pH time curve of various buffers

Proceedure : In a beaker take 210 ml of water .to this add 9.6 ml of 0.1 N HCL and aalow to stirr with magnetic stirrer(basal stomach fluid)measure the initial pH (about 2.4),add the required qty. of buffer as determined by pH neutralizing capacity of different buffers.Titrate the buffer solution with 0.1 N HCL (30ml) for a period of 60 minutes at the rate of 0.5 ml per minute. Note the change in pH at every 2 min interval for soluble buffersand 5 mint. Interval for insoluble bufferes.

Observaton: Initially 135 mg was taken the pH was found to be 7.01another 165 mg was added to the same vessel the pH was found to be 7.91another 200 mg was added to the same vessel the pH was found to be 8.24finally another 500 mg was added to the same vessel the pH was found to be 8.62

TrisWt. taken = 1 1002.6 mg Initial = 2.4 pH

Time Tris 1002.5 mg0 8.62

10 8.4920 8.3930 8.2940 8.250 8.1260 8.04

0 10 20 30 40 50 60 700

1

2

3

4

5

6

7

8

9

10

Tris 1002.5 mg

Tris 1002.5 mg

Time in minutes

pH

In a beaker take 210 ml of water .to this add 9.6 ml of 0.1 N HCL and aalow to stirr with magnetic stirrer(basal stomach fluid)measure the initial pH (about 2.4),add the required qty. of buffer as determined by pH neutralizing capacity of different buffers.Titrate the buffer solution with 0.1 N HCL (30ml) for a period of 60 minutes at the rate of 0.5 ml per minute. Note the change in pH at every 2 min interval for soluble buffers

another 165 mg was added to the same vessel the pH was found to be 7.91another 200 mg was added to the same vessel the pH was found to be 8.24finally another 500 mg was added to the same vessel the pH was found to be 8.62

0 10 20 30 40 50 60 700

1

2

3

4

5

6

7

8

9

10

Tris 1002.5 mg

Tris 1002.5 mg

Time in minutes

pH

pH time curve of various buffers

ProceedureIn a beaker take 210 ml of water .to this add 9.6 ml of 0.1 N HCL and aalow to stirr with magnetic stirrer(basal stomach fluid)measure the initial pH (about 2.4),add the required qty. of buffer as determined by pH neutralizing capacity of different buffers.Titrate the buffer solution with 0.1 N HCL (30ml) for a period of 60 minutes at the rate of 0.5 ml per minute. Note the change in pH at every 2 min interval for soluble buffersand 5 mint. Interval for insoluble bufferes.

Trisodium phosphate Wt. taken = 160.5 mg Initial = 2.4 pH

Time TSP 160.5 mg Tris 1000mg0 7.83

10 6.4620 3.0230 2.240 1.9850 1.8460 1.74

0 10 20 30 40 50 60 700

1

2

3

4

5

6

7

8

9

pH time curve of TSP

TSP 160.5 mg

Time in minutes

pH

In a beaker take 210 ml of water .to this add 9.6 ml of 0.1 N HCL and aalow to stirr with magnetic stirrer(basal stomach fluid)measure the initial pH (about 2.4),add the required qty. of buffer as determined by pH neutralizing capacity of different buffers.Titrate the buffer solution with 0.1 N HCL (30ml) for a period of 60 minutes at the rate of 0.5 ml per minute. Note the change in pH at every 2 min interval for soluble buffers

0 10 20 30 40 50 60 700

1

2

3

4

5

6

7

8

9

pH time curve of TSP

TSP 160.5 mg

Time in minutes

pH

pH time curve of various buffers

Proceedure : In a beaker take 210 ml of water .to this add 9.6 ml of 0.1 N HCL and aalow to stirr with magnetic stirrer(basal stomach fluid)measure the initial pH (about 2.4),add the required qty. of buffer as determined by pH neutralizing capacity of different buffers.Titrate the buffer solution with 0.1 N HCL (30ml) for a period of 60 minutes at the rate of 0.5 ml per minute. Note the change in pH at every 2 min interval for soluble buffersand 5 mint. Interval for insoluble bufferes.

Observaton: Initially 135 mg was taken the pH was found to be 7.01another 165 mg was added to the same vessel the pH was found to be 7.91another 200 mg was added to the same vessel the pH was found to be 8.24finally another 500 mg was added to the same vessel the pH was found to be 8.62

Calcium carbonateWt. taken = 1 280.40 mg Initial = 2.4 pH

Time CaCo3 280.4mg0 8.17

10 7.8420 7.7430 7.6340 7.650 7.5560 7.3

0 10 20 30 40 50 60 700

1

2

3

4

5

6

7

8

9

CaCo3 280.4mg

CaCo3 280.4mg

Time in minutes

pH

In a beaker take 210 ml of water .to this add 9.6 ml of 0.1 N HCL and aalow to stirr with magnetic stirrer(basal stomach fluid)measure the initial pH (about 2.4),add the required qty. of buffer as determined by pH neutralizing capacity of different buffers.Titrate the buffer solution with 0.1 N HCL (30ml) for a period of 60 minutes at the rate of 0.5 ml per minute. Note the change in pH at every 2 min interval for soluble buffers

another 165 mg was added to the same vessel the pH was found to be 7.91another 200 mg was added to the same vessel the pH was found to be 8.24finally another 500 mg was added to the same vessel the pH was found to be 8.62

0 10 20 30 40 50 60 700

1

2

3

4

5

6

7

8

9

CaCo3 280.4mg

CaCo3 280.4mg

Time in minutes

pH

pH time curve of various buffers

Proceedure : In a beaker take 210 ml of water .to this add 9.6 ml of 0.1 N HCL and aalow to stirr with magnetic stirrer(basal stomach fluid)measure the initial pH (about 2.4),add the required qty. of buffer as determined by pH neutralizing capacity of different buffers.Titrate the buffer solution with 0.1 N HCL (30ml) for a period of 60 minutes at the rate of 0.5 ml per minute. Note the change in pH at every 2 min interval for soluble buffersand 5 mint. Interval for insoluble bufferes.

Observaton:

Magnesium HydroxideWt. taken = 150.7 mg Initial = 2.4 pH

Time MgOH2 150.7mg0 10.03

10 9.8420 9.6930 9.6140 9.4550 9.2560 9

0 10 20 30 40 50 60 700

2

4

6

8

10

12

MgOH2 150.7mg

MgOH2 150.7mg

Time in minutes

pH

In a beaker take 210 ml of water .to this add 9.6 ml of 0.1 N HCL and aalow to stirr with magnetic stirrer(basal stomach fluid)measure the initial pH (about 2.4),add the required qty. of buffer as determined by pH neutralizing capacity of different buffers.Titrate the buffer solution with 0.1 N HCL (30ml) for a period of 60 minutes at the rate of 0.5 ml per minute. Note the change in pH at every 2 min interval for soluble buffers

0 10 20 30 40 50 60 700

2

4

6

8

10

12

MgOH2 150.7mg

MgOH2 150.7mg

Time in minutes

pH

pH time curve of various buffers

Proceedure : In a beaker take 210 ml of water .to this add 9.6 ml of 0.1 N HCL and aalow to stirr with magnetic stirrer(basal stomach fluid)measure the initial pH (about 2.4),add the required qty. of buffer as determined by pH neutralizing capacity of different buffers.Titrate the buffer solution with 0.1 N HCL (30ml) for a period of 60 minutes at the rate of 0.5 ml per minute. Note the change in pH at every 2 min interval for soluble buffersand 5 mint. Interval for insoluble bufferes.

Observaton: Double the qty. of neutralizing capacity

Sodium bi carbonateWt. taken = 350 mg Initial = 2.4 pH

Time NaHCO 3 350mg0 8.54

10 8.2820 8.0830 8.0140 7.8450 7.5560 6.68

0 10 20 30 40 50 60 700

1

2

3

4

5

6

7

8

9

NaHCO 3 350mg

NaHCO 3 350mg

Time in minutes

pH

In a beaker take 210 ml of water .to this add 9.6 ml of 0.1 N HCL and aalow to stirr with magnetic stirrer(basal stomach fluid)measure the initial pH (about 2.4),add the required qty. of buffer as determined by pH neutralizing capacity of different buffers.Titrate the buffer solution with 0.1 N HCL (30ml) for a period of 60 minutes at the rate of 0.5 ml per minute. Note the change in pH at every 2 min interval for soluble buffers

0 10 20 30 40 50 60 700

1

2

3

4

5

6

7

8

9

NaHCO 3 350mg

NaHCO 3 350mg

Time in minutes

pH

pH time curve of various buffers

Proceedure : In a beaker take 210 ml of water .to this add 9.6 ml of 0.1 N HCL and aalow to stirr with magnetic stirrer(basal stomach fluid)measure the initial pH (about 2.4),add the required qty. of buffer as determined by pH neutralizing capacity of different buffers.Titrate the buffer solution with 0.1 N HCL (30ml) for a period of 60 minutes at the rate of 0.5 ml per minute. Note the change in pH at every 2 min interval for soluble buffersand 5 mint. Interval for insoluble bufferes.

Observaton:

Magnesium Oxide heavyWt. taken = 170.9 mg Initial = 2.4 pH

Time MgO heavy 170.9 mg0 10.66

10 10.6120 10.5930 10.5740 10.5650 10.5460 10.5

0 10 20 30 40 50 60 700

2

4

6

8

10

12

MgO heavy 170.9 mg

MgO heavy 170.9 mg

Time in minutes

pH

In a beaker take 210 ml of water .to this add 9.6 ml of 0.1 N HCL and aalow to stirr with magnetic stirrer(basal stomach fluid)measure the initial pH (about 2.4),add the required qty. of buffer as determined by pH neutralizing capacity of different buffers.Titrate the buffer solution with 0.1 N HCL (30ml) for a period of 60 minutes at the rate of 0.5 ml per minute. Note the change in pH at every 2 min interval for soluble buffers

0 10 20 30 40 50 60 700

2

4

6

8

10

12

MgO heavy 170.9 mg

MgO heavy 170.9 mg

Time in minutes

pH

pH time curve of various buffers

Proceedure : In a beaker take 210 ml of water .to this add 9.6 ml of 0.1 N HCL and aalow to stirr with magnetic stirrer(basal stomach fluid)measure the initial pH (about 2.4),add the required qty. of buffer as determined by pH neutralizing capacity of different buffers.Titrate the buffer solution with 0.1 N HCL (30ml) for a period of 60 minutes at the rate of 0.5 ml per minute. Note the change in pH at every 2 min interval for soluble buffersand 5 mint. Interval for insoluble bufferes.

Observaton:

TSP + MgOWt. taken = 160 TSP + 170.1 MgO Initial = 2.4 pH

Time 160 TSP + 170.1 MgO0 10.66

10 10.6520 10.6930 10.6740 10.5750 10.5460 10.38

0 10 20 30 40 50 60 700

2

4

6

8

10

12

160 TSP + 170.1 MgO

160 TSP + 170.1 MgO

Time in minutes

pH

In a beaker take 210 ml of water .to this add 9.6 ml of 0.1 N HCL and aalow to stirr with magnetic stirrer(basal stomach fluid)measure the initial pH (about 2.4),add the required qty. of buffer as determined by pH neutralizing capacity of different buffers.Titrate the buffer solution with 0.1 N HCL (30ml) for a period of 60 minutes at the rate of 0.5 ml per minute. Note the change in pH at every 2 min interval for soluble buffers

0 10 20 30 40 50 60 700

2

4

6

8

10

12

160 TSP + 170.1 MgO

160 TSP + 170.1 MgO

Time in minutes

pH

pH time curve of various buffers

Proceedure : In a beaker take 210 ml of water .to this add 9.6 ml of 0.1 N HCL and aalow to stirr with magnetic stirrer(basal stomach fluid)measure the initial pH (about 2.4),add the required qty. of buffer as determined by pH neutralizing capacity of different buffers.Titrate the buffer solution with 0.1 N HCL (30ml) for a period of 60 minutes at the rate of 0.5 ml per minute. Note the change in pH at every 2 min interval for soluble buffersand 5 mint. Interval for insoluble bufferes.

Observaton:

TSP + CaCO3Wt. taken = 160 TSP + CaCO3 280.4 mg Initial = 2.4 pH

Time 160 TSP + CaCO3 280.4 mg0 9.48

10 7.420 6.9830 6.7540 6.5950 6.4760 6.44

0 10 20 30 40 50 60 700

1

2

3

4

5

6

7

8

9

10

160 TSP + CaCO3 280.4 mg

160 TSP + CaCO3 280.4 mg

Time in minutes

pH

In a beaker take 210 ml of water .to this add 9.6 ml of 0.1 N HCL and aalow to stirr with magnetic stirrer(basal stomach fluid)measure the initial pH (about 2.4),add the required qty. of buffer as determined by pH neutralizing capacity of different buffers.Titrate the buffer solution with 0.1 N HCL (30ml) for a period of 60 minutes at the rate of 0.5 ml per minute. Note the change in pH at every 2 min interval for soluble buffers

0 10 20 30 40 50 60 700

1

2

3

4

5

6

7

8

9

10

160 TSP + CaCO3 280.4 mg

160 TSP + CaCO3 280.4 mg

Time in minutes

pH

pH time curve of various buffers

Proceedure : In a beaker take 210 ml of water .to this add 9.6 ml of 0.1 N HCL and aalow to stirr with magnetic stirrer(basal stomach fluid)measure the initial pH (about 2.4),add the required qty. of buffer as determined by pH neutralizing capacity of different buffers.Titrate the buffer solution with 0.1 N HCL (30ml) for a period of 60 minutes at the rate of 0.5 ml per minute. Note the change in pH at every 2 min interval for soluble buffersand 5 mint. Interval for insoluble bufferes.

Observaton:

TSP + MgOH2Wt. taken = 160.6 TSP + MgOH2 150.3 mg Initial = 2.4 pH

Time 160.6 TSP + MgOH2 150.3 mg0 9.88

10 9.320 8.8630 8.5640 8.6450 8.5360 8.43

0 10 20 30 40 50 60 700

2

4

6

8

10

12

160.6 TSP + MgOH2 150.3 mg

160.6 TSP + MgOH2 150.3 mg

Time in minutes

pH

In a beaker take 210 ml of water .to this add 9.6 ml of 0.1 N HCL and aalow to stirr with magnetic stirrer(basal stomach fluid)measure the initial pH (about 2.4),add the required qty. of buffer as determined by pH neutralizing capacity of different buffers.Titrate the buffer solution with 0.1 N HCL (30ml) for a period of 60 minutes at the rate of 0.5 ml per minute. Note the change in pH at every 2 min interval for soluble buffers

0 10 20 30 40 50 60 700

2

4

6

8

10

12

160.6 TSP + MgOH2 150.3 mg

160.6 TSP + MgOH2 150.3 mg

Time in minutes

pH

pH time curve of various buffers

Proceedure : In a beaker take 210 ml of water .to this add 9.6 ml of 0.1 N HCL and aalow to stirr with magnetic stirrer(basal stomach fluid)measure the initial pH (about 2.4),add the required qty. of buffer as determined by pH neutralizing capacity of different buffers.Titrate the buffer solution with 0.1 N HCL (30ml) for a period of 60 minutes at the rate of 0.5 ml per minute. Note the change in pH at every 2 min interval for soluble buffersand 5 mint. Interval for insoluble bufferes.

Observaton: As per the tested formula and patent of azithromycin 2g suspension

TSP + MgOH2Wt. taken = 350.1 TSP + MgOH2 250.1 mg Initial = 2.4 pH

Time 160.6 TSP + MgOH2 150.3 mg0 11.45

10 11.0920 10.1430 9.4440 9.0150 8.6860 8.52

0 10 20 30 40 50 60 700

2

4

6

8

10

12

14

160.6 TSP + MgOH2 150.3 mg

160.6 TSP + MgOH2 150.3 mg

Time in minutes

pH

In a beaker take 210 ml of water .to this add 9.6 ml of 0.1 N HCL and aalow to stirr with magnetic stirrer(basal stomach fluid)measure the initial pH (about 2.4),add the required qty. of buffer as determined by pH neutralizing capacity of different buffers.Titrate the buffer solution with 0.1 N HCL (30ml) for a period of 60 minutes at the rate of 0.5 ml per minute. Note the change in pH at every 2 min interval for soluble buffers

0 10 20 30 40 50 60 700

2

4

6

8

10

12

14

160.6 TSP + MgOH2 150.3 mg

160.6 TSP + MgOH2 150.3 mg

Time in minutes

pH

pH time curve of various buffers

Proceedure : In a beaker take 210 ml of water .to this add 9.6 ml of 0.1 N HCL and aalow to stirr with magnetic stirrer(basal stomach fluid)measure the initial pH (about 2.4),add the required qty. of buffer as determined by pH neutralizing capacity of different buffers.Titrate the buffer solution with 0.1 N HCL (30ml) for a period of 60 minutes at the rate of 0.5 ml per minute. Note the change in pH at every 2 min interval for soluble buffersand 5 mint. Interval for insoluble bufferes.

Observaton:

TSP + NaHCO3Wt. taken = 160mg TSP + NaHCO3 350 mg Initial = 2.4 pH

Time 160mg TSP + NaHCO3 350 mg0 8.27

10 7.1420 6.9230 6.7640 6.650 6.4360 6.27

0 10 20 30 40 50 60 700

1

2

3

4

5

6

7

8

9

160mg TSP + NaHCO3 350 mg

160mg TSP + NaHCO3 350 mg

Time in minutes

pH

In a beaker take 210 ml of water .to this add 9.6 ml of 0.1 N HCL and aalow to stirr with magnetic stirrer(basal stomach fluid)measure the initial pH (about 2.4),add the required qty. of buffer as determined by pH neutralizing capacity of different buffers.Titrate the buffer solution with 0.1 N HCL (30ml) for a period of 60 minutes at the rate of 0.5 ml per minute. Note the change in pH at every 2 min interval for soluble buffers

0 10 20 30 40 50 60 700

1

2

3

4

5

6

7

8

9

160mg TSP + NaHCO3 350 mg

160mg TSP + NaHCO3 350 mg

Time in minutes

pH

pH time curve of various buffers

Proceedure : In a beaker take 210 ml of water .to this add 9.6 ml of 0.1 N HCL and aalow to stirr with magnetic stirrer(basal stomach fluid)measure the initial pH (about 2.4),add the required qty. of buffer as determined by pH neutralizing capacity of different buffers.Titrate the buffer solution with 0.1 N HCL (30ml) for a period of 60 minutes at the rate of 0.5 ml per minute. Note the change in pH at every 2 min interval for soluble buffersand 5 mint. Interval for insoluble bufferes.

Observaton:

TSP + TrisWt. taken = 160mg TSP + Tris 250.7 mg Initial = 2.4 pH

Time 160mg TSP + Tris 250.7 mg0 9.26

10 8.3620 7.9530 7.5640 7.0750 6.5660 3.44

0 10 20 30 40 50 60 700

1

2

3

4

5

6

7

8

9

10

160mg TSP + Tris 250.7 mg

160mg TSP + Tris 250.7 mg

Time in minutes

pH

In a beaker take 210 ml of water .to this add 9.6 ml of 0.1 N HCL and aalow to stirr with magnetic stirrer(basal stomach fluid)measure the initial pH (about 2.4),add the required qty. of buffer as determined by pH neutralizing capacity of different buffers.Titrate the buffer solution with 0.1 N HCL (30ml) for a period of 60 minutes at the rate of 0.5 ml per minute. Note the change in pH at every 2 min interval for soluble buffers

0 10 20 30 40 50 60 700

1

2

3

4

5

6

7

8

9

10

160mg TSP + Tris 250.7 mg

160mg TSP + Tris 250.7 mg

Time in minutes

pH

pH time curve of various buffers

Proceedure : In a beaker take 210 ml of water .to this add 9.6 ml of 0.1 N HCL and aalow to stirr with magnetic stirrer(basal stomach fluid)measure the initial pH (about 2.4),add the required qty. of buffer as determined by pH neutralizing capacity of different buffers.Titrate the buffer solution with 0.1 N HCL (30ml) for a period of 60 minutes at the rate of 0.5 ml per minute. Note the change in pH at every 2 min interval for soluble buffersand 5 mint. Interval for insoluble bufferes.

Observaton: Upon addition of acid at every interval pH goes down to 8.9 and regains back within 2 minutes

Tris + MgOWt. taken = 250mg Tris + MgO 170 mg Initial = 2.4 pH

Time 250mg Tris + MgO 170 mg0 10.47

10 10.4720 10.430 10.4140 10.3850 10.3660 10.35

0 10 20 30 40 50 60 700

2

4

6

8

10

12

250mg Tris + MgO 170 mg

250mg Tris + MgO 170 mg

Time in minutes

pH

In a beaker take 210 ml of water .to this add 9.6 ml of 0.1 N HCL and aalow to stirr with magnetic stirrer(basal stomach fluid)measure the initial pH (about 2.4),add the required qty. of buffer as determined by pH neutralizing capacity of different buffers.Titrate the buffer solution with 0.1 N HCL (30ml) for a period of 60 minutes at the rate of 0.5 ml per minute. Note the change in pH at every 2 min interval for soluble buffers

Upon addition of acid at every interval pH goes down to 8.9 and regains back within 2 minutes

0 10 20 30 40 50 60 700

2

4

6

8

10

12

250mg Tris + MgO 170 mg

250mg Tris + MgO 170 mg

Time in minutes

pH

pH time curve of various buffers

Proceedure : In a beaker take 210 ml of water .to this add 9.6 ml of 0.1 N HCL and aalow to stirr with magnetic stirrer(basal stomach fluid)measure the initial pH (about 2.4),add the required qty. of buffer as determined by pH neutralizing capacity of different buffers.Titrate the buffer solution with 0.1 N HCL (30ml) for a period of 60 minutes at the rate of 0.5 ml per minute. Note the change in pH at every 2 min interval for soluble buffersand 5 mint. Interval for insoluble bufferes.

Observaton: Upon addition of acid at every interval pH goes down to 8.9 and regains back within 2 minutes

NaHCO3 + MgOWt. taken = NaHCO3 350mg + MgO 170mg Initial = 2.4 pH

Time NaHCO3 350mg + MgO 170mg0 10.2

10 10.1920 10.1930 10.1240 10.0850 10.0160 9.86

0 10 20 30 40 50 60 700

2

4

6

8

10

12

NaHCO3 350mg + MgO 170mg

NaHCO3 350mg + MgO 170mg

Time in minutes

pH

In a beaker take 210 ml of water .to this add 9.6 ml of 0.1 N HCL and aalow to stirr with magnetic stirrer(basal stomach fluid)measure the initial pH (about 2.4),add the required qty. of buffer as determined by pH neutralizing capacity of different buffers.Titrate the buffer solution with 0.1 N HCL (30ml) for a period of 60 minutes at the rate of 0.5 ml per minute. Note the change in pH at every 2 min interval for soluble buffers

Upon addition of acid at every interval pH goes down to 8.9 and regains back within 2 minutes

0 10 20 30 40 50 60 700

2

4

6

8

10

12

NaHCO3 350mg + MgO 170mg

NaHCO3 350mg + MgO 170mg

Time in minutes

pH

0 10 20 30 40 50 60 701.50

3.50

5.50

7.50

9.50

11.50

13.50

Comaprative pH time curve of different buffers

Tris 1002.5 mg

TSP 160.5 mg

CaCo3 280.4mg

MgOH2 150.7mg

NaHCO 3 350mg

MgO heavy 170.9 mg

160 TSP + 170.1 MgO

160 TSP + CaCO3 280.4 mg

160.6 TSP + MgOH2 150.3 mg

250 TSP + MgOH2 350 mg

160mg TSP + NaHCO3 350 mg

160mg TSP + Tris 250.7 mg

250mg Tris + MgO 170 mg

NaHCO3 350mg + MgO 170mg

Time in minutes

pH

1 2 3 4Time Tris 1002.5 mg TSP 160.5 mg CaCo3 280.4mg MgOH2 150.7mg

0 8.62 7.83 8.17 10.0310 8.49 6.46 7.84 9.8420 8.39 3.02 7.74 9.6930 8.29 2.2 7.63 9.6140 8.2 1.98 7.6 9.4550 8.12 1.84 7.55 9.2560 8.04 1.74 7.3 9

Selected buffers short listedS.No. Buffers

1 MgO 170 mg2 MgO 170 mg + 160 mg TSP3 MgO 170 mg + NaHCO3 350 mg stable combination4 MgO 170 mg + Tris 250 mg5 TSP 160mg + MgOH2 150 mg6 MgOH2 150 mg

Buffers finalisedReason Once the formulation ingested the soluble buffer dissolves immedieately and shots up the pH of the medium

and then the insoluble buffers actes as scavenger to protect for longer time period once by continuouselyreacting with secreated acidso the combination of soluble and insoluble buffers are mandatory

Titration conducted to acertain the amt. of soluble buffer required to neutralize the 30 mints acid 24.5 ml 0.1 N hcl bcs. The soluble buffer should neutralize the stomach pH immediately to 8 and the insoluble buffer shall maintain for next 30 minutesbuffer amount required to bring ph 8

1 TSP 350 mg2 Tris 500 mg3 Na2HCO3 350 mg4 Mgo 120 mg5 mgOH 150 mg

Selected final buffers

1 2 3 4 5 6 7

0

1

2

3

4

5

6

7

8

9

10

11

12

13

14

Selection of Buffers by estimating the acid neutralizing capacity of various buf-fers

Tris 1002.5 mg

TSP 160.5 mg

CaCo3 280.4mg

MgOH2 150.7mg

NaHCO 3 350mg

MgO heavy 170.9 mg

160 TSP + 170.1 MgO

160 TSP + CaCO3 280.4 mg

160.6 TSP + MgOH2 150.3 mg

250 TSP + MgOH2 350 mg

160mg TSP + NaHCO3 350 mg

160mg TSP + Tris 250.7 mg

250mg Tris + MgO 170 mg

NaHCO3 350mg + MgO 170mg

Time in min.

pH

1 2 3 4 5 6 7

0

1

2

3

4

5

6

7

8

9

10

11

12

13

14

Selection of Buffers by estimating the acid neutralizing capacity of various buf-fers

Tris 1002.5 mg

TSP 160.5 mg

CaCo3 280.4mg

MgOH2 150.7mg

NaHCO 3 350mg

MgO heavy 170.9 mg

160 TSP + 170.1 MgO

160 TSP + CaCO3 280.4 mg

160.6 TSP + MgOH2 150.3 mg

250 TSP + MgOH2 350 mg

160mg TSP + NaHCO3 350 mg

160mg TSP + Tris 250.7 mg

250mg Tris + MgO 170 mg

NaHCO3 350mg + MgO 170mg

Time in min.

pH

5 6 7 8 9NaHCO 3 350mg MgO heavy 170.9 mg 160 TSP + 170.1 MgO

8.54 10.66 10.74 9.48 9.888.28 10.61 10.65 7.4 9.38.08 10.59 10.69 6.98 8.868.01 10.57 10.67 6.75 8.567.84 10.56 10.57 6.59 8.647.55 10.54 10.54 6.47 8.536.68 10.5 10.38 6.44 8.43

stable combination

Once the formulation ingested the soluble buffer dissolves immedieately and shots up the pH of the mediumand then the insoluble buffers actes as scavenger to protect for longer time period once by continuousely

so the combination of soluble and insoluble buffers are mandatory

Titration conducted to acertain the amt. of soluble buffer required to neutralize the 30 mints acid 24.5 ml 0.1 N hcl bcs. The soluble buffer should neutralize the stomach pH immediately to 8 and the insoluble buffer shall maintain for next

160 TSP + CaCO3 280.4 mg

160.6 TSP + MgOH2 150.3 mg

1 2 3 4 5 6 7

0

1

2

3

4

5

6

7

8

9

10

11

12

13

14

Selection of Buffers by estimating the acid neutralizing capacity of various buf-fers

Tris 1002.5 mg

TSP 160.5 mg

CaCo3 280.4mg

MgOH2 150.7mg

NaHCO 3 350mg

MgO heavy 170.9 mg

160 TSP + 170.1 MgO

160 TSP + CaCO3 280.4 mg

160.6 TSP + MgOH2 150.3 mg

250 TSP + MgOH2 350 mg

160mg TSP + NaHCO3 350 mg

160mg TSP + Tris 250.7 mg

250mg Tris + MgO 170 mg

NaHCO3 350mg + MgO 170mg

Time in min.

pH

1 2 3 4 5 6 7

0

1

2

3

4

5

6

7

8

9

10

11

12

13

14

Selection of Buffers by estimating the acid neutralizing capacity of various buf-fers

Tris 1002.5 mg

TSP 160.5 mg

CaCo3 280.4mg

MgOH2 150.7mg

NaHCO 3 350mg

MgO heavy 170.9 mg

160 TSP + 170.1 MgO

160 TSP + CaCO3 280.4 mg

160.6 TSP + MgOH2 150.3 mg

250 TSP + MgOH2 350 mg

160mg TSP + NaHCO3 350 mg

160mg TSP + Tris 250.7 mg

250mg Tris + MgO 170 mg

NaHCO3 350mg + MgO 170mg

Time in min.

pH

10 11 12 13 14

11.45 8.27 9.26 10.47 10.211.09 7.14 8.36 10.47 10.1910.14 6.92 7.95 10.4 10.199.44 6.76 7.56 10.41 10.129.01 6.6 7.07 10.38 10.088.68 6.43 6.56 10.36 10.018.52 6.27 3.44 10.35 9.86

250 TSP + MgOH2 350 mg

160mg TSP + NaHCO3 350 mg

160mg TSP + Tris 250.7 mg

250mg Tris + MgO 170 mg

NaHCO3 350mg + MgO 170mg