usda cereal crops research unit mdi wimadison, wi

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USDA Cereal Crops Research Unit M di WI Madison, WI

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Page 1: USDA Cereal Crops Research Unit Mdi WIMadison, WI

USDA Cereal Crops Research UnitM di WIMadison, WI

Page 2: USDA Cereal Crops Research Unit Mdi WIMadison, WI

USDA Cereal Crops Research UnitM di WIMadison, WI

•1933 New cultivars- improved agronomics,unknown malting quality

Page 3: USDA Cereal Crops Research Unit Mdi WIMadison, WI

USDA Cereal Crops Research UnitM di WIMadison, WI

•1933 New cultivars with improved agronomics but of unknown malting quality

•1938 USDA hired 1st scientist dedicated to malting barley quality research

Page 4: USDA Cereal Crops Research Unit Mdi WIMadison, WI

USDA Cereal Crops Research UnitM di WIMadison, WI

•1933 New cultivars with improved agronomics but of unknown malting quality

•1938 ARS hired 1st scientist dedicated to research on malting barley quality

1948 B l M lt L b t t t d•1948 Barley Malt Laboratory constructed

Page 5: USDA Cereal Crops Research Unit Mdi WIMadison, WI

USDA Cereal Crops Research UnitM di WIMadison, WI

•1933 New cultivars with improved agronomics but of unknown malting quality

•1938 USDA hired 1st scientist dedicated to research on malting barley quality

1948 B l M lt L b t t t d•1948 Barley Malt Laboratory constructed

•1982 Cereal Crops Research Unit established

Page 6: USDA Cereal Crops Research Unit Mdi WIMadison, WI

USDA Cereal Crops Research UnitM di WIMadison, WI

Mission: To identify and characterize biological and chemical mechanisms in barley and oats that affect cereal quality leading to enhanced germplasm

To evaluate barley and oat germplasm for critical quality traits

Page 7: USDA Cereal Crops Research Unit Mdi WIMadison, WI

USDA Cereal Crops Research UnitM di WIMadison, WI

•Improve malting barley quality through developing improvedselection criteria and quality analysis of breeding lines Al Budde, Mark Schmittudde, a Sc tt

•Increasing barley’s genetic resistance to SCAB (Fusarium) Ron Skadsen

•Improving nutritional value of barley and oats Mitch Wise

•Improving efficiency of fermentable sugar production in malting barleyCynthia Henson

Page 8: USDA Cereal Crops Research Unit Mdi WIMadison, WI

Cereal Crops Research Unit

502 Walnut St.502 Walnut St.Madison, WI

Page 9: USDA Cereal Crops Research Unit Mdi WIMadison, WI

The End

Page 10: USDA Cereal Crops Research Unit Mdi WIMadison, WI
Page 11: USDA Cereal Crops Research Unit Mdi WIMadison, WI

Cynthia HensonCynthia Henson

Page 12: USDA Cereal Crops Research Unit Mdi WIMadison, WI

Objectives:Objectives:• Increase fermentable sugar production• Increase fermentable sugar production

by finding or creating better enzymes

• Identify and characterize novel genes encoding carbohydrases and characterize the gene productsc a acte e t e ge e p oducts

• Develop chemical signatures diagnostic of• Develop chemical signatures diagnostic of elite malts with the best brew house

f (hi h t RDF)performance (highest RDF)

Page 13: USDA Cereal Crops Research Unit Mdi WIMadison, WI

Characterizing and Using Genetic Diversity

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622

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313B

MW

G07

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669

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GBR

306

BCD

402B

BCD

351D

MW

G63

5 AG

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G10

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484

MW

G05

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285

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267

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308

ABG

472

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319A

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1551

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288

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366

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397

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319C

Bm

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ASE

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BA

307A

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634

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MW

G07

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669

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306

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MW

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267

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366

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397

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ASE

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634

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MW

G07

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669

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306

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MW

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366

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397

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ASE

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MW

G07

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669

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306

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MW

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1551

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366

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397

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ASE

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MW

G07

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669

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306

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MW

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484

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G05

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1551

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288

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366

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397

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ASE

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MW

G07

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669

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306

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MW

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MW

G05

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267

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1551

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366

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397

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ASE

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MW

G07

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669

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306

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MW

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1551

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366

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397

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ASE

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MW

G07

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306

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MW

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1551

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366

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397

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ASE

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MW

G07

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669

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MW

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366

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397

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ASE

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MW

G07

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MW

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267

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1551

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288

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366

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397

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ASE

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MW

G07

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MW

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397

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ASE

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397

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313B

MW

G07

7C

DO

669

B32E

GBR

306

BCD

402B

BCD

351D

MW

G63

5 AG

BR15

46Tu

bA1

Dhn

6W

G10

26B

Adh

4A

BA

003

ABG

484

MW

G05

8A

BC32

1K

FP19

5W

G46

4bB

E54A

BCD

453B

GBR

285

GBR

267

GBR

308

ABG

472

ABG

319A

GBR

1551

iHxk

2A

BG50

0BbA

P91

GBR

288

ABG

366

ABG

397

ABG

319C

Bm

y1ks

uH11

ASE

1CA

BA

307A

GB

R19

5M

WG

634

WG

622

ABG

313B

MW

G07

7C

DO

669

B32E

GBR

306

BCD

402B

BCD

351D

MW

G63

5 AG

BR15

46Tu

bA1

Dhn

6W

G10

26B

Adh

4A

BA

003

ABG

484

MW

G05

8A

BC32

1K

FP19

5W

G46

4bB

E54A

BCD

453B

GBR

285

GBR

267

GBR

308

ABG

472

ABG

319A

GBR

1551

iHxk

2A

BG50

0BbA

P91

GBR

288

ABG

366

ABG

397

ABG

319C

Bm

y1ks

uH11

ASE

1CA

BA

307A

GB

R19

5M

WG

634

WG

622

ABG

313B

MW

G07

7C

DO

669

B32E

GBR

306

BCD

402B

BCD

351D

MW

G63

5 AG

BR15

46Tu

bA1

Dhn

6W

G10

26B

Adh

4A

BA

003

ABG

484

MW

G05

8A

BC32

1K

FP19

5W

G46

4bB

E54A

BCD

453B

GBR

285

GBR

267

GBR

308

ABG

472

ABG

319A

GBR

1551

iHxk

2A

BG50

0BbA

P91

GBR

288

ABG

366

ABG

397

ABG

319C

Bm

y1ks

uH11

ASE

1CA

BA

307A

cM

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3cM

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

5.1

1.4

10.6 2.1

3.1

7.7

1.4

7.3

3.3

4.0

1.8

6.3

4.7

2.0

3.1

2.3

2.0

2.0

2.7

3.3

4.0

3.3

11.1 2.7

3.0

0.0

0.8

4.9

8.9

5.0

2.7

2.7

0.0

3.3

3.3

13.7

18.1 1.6

5.5

9.3

343Steptoe CGGGACGTCGGCACGCGTGATCCCGACATTTTCMore CGGGACGTCGGCACGTGTGATCCCGACATTTTC

Bmy1 DNA sequence, amino acid sequence and enzyme effectiveness

Morex CGGGACGTCGGCACGTGTGATCCCGACATTTTC

115Steptoe RDVGTRDPDIF Morex RDVGTCDPDIF

+67%Steptoe *********************************Morex **********

ff ienzyme effectiveness

Page 14: USDA Cereal Crops Research Unit Mdi WIMadison, WI

Metabolic Profiling of Malting BarleyGC MS and Multivariate AnalysisGC-MS and Multivariate Analysis

Page 15: USDA Cereal Crops Research Unit Mdi WIMadison, WI

Metabolic Profiling of Malting BarleyGC MS and Multivariate AnalysisGC-MS and Multivariate Analysis

Page 16: USDA Cereal Crops Research Unit Mdi WIMadison, WI

Mitch WiseMitch Wise

Research Chemist, hired in January 2002, to work on improvingnutritional aspects of oat and barley p y

Served in Baghdad since January 2004

Page 17: USDA Cereal Crops Research Unit Mdi WIMadison, WI

Cutting Edge Researchg g

• Studying signaling pathways that elicit biosynthesis of avenanthramides in oat

• Large scale synthesis of avenanthramides for studying the metabolism of and the nutritional aspects of thesemetabolism of and the nutritional aspects of these antioxidants, specifically their roles in reducing stress related oxidative damage and the development of atherosclerosis

Page 18: USDA Cereal Crops Research Unit Mdi WIMadison, WI

USDA Cereal Crops Research Unit