U.S. NSF’s Assembling the Tree of Life: An IntegrativeApproach to Investigating Cnidarian Phylogeny

Cnid ToL: 2006-2010

University of KansasPaulyn Cartwright, Daphne Fautin & Allen Collins(Collins: National Systematics Lab of NOAA’s Fisheries Service& Smithsonian’s National Museum of Natural History)

Ohio State UniversityMeg Daly

Harvey Mudd CollegeCathy McFadden

Duke UniversityCliff Cunningham

Northern Illinois UniversityNeil Blackstone

University of the Virgin IslandsSandra Romano

Pomona CollegeDaniel Martinez

9 PIs

Mike Dawson (University of California, Davis)Scott France (University of Louisiana at Lafayette)Steven Haddock (Monterey Bay Aquarium Research Institute)Dan Janies (Ohio State University)Antonio Carlos Marques (Universidade de Sao Paulo)Peter Schuchert (Muséum d‘Histoire Naturelle de Genève)

Phil Alderslade (Australia), Steven L. Bailey (USA), Bastian Bentlage (Germany), Nando Boero (Italy), Steve Cairns (USA), Dale Calder (Canada), Casey Dunn (USA), Yakko Hirano (Japan), Thomas Jankowski (Switzerland), B. P. Jupp (Oman), Shin Kubota (Japan), Nancy Knowlton (USA), Cheryl Lewis Ames (USA), Alberto Lindner (Brazil), Dhugal Lindsay (Japan), George Mackie (Canada), George Matsumoto (USA), Mónica Medina (USA), Hermes Mianzan (Argentina), Alvaro Migotto (Brazil), Maria Pia Miglietta (Panama), Tina Molodtsova (Russia), Zilda Braga Morais (Portugal), Andre Morandini (Brazil), Gustav Paulay (USA), Vicki and John Pearse (USA), Bernard Picton (UK), Phil Pugh (UK), Ekaterina Raikova (Russia), Kevin Raskoff (USA), Bernd Schierwater (Germany). . .And Many More!

Dozens of Collaborators

Some Diversity of Medusozoa

Stauromedusae Cubomedusae CoronataeSemaeostomeae

Rhizostomeae

Trachymedusae

Narcomedusae

LimnomedusaeActinulida

LaingiomedusaeLeptothecata Anthoathecata

Siphonophora

Actiniaria Ceriantharia Antipatharia

Zoanthidea Corallimorpharia Scleractinia

Some Diversity of Hexacorallia

O. Pennatulacea

Sea pens

O. Alcyonacea O. Helioporacea

Blue coral

Soft corals & sea fans 14 families

2 families

29 familiesPhotos: G.C. Williams

Some Diversity of Octocorallia

Anthozoa

Staurozoa

Cubozoa

HydrozoaScyphozoaOctocoralli

aHexacorallia

Medusozoa

10,000 – 11,000 Accepted Species

mt

mt

Bridge et al., 1995

Obtain DNA from 1,800+ cnidarian species from around the world

Collection/vouchering standards are BarCode compliant, documented through new module of “Hexacorallians of the World”

Cover every “family” and focus on key clades of interest

Generate 23 million bp of sequence data, from 10 or so markers

Reconstruct and test hypotheses of evolutionary relationships

What We Promised To Do

Integration CMoL, CBOL, etc.

Positive Externality for Barcoding: CO1 sequences, BOLD?

Will ID and sequence CO1 (and 16S) from any medusozoan species and disparate anthozoans (95% eth, but medusae need second/portion in 4% buff. Formalin), but. . . .

Validation: CO1 and taxonomic identifications, genetic IDs

Will provide multiples to those interested in assessing CO1 variation

Will work on species-level marker(s) for Anthozoa

I will barcode and try to ID any glass sponges (Hexactinellida)