viruses, viroids, and prions 18-2 1. are viruses living or nonliving? viruses are both and neither...
TRANSCRIPT
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Viruses, Viroids, and Prions
18-2
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Are Viruses Living or Are Viruses Living or Nonliving?Nonliving?
• Viruses are both and neither• They have some properties of
life but not others• For example, viruses can be
killed, even crystallized like table salt
• However, they can’t maintain a constant internal state (homeostasis).
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What are Viruses?What are Viruses?
•A virus is a noncellular particle made up of genetic material (DNA) and protein that can invade living cells.
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Discovery of VirusesDiscovery of Viruses•Beijerinck (1897) coined the Latin name “virus” meaning poison•He studied filtered plant juices & found they caused healthy plants to become sick 4
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Tobacco Mosaic VirusTobacco Mosaic Virus
•Wendell Stanley (1935) crystallized sap from sick tobacco plants•He discovered viruses were made of nucleic acid and protein
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SmallpoxSmallpox•Edward Jenner (1796) developed a smallpox vaccine using milder cowpox viruses•Deadly viruses are said to be virulent•Smallpox had been eradicated in the world today, but is now coming back 6
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Viewing VirusesViewing Viruses•Viruses are smaller Viruses are smaller than the smallest cellthan the smallest cell•Measured in Measured in nanometers nanometers•Viruses couldn’t be Viruses couldn’t be seen until the seen until the electron microscope electron microscope was invented in the was invented in the 2020thth century century
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Size of VirusesSize of Viruses
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Viral Viral StructureStructure
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CharacteristicsCharacteristics
• Non living structures• Noncellular• Have a protein coat called the
capsid• Have a nucleic acid core
containing DNA or RNA• Capable of reproducing only
when inside a HOST cell10
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CharacteristicsCharacteristics• Some viruses are
enclosed in a protective envelope
• Some viruses may have spikes to help attach to the host cell
• Most viruses infect only SPECIFIC host cells
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CAPSID
ENVELOPE
DNA
SPIKES
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CharacteristicsCharacteristics•Capsids (coats) are made of individual protein subunits called capsomeres
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CAPSOMERES
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CharacteristicsCharacteristics
•Outside of host cells, viruses are inactive•Lack ribosomes and enzymes needed for metabolism•Use the raw materials and enzymes of the host cell to be able to reproduce
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EBOLA VIRUS
HIV VIRUS
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CharacteristicsCharacteristics
•Some viruses cause disease such as:•Smallpox, measles, mononucleosis, influenza, colds, warts, AIDS, Ebola•Some viruses may cause some cancers like leukemia•Virus-free cells are rare
14MEASLES
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Viral ShapesViral Shapes
•Viruses come in a variety of shapes•Some may be helical shape like the Ebola virus•Some may be polyhedral shapes like the influenza virus•Others have more complex shapes like bacteriophages
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Helical VirusesHelical Viruses
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Polyhedral VirusesPolyhedral Viruses
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Complex VirusesComplex Viruses
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Taxonomy of Taxonomy of VirusesViruses
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Herpes VirusHerpes Virus
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SIMPLEX I and II
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AdenovirusAdenovirus
21COMMON COLD
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Influenza VirusInfluenza Virus
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Chickenpox VirusChickenpox Virus
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Papillomavirus – Papillomavirus – Warts!Warts!
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Used for Virus Used for Virus IdentificationIdentification
• RNA or DNA Virus• Do or do NOT have an
envelope• Capsid shape• HOST they infect
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Bacteriophages
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PhagesPhages•Viruses that attack bacteria are a bacteriophage (phage)•T-phages are a specific class of bacteriophages with icosahedral heads, double-stranded DNA, and tails
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T-phagesT-phages•The most commonly studied T-phages are T4 and T7•They infect E. coli , an intestinal bacteria•Six small spikes at the base of a contractile tail are used to attach to the host cell •Inject viral DNA into cell
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Escherichia coli Bacterium
29T - EVEN PHAGES ATTACK THIS BACTERIUM
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T-Even Bacteriophages
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Diagram of T-4 Diagram of T-4 BacteriophageBacteriophage
•Head with 20 triangular surfaces•Capsid contains DNA•Head & tail fibers made of protein
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Clicker Question - PhagesClicker Question - Phages•A Bacteriophage or a phage is:A virus that infects a __________ cell.
A.PlantB.AnimalC.BacteriaD.ProtistaE.Fungi
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RetrovirusesRetroviruses
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Characteristics of Characteristics of RetrovirusesRetroviruses
•Contain RNA, not DNA•Family Retroviridae•Contain enzyme called Reverse Transcriptase•When a retrovirus infects a cell, it injects its RNA and reverse transcriptase enzyme into the cytoplasm of that cell
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ENZYME
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RetrovirusesRetroviruses
•The enzyme reverse transcriptase (or RTase), which causes synthesis of a complementary DNA molecule (cDNA) using virus RNA as a template
36RTase
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RetrovirusesRetroviruses
•HIV, the AIDS virus, is a retrovirus•Feline Leukemia Virus is also a retrovirus
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Clicker Question!•Retroviruses contain:
A. tRNAB. DNAC. RNAD. rRNAE. mRNA
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Viral Viral ReplicationReplication
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Viral AttackViral Attack
• HOST specific• Humans rarely share viral
diseases with other animals• Eukaryotic viruses usually have
protective envelopes made from the host cell membrane
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5 Steps of Lytic 5 Steps of Lytic CycleCycle
• 1. Attachment to the cell• 2. Penetration (injection) of viral
DNA or RNA• 3. Replication (Biosynthesis) of new
viral proteins and nucleic acids• 4. Assembly (Maturation) of the
new viruses• 5. Release of the new viruses into
the environment (cell lyses)41
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One-step Growth One-step Growth CurveCurve
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Viral LatencyViral Latency•Some viruses have the ability to become dormant inside the cell•Called latent viruses•They may remain inactive for long periods of time (years)•Later, they activate to produce new viruses in response to some external signal•HIV and Herpes viruses are examples 43
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Viral LatencyViral Latency•Once a prophage cell is activated, host cell enters the lytic cell•New viruses form & the cell lyses (bursts)
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INACTIVE STAGE
ACTIVESTAGE
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Latency in Latency in EukaryotesEukaryotes
•Some eukaryotic viruses remain dormant for many years in the nervous system tissues• Chickenpox (caused by the virus Varicella zoster) is a childhood infection•It can reappear later in life as shingles, a painful itching rash limited to small areas of the body
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SHINGLES
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Latency in EukaryotesLatency in Eukaryotes•Herpes viruses also becomes latent in the nervous system
•A herpes infection lasts for a person’s lifetime
•Genital herpes (Herpes Simplex 2)
•Cold sores or fever blisters (Herpes Simplex1) 46
SKIN TO SKIN CONTACT
PASSED AT BIRTH TO BABY
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The Lysogenic CycleThe Lysogenic Cycle
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Virulent VirusesVirulent Viruses
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HOST CELL
LYSES & DIES
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Clicker Question!
• Viruses replicate:
A. On their ownB. With other viruses
by joining piliC. When ever they
want toD. In a host cell only
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• All Viruses ONLY contain RNA.
A. TrueB. False
Clicker Question!Clicker Question!
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Treatment for Treatment for Viral DiseaseViral Disease
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VaccinesVaccines • An attenuated virus is a weakened,
less vigorous virus
• Attenuated virus is capable of stimulating an immune response and creating immunity, but not causing illness
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Other Viral TreatmentsOther Viral Treatments•Interferon are naturally occurring proteins made by cells to fight viruses•Antiviral drugs (AZT)•Protease inhibitors – prevents capsid formation
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Viroids & PrionsViroids & Prions
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ViroidsViroids•Small, circular RNA molecules without a protein coat•Infect plants•Potato famine in Ireland•Resemble introns
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PrionsPrions•Prions are “infectious proteins”• They are normal body proteins that get converted into an alternate configuration by contact with other prion proteins• They have no DNA or RNA•The main protein involved in human and mammalian prion diseases is called “PrP” 56
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Prion Prion DiseasesDiseases•Causes neurons to Causes neurons to
rapidly degenerate.rapidly degenerate.•Examples:Examples:•Mad cow disease Mad cow disease (bovine spongiform (bovine spongiform encephalitis: BSE)encephalitis: BSE)•People in New Guinea People in New Guinea used to suffer from used to suffer from kuru, which they got kuru, which they got from eating the brains from eating the brains of their enemiesof their enemies
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Clicker QuestionPrions can be contracted by…
A: Eating leftovers from the fridge.
B: Eating the brain of an infected animal.
C: Eating meat that has come into contact with the brain or spinal cord of an infected animal.
D: Both B and C
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Clicker Question!•What infectious element
causes Mad Cow Disease?A: PhagesB: PrionsC: ViroidsD: PlasmaE: Virus