TYPES OF INSTRUMENTS

UV-VISIBLE SPECTROSCOPY

PRESENTED BY THUSHARA MOHAN, M PHARM, GRACE COLLEGE

1.Single beam UV-Visible spectrophotometer

2.Double beam UV-Visible spectrophotometer

SINGLE BEAMSPECTROPHOTOMETER

SINGLE BEAM INSTRUMENT

5

Source of light

Filter/Mono

chromatorsSample

cells

Detectors

Read out device

CONSTRUCTION

WORKING

DISADVANTAGES

1.Fluctuation

2.Rapid scanning is not possible

DOUBLE BEAM SPECTROPHOTOMETER

CONSTRUCTION

Source of light

Filters/Mono

chromatorsSample cell

Reference cell D1

Read out deviceD2

Beam spliter