Two New Transfection Reagents Go Xtreme!

For life science research only. Not for use in diagnostic procedures.

Figure 2: X-tremeGENE HP DNA Transfection Reagent outperforms

reagent LTX P. Four hard-to-transfect cell lines were transfected in serum-containing medium using X-tremeGENE HP Reagent. Transfection efficiency was measured as a ratio of GFP-transfected cells versus whole cell number using the Cellavista System and normalized to the reference reagent set at 1. Error bars show the standard deviation of the mean of triplicates.

For more information about X-tremeGENE HP Reagent, product selection table and cell-type specific transfection protocols, please visit our Transfection Special Interest Site at X-tremeGENE.roche.com

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X-tremeGENE HPLTX P

15

10

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0PC-3 HT-1080 RAW CRL K-562

HEK-293 K-562 HeLa

Figure 1: High level of GFP expression using X-tremeGENE HP DNA

Transfection Reagent in various cell lines. HEK-293, K-562, and HeLa cells were transfected in serum-containing medium using X-tremeGENE HP DNA Transfection Reagent, and analyzed using the Roche Cellavista System. All cell types showed highest levels of GFP expression when using X-tremeGENE HP DNA Transfection Reagent.

New X-tremeGENE HP Reagent – High Performance DNA Transfection

Improve your success rate with X-tremeGENE HP DNA Transfection Reagent from Roche.This new high-performance reagent is an optimized formulation for transfecting a broad range of eukaryotic cells, including insect cells and many hard-to-transfect cell lines, including MEF, MCF7, HepG2, HeLa, K-562, and U-937.

Achieve new levels of transfection efficiency for difficult-to-transfect cell lines (see Figures 1 and 2).

Benefit from an easy-to-use reagent, free of animal-derived components and active in serum-containing medium.

Increase experimental throughput using a simple and fast protocol (see Figure 3).

Requ

est your sample at:

X-tremeGENE.roche.co

m

Free Sample

For life science research only. Not for use in diagnostic procedures.

X-TREMEGENE and CELLAVISTA are trademarks of Roche.Other brands or product names are trademarks of their respective holders.

Published byRoche Diagnostics GmbHSandhofer Straße 11668305 MannheimGermany

© 2010 Roche Diagnostics. All rights reserved.

www.roche-applied-science.com

06482317001 � 1010

Product Cat. No. Pack Size

X-tremeGENE HP DNA Transfection Reagent

06 366 244 00106 366 236 00106 366 546 001

0.4 ml1.0 ml5 x 1 ml

X-tremeGENE 9 DNA Transfection Reagent

06 365 779 00106 365 787 00106 365 809 001

0.4 ml1.0 ml5 x 1 ml

New X-tremeGENE 9 Reagent – High Cell Survival

Due to its low cytotoxicity, minimal optimization, and high transfection efficiency in commonly used cell lines, e.g. CHO-K1, HEK-293, NIH-3T3 and COS-7, even in the presence of serum, X-tremeGENE 9 DNA Transfection Reagent is perfectly suited for applications in all fields of cellular analysis.

For more information about X-tremeGENE 9 Reagent, product selection table and cell-type specific transfection protocols, please visit our Transfection Special Interest Site at X-tremeGENE.roche.com

Figure 4: High cell survival using X-tremeGENE 9 DNA Transfection Reagent. HeLa cells were transfected with X-tremeGENE 9 Reagent and two different competitor reagents. Reagent cytotoxicity was measured using the Roche LDH Cytotoxicity Detection Kit (LDH). The applied ratios of µl reagent to µg DNA are indicated. X-tremeGENE 9 DNA Transfection Reagent showed the lowest cytotoxic effect.

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2:1

X-tremeGENE 9 DNATransfection Reagent

Competitor L2K Competitor LTX P

3:1 2:1 1,5:1:1 3:1:13:1

� Obtain maximum cell viability after transfection using X-tremeGENE 9 Reagent (see Figure 4).

� Generate physiologically relevant data you can trust using a reagent with low cytotoxic effects (see Figure 4).

� Save time and effort, and avoid time-consuming optimization procedures (see Figure 3).

Figure 3: Transfection procedure. Simply mix X-tremeGENE HP or 9DNA Transfection Reagent with plasmid DNA, and pipet onto your cells even in the presence of serum.

Fewer Steps = Lower Costs!

Add DNA/X-tremeGENETransfection Reagentmix to each well

Remove serum-containing medium from each well

Add serum-free medium to each well

Add DNA/Transfection Reagent mix to each well

Remove DNA/Transfection Reagent mix from each well

Replace serum-containing medium in each well

X-tremeGENE Transfection Reagents HP and 9

Classical Liposomal Transfection Reagents