uvr-m and uvr-mi, uv air recirculators test report
TRANSCRIPT
UV air recirculators UVR-M and UVR-Mi are equipped with bactericidal lamps (Philips). The main point of the test was to examine the UV-light irradiation impact on various microorganisms types.
UVR-M and UVR-Mi, UV air recirculators Test Report
For majority of microorganisms the energy needed for deactivation was investigated and reported by UVP Inc. Depending on this data it is possible to determine the effectivity of UV air recirculators produced by BioSan Ltd. Throughout knowing the energy produced by instrument and comparing it to energy needed for microorganism deactivation. UVP table is presented below as well as results of measurements performed at BTC laboratory.
Bacteria destruction chartAmount of germicidal, shortwave (254 nm) ultraviolet energy necessary for complete
destruction of various organisms. Presented by UVP inc.
Medical–BiologicalResearch & Technologies
Bacteria organisms Energy: mW seconds per cm2
Other microorganisms
Energy: mW seconds per cm2
Bacillus anthracis 8.7S. enteritidis 7.6B. Megatherium sp. (veg.) 2.5B. Megatherium sp. (spores) 5.2B. parathyphosus 6.1B. subtilis 11.0B. subtilis spores 22.0 List continues on the next page ...
YEAST Saccharomyces ellipsoideus 13.2 Saccharomyces sp. 17.6 Saccharomyces cerevisiae 13.2 Brewer’s yeast 6.6 Baker’s yeast 8.8 Common yeast cake 13.2
List continues on the next page ...
General informationUV radiation affects the viability of microorganisms, as the
absorbed radiation by the molecules leads to photochemical reactions in the structure of DNA/RNA. UV radiation causes adjacent pyrimidine molecules on DNA/RNA to dimerise. Pyrimidine dimers block the reproduction of bacteria. If bacteria can’t reproduce, it dies.
Photochemical reactionThe formation of pyrimidine dimers; as an example taken thymine(source http://www.photobiology.info/)
T T
N N
N N NN
N N
UV
PR
CH3 CH3 CH3 CH3
O O O O
O O O O
T T
UVR-M
UVR-Mi
UVR-M and UVR-Mi, UV air recirculators Test Report
source: www.uvp.com
UVR-M and UVR-Mi, UV air recirculators Test Report
Bacteria organisms Energy: mW seconds per cm2 Other microorganisms Energy:
mW seconds per cm2
Clostridium tetani 22.0Corynebacterium diphtheriae 6.5Eberthella typosa 4.1Escherichlia coli 6.6Micrococcus cadidus 12.3Micrococcus sphaeroides 15.4Mycobacterium tuberculosis 1.0Neisseria catarrhalis 8.5Phytomonas tumefaciens 8.5Proteus vulgaris 6.6Pseudomonas aeruginosa 10.5Pseudomonas fluorescens 6.6S. typhimusium 15.2Salmonella 10.0Sarcina lutea 26.4Sarratia marcescens 6.1Dysentery bacilli 4.2Shigella paradysenteriae 3.2Spirillum rubrum 6.1Staphylococcus albus 5.7Staphylococcus aureus 6.6Streptococcus hemolyticus 5.5Streptococcus lactis 8.8Streptococcus viridans 3.8
MOLD SPORES Penicillium roqueforti 26.4 Penicillium expansum 22.0 Penicillium digitatum 88.0 Aspergillus glaucus 88.0 Aspergillus flavus 99 .0 Aspergillus niger 330.0 Rhisopus nigricans 220.0 Mucor racemosus A 35.2 Mucor racemosus B 35.2 Oospora lactis 11.0
VIRUS Bacteriophage (E. coli) 6.6 Tobacco mosaic 44.0 Influenza 6.6
PROTOZOA Paramecium 200.0 Nematode eggs 92.0 Chlorella vulgaris (algae) 22.0
... List continued from the previous page
Results of Philips 25W bactericidal UV lamp intensity measurements
Distance, cm
UV intensity,mW/cm2
2 20.0
7 10.0
25 4.0
50 2.0
100 5.0
200 1.0
300 0.5
UV
inte
nsit
y, m
W/c
m2
Distance to UV source, cm
Dependence of UV intensity on distance to UV source, one lamp 25 W
18
14
10
6
4
2
20
16
12
8
0 30050 100 150 200 250
42
20
1614
18
1210
86
10 5020 30 40
source: www.uvp.com
Microorganisms sensitivity to UV radiation
160
30
15
25
10
20
5UV
Radi
atio
n le
vel i
n m
W /
cm2 /
sec
Fungi Yeast Vegetative Bacteria
Viruses
UVR-M
Before After
UVR-Mi
UVR-M and UVR-Mi, UV air recirculators Test Report