ultimate 3000 x2 system capillary...

UltiMate 3000 x2 System Capillary Kits Quick Installation Guide

26/10/2007 Rev. 3.1 M4823.0050 © 2007 Dionex

AnutaNew Stamp

Table of Contents 1. Introduction ...................................................................................................................... 1

1.1 General System Preparation ....................................................................................... 1 1.2 Connecting the Eluent Line to the Solvent Reservoir ................................................ 1 1.3 When to use Which Connector................................................................................... 3 1.4 Using 10-Port Instead of 6-Port TCC-3x00 Valves ................................................... 5

2. Tandem Operation Capillary Kits, Dual-Ternary Analytical (P/N 6037.0001) or Micro (P/N 6037.0002) ............................................................................................................. 6

2.1 Included in Delivery................................................................................................... 7 2.2 Stacking the System Components .............................................................................. 8 2.3 Flow Schematic .......................................................................................................... 9 2.4 Connecting the SRD-3600 to the DGP-3600A or M ................................................. 9 2.5 Connecting the Left Pump to the TCC-3100 ............................................................. 9 2.6 Connecting the Right Pump to the WPS-3000SL .................................................... 11 2.7 Installing Capillaries within the TCC-3100 ............................................................. 12 2.8 Connecting the Detector........................................................................................... 13

3. Application Switching Kits, Dual-Ternary Analytical (P/N 6037.0009) or Micro (P/N 6037.0010)....................................................................................................................... 15

3.1 Included in Delivery................................................................................................. 16 3.2 Stacking the System Components ............................................................................ 17 3.3 Flow Schematics ...................................................................................................... 18 3.4 Connecting the SRD-3600 to the DGP-3600A ........................................................ 19 3.5 Connecting the Columns within the TCC-3200....................................................... 19 3.6 Connecting the Pumps.............................................................................................. 21

4. On-line Sample Preparation Capillary Kits, Dual-Ternary Analytical (P/N 6037.0006) or Micro (P/N 6037.0007) ................................................................................... 23

4.1 Included in Delivery................................................................................................. 24 4.2 Stacking the System Components ............................................................................ 25 4.3 Flow Schematics ...................................................................................................... 26 4.4 Connecting the SRD-3600 to the DGP-3600A or M ............................................... 26 4.5 Connecting the WPS-3000SL .................................................................................. 27 4.6 Connecting the Analytical or Micro Column........................................................... 27

4.6.1 Setup with PDA-3000 ...................................................................................... 28 4.6.2 Setup with VWD-3100/VWD-3400................................................................. 29

4.7 Installing the SPE Column ....................................................................................... 30 5. Parallel Operation Capillary Kits, Dual-Ternary Analytical (P/N 6037.0004) or Micro (P/N 6037.0005) ........................................................................................................... 31

5.1 Included in Delivery................................................................................................. 32 5.2 Stacking the System Components ............................................................................ 33 5.3 Flow Schematic ........................................................................................................ 34 5.4 Connecting the SRD-3600 to the DGP-3600A or M ............................................... 34 5.5 Connecting the Pumps to the TCC-3100 Switching Valve...................................... 35 5.6 Connecting the Analytical or Micro Column and Sampler...................................... 36 5.7 Connecting the Detectors ......................................................................................... 37

6. 2D-LC (Affinity-Reversed Phase, e.g., IMAC-C18) Kit, Dual-Ternary Analytical (P/N 6037.0008)....................................................................................................................... 38

6.1 Included in Delivery................................................................................................. 39

October 2007 Rev. 3.1 Page i © 2007 Dionex

6.2 Stacking the System Components ............................................................................ 40 6.3 Flow Schematic ........................................................................................................ 41 6.4 Connecting the SRD-3600 to the DGP-3600A ........................................................ 41 6.5 Connecting the WPS-3000SL .................................................................................. 42 6.6 Connecting the Tubings within the TCC-3100 ........................................................ 43

7. Tandem Operation Capillary Kits, Dual-Binary Analytical (P/N 6037.0013) or Micro (P/N 6037.0003) ........................................................................................................... 47

7.1 Included in Delivery................................................................................................. 48 7.2 Stacking the System Components ............................................................................ 49 7.3 Flow Schematic ........................................................................................................ 50 7.4 Connecting the SRD-3x00 to the HPG-3x00A or M ............................................... 50 7.5 Installing the Columns in the TCC-3100 ................................................................. 51 7.6 Further Connections within the TCC-3100.............................................................. 52 7.7 Connecting the Detector........................................................................................... 54

8. Application Switching Kits, Dual-Binary Analytical (P/N 6037.0011) or Micro (P/N 6037.0012)................................................................................................................................ 55

8.1 Included in Delivery................................................................................................. 56 8.2 Stacking the System Components ............................................................................ 57 8.3 Flow Schematics ...................................................................................................... 58 8.4 Connecting the SRD-3x00 to the HPG-3x00A or M ............................................... 59 8.5 Connecting Both Pumps to the Right TCC Switching Valve .................................. 60 8.6 Installing Capillaries within the TCC....................................................................... 60 8.7 Connecting the Autosampler .................................................................................... 62 8.8 Installing the Detector’s Waste Capillary ................................................................ 63

9. Automated Method Scouting, Quaternary Analytical (P/N 6722.0101) or Micro (P/N 6722.0100)....................................................................................................................... 64

9.1 Included in Delivery................................................................................................. 65 9.2 Stacking the System Components ............................................................................ 68 9.3 Flow Schematic ........................................................................................................ 69 9.4 Connecting the LPG-3400A or M Pump to the WPS-3000SL Sampler .................. 70 9.5 Connecting the WPS-3000SL to the Column Compartment ................................... 71 9.6 Connecting the Analytical Columns in the Column Compartment.......................... 72 9.7 Connecting the Column Compartment to the Detector............................................ 75

Page ii Rev. 3.1 October 2007 © 2007 Dionex

1. Introduction

1.1 General System Preparation Allow the system modules to warm up to a moderate temperature for 4 hours to allow any condensation that might have occurred during transit to evaporate. Do not connect the units to the mains during this period. If condensation has not completely evaporated after 4 hours, allow the system to continue to warm up (without connecting it to the mains) until the condensation is completely gone. Place the system on a firm and level surface that is free of vibration. Make sure that the surface is resistant to solvents. Avoid locations with extreme changes in temperature (such as, direct sunlight or drafts) and high air humidity.

Note: For information about the power and communication connections (LAN, USB) as well as the installation procedures for other components, such as the Active Rear-Seal Wash System, please refer to the operating instructions for the system components.

1.2 Connecting the Eluent Line to the Solvent Reservoir 1. Feed the eluent line through the retaining guide and then into the opening in the reservoir

cap.

2. Slide the filter holder with filter frit onto the end of the eluent line. Verify that the end of the eluent line is cut straight and is not deformed. If necessary, cut the tubing with a sharp knife. Use the eluent lines shipped with the solvent rack only.

3. Place the complete assembly in the solvent reservoir.

4. Tighten the reservoir cap hand-tight by holding the cap and turning the bottle.

October 2007 Rev. 3.1 Page 1 © 2007 Dionex

Retaining guide

Pa

Filter holder with filter frit

Note: To exchange the tubes, first remove the frit, and then remove the eluent tube before removing the retaining guide.

Important: Use eluent tubes with filter frits only for drawing eluents from the eluent bottles. This prevents contamination of the HPLC system.

Note: Regularly check the frits for permeability. Especially when working with aqueous solvents, algae and other microorganisms can grow on the frits and clog them. Therefore, exchange the solvents at regular intervals. Rinse the bottles thoroughly before using them again. Replace the frits as necessary.

ge 2 Rev. 3.1 October 2007 © 2007 Dionex

1.3 When to use Which Connector

Different types of connectors are used for connecting the various tubing in the system. Depending on the type of tubing, stainless steel or PEEK, the connector is chosen accordingly. Always use Rheodyne fittings on the Rheodyne valves, which are installed in the TCC-3x00 and the WPS-3000SL. For all other connections use the fingertight (PEEK tubing) or the nut and ferrule (stainless steel tubing) depicted on the next page.

Rheodyne stainless steel nut and ferrule to be used on the valves of the TCC-3x00 and injection valve of the WPS-3000SL in combination with stainless steel tubing

Rheodyne PEEK nut and ferrule to be used on the valves of the TCC-3x00 and injection valve of the WPS-3000SL used with PEEK tubing.


Stainless steel nut and ferrule to be used on all other then Rheodyne stainless steel connections unless otherwise especially noted.

PEEK fingertight to be used on all other then Rheodyne PEEK connections unless otherwise especially noted.

Page 4 Rev. 3.1 October 2007 © 2007 Dionex

1.4 Using 10-Port Instead of 6-Port TCC-3x00 Valves The installation steps for the Application Switching, Parallel Operation and On-line Sample Preparation Capillary Kits in this installation manual refer to Thermostatted Column Compartments with 2-position 6-port switching valves. However, TCC-3x00s with 2-position 10-port switching valves can also be used for these applications. Compared to the 6-port valve setup, capillaries are connected to identical port numbers of these 10-port valves as shown below.

The 6-port valve has a direct fluidic connection between port 1 and port 6. To achieve this fluidic connection for the 10-port valve, Dionex recommends installing an additional capillary between port 7 and port 10. The inner diameter of the capillary should be 0.13 mm (0.005″) for a micro setup or 0.25 mm (0.01″) for an analytical setup. Port 8 and port 9 are not in use and not plumbed. To avoid false leak alarms, these ports should be sealed with a plug or connected by an additional capillary.

Important: The capillary needed to connect port 7 with port 10 and the plugs or additional capillary for ports 8 and 9 are NOT included in delivery.

Please note that all capillary labels and descriptions within this guide are valid also for this 10-port setup. However, in some scenarios it might be beneficial to use a different approach.


2. Tandem Operation Capillary Kits, Dual-Ternary Analytical (P/N 6037.0001) or Micro (P/N 6037.0002)

Quick Installation Guide


This Quick Installation Guide explains the basic installation steps for an analytical or micro UltiMate 3000 Dual-Ternary HPLC System for dual-column tandem mode with off-line column regeneration. The procedure how to install the application kit is identical for both the analytical and micro version. The only difference between the application kits is the inner diameter of the capillaries, i.e., 0.25 mm ID for the analytical version and 0.18 mm ID (from pump outlet) and 0.12 mm ID (all other connections) for the micro version. For additional information, please refer to the operating instructions of the modules.

2.1 Included in Delivery

Description Qty

Capillary Left Pump - TCC port 8 1

Capillary TCC 6p / port 2 l. or 10p / port 7 - Col. 2 / Col. 1 (5 cm) 1

Capillary TCC 6p / port 4 r. or 10p / port 5 - Col. 2 (5 cm) 1





Capillary TCC port 10 - Col. 1 1


Capillary TCC port 4 - TCC port 9 1

Capillary TCC 6p /port 2 r. or 10p /port 1 - Det. 1

PEEK capillary for VWD waste connection 1

Capillary holder, self-adhesive, PVC 1

Finger-tight 33 mm fitting set, 5 pcs 1

Quick Installation Guide U3000 Application Kits 1


2.2 Stacking the System Components

Please stack the components as shown in the image on the left: SRD-3600 Solvent Rack and Degasser DGP-3600A Dual-Gradient Analytical Pump or DGP-3600M Dual-Gradient Micro Pump WPS-3000SL Analytical or Micro In-Line Split Loop Autosampler TCC-3100 1x2P-10P Thermostatted Column Compartment with 1x 2-Position 10-Port Switching Valve PDA-3000 Photodiode Array Detector or VWD-3x00 Variable Wavelength Detector

Important: The modules are primed with 2-propanol. During initial operation of the

system, make sure that the solvents used are miscible with 2-propanol. Otherwise, follow the appropriate intermediate steps.


2.3 Flow Schematic The figure below shows the principle setup of a dual-column tandem mode configuration. For more detailed information, please refer to the Chromeleon online help.

2.4 Connecting the SRD-3600 to the DGP-3600A or M The SRD-3600 Solvent Rack is shipped with six 1-liter eluent bottles, appropriate tubing, and bottle caps. Please refer to the Operating Instructions of the solvent rack for details about how to connect the eluent bottles to the solvent rack and the solvent rack to the pump inlet.

2.5 Connecting the Left Pump to the TCC-3100 Open the front panels of all modules.

The DGP-3600A/M Dual Low-Pressure Gradient Pump contains two ternary pumps. For further information about how to install the pump, please refer to the Operating Instructions.

Outlet Left Pump

Outlet Right Pump

Left Pump Right Pump


Insert the capillary named “Left pump - TCC port 8” by tilting it behind the WPS front panel.

Guide the capillary through the left opening in the pump base.

Mount the capillary using the clamp on the left side of the system tower..

Connect the capillary to port 8 of the 10-port switching valve in the TCC.

Port 8


2.6 Connecting the Right Pump to the WPS-3000SL

The WPS is shipped with a capillary to connect the pump to the WPS. Guide the capillary through the right opening in the base and connect it to the right pump outlet. For further information, please refer to the WPS Operating Instructions.

Connect the other end of this capillary to the inlet of the WPS injection valve (port 5).

The WPS ship kit includes a capillary for connecting the WPS to the TCC. After guiding the capillary through the WPS’s outlet to the column oven, connect the capillary to the outlet of the WPS (port 4) and to port 6 of the TCC valve.

Outlet Left Pump

Outlet Right Pump


Port 4 Port 5

Port 6

Port 4


2.7 Installing Capillaries within the TCC-3100

Note: We highly recommend the use of column ID chip cards (P/N 6710.1505, set of 5 ID chip cards). This allows for easy tracking the column used for each sample. The ID chip cards are not shown in the images below.

Install two identical columns with the flow direction as indicated in the figure above. Below, the upper column is referred to as “column 1”, the other column as “column 2”.

Use the capillary labeled “TCC1” to connect the outlet of colu10 of the TCC valve. Then, use the capillary labeledCol. 2” to connect the outlet oport 2 of the TCC valve.

Use the capillary labeled “TCC 6p / port 2 l. or 10p / port 7 - Col. 2 / Col. 1” to connect the inlet of column 1 to port 7. Use the capillary labeled “TCC 6p / port 4 r. or 10p / port 5 - Col. 2” to connect the inlet of column 2 to port 5.

Install the capillary labeled “TCTCC port 9” between port 9 athe TCC valve.

Column 2

Column 1 0

Inlet column 1 to port 7


Page 12 Rev. 3.1 © 2007 Dionex

Port 1

port 10 - Col. mn 1 to port

“TCC port 2 - f column 2 to

C port 4 - nd port 4 of

Port 2

Port 4

Port 9

October 2007

Install the PEEK capillary provided in the application kit to connect port 3 to the waste. In addition to the depicted SST nut, an optional PEEK fitting is provided with the kit.

Port 3

2.8 Connecting the Detector The Tandem Operation Capillary Kits, Dual-Ternary Analytical or Micro support both the PDA-3000 Photodiode Array Detector and the VWD-3x00 Variable Wavelength Detector. The following images refer to the VWD-3x00.

Use the capillary labeled “TCC 6p / port 2 r. or 10p / port 1 - Det.” to connect port 1 to …

…the inlet of the VWD’s flow cell. To connect the PDA-3000, please use the capillary from the detector’s ship kit.

Port 1

Flow cell inlet


Install a PEEK capillary in the outlet of the flow cell and connect it to the waste (capillary not included in the application kit).

Make sure to guide the capillaries connected to the flow cell through the detector’s capillary slits as depicted above. Proceed accordingly when connecting the PDA-3000.

Capillary slits

Flow cell inlet

Well done, you have successfully installed the Tandem Operation Capillary Kit, Dual-Ternary Analytical or Micro.


3. Application Switching Kits, Dual-Ternary Analytical (P/N 6037.0009) or Micro (P/N 6037.0010)



This Quick Installation Guide explains the basic installation steps for an analytical or micro UltiMate 3000 Dual-Ternary HPLC System for application switching. The procedure how to install the application kit is identical for both the analytical and the micro version. The only difference between the kits is the inner diameter of the capillaries, i.e., 0.25 mm ID for the analytical version and 0.18 mm ID (from pump outlet) and 0.12 mm ID (all other connections) for the micro version. For additional information, please refer to the Operating Instructions of the modules.


Description Qty




Capillary TCC 6p / port 2 r. or 10p / port 1 - Det. 1

Capillary TCC port 1 r. - Col. 1 (15 cm, 25 cm) 1


Capillary Left Pump - TCC port 2 l. 1

Capillary Right Pump - TCC port 6 l. 1

Capillary Col. 1 (5 cm) - TCC port 5 l. 1

Capillary Col. 1 (15 cm, 25 cm) - TCC port 5 l. 1

Capillary TCC port 6 r. - TCC port 4 l. 1

Capillary WPS port 5 - TCC port 1 l. 1







Please stack the components as shown in the image on the left: SRD-3600 Solvent Rack and Degasser DGP-3600A Dual-Gradient Analytical Pump or DGP-3600M Dual-Gradient Micro Pump WPS-3000SL Analytical or Micro In-Line Split Loop Autosampler TCC-3200 2x2P-6P Thermostatted Column Compartment with 2x 2-Position 6-Port Switching Valves PDA-3000 Photodiode Array Detector or VWD-3x00 Variable Wavelength Detector




3.3 Flow Schematics The figure below shows the principle of automated application switching. After termination of the first application (“analyze on column # 1”), valve 1 is switched to purge the autosampler with the eluents which will be used for the second application (“wash autosampler”). Then valve 2 is switched, allowing column equilibration and analyses on column 2 to be started (“analyze on column # 2”).

Note: For reasons of clarity, the port positions of valve 1 and 2 are shown clockwise

and counterclockwise. In the real system setup, both valves use clockwise numbering.


3.4 Connecting the SRD-3600 to the DGP-3600A The SRD-3600 Solvent Rack is shipped with six 1-liter eluent bottles, appropriate tubing, and bottle caps. Please refer to the Operating Instructions of the solvent rack for details on how to connect the eluent bottles to the solvent rack and the solvent rack to the pump inlet.

3.5 Connecting the Columns within the TCC-3200 Open the front panels of all modules.

Note: Dionex strongly recommends using column ID chip cards (P/N 6710.1505, set of 5 ID chip cards). This allows for easy tracking the column used for each sample.

Place column 1 on the open TCC cover with the orientation of the flow direction as indicated in the image. After attaching the column ID chip card, connect a capillary labeled “Col. 1 - TCC port 5 l." at the left and a capillary labeled “TCC port 1 r. – Col. 1” at the right end. The application kit includes optimized capillary lengths for connecting different column lengths.

Place the column in the column oven and insert the ID chip card in an ID-slot. Connect the left capillary to port 5 and the right capillary to port 1 of the respective switching valves.

Port 1 Port 5


Mount column 1 by clicking the rings (clamp counter part) onto the clamps. Then insert column 2 with the indicated flow orientation and insert its ID chip card in a slot. Use the capillary labeled “TCC port 3 r. – Col. 2” to connect the column’s outlet to port 3 of the right switching valve.

Uorcoco(c

Use the capillary labeled “TCC 6p/port 2r. or 10p/port 1 – Det” to connect port 2 of the right valve to the inlet of the VWD-3x00 flow cell. Guide the capillary through the VWD’s capillary slit. For connecting the PDA-3000, please use the capillary from the detector’s ship kit.

Inflonotocedeco

Port 2 Capillary slit

t

Page 20 Rev. 3. © 2007 Dio

Inlet column 2

se the capillary labeled “TCC 6p/ 10p/port 5 Col. 2” to connect thlumn 2 with port 4 of the right vlumn 2 in the clamps by clicking lamp counter part) onto the clamp

stall a PEEK capillary in the outlw cell and connect it to the wastet included in the application kit). guide the capillaries connected toll through the detector’s capillarypicted above. Proceed accordinglnnecting the PDA-3000.

Capillary slits

1 Ocnex

Port 4

port 4 r. e inlet of alve. Fix the rings s.

Flow cell inle

et of the (capillary Make sure the flow slits as y when

tober 2007

Insert the capillary labeled “TCC port 6 r. - TCC port 4 l.” between the left valve (port 4) and the right valve (port 6).

Use port 3 of the left valve to connect the PEEK waste capillary.

Port 4 Port 3 Port 6

3.6 Connecting the Pumps

Use the capillary labeled “Right pump - TCC port 6 l.” to connect the right pump outlet to the port 6 of the left TCC valve. Guide the capillary through the opening in the bottom of the pump.

To connect the left pump, use the capillary labeled “Left pump - TCC port 2 l.” to connect the outlet of the left pump to port 2 of the left TCC valve.


Guide the capillary labeled “WPS port 5- TCC port 1 l.” through the WPS outlet to the column oven (also see the OI for details). Then, connect port 5 of the WPS injection valve to port 1 of the left TCC valve.

Finally, use the capillary shipped with the WPS to connect port 4 of the WPS injection valve to port 5 of the right TCC valve (not shown). Well done, you have successfully installed the Application Switching Kit, Dual-Ternary Analytical or Micro.


4. On-line Sample Preparation Capillary Kits, Dual-Ternary Analytical (P/N 6037.0006) or Micro (P/N 6037.0007)



This Quick Installation Guide explains the basic installation steps for an analytical or micro UltiMate 3000 Dual-Ternary HPLC System for on-line sample preparation. The procedure how to install the application kit is identical for both the analytical and the micro version. The only difference between the kits is the inner diameter of the capillaries, i.e., 0.25 mm ID for the analytical version and 0.18 mm ID (from pump outlet) and 0.12 mm ID (all other connections) for the micro version. For additional information, please refer to the Operating Instructions of the modules.


Description Qty

Capillary Left Pump - TCC port2 1

Capillary TCC port 1 - Col. (5 cm) VWD use 1



Capillary TCC port 1 - Col. (5 cm) PDA use 1



Capillary TCC port 3 - SPE Outlet 1

Capillary TCC port 6 - SPE Inlet 1




Column Clips Kit 1




Please stack the components as shown in the image on the left: SRD-3600 Solvent Rack and Degasser DGP-3600A Dual-Gradient Analytical Pump or DGP-3600M Dual-Gradient Micro Pump WPS-3000SL Analytical or Micro In-Line Split Loop Autosampler TCC-3100 1x2P-6P Thermostatted Column Compartment with 1x 2-Position 6-Port Switching Valve PDA-3000 Photodiode Array Detector or VWD-3x00 Variable Wavelength Detector



October 2007 Rev. 3.1 Page 25 © 2007Dionex

4.3 Flow Schematics The figure below shows a typical setup of an On-line SPE-LC system with a 2-position 6-port switching valve. In the sample fractionation step, the analytes are trapped on an SPE column while the matrix is flushed to waste. In the second step, the analytes are transferred to the analytical column. For further information, please refer to the Chromeleon online help.

Note: The flow scheme above is a theoretical representation. In the real system setup, the left pump is connected to the switching valve while the right pump is connected to the autosampler.



4.5 Connecting the WPS-3000SL

The WPS is shipped with a capillary to connect the pump to the WPS. Guide the capillary through the right opening in the bottom and connect it to the right pump outlet. For further information, please refer to the WPS Operation Instructions.


Port 4 Port 5

The WPS ship kit includes a capillary for connecting the WPS to the TCC. Connect the capillary to the outlet of the WPS (port 4) and to port 5 of the TCC valve (not shown).

4.6 Connecting the Analytical or Micro Column

Note: Dionex strongly recommends using column ID chip cards (P/N 6710.1505, set of 5 ID chip cards). This allows for easy tracking of the separation column and the sample preparation column used for an analysis.

The flow cell is located in a different place for the VWD-3x00 and the PDA-3000. In the VWD-3x00, the cell is located on the left, in the PDA-3000, the cell is located on the right. To ensure the shortest capillary connections between the column outlet and the detector, the way the separation column is connected in the TCC varies depending on the detector type:


4.6.1 Setup with PDA-3000

Place the separation column in the TCC with the orientation of the flow direction as indicated in the image. After attaching the column ID chip card, connect a capillary labeled “TCC port 1 - Col. (PDA)” between the column inlet and port 1 of the TCC valve.

Separation column

Port 1

For connecting the column outlet to the PDA-3000 flow cell, please use the capillary from the detector’s ship kit.

Note: All other capillary connections are the same as in the setup with a VWD-3x00. Therefore, please refer to the VWD-3x00 section below for further installation steps.


4.6.2 Setup with VWD-3100/VWD-3400

Place the separation column in the TCC with the orientation of the flow direction as indicated in the image. After attaching the column ID chip card, connect the capillary labeled “TCC port 1 - Col. (VWD)" between column inlet and port 1 of the TCC valve.

A capillary for cocolumn outlet is pkit. Use this capilthe column to the

Use port 4 of the TCC valve to connect a waste capillary. Install a PEEK capillary in the outlet of the flow cell and connect it to the waste (capillary not included in the application kit).

Make sure to guidto the flow cell thcapillary slits as daccordingly when

Separation column

Flow cell outlet

Port 4

Port 1

Capillary sl

October 2007 Rev. 3.1 © 2007Dionex

Outlet column

t
Flow cell inle
nnecting the VWD to the rovided in the VWD ship lary to connect the outlet of inlet of the flow cell.

e the capillaries connected rough the detector’s epicted above. Proceed connecting the PDA-3000.

its

Page 29

4.7 Installing the SPE Column

Place the on-line SPE column in the TCC with the flow direction as indicated in the image. After attaching the column ID chip card, use the capillary labeled “TCC port 6 - SPE Inlet" to connect port 6 to SPE inlet and the capillary labeled “TCC port 3 - SPE Outlet” to connect the SPE outlet to port 3 of the TCC valve.

Use the capillary labeled “Left pump - TCC port 2” to connect the left pump outlet to the port 2 of the TCC valve. Guide the capillary through the opening in the bottom of the pump.

Mount the capillary with the clamp located on the left side of the system tower.

Port 3

Well done, you have successfully installed the On-line Sample Preparation Capillary Kit, Dual-Ternary Analytical or Micro.


5. Parallel Operation Capillary Kits, Dual-Ternary Analytical (P/N 6037.0004) or Micro (P/N 6037.0005)



This Quick Installation Guide explains the basic installation steps for an analytical or micro UltiMate 3000 Dual-Ternary HPLC System for parallel operation. The procedure how to install the application kit is identical for the analytical and micro versions. The only difference between the kits is the inner diameter of the capillaries, i.e., 0.25 mm ID for the analytical version and 0.18 mm ID (from pump outlet) and 0.12 mm ID (all other connections) for the micro version. For additional information, please refer to the operating instructions of the modules.


Description Qty

Capillary TCC port 6 - Pump left 1

Capillary TCC port 4 - Pump right 1

Capillary TCC port 1 r. - Col. 1 (5 cm) 1

Capillary TCC port 3 r. - Col. 2 (5 cm) 1



Capillary Col. 1 (25 cm) - VWD 1 1

Capillary Col. 2 (25 cm) - VWD 2 1

Capillary TCC port 5 - WPS port 5 1


Capillary Col. 1 (25 cm)- PDA 1 1

Capillary Col. 2 (25 cm)- PDA 2 1


Column Clips Kit 1



5.2 Stacking the System Components Please stack the components as shown in the image: SRD-3600 Solvent Rack and Degasser DGP-3600A/M Dual-Gradient Analytical/ Micro Pump WPS-3000SL Analytical or Micro In-Line Split Loop Autosampler

Combination of two detectors: PDA-3000 Photodiode Array Detector or VWD-3x00 Variable Wavelength Detector TCC-3100 1x2P-6P Thermostatted Column Compartment with 1x 2-Position 6-Port Switching Valve

The recommended distance between the two towers is approximately 7 cm (2.4 inch).

Important: The modules are primed with 2-propanol. During initial operation of the system, make sure that the solvents used are miscible with 2-propanol. Otherwise, follow the appropriate intermediate steps.


5.3 Flow Schematic The figure below shows the parallel setup. In this setup, two timebases share the column compartment and the autosampler. The autosampler can be switched from one flow path to the other to allow for nearly parallel injections on two columns. Two detectors independently and simultaneously record the separation on both columns

Note: The eluents must be fully miscible, both columns must be operated at the same temperature, the initial eluent conditions on both columns should support fast flow path switching, and the runtimes for both methods should be similar.



5.5 Connecting the Pumps to the TCC-3100 Switching Valve Open the front panels of all modules.

The DGP-3600A or M Dual Low-Pressure Gradient Pump contains two ternary pumps. For further information about how to install the pump, please refer to the Operating Instructions for the pump.

Slightly bend the capillary labeled “TCC port 6 - Pump left” while guiding it through the left opening in the pump bottom. Connect this capillary to the left pump outlet.

Slightly bend the capillary labeled “TCC port 4 - Pump right” while guiding it through the right opening in the pump bottom. Connect this capillary to the right pump outlet.

Outlet Left Pump

Outlet Right Pump



Guide both capillaries between the upper detector and the TCC to the TCC’s internal switching valve.

Connect the capillary from the left pump to port 6 and the capillary from the right pump to port 4 of the TCC’s switching valve.

Right pump to port 4

Left pump to port 6

5.6 Connecting the Analytical or Micro Column and Sampler

Note: Dionex strongly recommends using column ID chip cards (P/N 6710.1505, set of 5 ID chip cards). This allows for easy tracking the column used for each sample.

Install two columns with the flow direction as indicated in the figure above. Below, the upper column is referred to as “column 1”, the bottom column as “column 2”.

After attaching the column IDthe capillary labeled “TCC porto connect column 1 inlet to poTCC valve. Use the capillary laport 3 r. - Col. 2” to connect cto port 3 of the TCC valve.

Column 2 P

Column 1 1

Page 36 Rev. 3.1 © 2007 Dionex

Port

chip card, use t 1 r. - Col. 1” rt 1 of the beled “TCC olumn 2 inlet

ort 3

October 2007

Use the capillary labeled “TCC port 2 – WPS port 4” to connect TCC port 2 to WPS port 4.

Use the capillary labeled “TCC port 5 – WPS port 5” to connect TCC port 5 to WPS port 5.

5.7 Connecting the Detectors The Parallel Operation Capillary Kits, Dual-Ternary Analytical or Micro support both the PDA-3000 Photodiode Detector and the VWD-3x00 Variable Wavelength Detector. The following image refers to the VWD-3x00.

Outlet column 1 to upper detector

Outlet column 2 to bottom detector

Depending on the length of the column, use a capillary labeled “Col. 1 (25 cm) - VWD 1” or a capillary from the detector ship kit to connect the outlet of column 1 to the flow cell inlet of the upper VWD. Proceed accordingly with the capillary labeled “Col. 2 (25 cm) - VWD 2” or a capillary shipped with the detector for connecting column 2 to the bottom VWD. In addition, the application kit contains capillaries labeled “Col. 1 (25 cm) - PDA 1” or “Col. 2 (25 cm) - PDA 2”, respectively, for equivalent use with the PDA-3000. Finally, use any suitable PEEK capillary (not included in the application kit) to connect the outlets of both detectors to the waste. Well done, you have successfully installed the Parallel Operation Capillary Kit, Dual-Ternary Analytical or Micro.


6. 2D-LC (Affinity-Reversed Phase, e.g., IMAC-C18) Kit, Dual-Ternary Analytical (P/N 6037.0008)



This Quick Installation Guide explains the basic installation steps for an analytical UltiMate 3000 Dual-Ternary HPLC System for 2D-LC (Affinity-Reversed Phase, e.g., IMAC-C18). The inner diameter of the capillaries in this application kit is 0.25 mm, except for PEEK collection loop with 0.5 mm ID. For additional information, please refer to the Operating Instructions of the modules.


Description Qty

Capillary Left Pump - TCC port 8 1

Capillary WPS Port 4 - Col. 1 1

Capillary Col. 1 - TCC port 5 1

Capillary Col. 2 (5 cm) - TCC port 9 1




Capillary TCC port 4 - VWD Inlet 1

Capillary TCC port 1 - VWD Outlet 1

Collection loop TCC port 7 - TCC port 10 1


Capillary TCC/PDA analytical 1





Please stack the components as shown in the image on the left: SRD-3600 Solvent Rack and Degasser DGP-3600A Dual-Gradient Analytical Pump WPS-3000SL Analytical In-Line Split Loop Autosampler TCC-3100 1x2P-10P Thermostatted Column Compartment with 1x 2-Position 10-Port Switching Valve PDA-3000 Photodiode Array Detector or VWD-3x00 Variable Wavelength Detector




6.3 Flow Schematic

For further information on an application example using this flow schematic, please refer to Dionex Technical Note TN705 “Automated Enrichment and Determination of Phosphopeptides Using Immobilized Metal Affinity and Reversed-Phase Chromatography with Column Switching“.

6.4 Connecting the SRD-3600 to the DGP-3600A The SRD-3600 Solvent Rack is shipped with six 1-liter eluent bottles, appropriate tubing, and bottle caps. Please refer to the Operating Instructions of the solvent rack for details about how to connect the eluent bottles to the solvent rack and the solvent rack to the pump inlet.


6.5 Connecting the WPS-3000SL

Note: We highly recommend the use of column ID chip cards (P/N 6710.1505, set of 5 ID chip cards). This allows for easy tracking of the columns used for an analysis.

The DGP-3600A/M Dual Low-Pressure Gradient Pump contains two ternary pumps. For further information about how to install the pump, please refer to the Operating Instructions for the pump.

The WPS is shipped with a capillary to connect the pump with the WPS. Guide the capillary through the right opening in the bottom and connect it to the right pump outlet. For further information, please refer to the WPS Operating Instructions.


Outlet Left Pump

Outlet Right Pump


Port 4 Port 5


Install the first dimension column, e.g. a Dionex ProPac IMAC-10, with the flow direction as indicated in the figure above. Below, the upper column is referred to as “column 1”.

After attaching the column ID chip card, use the PEEK capillary labeled “WPS Port 4 – Col. 1” to connect port 4 of the WPS to the inlet of column 1.

Column 1

Inlet column 1

6.6 Connecting the Tubings within the TCC-3100

Use the PEEK capillary labeled “Col. 1 – TCC port 5” to connect the outlet of column 1 to port 5 of the TCC’s switching valve.

Insert the capillary named “Left pump - TCC port 8” by tilting it behind the WPS front panel.

Outlet column 1

Port 5


Guide the capillary through the left opening in the pump bottom.

Mount the capillary at the left side of the system tower using the clamp as indicated by the arrow.

Connect the capillary to port 8 of the 10-port switching valve in the TCC.

The image shows a system including a VWD-3x00. For a VWD system, use the capillary labeled “TCC port 4 – VWD inlet” to connect port 4 to the inlet of the VWD flow cell. For a system including the PDA-3000, use the capillary labeled “6080.2400 Column/Flowcell PDA 0.25x350” accordingly. This capillary is included in the application kit.

Port 4 Port 8

VWD flow cell inlet


Use the capillary labeled “TCC port 1 – VWD outlet” to connect port 1 to the outlet of the VWD flow cell. For a system including the PDA-3000, please use the capillary labeled “6080.2400 Column/Flowcell PDA 0.25x35” from the PDA-3000 ship kit accordingly.

Install the second dimension column, e.g. a Dionex Acclaim 300 C18, with the flow direction as indicated in the figure above. Below, this second column is referred to as “column 2”.

After attaching the column ID chip card, use the capillary labeled “TCC port 3 – Col 2” to connect port 3 of the TCC switching valve to the outlet of column 2.

Install a capillary labeled “TCC port 9 – Col. 2” between port 9 of the TCC switching valve and the inlet of column 2. The application kit contains capillaries for three different column sizes.

Port 1

Column 2 VWD flow cell outlet

Port 3 Port 9

Inlet column 2 Outlet column 2


Use the collection loop labeled “Collection loop - TCC port 7 – TCC port 10” to connect the respective ports of the TCC switching valve.

Connect waste capillaries to both port 2 and port 6 of the TCC switching valve (not included in the application kit).

Port 6

Port 2

Port 10

Port 7

Well done, you have successfully installed the 2D-LC (Affinity-Reversed Phase, e.g., IMAC-C18) Kit, Dual-Ternary Analytical.


7. Tandem Operation Capillary Kits, Dual-Binary Analytical (P/N 6037.0013) or Micro (P/N 6037.0003)



This Quick Installation Guide explains the basic installation steps for an analytical or micro UltiMate 3000 Dual-Binary HPLC System for dual-column tandem mode with off-line column regeneration. The procedure how to install the application kit is identical for both the analytical and the micro version. The only difference between the kits is the inner diameter of the capillaries, i.e., 0.25 mm ID for the analytical version and 0.18 mm ID (from pump outlet) and 0.12 mm ID (all other connections) for the micro version. For additional information, please refer to the Operating Instructions of the modules.

7.1 Included in Delivery Description Qty

Capillary TCC port 8 - Right Pump 1










Capillary TCC port 4 - TCC port 9 1

Capillary TCC 6p / port 2 r. or 10p / port 1 - Det. 1

Tubing Set 2

PEEK Capillary for VWD waste connection 1

Column Clips Kit 1





7.2 Stacking the System Components Please stack the components as shown in the image: SRD-3x00 Solvent Rack and Degasser HPG-3x00A/M Dual-Gradient Analytical/ Micro Pump WPS-3000SL Analytical or Micro In-Line Split Loop Autosampler

HPG-3x00A/M Dual-Gradient Analytical/ Micro Pump TCC-3100 1x2P-10P Thermostatted Column Compartment with 1x 2-Position 10-Port Switching Valve PDA-3000 Photodiode Array Detector or VWD-3x00 Variable Wavelength Detector

The recommended distance between the two towers is approximately 7 cm (2.4 inch). Alternatively, the solvent rack can be placed on the left system tower. However, this Quick Installation Guide discusses only the setup defined above.



7.3 Flow Schematic The figure below shows the principle setup of a dual-column tandem mode configuration. For more detailed information, please refer to the Chromeleon online help.

7.4 Connecting the SRD-3x00 to the HPG-3x00A or M Open the front panels of all modules. The SRD-3x00 Solvent Rack is shipped with eluent bottles, appropriate tubing, and bottle caps. Please refer to the solvent rack manual for details on how to connect the eluent bottles to the solvent rack and the solvent rack to the inlet of the pump below the solvent rack. Possible pump configurations of a Dual-Binary Analytical or Micro system are:

- Two HPG-3200A or M pumps - One HPG-3400A or M pump and one HPG-3200A or M pump - Two HPG-3400A or M pumps

General considerations: The eluent tubings for the pump in the left system tower are provided with the Tandem Operation Capillary Kit for HPG-3200 support. Use the two left outlets of the SRD to connect the SRD to the pump in the left system tower.


The image shows an SRD-3600 for use with an HPG-3200 in the left tower and an HPG-3400 below the solvent rack. This is the preferred setup for a system including the two pump types. For two HPG-3200 pumps, one SRD-3400 is sufficient. For two HPG-3400 pumps, two SRD-3400 solvent racks are required. No additional tubing set is needed.

7.5 Installing the Columns in the TCC-3100

Note: We highly recommend the use of column ID chip cards (P/N 6710.1505, set of 5 ID chip cards). This allows for easy tracking the column used for each sample.

Open the front panels of all modules.

October 2007 Rev. 3.1 Page 51

Port 10 Column 1

Column 2 Port 2

© 2007 Dionex

Install two identical columns with the flow direction as indicated in the figure above. Below, the upper column is referred to as “column 1”, the bottom column as “column 2”. Insert the ID chip cards in any slot.

Use the capillary labeled “TCC port 10 - Col. 1” to connect the outlet of column 1 to port 10 of the TCC valve. Then, use the capillary labeled “TCC port 2 - Col. 2” to connect the outlet of column 2 to port 2 of the TCC valve.

7.6 Further Connections within the TCC-3100

Use the capillary labeled “TCC 6p / port 2 l. or 10p / port 7 - Col. 2 / Col. 1” to connect the inlet of column 1 to port 7. Use the capillary labeled “TCC 6p / port 4 r. or 10p / port 5 - Col. 2” to connect the inlet of column 2 to port 5. Click the rings of the column clamps to finally mount the columns in the oven.

Use the adhesive tape to stick the two capillary clips to the column clamp rings. You may need to slightly cut the adhesive tape in shape. This assembly is for mounting long capillaries within the TCC (see below).

Install the capillary labeled “TCC port 6 – WPS port 4" between port 6 of the TCC and the WPS outlet (port 4).

Use the assembly of column clamp ring and column clip prepared above to mount the capillary in the TCC.

Adhesive tape

Column clamp ring

Capillary clip



Port 6 Port 4


Use the capillary labeled “TCC port 8 - Right pump” to connect outlet of the pump in the right system tower to port 8 of the TCC valve. Guide the capillary through the opening in the pump bottom.

Install the PEEK capillary provided with the application kit in port 3 and connect it to the waste. In addition to the depicted SST nut, an optional PEEK fitting is provided with the kit.

Install the capillary labeled “TCC port 4 - TCC port 9” between port 9 and port 4 of the TCC valve.

Right pump outlet

Port 8 Port 3


7.7 Connecting the Detector

Use the capillary labeled “TCC 6p / port 2 r. or 10p / port 1 - Det.” to connect port 1 with …

…the inlet of the VWD’s flow cell. To connect the PDA-3000, please use the capillary from the detector’s ship kit.

Install a PEEK capillary in the outlet of the flow cell and connect it to the waste (capillary not included in the application kit).

Make sure to guide the capillaries connected to the flow cell through the detector’s capillary slits as depicted above. Proceed accordingly when connecting the PDA-3000.

Port 1 Flow cell inlet

Det. inlet

Capillary slits

Flow cell inlet

Well done, you have successfully installed the Tandem Operation Capillary Kit, Dual-Binary Analytical/Micro.


8. Application Switching Kits, Dual-Binary Analytical (P/N 6037.0011) or Micro (P/N 6037.0012)



This Quick Installation Guide explains the basic installation steps for an analytical or micro UltiMate 3000 Dual-Binary HPLC System for application switching. The procedure how to install the application kit is identical for both the analytical and the micro version. The only difference between the kits is the inner diameter of the capillaries, i.e., 0.25 mm ID for the analytical version and 0.18 mm ID (from pump outlet) and 0.12 mm ID (all other connections) for the micro version. For additional information, please refer to the Operating Instructions of the modules.


Description Qty

Capillary TCC port 4 r. - Pump right 1

Capillary TCC port 6 r. - Pump left 1





Capillary Col. 1 (5 cm) - TCC port 5 l. 1

Capillary Col. 1 (15 cm, 25 cm) - TCC port 5 l. 1

Capillary Col. 2 - TCC port 3 l. 1

Capillary TCC port 2 r.- TCC port 6 l. 1


Capillary TCC port 1 l. - WPS port 4 1

Capillary TCC port 4 l. - Det. 1


Finger-tight 33mm fitting set, 5 pcs 1



8.2 Stacking the System Components Please stack the components as shown in the image: SRD-3x00 Solvent Rack and Degasser HPG-3x00A/M Dual-Gradient Analytical/ Micro Pump WPS-3000SL Analytical or Micro In-Line Split Loop Autosampler

HPG-3x00A/M Dual-Gradient Analytical/ Micro Pump TCC-3100 2x2P-6P Thermostatted Column Compartment with 2x 2-Position 6-Port Switching Valve PDA-3000 Photodiode Array Detector or VWD-3x00 Variable Wavelength Detector

The recommended distance between the two towers is approximately 7 cm (2.4 inch). Alternatively, the modules can be stacked with the solvent rack on the left system tower. This Quick Installation Guide, however, only discusses the setup defined above.



8.3 Flow Schematics The figure below shows the principle of automated application switching. After termination of the first application (“analyze on column # 1”), valve 1 is switched to purge the autosampler with the eluents that will be used for the second application (“wash autosampler”). Valve 2 is then switched, allowing column equilibration and analyses on column 2 to be started (“analyze on column # 2”).

Note: For reasons of clarity, the right TCC valve is shown on the left side of the flow

scheme and the left TCC valve on the right side, respectively. In the figure above, the port positions of valve 2 are shown counter-clockwise. In the real system setup, which is described below, valve 2 is located on the left and valve 1 on the right side of the TCC in order to achieve optimized capillary connections.


8.4 Connecting the SRD-3x00 to the HPG-3x00A or M Open the front panels of all modules. The SRD-3x00 Solvent Rack is shipped with eluent bottles, appropriate tubing, and bottle caps. Please refer to the Operating Instructions of the solvent rack for details about how to connect the eluent bottles to the solvent rack and the solvent rack to the inlet of the pump below the solvent rack. Possible pump configurations of a Dual-Binary Analytical/Micro system are:

- Two HPG-3200A or M pumps - One HPG-3400A or M pump and one HPG-3200A or M pump - Two HPG-3400A or M pumps

General considerations: The eluent tubings for the pump in the left system tower are provided with the Tandem Operation Capillary Kit for HPG-3200 support. Use the two left outlets of the SRD to connect the solvent rack to the pump in the left system tower.

The image shows an SRD-3600 for use with an HPG-3200 in the left tower and an HPG-3400 below the solvent rack. This is the preferred setup for a system including the two pump types. For two HPG-3200 pumps, one SRD-3400 is sufficient. For two HPG-3400 pumps, two SRD-3400 solvent racks are required. No additional tubing set is needed.


8.5 Connecting Both Pumps to the Right TCC Switching Valve

Use the capillary labeled “TCC port 6 r. - Pump left” to connect the outlet of the pump in the left tower to port 6 of the right TCC valve. Guide the capillary through the opening in the bottom of the pump and between the right pump module and the TCC and then into the column compartment.

To connect the pump in the right tower, use the capillary labeled “TCC port 4 r. – Pump right” to connect the outlet of the right pump to port 4 of the right TCC valve. Guide the capillary through the opening in the bottom of the pump.

Outlet left pump

Port 6

Outlet right pump

Port 4

8.6 Installing Capillaries within the TCC

Note: We highly recommend the use of column ID chip cards (P/N 6710.1505, set of 5 ID chip cards). This allows for easy tracking the column used for each sample.


Use the capillary provided with the VWD to connect port 4 of the left switching valve to the inlet of the flow cell. For connecting the PDA-3000, use the capillary labeled “TCC port 4 l. - PDA” to connect port 4 of the left valve to the inlet of the PDA flow cell.

Place column 1 on the open TCC cover with the orientation of the flow direction as indicated in the image. After attaching and inserting the column ID chip card, connect a capillary labeled “Col. 1 - TCC port 5 l." on the left and a capillary labeled “TCC port 1 r. - Col. 1” on the right and screw them into the respective ports. The application kit includes optimized capillary lengths for connecting different column lengths.

Click the rings of the column clamps to finally mount column 1. Then, insert column 2 with the indicated flow orientation and insert its ID chip card in a slot. Use the capillary labeled “Col. 2 - TCC port 3 l.” to connect the outlet of column 2 to port 3 of the left valve.

Use the capillary labeled “TCC 6p / port 2 l. or 10p / port 7 - Col. 2 / Col. 1” to connect the column’s inlet to port 2 of the left switching valve. Click the rings of the column clamps to finally mount column 2.

Port 5 Port 1

Port 4

VWD flow cell inlet

Column 2 outlet Port 2

Column 2 inlet

Port 3


Use the capillary labeled “TCC port 2 r.- TCC port 6 l.” to connect the left valve (port 6) and the right valve (port 2).

Use port 3 of the right valve to connect the waste capillary provided with the application kit.

Port 6 Port 2

Port 3

8.7 Connecting the Autosampler

Use the capillary labeled “TCC port 1 l. - WPS port 4” to connect port 4 of the WPS to port 1 of the left TCC valve.

Use the adhesive tape to stick the two capillary clips to the column clamp rings. You may need to slightly cut the adhesive tape in shape. This assembly is for mounting long capillaries within the TCC (see below).

Adhesive tape

Column clamp ring

Capillary clip

Port 4

Port 1


Use the capillary labeled “TCC port 5 – WPS port 5” to connect the WPS valve (port 5) and the right TCC valve (port 5).

Use one or two assemblies of column clamp ring and column clip prepared above to mount the capillary in the TCC.

Port 5 Port 5

8.8 Installing the Detector’s Waste Capillary

Finally, install a PEEK capillary in the outlet of the flow cell and connect it to the waste (capillary not included in the application kit). Make sure to guide the capillaries connected to the flow cell through the detector’s capillary slits as depicted above. Proceed accordingly when connecting the PDA-3000.

Capillary slits

Well done, you have successfully installed the Application Switching Kits, Dual-Binary Analytical or Micro.


9. Automated Method Scouting, Quaternary Analytical (P/N 6722.0101) or Micro (P/N 6722.0100)



This Quick Installation Guide explains the basic installation steps for an analytical or micro UltiMate© 3000 system for automated method development (column scouting). The procedure how to install the application kit is identical for both the analytical and micro version. The only difference between the application kits is the inner diameter of the capillaries, i.e., 0.25 mm ID for the analytical version and 0.18 mm ID (from pump outlet) and 0.12 mm ID (all other connections) for the micro version. For additional information, please refer to the operating instructions of the modules.

9.1 Included in Delivery The kit for Automated Method Scouting contains all the required tubing to get started. For analytical columns with a length of 5 or 15 cm, stainless steel tubing is supplied in the kit. For 25 cm columns PEEK tubing is supplied, for columns with a different length a combination of stainless steel (preferred for inlet) and PEEK tubing can be used. More detailed information is listed in the table on page 72. Stainless steel tubing for an optional eluent preheater in the TCC is also included in the kit. The preheater must be installed on position four (top right position), for details refer to the eluent preheater installation guide. The column named ‘position’ in the table on the next page refers to the position of the capillaries in the flow schematic (Page 69, Chapter 9.3)


Position Description Label Quantity

Pump outlet, A Pump capillary to WPS

WPS port 5 1

WPS port 4 B WPS capillary to center ValveRight

TCC Center r. 1

WPS port 4 B' WPS capillary to Eluent Preheater Inlet

Preheater in 1

Preheater out B' Eluent Preheater Outlet to center ValveRight

TCC center r. 1

TCC port 1 r. C ValveRight Port 1 to Column 1 Inlet for 5 cm columns

5 cm Col. 1 in 1

TCC port 1 r. C ValveRight Port 1 to Column 1 Inlet for 15 cm columns

15 cm Col. 1 in 1

TCC port 2 r. D ValveRight Port 2 to Column 2 Inlet for 5 cm columns

5 cm Col. 2 in 1

TCC port 2 r. D ValveRight Port 2 to Column 2 Inlet for 15 cm columns

15 cm Col. 2 in 1

TCC port 3 r. E ValveRight Port 3 to Column 3 Inlet for 5 cm columns

5 cm Col. 3 in 1

TCC port 3 r. E ValveRight Port 3 to Column 3 Inlet for 15 cm columns

15 cm Col. 3 in 1

TCC port 4 r. F ValveRight Port 4 to Column 4 Inlet for 5 cm columns

5 cm Col. 4 in 1

TCC port 4 r. F ValveRight Port 4 to Column 4 Inlet for 15 cm columns

15 cm Col. 4 in 1

TCC port 5 r. G ValveRight Port 5 to Column 5 Inlet for 5 cm columns

5 cm Col. 5 in 1

TCC port 5 r. G ValveRight Port 5 to Column 5 Inlet for 15 cm columns

15 cm Col. 5 in 1

TCC port 6 r. H ValveRight Port 6 to Column 6 Inlet for 5 cm columns

5 cm Col. 6 in 1

TCC port 6 r. H ValveRight Port 6 to Column 6 Inlet for 15 cm columns

15 cm Col. 6 in 1

B’ tubing to replace the direct connection of WPS-3000 to the TCC in case an eluent preheater is installed Table continues on next page.


Table of capillaries included in delivery (continued).

Position Description Label Quantity

Col. 1 out I Column 1 Outlet to ValveLeft Port 1 for 5 and 15 cm columns TCC port 1 l.

1

Col. 2 out J Column 2 Outlet to ValveLeft Port 2 for 5 and 15 cm columns

TCC port 2 l. 1

Col. 3 out K Column 3 Outlet to ValveLeft Port 3 for 5 and 15 cm columns

TCC port 3 l. 1

Col. 4 out L Column 4 Outlet to ValveLeft Port 4 for 5 and 15 cm columns

TCC port 4 l. 1

Col. 5 out M Column 5 Outlet to ValveLeft Port 5 for 5 and 15 cm columns

TCC port 5 l. 1

Col. 6 out N Column 6 Outlet to ValveLeft Port 6 for 5 and 15 cm columns

TCC port 6 l. 1

TCC center l. O ValveLeft Center Port to VWD-3x00 Detector

Det. VWD 1

TCC center l. O ValveLeft Center Port to PDA-3000 Detector

Det. PDA 1

Incl.with. P Waste line from Detector Detector

TCC valve r. PEEK 25 cm Col.

C, D, E, F, G, H ValveRight to Column Inlet for 25 cm columns, PEEK 12 cm

Col. in 6

Col. out PEEK 25 cm Col.

I, J, K, L, M, N Column Outlet to ValveLeft for 25 cm columns PEEK 12 cm

TCC valve l. 6

Col. out PEEK tubing

I, J, K, L, M, N Column Outlet to ValveLeft other sized columns PEEK 34 cm

TCC valve l. 6

TCC valve l. Bypass tubing Multi Position Valve

TCC valve r. 1



Please stack the components as shown in the image on the left: SR-3000 Solvent Rack LPG-3400A Quaternary Analytical Pump with Degasser or LPG-3400M Quaternary Micro Pump with Degasser WPS-3000SL Analytical or Micro In-Line Split Loop Autosampler TCC-3200 2x6P-7P Thermostatted Column Compartment with 2x 6-Position 7-Port Multi-Position Switching Valve PDA-3000 Photodiode Array Detector or VWD-3x00 Variable Wavelength Detector



9.3 Flow Schematic The next figure below shows the schematic of the LC set-up for automated method scouting. The heart of the system is the TCC 3200 with two 6-position 7-port valves.

Schematic for Automated Method Scouting. .

The schematic depicts six columns without eluent preheater installed in the column compartment. Depending on the valve position one analytical column at a time is active for analyses. In the schematic the active flow path for Column 1 is depicted. For further information about the Automated Method Scouting application, please refer to the LCi solutions manual.

Note: It is very much recommended to make the various stainless steel connections in the order as described in this Quick Installation Guide for Automated Method Scouting.

Note: The analytical columns are numbered from top to bottom, one to six. Column one is connected to port 1 of the right and left valve, column two is connected to port 2 of the right and left valve, and so on. Connecting one column to different port number for the left and right valve could introduce problems when using the automation features of Chromeleon.


9.4 Connecting the LPG-3400A or M Pump to the WPS-3000SL Sampler

Install the pump and degasser according to the Operating Instructions of the LPG-3400 and SR-3000. Place the Solvent Rack on top of the pump and guide the pump tubing to the Solvent Rack The outlet of the pump is connected to the WPS port 5 of the injection valve with the tubing labeled Pump outlet - WPS port 5. .

The outlet of the Pump is located at the pressure sensor. Guide the tubing labeled Pump outlet - WPS port 5 through the right opening of the pump base to the WPS valve

Connect the tubing labeled Pump outlet - WPS port 5 from the pump outlet to port 5 of the WPS valve.


9.5 Connecting the WPS-3000SL to the Column Compartment

WITHOUT PREHEATER: The tubing labeled WPS port 4 - TCC center r. from the WPS is connected to the right valve center port of the TCC.

Tip: Connect this tubing after connecting the tubing for the columns.

WITHOUT PREHEATER: Connect the tubing labeled WPS port 4 - TCC center r. on port 4 of the WPS. Use the supplied clamp to guide the tubing to the TCC. WITH PREHEATER: Connect the tubing labeled WPS port 4 - Preheater in on port 4 of the WPS. Use the supplied clamp to guide the tubing to the TCC.

WITH ELUENT PREHEATER: The tubing labeled WPS port 4 - Preheater in from the autosampler is connected to the eluent preheater inlet.

Tip: Connect this tubing before connecting the tubing for the columns.


9.6 Connecting the Analytical Columns in the Column Compartment

It is recommended to assemble first the nut and ferrule on the tubing on the in- and outlet of the analytical column before connecting the fluidic lines in the column compartment. For information about which nut and ferrule to use, see page 3 and 4. The connection of the tubing starts with connecting the outlet of the analytical columns to the left valve. Stainless steel tubing is used to connect the columns with a length of 5 cm or 15 cm to the valves in the column compartment. Precut PEEK tubing is supplied to connect 25 cm columns. Columns with a different length can be connected with PEEK tubing or a combination of stainless steel (preferred for the inlet) and PEEK tubing. The supplied PEEK tubing labeled Col. out - PEEK tubing - TCC valve l. has to be shortened.to the appropriate length for the selected column using a PEEK cutter. Make sure the tubing is long enough to install it with fittings without creating sharp bends in the PEEK tubing. The tubing labeled TCC valve l. – TCC valve r. can be used as bypass, in place of an analytical column. This bypass tubing can be used to quickly flush the system if e.g., incompatible mobile phases are used. Recommended connection tubing for various column lengths:

Column Length Outlet tubing label Inlet Tubing label

5cm and 15cm and

Connection of the column outlet to the left valve for 5 cm or 15 cm columns with stainless steel connections. For convenient handling it is recommended to follow the order described below.

1. Tubing labeled Col. 4 out - TCC port 4 l. is used to connect column four outlet to the left valve port 4 of the TCC.

2. Tubing labeled Col. 5 out - TCC port 5 l. is used to connect column five outlet to the left valve port 5 of the TCC.

3. Tubing labeled Col. 6 out - TCC port 6 l. is used to connect column six outlet to the left valve port 6 of the TCC.

4. Tubing labeled Col. 1 out - TCC port 1 l. is used to connect column one outlet to the left valve port 1 of the TCC.

5. Tubing labeled Col. 2 out - TCC port 2 l. is used to connect column two outlet to the left valve port 2 of the TCC.

6. Tubing labeled Col. 3 out - TCC port 3 l. is used to connect column three outlet to the left valve port 3 of the TCC.


Connections of the right valve to the column inlet, start with the white marked tubing and follow the order described below for convenient handling.


WITH ELUENT PREHEATER: 1. Connect the tubing labeled TCC port 2 r.

– 5 cm Col. 2 in for 5 cm columns or TCC port 2 r. – 15 cm Col. 2 in for 15 cm columns from the right valve to the column.

2. Connect the tubing labeled TCC port 3 r. – 5 cm Col. 3 in for 5 cm columns or TCC port 3 r. – 15 cm Col. 3 in for 15 cm columns from the right valve to the column.




6. Connect the tubing labeled Preheater out – TCC Center r. from the preheater outlet to the right valve.


WITHOUT ELUENT PREHEATER: 1. Connect the tubing labeled TCC port 1 r.

– 5 cm Col. 1 in for 5 cm columns or TCC port 1 r. – 15 cm Col. 1 in for 15 cm columns from the right valve to the column.






7. The tubing labeled WPS port 4 - TCC center r. from the WPS is connected to the right valve center port of the TCC.

9.7 Connecting the Column Compartment to the Detector The PDA-3000 detector is connected from the center port of the left valve with tubing labeled TCC center l. - Det. PDA. The outlet of the PDA flow cell is connected with tubing from the PDA-3000 accessory kit.

The inlet of the PDA flow cell is connected via the tubing labeled TCC center l. - Det. PDA from the left valve center port. The outlet of the flow cell is connected with tubing supplied with the PDA-3000 accessory kit.


The VWD-3x00 detector is connected from the center port of the left valve with tubing labeled TCC center l. - Det. VWD. The outlet of the VWD flow cell is connected with tubing supplied with the accessory kit of the VWD-3x00.

The inlet of the VWD flow cell is connected via the tubing labeled TCC center l. - Det. VWD from the left valve center port. The outlet of the flow cell is connected with tubing supplied with the accessory kit of the VWD-3x00.


Well done, you have successfully installed the UltiMate© 3000 system for automated method development. After installation the column compartment could look like this. Depicted is the installation of six 15cm columns in the column compartment with inclusive an eluent preheater.

Or after installation the column compartment could look like this. Maximum flexibility, from top to bottom:

1. 25cm column with PEEK tubing inlet and outlet 2. 5cm column with stainless steel tubing inlet and outlet 3. 3cm column with stainless steel tubing inlet, PEEK tubing outlet 4. 5cm column with stainless steel tubing inlet and outlet 5. 7.5cm column with stainless steel tubing inlet, PEEK tubing outlet 6. 10cm column with stainless steel tubing inlet, PEEK tubing outlet


IntroductionGeneral System PreparationConnecting the Eluent Line to the Solvent ReservoirWhen to use Which ConnectorUsing 10-Port Instead of 6-Port TCC-3x00 Valves

Tandem Operation Capillary Kits, Dual-Ternary Analytical (P/Included in DeliveryStacking the System ComponentsFlow SchematicConnecting the SRD-3600 to the DGP-3600A or MConnecting the Left Pump to the TCC-3100Connecting the Right Pump to the WPS-3000SLInstalling Capillaries within the TCC-3100Connecting the Detector

Application Switching Kits, Dual-Ternary Analytical (P/N 603Included in DeliveryStacking the System ComponentsFlow SchematicsConnecting the SRD-3600 to the DGP-3600AConnecting the Columns within the TCC-3200Connecting the Pumps

On-line Sample Preparation Capillary Kits, Dual-Ternary AnalIncluded in DeliveryStacking the System ComponentsFlow SchematicsConnecting the SRD-3600 to the DGP-3600A or MConnecting the WPS-3000SLConnecting the Analytical or Micro ColumnSetup with PDA-3000Setup with VWD-3100/VWD-3400

Installing the SPE Column

Parallel Operation Capillary Kits, Dual-Ternary Analytical (Included in DeliveryStacking the System ComponentsFlow SchematicConnecting the SRD-3600 to the DGP-3600A or MConnecting the Pumps to the TCC-3100 Switching ValveConnecting the Analytical or Micro Column and SamplerConnecting the Detectors

2D-LC (Affinity-Reversed Phase, e.g., IMAC-C18) Kit, Dual-TeIncluded in DeliveryStacking the System ComponentsFlow SchematicConnecting the SRD-3600 to the DGP-3600AConnecting the WPS-3000SLConnecting the Tubings within the TCC-3100

Tandem Operation Capillary Kits, Dual-Binary Analytical (P/NIncluded in DeliveryStacking the System ComponentsFlow SchematicConnecting the SRD-3x00 to the HPG-3x00A or MInstalling the Columns in the TCC-3100Further Connections within the TCC-3100Connecting the Detector

Application Switching Kits, Dual-Binary Analytical (P/N 6037Included in DeliveryStacking the System ComponentsFlow SchematicsConnecting the SRD-3x00 to the HPG-3x00A or MConnecting Both Pumps to the Right TCC Switching ValveInstalling Capillaries within the TCCConnecting the AutosamplerInstalling the Detector’s Waste Capillary

Automated Method Scouting, Quaternary Analytical (P/N 6722.0Included in DeliveryStacking the System ComponentsFlow SchematicConnecting the LPG-3400A or M Pump to the WPS-3000SL SamplerConnecting the WPS-3000SL to the Column CompartmentConnecting the Analytical Columns in the Column CompartmentConnecting the Column Compartment to the Detector

ultimate 3000 x2 system capillary...

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