TECHNICAL SERVICE REPORT NO. 91809

ULTRA COSMETICS January 2010

Client: Test Laboratory: ULTRA COSMETICS Technical Service Laboratory Thor Specialties via Puccini,30 I-21010 Besnate (VA) - Italy Contact Katia Padoan Telephone +39 0331 279326 Telefax +39 0331 279350 Email k.padoan@thoritaly.it

TECHNICAL SERVICE REPORT N° 91809

ULTRA COSMETICS

Pag 3

Test results

1. Lotion containing 1% Acticide DDQ and 5% Isopropyl Alcohol

Test procedure (Test concentration/contact time)

Test organisms

Bacterial test

suspension N

90%

1 minute

Staphilococcus aureus

5.0 x 108

Na R

< 1.5 x 102

≥105

Pseudomonas aeruginosa

5.0 x 108

Na R

1.8 x 102

≥105

Escherichia coli K12 4.8 x 108

Na R

< 1.5 x 102

≥105

Enterococcus hirae 4.8 x 108

Na R

< 1.5 x 102

≥105

TECHNICAL SERVICE REPORT N° 91809

ULTRA COSMETICS

Pag 4

Test method EN 1650:2008 Quantitative suspension test for the evaluation of fungicide activity of chemicals disinfectants and antiseptics used in food, industrial, domestic, and institutional areas.

Definitions Fungicidal activity (EN 1650) Capability of the product to produce at least a 10

4 reduction in the number of viable fungi cells

belonging to reference strains of Candida albicans and Aspergillus niger under conditions defined by this European Standard (15 minutes, 20°C). Clean conditions Conditions representative of surfaces which have received a satisfactory cleaning programme and/or are known to contain minimal levels of organic and/or inorganic materials.

Principle A test suspension of fungi and yeast in a solution of interfering substances is added to a prepared sample of the product under test diluted in hard water. The mixture is maintained at 20°C for 15 minutes (required test conditions). At this contact time, an aliquot is taken; the fungicidal and/or fungostatic action in this portion is immediately neutralized or suppressed by a validated method. The method of choice is dilution-neutralization. The number of surviving bacteria in each sample are determined and the reduction in viable counts is calculated.

Experimental condition Product test concentration: undiluted (80% is the highest assayable concentration) Contact time: 15 minutes Test temperature: 20°C Neutraliser: Leethen broth modified (Tween 80 30 g/l, Lecitine 3 g/l, Saponine 30 g/l) Interfering substance: 0.3 g/l of bovine albumin Temperature of incubation: 30°C Identification of the microbial strains used : Candida albicans ATCC 10231 Aspergillus niger ATCC 16404

TECHNICAL SERVICE REPORT N° 91809

ULTRA COSMETICS

Pag 5

Test results

1. Lotion containing 1% Acticide DDQ and 5% Isopropyl Alcohol

Test procedure (Test concentration/contact time)

Test organisms

Microbial test

suspension N

80%

15 minutes

Candida albicans 3.3 x 107

Na R

< 1.5 x 102

≥104

Aspergillus niger 1.6 x 107

Na R

< 1.5 x 102

≥104

TECHNICAL SERVICE REPORT N° 91809

ULTRA COSMETICS

Pag 6

Calculation

For the test procedure where the number of cfu on all plate counted is lower than 15, record the viable count of the test mixture as lower than 150. Instead where the number of cfu on all plate counted is higher than 300, record the viable count of the test mixture as higher than 3000

Na = c___ n x d x V where: c = sum of the colonies counted on both plates; n = number of plates taken into account; d = dilution factor corresponding to the dilution taken into account; V = sample volume.

R = reduction in viability = N x 10-1

Na Where: N = number of cfu/ml of the bacterial test suspension Na = number of cfu/ml of the test mixture Even if in the European standard EN 1650 (for fungicide activity) the results are expressed in logarithmic form, in this report the results are presented in exponential form in order to achieve more uniformity with bactericidal activity evaluation (EN 1276). 25.01.2010 Technical Manager Katia Padoan Please note that unless otherwise stated, the conclusions and any recommendations, either made or implied, are based on information drawn from examination of the samples identified in this report only. Since these may be influenced by, for example, infection level variations in raw materials, stored component solutions and manufacturing equipment, it is recommended that some appropriate monitoring of microbiological properties be carried out.

THOR SPECIALTIES ⋅ Via Puccini 30 ⋅ I-21010 Besnate (VA) ⋅ ITALY

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