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oster 5.0.86

sing AFLP method for linkage mapping of lentilenome

ehnaz Ozatay

Canakkale Onsekiz Mart University, Ezine Vocational School, Turkey

he construction of molecular linkage maps has become an essen-ial tool for plant molecular genetics and breeding research. Therere a lot of markers for making a molecular linkage maps. AFLP,APS, RAPD, ISSR, RFLP and SCAR are the most popular molec-lar markers used in genetics. There is an advantage of AFLP byhich a lot of polymorphism can be obtained. The first linkageap of lentil was constructed by Havey and Muehlbauer (1989).

n this study Li-Cor AFLP Analyser machine and its kits were usedo imagine, analyse and screen markers. Different Taq DNA poly-

erases were used for analysis and one of is selected according toet the best results. This machine has its dying and imagining partsts inside. So, there is no need for additional dying procedure andmagine equipment. Also, classical AFLP method was used how-ver the number of polymorphic bands is not as much as the resultsbtained from Li-Cor Analyser. In this study also different dilutionactors were tried and 1:40 was selected. Finally, 178 polymorhicands were obtained with different primer combinations.

ttp://dx.doi.org/10.1016/j.nbt.2012.08.526

oster 5.0.87

ioreactor systems for large scale micropropagation forruit tree rootstocks

. Orhan Fedakar∗, M. Bayraktar, A. Gurel

Ege University, Engineering Faculty, Bioengineering Department,ornova-Izmir, Turkey

icropropagation is an efficient method for large scale produc-ion of economically important plants. But micropropagation byonventional techniques is not cost effective because of low micro-ropagation rate of plants. Clonal propagation on semi solidedium is also labour-intensive. For solving these production

roblems, bioreactor systems using liquid media have been sug-ested due to the ease of scaling up and controlling physicalnd chemical environment of cultures. Besides these advantageshere are some limited factors for bioreactor systems like highosts of investment and repairing. Contamination is also biggerroblem in bioreactor systems than in semisolid cultures. But theajor disadvantage of bioreactor systems is hyperhydricity. Bet-

er aeration and intermittent plant submergence in the mediumre provided in temporary immersion bioreactors. There are a lotf new patented disposable bioreactor systems using temporarymmersion systems for large scale propagation of different plantypes such as ornamentals, fruit trees, cereals and field crops. Thiseview describes some of new disposable bioreactor systems, RITA,ROWTECH, SETIS and PLANTIMA. Nowadays, micropropaga-

ion of fruit rootstocks is the main income resource of commercialaboratories in Turkey. Bioreactor systems have potential and dis-inct advantages for those, hence development of new bioreactor

tpa

188 www.elsevier.com/locate/nbt

New Biotechnology · Volume 29S · September 2012

ystems for large scale micropropagation will be essential in nearuture.

Keywords: Fruit tree rootstocks; Micropropagation; Liquidultures; Bioreactors; Temporary immersion systems

ttp://dx.doi.org/10.1016/j.nbt.2012.08.527

oster 5.0.88

ydrothermal reaction of by-product lignin generatedn bioethanol production systems using lignocellulosic

aterials to convert bioactive agents

eung-Cheol Lee1,∗, Eun-Kyung Jo1, Yasuyuki Matsushita2,achie Yagami2, Rie Inakoshi2, Norihiro Takamoto2, Kazuhikoukushima2

Department of Food Science and Biotechnology, Kyungnam University,hangwon 631-701, Republic of KoreaGraduate School of Bioagricultural Sciences, Nagoya University, Furo-ho Chikusa-ku Nagoya, Aichi 464-8601, Japan

o utilize the by-product lignin (SAL) that is generated byioethanol production systems that use lignocellulosic materialsith a sulfuric acid catalyst, the antioxidant activity, reducingower, and acetylcholinesterase inhibitory activity of SAL were

nvestigated. Hydrothermal treatment effectively increased theotal phenolic content (TPC) and had positive effects on thentioxidant activity and reducing power. By using model com-ounds, the reaction mechanism of the increase in TPC byydrothermal treatment was determined. The antioxidant activ-

ty, reducing power, and acetylcholinesterase inhibitory activity ofydrothermalized SAL were insensitive to the methoxy group con-

ent, molecular weight, and differences in nucleus species (such asuaiacyl or syringyl). This suggests that the species of lignocellu-osic materials have little relationship to the potential utilizationf SAL as a bioactive agent.

Keywords: Lignin; Hydrothermal reaction; Antioxidant;educing power; Acetylcholinesterase inhibitory activity

ttp://dx.doi.org/10.1016/j.nbt.2012.08.528

oster 5.0.89

he effects of subcritical water treatment on antioxi-ant activity of Saururus chinensis

u-Jung Kim1, Da-Jung Heo1, You-Jin Shin1, Ae-Sun Song1, Jong-wa Lee2, Seung-Cheol Lee1,∗

Department of Food Science and Biotechnology, Kyungnam University,hangwon 631-701, Republic of KoreaDepartment of Food Science and Biotechnology, Andong National Uni-ersity, Andong 760-749, Republic of Korea

aururus chinensis is one of the widely used plants in oriental tra-itional medicine. The hot water (HW) extraction of S. chinensisas carried out at various temperatures (100, 110 and 120◦C)nd times (15, 30 and 60 min), then antioxidant activities of

he HW extracts were evaluated by determining 1,1-diphenyl-2-icrylhydrazyl (DPPH) radical scavenging activity, oxygen radicalbsorbance capacity (ORAC), total phenol content (TPC), and total