromoacetamido-5'-deoxyadenosine. A novel re- agent for labeling adenine nucleotide sites in proteins.

Bhaskar R. Samant and Frederick Sweet

6 Indicates a Communication

7

012783

012790

012793

12865

12934

12940

12967

12982

13043

1305 1

13089

13120

13141

13148

13155

13205

13292

13377

Effects of parathyroid hormone and vitamin D on the renal vitamin K-dependent carboxylating system.

Peter I. Karl and Paul A. Friedman Evidence for direct methyl transfer in betaine: homocysteine S-methyltransferase.

Skiba, John H. Mangum, and Marilyn S. Wells William M. Awad, Jr., Philip L. Whitney, William E.

Post-translational modification of a tryptophan biosynthetic enzyme. Precursor-product relationships in vivo and carbon-energy source dependence.

Statistical limits in Scatchard analysis.

Characterization of proteolysis fragments of aspar-

cence and circular dichroism studies. tokinase 1:homoserine dehydrogenase I. Fluores-

Fluorescence studies of threonine-promoted confor-

substrate analogue 2’(3’)-0-(2,4,6-trinitrophe- mational transitions in aspartokinase I using the

ny1)adenosine 5’-triphosphate.

Partial purification and properties of a murine plas- macytoma glucosyltransferase.

Paul Gold and Michael Green Evidence for a small catalytic domain in the adenylate cyclase from Salmonella typhimurium.

citrate. Relationship to the rate of polymerization of Kinetics of activation of acetyl-coA carboxylase by

the enzyme.

The polymerization of acetyl-coA carboxylase.

Epidermal growth factor stimulates tyrosine phosphorylation of the myosin regulatory light chain from smooth muscle.

and Edwin G. Krebs Byron Gallis, Arthur M. Edelman, John E. Casnellie,

Pre-steady state 8-lactamase kinetics. The trapping

pH rate profiles. of a covalent intermediate and the interpretation of

Inhibitory regulation of adenylyl cyclase in the absence of stimulatory regulation. Requirements and kinetics of guanine nucleotide-induced inhibition of the cyc- S49 adenylyl cyclase.

Identification of the target amino acids in the site-

by ferrate anion. specific inactivation of triose phosphate isomerase

Elzbieta Dziembor-Kentzer J a n w z Steczko, Mark Hermodson, Bernard Axelrod, and

Structure and reactivity of cysteine residues in mi- tochondrial serine hydroxymethyltransferase.

La Verne Schirch Francisco Gavilnnes, Darrell Peterson, Bryan Bullis, and

Complexes of Escherichia coli primase with the replication origin of G4 phage DNA.

kinase. Significance of phosphorylation of phosphofructo-

Shigetaka Kitajim, Ryuzo Sakakibara, and Kosaku Uyeda

cogen synthase in perfused rat heart. Effects of in- Hormonal regulation of the phosphorylation of gly-

sulin, catecholamines, and glucagon.

Dona1 A. Walsh Chidambaram Ramachandran, Karen L. Angelos, and

Raymond D. Mosteller and Ruth V. Goldstein

Henry A. Feldman

Philip L. McMahon and Mark Takahashi

Karen E. Broglie and Mark Takahashi

Terry K. Leib and John A. Gerlt

Narlin B. Beaty and M. Daniel Lane

Narlin B. Beaty and M. Daniel Lane

Ellen G. Anderson and R. F. Pratt

John D. Hildebrandt and Lutz Birnbaumer

Mark M. Stayton and Arthur Kornberg

MEMBRANES AND BIOENERGETICS *12765 Lithium ion-sugar cotransport via the melibiose

transport system in Escherichia coli. Measurement of Li+ transport and specificity.

Tomofusa Tsuchiya, Mari Oho, and Sumiko Shiota-Niiya

Indicates a Communication

12835 Spectral and electron transfer properties of Sephar- ose 6MB-immobilized cytochrome c.

Melvin A. Schafer and George S. Wilson 12881 Rapid vesicle reconstitution of alprenolol-Sephar-

ose-purified &-adrenergic receptors. Interaction of the purified receptor with N..

Daniel J . Kelleher, Abbas Rashidbaigi, Arnold E. Ruoho, and Gary L. Johnson

12912 (Na,K)-ATPase-mediated cation pumping in cul-

and taurocholate transport and characterization of tured rat hepatocytes. Rapid modulation by alanine

tion. its relationship to intracellular sodium concentra-

Rebecca W. Van Dyke and Bruce F. Scharschmidt 12958 Differential turnover of methyl groups on methyl-

accepting membrane proteins of irreversibly sickled erythrocytes.

Gloria A. Green, Suresh C. Sikka, and Vijay K. Kalra 13002

13017

13027

13034

13094

13273

13281

13340

~~

Kinetic evidence for interacting active sites in the Neurospora crassa plasma membrane ATPase.

Inhibition of hormone-stimulated adenylate cyclase activity after altering turkey erythrocyte phospho- lipid composition with a nonspecific lipid transfer protein. Phosphatidylinositol uncouples catecholamine binding from adenylate cyclase activation.

Chnrles C. McOsker, Gregory A . Weilnnd, and Donald B. Zilversmit Conditions influencing formation of 16:0/16:0 molecular species in membrane phospholipids of Esch- erichia coli.

lobo1 Neungton, Brzan Wattenberg, and Daurd F. Silbert Cindy L a , David L. A . Greenway, Carl E . Freter, Neo-

Altered acyltransferase activity in Escherichia coli associated with mutations in acyl coenzyme A syn- thetase.

A simple and sensitive procedure for measuring iso- tope fluxes through ion-specific channels in heter- ogenous populations of membrane vesicles.

Haim Garty, Bernard0 Rudy, and Steven J . D. Karlish Preparation and characterization of membrane

branes from spinach chloroplasts. I. Electrophoretic fractions enriched in outer and inner envelope mem-

and immunochemical analyses.

and Roland Douce Maryse Anne Block, Albert-Jean Dorne, Jacques Joyard,

Preparation and characterization of membrane

branes from spinach chloroplasts. 11. Biochemical fractions enriched in outer and inner envelope mem-

characterization.

and Roland Douce Maryse Anne Block, Albert-Jean Dorne, Jacques Joyard,

Precursor proteins are transported into mitochon-

additional sequences. dria in the absence of proteolytic cleavage of the

Barry J. Bowman

David L. A. Greenway and David F. Silbert

Craig Zwizinski and Walter Neupert

NUCLEIC ACIDS, PROTEIN SYNTHESIS, AND MOLECULAR GENETICS

812753 Molecular cloning of human adenosine deaminase gene sequences.

Alexander F. Markhnm, Gail A. Bruns, Sabra C. Goff, and Stuart H. Orkin, Peter E. Daddona, Donna S. Shewach,

William N . Kelley 012768 The sequence of the nucleotides at the a-sarcin

cleavage site in rat 28 S ribosomal ribonucleic acid. Yuen-Ling Chan, Yaeta Endo, and Ira C. Wool

12804 RNA polymerase I1 from wild type and a-amanitin- resistant strains of Caenorhabditis elegans.

Thomas Sanford, Miriam Golomb, and Donald L. Riddle 12810 The importance of the Escherichia coli ribosomal

protein L16 for the reconstitution of the peptidyl- tRNA hydrolysis activity of peptide chain termination.

haus Warren P. Tate, Hartmut Schulze, and Knud H . Nier-

8

12816

12842

12976

13070

13076

13082

13172

13178

13185

13221

13230

13236

13258

13268

13299

13307

13314

13321

The Escherichia coli ribosomal protein L11 sup- presses release factor 2 but promotes the release factor 1 activities in peptide chain termination.

Warren P. Tate, Hartmut Sehulze, and Knud H. Nier- haus

Evidence of 2-aminopurine-cytosine base mispairs involving two hydrogen bonds.

Monoclonal antibodies directed against mammalian RNA polymerase I. Identification of the catalytic center.

Kathleen M. Rose, Kathleen A. Maguire, John N. D. Wurpel, Dean A. Stetler, and Ernest D. Marque2

Novel form of DNA polymerase a associated with DNA primase activity of vertebrates. Detection with mouse stimulating factor.

Saneyoshi, Sota Hiraga, and Hiroshi Nagano Tatsuo Yagura, Tomoko Kozu, Takeshi Seno, Mineo

Structure of the 5' terminus of hen oviduct lysozyme messenger ribonucleic acid.

E. Rhoads William H. Eschenfeldt, Beth Genya C o k n , and Robert

Biological activities of phosphodiester linkage iso-

Myron F. Goodman and Robert L. Ratlijj

mers of 2-5A.

Paul F. Torrence Krystyna Lesiak, Jiro Imai, Georgia Floyd-Smith, and

Turnover of excess hemoglobin a chains in 8-thalas- semic cells is ATP-dependent.

Unconventional reading of the glycine codons.

Ulf Lagerkvist

Amplification of adenosine deaminase gene se- guences in deoxycoformycin-resistant rate hepa-

Joseph R. Shaeffer

Tore Sarnwlsson, Torsten Axberg, Thomas Boren, and

toma cells. Stephen W. Hunt, I I I and Patricia A . Hofjee

Nonhistone nuclear high mobility group proteins 14 and 17 stabilize nucleosome core partlcles.

ald E. Olins Arthur E. Paton, Elizabeth Wilkinson-Singley, and Don-

Cytochrome oxidase subunit 2 gene in Neurospora crassa mitochondria.

Giuseppe Macino and Giorgio Morelli

The Chinese hamster cell emetine resistance gene. Analysis of cDNA and genomic sequences encoding ribosomal protein 514.

Roufa Noboru Nakamichi, Douglas D. Rhoads, and Donald J .

LexA protein is a repressor of the colicin E 1 gene. Yowuke Ebina, Yoshiyuki Takahara, Fumio Kishi, At-

susha Nakazawa, and Roger Brent

Keratin biosynthesis in normal mouse epithelia and in squamous cell carcinomas. mRNA-dependent al-

subunits in tumors. terations of the primary structure of distinct keratin

Jurgen Schweizer and Hermelita Winter

5-Fluorouracil modulation of dihydrofolate reductase RNA levels in methotrexate-resistant KB cells.

Bruce J Dolnick and John J. Pink

onto DNA under conditions of physiological ionic The assembly of an H2A2,H2Bp,H3,H4 hexamer

strength. "


Internal structure of discrete nucleohistone complexes which form in vitro under conditions of physiological ionic strength.

Pathways of assembly of nucleohistone complexes formed in vitro under physiological conditions. Im- plications for the structure of the nucleosome.



13353

e12745

*I2749

12757

012761

-12771

12895

12952

12957

13008

13014

13063

13100

13166

13193

13213

13250

primers synthesized by the primosome in phage Site selection and structure of DNA-linked RNA

$X174 DNA replication in uitro. Tohru Ogawa, Ken-ichi Arai, and Tuneko Okazaki

PROTEIN CHEMISTRY AND STRUCTURE

ponent of complement. Sulfation of the a-chain. Post-translational modification of tbe fourth com-

Amino acid sequence at the reactive site of human a,-antichymotrypsin.

lebrand factor. Subunit composition of oligomeric human von Wil-

Dennis C. Lynch, Theodore S. Zimmerman, Edward P. Kirby, and David M. Livingston X-ray absorption spectroscopic studies of high valent iron porphyrins. Horseradish peroxidase compounds I and I1 and synthetic models.

Renner, Lmhoslaw Latos-Grazynsky, Kim Smith Eble, Ian James E. Penner-Hahn, Thomas J. McMurry, Mark

M . Davis, Atan L. Balch, John 2'. Groues, John H. Dawson, and Keith 0. Hodgson Resonance Raman studies of beef heart aconitase and a bacterial hydrogenase.

C. Spiro, Rona R. Ramsay, and Thomas P. Singer Michael K. Johnson, Roman S.Czernwzewicz, Thomas

Amino acid sequence of ovine 6-phosphogluconate dehydrogeaase.

The amino acid sequence of D-ribose-binding protein from Escherichia coli K12.

Clement E. Furlong, Frank T. Robb, Howard Zalkin, and James M . Groarke, Walter C. Mahoney, John N. Hope,

Mark A. Hermodson Appendix. The amino acid sequence of D-ribose- binding protein from Salmonella typhimurium ST1.

Studies of the ferredoxin from Thermus thermophilus.

Williams,. Jr., Martha L. Ludwig, James A . Fee, Thomas A. Russ Hille, Tatsuro Yoshida, George E. Tarr, Charles H.

Kent, Bor Hanh Huynh, and Eckard Munck Characterization of 3-iron ferredoxins by means of the linear electric field effect on EPR.

Mims, James A. Fee, William H. Orme-Johnson, Alan R. Jack Peisach, Helmut Beinert, Mark H . Emptage, W. B.

Rendina, and Nannette R. Orme-Johnson Order of metal binding in metallothionein.

Kirk B. Nielson and Dennis R. Winge The sequence of the NHz-terminal 204-residue frag- ment-of the heavy chain of rabbit skeletal muscle myosm.

Photoaffinity labeling of lactose synthase with a UDP-galactose analogue.

Timothy K. Lee, Lee-Jun C. Wong, and Shan S. Wong Thermodynamics of the binding of L-arabinose and of D-galactOSe to the L-arabinose-binding protein of Escherichia coli.

Harumi Fukada, Julian M. Sturteuant, and Florante A. Quiocho The proteolytic substructure of light meromyosin. Localization of a region responsible for the low ionic strength insolubility of myosin.

h z t o Nyitray, Gabor Mocz, Laszlo Stilugyi, Miklos Bal- int, Renne Chen Lu, Anna Wong, and John Gergeb Repeating covalent structure and protective immu- nogenicity of native and synthetic polypeptide fragments of type 24 streptococcal M protein. Mapping of protective and nonprotective epitopes with monoclonal antibodies.

Edwin H. Beachey, Jerome M. Seyer, James 8. Dale, and David L. Hasty

David R. Karp

M . Morii an2 James Travis

Alan Carne and John E. Walker

Gail Karns Buckenmeyer and Mark A. Hermodson

Siu Wong Tong and Marshall Elzinga

Indicates a Communication

vol. 21 the journal november biological chemistry · port system in eacherichia coli. measurement...

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