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Vector NTI

Nucleotide analyses

Restriction site analysis

Design Primers

Align

Assemble

Vector NTI

Nucleotide analyses

Restriction site analysis

Design Primers

Align

Assemble

Vector NTI SuiteVector NTI Suite

Graphics Pane

Main Menu

Main Toolbar

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Text Pane

Sequence Pane

GenBank/GenPetp

File

Select all sequence →Right-click mouse →Reverse selection

Analyses →ORF

Select ORF→Analyses →Into New Protein →Direct Strand

→Analyses →Restriction Analyses →Restriction Sites

pUC 1926 8 6 bp

A P r

A L P H AP (B L A )

P (L A C )

O R I

Ava I (4 13)

Bam H I (4 18 )

Eco R I (39 7)

H ind III (4 4 8 )

P st I (4 4 0 )

Sm a I (4 15)

X m a I (4 13)

Apa L I (178 )

Apa L I (1121)

Apa L I (236 7)

H um a n h e m o g lob in20 40 bp

N coI (1130 )

N coI (140 9)P s tI (258 ) P s tI (78 5)

P s tI (138 0 )

Sm a I (292)

Sm a I (1237)

Sm a I (1666)

X m a I (290 )

X m a I (1235)

X m a I (1664)A vaI (290 )

A vaI (1153)

A vaI (1235)

A vaI (1664)

pUC 19334 6 bp

A P r

A L P H A

In s e r t fr a g m e n t

P (B L A )

P (L A C )

O R I

Ba m H I (4 18 )

EcoR I (39 7)

H ind III (110 8 )

Nco I (4 6 9 )

P st I (4 4 0 )

Ava I (4 13)

Ava I (724 )

Sm a I (4 15)

Sm a I (726 )

X m a I (4 13)

X m a I (724 )

Bg lI (252)

Bg lI (24 8 0 )

X ba I (4 24 )

X ba I (4 8 8 )

Ap a L I (178 )

Ap a L I (178 1)

Ap a L I (30 27)

by PstI

Select region→Analyses →Primer Design →Find PCR Primers

Select primer→Right-click mouse → Analyses

Select file

Align→AlignX-Align Selected Molecules

Assemble→ContigExpress-Assemble Selected Sequence

Open ContigExpress Project→Project→Add Fragments→From ABI file (no Chinese)

→ Right-click mouse→Camera

→Select sequence → Right-click mouse→Copy sequence…..