tissue tests as indicators of mineral nutrition of cotton · with titaaa tests as msei w searsttjit...
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![Page 1: Tissue tests as indicators of mineral nutrition of cotton · With titaaa tests as msei W SearsttJit aa iavestigater is atol® t© iataraia® a "low ar fei#i sapflyi ar a itat® of](https://reader035.vdocuments.site/reader035/viewer/2022071514/61358afc0ad5d20676477197/html5/thumbnails/1.jpg)
Retrospective Theses and Dissertations Iowa State University Capstones, Theses andDissertations
1950
Tissue tests as indicators of mineral nutrition ofcottonHoward Ernest JohamIowa State College
Follow this and additional works at: https://lib.dr.iastate.edu/rtd
Part of the Agriculture Commons, and the Plant Biology Commons
This Dissertation is brought to you for free and open access by the Iowa State University Capstones, Theses and Dissertations at Iowa State UniversityDigital Repository. It has been accepted for inclusion in Retrospective Theses and Dissertations by an authorized administrator of Iowa State UniversityDigital Repository. For more information, please contact [email protected].
Recommended CitationJoham, Howard Ernest, "Tissue tests as indicators of mineral nutrition of cotton " (1950). Retrospective Theses and Dissertations. 13872.https://lib.dr.iastate.edu/rtd/13872
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fissm TESfs m isbigatoms
Of Mimil, lOfllflOl OF C^ff01
Howari Joltaa
A DisseTtatioa Smfeadtted t© tii«
Oraiuat® faemlty ia Parti®! of
til® K#q,mlrement8 f©r the »0gr«« ©f
DoefOR Of pHiiosoMr
Major SmijJeets Plaat Piiysiolo'iy
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Iowa Stat# Ooll«gt
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Signature was redacted for privacy.
Signature was redacted for privacy.
Signature was redacted for privacy.
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©#'t©f«iaatloa ©f altrate aitwgea»##•,. i Wk0SpMrmg i«t«miaation*,.•.•#»»»*••• • 10 Potasfl«a itttmiaation.*««,•##*»#••... • 11
d#-|«yiBlaatlon IS 0&i@lw d e t « i ® i ] i a t ! # & • • • 4 . 1 3
flMit IsTdStigatloa, flie S«l«@ti<im'ef Saapliag fisTO«*15
S«0©ai Iat«»tigati.@a." ' • ' Stmiy*<,•••.••••••»««#*24
ttlari lawitigatiom.' fi#ld Studr. 40 f©nr-t& lamBtigmtiea* ^Sreeiihous®
S tmty 45
Biseiissioi MB- mMOmsiOMS^ 58
SWEIST 6 ?
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IlflODtCflGf
The rapit ®a:p®msl#a ©f tto# ma® Qf ftrtillzer® hm mi®
it that ©xistiag iate ©a %k9 aiatral req*iir@m@ats of
til® ©ott0a plaat' tr# far tmm a<i@tuat«, Tissm# tests Msv«
hm& appli«i t© mm irai'len® trmet #r©ps mi, liaT® affori-
©i a mmM of ®«tiaatia,g tb® »*iti»ittoaal statms ©f the plaat,
Th& .appli#ati@a of saita.ibi« tissme t®st« to ©ottos 8homl4 b®
@f similar falii#, . first ptoas® ©f tlii® prograa was the
8®lfi@ti©a of pwfdi? «sli«aieal a#tli®ts m€ th« a«t«riii.aatioa
of tite pertioa ,©f plast t© b® ffae relatioa ©scist-
iag he'^mn tfe® aTailaMliti* ©f a etrtaia ©leawt or «ltment»
t© %k9 plaat aaa tii« ©eattatratiua ®f tl©s© ®l««iits in tb#
mpmtmmt&X %iM»m .amst fe© kaewm, fli© p®8»i%ilit|' of iat«r-
aftioas lt#tw®®a amtriaat ©leatats aai tMe laflm,®ae® ©f saefe
iateraetioas ©a tk® l#v®l ©f ®l«ffl«ats im tb# tlsfw being
testti la iMpertisat la the efalmatloa ©f resmlts tmm timum
teats, •
Am attempt Ms b®«a mai# to eorrelatt jieM. with tM«
©oae«atrattdja of •a*iti»i«»t- tl«a®ats la thm «p®rim«atal ti«»w®
io am effort to ««tabll»& a mmm9 wbiefa w©iiia be of Talrn#
i® •obtaiaiag •eaflr ©.st^laet# ©f fr©am«tiTit|' as well as ©f
amtyieat i®fi0i®tt«|f.
It is topei that the rssmlti ©f tli« testi aait will
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2 #
fmafaisli basi© iaforaatiea ipoa tii© mimml amtritioa ©t
©©ttoa plamt aoA tiiat ?©r ® «©r® «fflot«at
of aay fee fossitel®*
9
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3»
gIfllW OF LlflllTOll•
fli® tis»m tests i#T®lop«4 at fmrto® falTersity ar«
btisg us«i im dlftga©s#s ©f m%Timt gafieieaoies in com
|8|14,lf ,gl) • fhes# te«ts &m ••«i»twaatitatiT®
tests** aad teT« tfe«ir Msia la tli® mmmpt of ••ejpitioal
Melstei ilO) state® that tay tmiek
test whlefei aeasmrss allia«©ts im ArofS Is tmfejeet t© larg#
mvmiTB* fM® Pifdu® ti®sm® tests# hmmeT,- Mm proTta their
ifalm© la i«t«Miialag tb® iiffertae© ifeatwt©® « .TOffieitnt aad
a«fi«i«iit a»ttiat of altr©g©a, or potassiwi ia
til# tlssmta m&mm&A* ImM iZl) iitilia«€ tlit Fiiifdtt# ti»8af
tests on ©era gr®WM ©a r&jeima %jpm@ ©f ladiaaa soils« Tlie
e©me®atratl©a of tlit fl®a®iit» ia t&® e®r.ii statt vari®<l wltfe
the SQil typt aaS past treataeatj; towavtr, the altaeat
liaitiag ^rmth %e S«t®ot®i im m®st ©aa®8.
Aeo©r€iiig to Sofftr 18} thm ls©st tii^' to mte tests ©a
@©ra is wli®ii tl® flamts a,i?© beariag mm m& are w«ll al©og-
in aaturitj* 1« states that th® greatest iateriretativ®
mlm® la at th# ®ai' of tht irowiag stasoa, ©in©® syaptoma
of aalmmtritioa r«aoli thaly aaxiaw ®xpr«8si©n at that time.
fh« T«lm« ©f isf©»Bati©ft oibt®ia@a this lat« ia th« smmom
is li»itei W th® tm% that mmmmrm eanaot h«
tak®t matil th® folluwiag irowiag s#as©tt«
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la the .Parte# tissii# t«»ts, altp©g©a ani pli®spli©ptts
are fi«t©»ia©4 ilrtetly hf applisatloa of tb« reagemts t©
tto.® split »ra stalte. fotasslam is aeterMlaefl iaiireetlj
fey a a@a»»© ©f tii© aaeaat ©f imm ®eei»iilat©i. la the
tissmt* ffetr® is a atgative 0orr«latl@a. lj©tw©#n ir©a aeetma-
latiett &a& p0tas®i*» mmmts^rntiQm @f '©©ra plaats grown
m #®rtaia s©iX ti'pas.
a©ff®r i&) a©t#i a 4lr®et r®iati0asliif 1|«tw«®a ©aleita
appli®4 at rates ©f ©n® t© foar t©ai aa aer# aad irea adeama*
latiaa ia th© aatal tissa® @f tM mm stalk* fhis r«salt
«a®®st» aa immtB® r@latioa eelsiam aad potassiaa
afeaorptlea* Sa©li iattraotioas are impartaat ia th# iaterpr®-
tatioa ©f resalt® ©fetaiaei fr®* tissa® tests#
S©ars«t& 114) <jriti@is«i ta« as# of s©ii tasting
sfstoM for S#t#raiaati©a ©f plaat aatrltat aaets. I® states
that tlie «fe0ai©al ©xtraetien af aatrleat altaeats froa soils
is aot a aeasw® at ttoeir availal>illty to plaats* fa©
tiT©rg«a®« ia sarfa®« aa4 safe-soil sa4 ta@ reipeas®® ©f crops
t© sail «aatitl@ai ar« tea ir«rlei» aaA r©salts from saoli
tm%& saomli fee iat«rpr«tai with ta®ie li»itati®as ia aia^#
With titaaa tests as msei W SearsttJit aa iavestigater is
atol® t© iataraia® a "low ar fei#i sapflyi ar a itat® of
fealaaea, of aitrftt®.# pbaspliatgt, aai patasli at ma sarly stag®
of plaat grawta,»»
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s.
witbia tb.© piaat teiiied to d«erease with, applieatioas of
aitrogta. fMf also ofes«rf«d as iavers® relatisa toetweea
petassim ami aitrogea..
fyatr, Us J aai ''Tyatr aad Wtbfe il9) working witk cora,
aatt Itaf aaalys©# m aa aid ia i«t#riilalag th© relatioa
lj0tw®«a yi@M aai atitrieat toalaae®, fii®y analyzed tli© sixtii
leaf of the maia at®a aai tb® data eolleeted p®fl#0t®d ia
fea«»l th« aatrieat atatus of tM® plaat. Applieatioa of
aitpogta to tb® soil depressed IM aoommlatioa ef potassiam.
HeaTy appll§ati©as ©f potasJi a@«®ei to h.av« a similar @ff®ot
©a aitrogea aeeamalatioa. Pkospiiarms did a©t alter ta# ait-
rog«a or pO'tassiaa eoaetatratiea ia tli#ir aaalyses, aor did
the latter two «l«eatf baf® aay tffeat apda pMsplioras
aeeaaalatioa, la tliig experiaeat tatal aitragea, phospliorms,
aad potasiima wer® dattraiaei. fyaar and Wefelb foaad tha
eritieal lav©Is for aitrogaa, piiesptorms, aad potasiim ia
th® sixth laaf of a e©ra plaat dariag sillciag to b© g,90',:
.gfS, aad 1,30 par ©#at raspaetivaly,
loaert (§| mtilizad plaat tiasaa tests a» a gaid© ia
fertilizing taaatoes. He ooaolmdes that the solabla
aatriaats give mora iafematioa in this raspaat aad that
a total aaalysii oftaa ohsearet iaportant variatioas ia
th« amtriaats ©oae®ra®d» fh© aaaatatratioueof solahl®
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f,
mltrog«n phosphorus la matiaip® petlol©® at eritioal
stages of growth war® <ltt«irals®5. lM®r tbt ©oaiitioas
®f his ®xp#rim«at inriag laj |t©ap©ratttr@ 70®), toaate
plamti iMemli •eoatata aboTe 1000 fpa. •©# solmfelt altro-
g«a aafl gOO pfa, ®©lmtel« pli©sfh©rms. UMriag frmitimg
tli« altr©g«B e©a©@Jttratloa ae@r©as@s hut gbOTW aot €r©p
h9lm 500 ppa, Piiosfherms sbomli iaorease t© 40'0 ppa.
Mt®r th® asttiag of frmit tfc# aitrog#® ooaotatratlon
sliettM iaereas# to mbme ISO© ppa, if th® plants art
ai«t'«a^®3LF sttfpli«a with this «l®a#iit.
llrieh iZQ) «»pl©3r«i tissw tests as a a®aas of
diagotsiag awtrieiat defiei^aeiei ia plaat tissme®. Ia
g^'apta, th© petiolsi w®r# f®mai to. show gr®6t«p rangts
ia petaflsiia e^aeeatiratieii with tr«at®eat thaa th« leaf
hlai«i h©w®ir@r, with t©iMt©@«, this aitmatioa was p«-
v®rs®i aai th® biaiea w©r« mom mttfal as a. a®an# ef
iiagassiag aatriemt i»fi0i©a«i«.i .•
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9,
impQTtmt* la of this, 'etomieal nstiiois wer®
atoft®i AI0I1 wo«Xdi tli© itt@Miiaatl®a ®f a large
a\aife®r of sm^XeB ia a relativtly shoyt tlm® witfa a
alaiatm smrifim la tetiiraei'#
0fe,«aieal Metk©4»
ef aitret® aityoaea
l#ag«at®i
I. iytitiae .^iipochloyiie ia Ig©. Solmtioaj- ©issolT®
4 ©f l)3m«ia« hydrH'tJilorii# 4a water aM fiilat® t© 10-0 ml.
g. l©rgaa*» txtpattlag solmtioas Bissolv® 100 m*
of ioiiia aeetttt® ia SQ© *1# l^Q, aM 30 ml* ©f
glaoial ae®ti© aeii «ii teriag, wlwi® t© ©a® liter,
3, e©a0#atratei
A Bllgat soiifieatiea of ta© hm&im® a«ta©4 as oat-
lla®4 fey Pi»«ii aai lagllsto. fl§) wai as#i, f© a ssaall
aliqaot ©f 'ta® «il«ar®i ©xtraet (mmllf 1 iil.»| aaffi©i«nt
,M©rgaa's iolatlea III) was aidei t© teriag tht irolum© t©
§ ml. Oa® »illllit«r ©f teaelae liyipoefalorli® s©lmtioa
aai § al. ©f IgS©^ ir®F» aiiei ear#fmlly. fie saajplts were
r©tatet »l@irly aa€ tliea all©w®d t© ataai tea miamt®® oa a
0t®iim fettit, aatll tli© y®ll©w e@lor 4®T®l©f®i.. Aft®r
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10.
toollflg tor fir® aiamtea, tlie of th@ eolor was
with a El«tt-STOa®3?s©a ptoo1jo@l«0trl© celorlaeter,
®ai tlie aaomiit ©f aitrat® ealemlatei fwm t staadar4 ourf©
ma m tb© us®® imitymeat# A 11m# filter #480 was eaiploy-
@i. litrit® aitr©g@a is iaelmdei in tMls ttstj hmer®Tt a«
th« aaottat of nitrite aitr©g©a witMn t&« plaat is verr
l©w, tMs is net ©•Qaii<S«rei a striems error.
gteQapliiarus i«t®gniBatiem
Isagtats I
1# toeaeaim i»lyMat« solmtioas Ditsolve g.5 gjo.
of afflBeaima ffiolfteaate is imffieieat i 1 %S04
t@ feriag ths volna® to im al..
g. Amlaom-apMlielsttlfoai® aeii iol*iti©»j Bisaolv#
3 gffis, of soiiw Msmlfitt la ZQ ml. of HgOj to
tMi S0lmti®m ait ©.l pa., l-amia©-g-aaptii0l*
4--stilf®Bi0 aeii, aai m* »diwa salfit®,
Briag tli® v&lwm to ItO al. St©r@ la a Urowa
glass ijottl®.
a#tti©i for pfeOBpkorms Setarmlaatioa ^ai@4 was
ifeat giir#a toy Wolf To a S-ml. alit»t of tao plaat
ertraet, aM g ml. of aramoaiaa aoljMat# aai. 1 »1. of tli#
aaiaoaapbtliolswlfoaio aeit soltitioa* Miae th® eoateats by
rotatiag aad allow tea alamtas for tlie color to i®v®lop.
![Page 17: Tissue tests as indicators of mineral nutrition of cotton · With titaaa tests as msei W SearsttJit aa iavestigater is atol® t© iataraia® a "low ar fei#i sapflyi ar a itat® of](https://reader035.vdocuments.site/reader035/viewer/2022071514/61358afc0ad5d20676477197/html5/thumbnails/17.jpg)
n.
l«ai i® tM© ©©loriaeter wsiag a him #4gQ filttr. Slue®
thim tm&img fellows Law fe®twe©a on© ami tw®lv®
pfii. ©f fliospM®rm» Cfossibly kiglier), tlie eoa«®at3fati©as
®f til® alitmots ©aa €«t«3mia®i readily by tii® «s© ©f a
staadaifi saiipl® aai tli# ealettlatleii of a faetoa?. tkus it
1» not a«e©8fary to plot a ataMart emrr®,
eeae.., Sti. ^ mmrn Sil. " ,
fa©tor 3s, reaiiag ©f mnkaowa s ©©a®, matoowa.
fotaaiiia g»t#gmiaati®a^
laag«at0!
1, Soiiw fi©tealtiaitrlt® ®©lmti®a{ I51ss®lir« i,,92
g»8. &f mMm. ©obaltlaitrita ia watar aai,
iilwt® to 100 »1# Stsr® ia a hrmm glass-
atejpparai bottla at §®0-#
8» Is0propyl ale©li©l anfi femaliahyS® solmtloat
f© aia© parti ®f l®®fropyl al©0h©l, aM, ©a®
part ©f for»aMt&yfl@.
3, M©rgaa*s extraetiag s®lwti©fli Llatei miar
tii« aitrata aitrogaa t®st»
4, arable, ©a® par ©aat ia HgO#
a© p>ta8»im jpr0®«S«r® ia assiaatially ttot givaa by
Wolf (82) with a sli^t ao4ifi®ati©a. f©«r millilltars
![Page 18: Tissue tests as indicators of mineral nutrition of cotton · With titaaa tests as msei W SearsttJit aa iavestigater is atol® t© iataraia® a "low ar fei#i sapflyi ar a itat® of](https://reader035.vdocuments.site/reader035/viewer/2022071514/61358afc0ad5d20676477197/html5/thumbnails/18.jpg)
u,
of «®rgaa'« ©xtraetlag solution are aaa©4 to a 1 »!•
aliqm«st« fiift saaplts ar# tbea eeeled to l©®e. FiT«
alllilittrs ©f 01301 BQilws. tolJaltiaitrit© aad 4,5 al.
®f Isopi-Qfyl aleoliol soltttioa ar® tlita aided. It
wai f©iuftd tliat tli® iMlmsiea of §,2§ al# of th® g«m arable
jselmtioa tM peroipltato, sa»j>lts w®r®
eartfttlif alxdt sad tmrbidiattfit B«aiur©a@ats mad# «siiig
a #§S§ ia the ©olerimetei?,
Magae«iTO ieteyaiaatiQm
l«sg«atss
1, • fitaa |-®ll©w, 0,10 p®p m&% is 1^0, flia eo^etmd
©btalaed fr©a 'tli# Allied 01i@aieal aad B|"« Corpo*
• ratioa prowd •atisfaet©rr*
•• g, Ilytp#x3rl«ttte lijdr0olil©rid«,' fif® per m&t ia HgO.
i, Seiiw lijdr©3£id«, g.S
4. e©af9a«tiag mlntimi UisaolT® 4,4 g®, of oaleiuit
a®«tiit© bM O.Sf ia 800 ,iil,
HgO., Mi, 10 ^al, #Qa©, ^H01 miai dissolw t&« abor®
fultSi thsQ. dilttte t& ©a® lit«r witli V-
5, Starofe a©Xmti&ni Diesolv® S gm, of solttble stitreli
ia 1©0 alt. ©f tot water, Filttr "^is s©lmti©a
wMl® toot,
i, starefe r®ag®ati Itmal vel«M#s ©f tto« e©»p«a»«tiiig
![Page 19: Tissue tests as indicators of mineral nutrition of cotton · With titaaa tests as msei W SearsttJit aa iavestigater is atol® t© iataraia® a "low ar fei#i sapflyi ar a itat® of](https://reader035.vdocuments.site/reader035/viewer/2022071514/61358afc0ad5d20676477197/html5/thumbnails/19.jpg)
13.
soimtlQa €a<S the stareM
f@ a 4 ml* alitmetj S al» ©f HgO, 0«SS ml# ot %li®
MrAr©3crl«iat ltF4r®eltl»M« solmtlcia aai 1 al, of
stai-ea r©ag«at ay© ai4®-i aai tl» simplBS tfe®i«©iig]^ly mixed
(131. Mi on# milliliter ©f titaa j&llm aM agaia'mix.
Smffieitat laOl., 1#B§ Ml#, is tiiei to i«Ttl©p tto,® mag-
ii®»iwa lafi:©. a© samjples ar« ©®ap®r®i witi a tmitaMe
®taa4ari mrw-^ mlm ® grmm, §MQ filter la tto@ ©©lor-
i»®t®r (IS),
Calgiw teteraiaatiQa
l#ag©at«.s
1. S©ap 8©lmti©ai DIssoIt# 0,®0 m* atemric.acid
IrttrFstallisfi) al. ol®i# aeM Cw.S.P,)
ia Sg© al, of 95 per m&t ©tMyl^ al@ofe©l l&f li®at-
iag 0m a h&t plate. M4 16 m* mr*
b®aat« «is®®lT®t ia. al* ©f liot figO, Mil tea
aismtts, eool, ait 3i0 al. 95 f©r e«at
al©oli©l, 4 Ml, HgO aafl l.i al. eoae*. OK*
Filter after 34 li©iara aad etor® la a pyr®x,.
gla»s-st®iper®i fe@ttl©.
g. Mm&mMml ©itrate solmti.oai Msselv# l.§ g®.
aoiiia eitret© ia HgO, aM 14 »1, eoaeeatratei
![Page 20: Tissue tests as indicators of mineral nutrition of cotton · With titaaa tests as msei W SearsttJit aa iavestigater is atol® t© iataraia® a "low ar fei#i sapflyi ar a itat® of](https://reader035.vdocuments.site/reader035/viewer/2022071514/61358afc0ad5d20676477197/html5/thumbnails/20.jpg)
14»
tad ferlag tfa® -reXiwe to ©a® liter.
ffe« reageata ar® tii©®® nasi fey P®#0li ajad laglish {13|.
f0 a 3 Ml, allqmet qS tb© plant extract in a aaall Ti.al,
4 al. ©f %h9 aBffltoiiiaeal ©itrat# ©olmtioo ar« added and tlm
emtmta aixed. fki?«© mlllllittr# of tiis s®ap solmtloa
®ri addfd eai fli« lai^lts are allowti t© 'Staad
f©r tiiirty aiamtee, after wtolofe • tttrlidia^trle m©a.smrmeats
ar® mad« mtiag a blwi #480 fi.lt«r ia thm ©oloriattert Th9
mmm%T&%iQm ®f tii# samplts ar« 4@t®r»im«d by ©eapariaoa
witb a staaiard mrte raiding froa *©5 ag, to Q»3 mg, Ga
p®r al#
![Page 21: Tissue tests as indicators of mineral nutrition of cotton · With titaaa tests as msei W SearsttJit aa iavestigater is atol® t© iataraia® a "low ar fei#i sapflyi ar a itat® of](https://reader035.vdocuments.site/reader035/viewer/2022071514/61358afc0ad5d20676477197/html5/thumbnails/21.jpg)
15.
iisnLfs
ft&stm t«ats aip# appliea to r&iom portioas ©f tfa®
piaat, i®|)#a4iag apom %h& ti-sp ia %w©stioa, Th@ Twt&m
t«sts ar® M:i© iir««tXy ©a tli® eofa italk or on small
tlse® m% trm a ]pr®-4®tei!«ia«i zoas ©f tii© stalk. 'Iteaert
werkiag witii twmtees, ©apleyei tfaa petlolts of tli©
l©w@r matar® l®a¥«,s-i feowewy, Vlrl«h (SO) itat©s that ttot#
l®av#8 ©f tbis plaat ar® m»m seasitlv® to nmtpi.eiit oliaagea
aai tlia® glv® a ^@tt©r flutttr® of its amtrl«mt status, ia.®
latter lawstl^tor fonai that the petioles of grafts gav#
tbo ieit r®sfoas« to mriott® l9reln of potassiw. Oarolmi
(1), ftoaas aai Maek: |1S) ami ©tMps feav® loaves from
a dofiait® loeatioa os tibe plant is tk«ir aetkots.
It was mm&Bsmpj at tit© toogiaalmg of tliis projeot to
iot®ralae whi&h portion of th® oottoa plant wouM %« most
gialtafele for ti«®me t##tiflg* Tb,® ssleotion of tto.® tiasme
to h& «iploy®4 woiili flofoai mpoai Cl| ®as« of hi.n41tng,
|2| oonooatratioas of t&® varioM aatrioats witMn th«^
tissmo, CS) rospoaitfi of tho#© «l®a#at» in %hm tissw to
variations in tli« smbstrat# 8t«>ply and |4) e©rr«lation of
mitrioat eonetatratioa# with yioli,
y'irst Invfstigatioa — fli© Soloetioa of Sampling fissuo
Os 3"mn« 20, 194f, Stonovill# g-B upland eotton was
![Page 22: Tissue tests as indicators of mineral nutrition of cotton · With titaaa tests as msei W SearsttJit aa iavestigater is atol® t© iataraia® a "low ar fei#i sapflyi ar a itat® of](https://reader035.vdocuments.site/reader035/viewer/2022071514/61358afc0ad5d20676477197/html5/thumbnails/22.jpg)
16.
pl&at@i ia t&r®« gallon, gltzel pets fill@4 with washed
trttk AfttF geraination, the iseillags w®r@ divided
iato fomr gremps aai amtifieat treatmeats w®p© started,
Oa© liter ©f a half-strength H©aglaad TOlwtioa (7) was
applied to th« first gromp daily. fhi.s treataeat was <soa-
sii@rtd th# high level f©r aitrogta, phosphoras, aad
potftssiim, A s#®©ai gromp was smppliei the »»e^ selnti^a
@xe©ft that th© aitr0g«a was rtidiated te ©a® f©«irth th®
e0a®#atrati©a astd ia loaflaad*® solmtiea. fh© sam® pro-
e®d®r« was asad ia a third aad foarth groap, la gramp
three, photpharaa was redacad to ©ae faarth that of
l®aglaad*s aad ia tmr, petassim was dropped to that
laval# fhis arraagaaaat gava faar iGlatioas, a hasa solatioa
with hii^ It P, aad 1, a law aitragoa, a Im phosphams, aad
a low potasaias solmtiaa. Oa faly 3t tha plaats ware tb.iaaed
to Ihraa par p©t» f© iaaar# th© availahilitj ©f all tha
amtriaats, the plaati war© watered with tap watar ia which
tha pH had hasa adjaated t© S.§ with HiOg Ig). Oa aaeh of
tha fallowiag datai—Aagast 1, 8, li, ai!«l a typiaal
plaat was hervastad fr« tha *hi,^" traa1»tat aad dividad
iato its e«»p©aaat parts. Th® smplea ware aaalyjsad for
solahia aitragaa., phospharas, aad patassiaa aad tha rasmlts
ara praaaatad ia tahla 1* A portlaa of tahla 1- is prosaatad
graphieally ia figwa !•
![Page 23: Tissue tests as indicators of mineral nutrition of cotton · With titaaa tests as msei W SearsttJit aa iavestigater is atol® t© iataraia® a "low ar fei#i sapflyi ar a itat® of](https://reader035.vdocuments.site/reader035/viewer/2022071514/61358afc0ad5d20676477197/html5/thumbnails/23.jpg)
If,
flewerlag startei ©a the t#ntii of lugmst ana it may
fee ©feMffefl tliat tMa oliaag# la th® fbyslelegital aotlTity
of the fltat Is r®fl«0t«d ia f©ae«Etratl©aa of
aitr@g«a, ptesptotrms, nai fotassium ia all tissm®® aaalyz®i
ttot »®ntte..
from tfttelt I aai flgar# 1» it mm 'fe® s®®ii tkat tk® NOg
mttr@gta ia tit© 8t«a la@r®assi ia e©a0#atratloa a« th® sten.
a.p@x was affr©a0h®a. fkd «ia-«teia Isaf l)lad®s w®i?t eoa-
»i»t®atly lew la lOg aitrO'.^^a, wli®r®a« th® »aia-st«ii pstiel®®
w®r@ Mgli ia tlis.t ©@aftitm»at, Maviag a©r« tfciaa 0a® per oeat
il§g aitrogea Ctry might Msial m August I, Masoa aat
FMllis (9) Stat® tliat with thm ®Ba®t ©f flow«riag aueh of
t&® total aitrogaa is a©v«i from tto© ir®g®tatiir® parts of
t&e plaat iat© tfe® fruitiag orgaas, SuoM a aoT®m®at
ii r®fl®ot®« ia tfe« eoaeaatratioa of S©^ aitrofaa also,
a# sHowB la flgmr® 1.
fler® was llttl® differaae® ia tb« ioaaaatratioa of
iolmtolt ptesphorus l>®tw«®a all. fraotioas of tJa® aaia st®a
aad til® »aiB»st®a f®tiol«Si km&mr, tii® aaia-st®* i«af
ijlaSas war® uaifonaly Mgfaer ia tMa ©leaeat at all 4at®s.
H®r®., as ia th® eat® of aitrogea, aa afe.rupt drop la plios*
pMorua is aot®d l>@tw«@a Augast 8 aafl. August IS ia tk®
![Page 24: Tissue tests as indicators of mineral nutrition of cotton · With titaaa tests as msei W SearsttJit aa iavestigater is atol® t© iataraia® a "low ar fei#i sapflyi ar a itat® of](https://reader035.vdocuments.site/reader035/viewer/2022071514/61358afc0ad5d20676477197/html5/thumbnails/24.jpg)
18.
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MSP
1.1 —
1.0
s a ® >1 w .4 o •p •H iz;
3 <D O
u o
•2 MSU 1/3
.1 — / "'"X \ \ MSM 1/3 /+/ \ \ >
MSB \
MSL
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a (D o
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mv \ \ \
\
"TfeU 1/3 MSB Zlx::
I/SM 1/3 MSL 1/3
Aug 8 15 2E Aug 8 15 22 Aug 15 22
Fig. 1. Soluble NO3 Nitrogen, Phosphorus and Potassium in various portions of the cotton plant harvested at weekly intervals. MS, main stem; L, lower; M, middle; U, upper; P, petioles; B, blades.
![Page 26: Tissue tests as indicators of mineral nutrition of cotton · With titaaa tests as msei W SearsttJit aa iavestigater is atol® t© iataraia® a "low ar fei#i sapflyi ar a itat® of](https://reader035.vdocuments.site/reader035/viewer/2022071514/61358afc0ad5d20676477197/html5/thumbnails/26.jpg)
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fabl® g ^
far G®at Selttfel# !©« Whmpkorm and fota»si» ia ;S«rTr®@t®« i«gust 3©| 194?» flfures Llst«i It lew
• • ar#'lf®rag®s ®f f«/leflieatloas*
fr®ata®iit •F#r ©«at i0gi f®T eeat P Pti» ©eat.K
Itili F,.I li&m
•Mi .070 S,M: Itili F,.I li&m «OlS .138 i . l i : lm piiQaplierms ,41f .064 5.73 Lot p&tmmim. •tS4§ ,089 3,5# •
Aaaljsls @f
Sowf-ee far. M • Sg E ' • 1#. lit
nitrogen Total Treat. R©p.^
' •i.rr®!' • f if
1,S74 . ,902 ,108 ,663
^ .SOI ,oa,i ,0as
..,4S
•OS »01 •a44 *194
fMesfli-srms Total Treat, Rep, lrr#r '
m • 3 i m
, 051 ,028 ,007 , o i e
,0095 ,0007 ,0006
mM** • i.2i •
,08 • .01 ,033 ,030
poteeslw Total Treat, Rep, Irror
m § 9 37
47, ©i i7ri§ :4,iO 15,ei
fm ' ,48
,58 • 83 '
,05 . ••01 ,70
••Berntil® a»t®rist, slgmifieaat tt ,tl leirtl.
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gg,
two e,3?lt®3?ls ««t f&rth rnmllm im tMs @a»« ©f
toatliag aai em&mtw&tlm ©f antfitiits faTorabl® f®r
analysis, it was wortto wliil® t® pr®0®®i fmptMey
with t&« iair«stliati©a msiag tli# p«ti®l«s mf ttoe experi-
atatiil p©i?ti©ji Of tb« plaat^.
®m Amgiist Mp »t?®aty-*©a« taji after flaatiag, petiol#
saaplti wer« tate»a fr©a tb« rtmaiaiag plaati. Fetiolfis w®r®
hmrmteA fpoa tkn third eai foartfa a©i@ froa tfe# ap®x of
tk« ml& 8t«»# fli©»« s«apl®s w#r« iriei t@ eoastaat wtight
at sad ftpproxlaatelf i§0 «g, allqmot# w«r« talc«a for
aaalrfis. lewlts of tl# aaalysos ar® glvta la table E
aloag Witt tb,® swmmtj of tli® aaalysit of variaao#.
Iroa tteo m&M Aittmrnm r«t^r@4 for ©igaifioaace,
it apptars as thoa^ tie iiffereao® fe«tw«oa %h® kl0i aai
mm low aitrog'oa tr®.ata«at mm aot sigaifieaat, ik®a
aaalyaiag ©sly tk® Jiigh -rersas tin© low aitrogoa tr®ata®at»,
&»#v©r, ®a f TOla© of It.IS is otjtaiaoS, wbieti is lii^ily
aigaifioaat* fb« aitrato aitrogta ooaooatratioa of the
p#ti@l#«, tM#r«fort, wai low#rei. W « roimetioa ia,tli®
mmm% of 1% ia tb@ aatritat mmAim* A low or liaitiag
gmpply of aitrogoa ia tli© aotiaa taaS'©<i aa aeommlatioa
of solmfelo pkospborms ia f«tiol@». f&is iateraotioa
li.as iota roportoi ia ottoor o.rop8. A kaowleig# of tbis
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of s^lmbX# ftospte@yms ia tli® pttislss 4 la mt iiaialsh'with
a ia ms a© sifaifiiaat iiffertae© is aottS
the &i# aM low piioifbortts »oliiti®a,
fb.« f®tassi» 1«T«1 ia tin® f®ti@l# mri&i with the
m&mt ®»fl@y«i ia tli® aetiaM, lat was mot iaflasaeti fey
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fartiaalar sl«iiiat la th« imfestrat©, hat mr hav® h8«a
asTOtiatsa with a lew ©r limitiag smffly @f »«© athaa?
tl«a®at, fhia iat«raetl©a was sh©wa ia th# hl^ »%
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25.
2.5
Ca
2.0 N K
rH
ii.5
rH
1.0
DAYS AFTER PLANTING Fig. 2. The accumulation of nutrient elements by
the cotton plant. Data taken from Olson and Bledsoe, Bulletin 222 Georgia Agriculture Experiment Station,
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gromfs e©iT»sfoadliig t® aatrleat tyeatmeats
©apl®f@4 la tills lmT®stlgatloa, fM® feas« trealaent o^a-
talii'ti twie® tto® t^aatltlei of altrogsa, ptosflieirm®, p©-
tasslwi,, 0al@lw, aad aagii@slw f#mai la flasts aaa- •
3.ys5«a %f Q1s®b ami (IS),- %km lasmrlag a smffleleat
mpflj &t for go®i grewth, Smlftr was aai»i at
affsj-oxlaattlf tM ®aae at# as ph©spli®ms, Xmu was smp-
pM«t l& the t&m &f pm&emi irm mii-9 ami #ttity trae®
«l®««ta w#r®' mppHM imrlag tl® »«rs® ©f tli® lawstl-
gatloa. fkls fees© trtata®at was i#slgtet«a as tli© '•blgk*'
tir®«tatnt» I\i%'leat apfll#atl®as w«p« #T»rr flftesa
tars aM ttoit qmatltl#s mfpll«i w#3f# 4«t®i"alii®t hr tli«
afes®arptl@a mitfm Bkmm la flgtty# g» Apflleatloas, hosirever,
mmm. aai® tw© w«i»ka la aimaet ©f 1^® 0mrT@» la flgir# Z,
fafel# -i stows %h% tfeli?t®«a tFtatasBts aloag wltit ta« total
as»mnt ©f «l«m«at appllei iaylag %U% mmsm ©f tMs
lair®,stlgatl®a.. fh® flaats w«i?« wat©.?®! aaifefally t® awia
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f«lt tliat tilt #f wat^rlag oaas®i th« piaats to to#
g?wa md#i* 'jfatlity *©istare strtss©»» Iat©a C3| foaa^l
ttoat m%m of wat®'af aapflj t© e®tt®fl gems, at
Sfeaftap, Calif©rala, raiaaai tto# grawtli ter apprexlmatalr
SO fay aaat, afta l&oa#! a© wlltlag ©f tli® laa-res wa»
aotas.
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S7.
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ApfM-ffi C#tt#a flaa^ Imrisg th@ Se-#@ai lmv#0tigatl#a
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Um 5,300 am l,17g 4.56,1 4,174 1,614 Met, ^ §,300 ,iSS 1.17g 4,5ii 4,174 l,ii4 mm " 5.300 i#iii 1.17a 4.ssi 4,174 i,ei4
Urn smlfrnt- i.300 1,116 ,.lt© 4.§si 4,174 1,614 M#i« « 5,300 1,116 ,:g86 4.,660 4,174 1,614 mm « 5,300 1,116 l,m 4,§i6 4,174 1,614
im mleimm S.300 1,116 1,17E ,7ii 4,174 1,614 ««i, « i,300 1,116 1,17S g,g83 4,174 1,614 Mife « S,300 1,116 1,172 4,i6i 4,174 1,614
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li©Wf' a®ga#slm §,300 l.lli 'l#17.i 4,§66 4#174 ,g6t M«a, « i.300 1.116 lafg 4.566 4,174 .807
« i,300 l.lli l.lfS 4,174 1.614
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tmhlm 4
of Tariotts ImtriMit Treatmeats ea aai. Helatiw Irmlt-falJBgss of CottQa Figai^s Qlvea Afegama #f yoag Replieatieas.,
f i O - B a y ^ § 0 - B a y ' " l 4 5 - D a y harrest kayyeat. bary®g% i)ry wt.
g». •wt# ga. "Wt*
m* Height ^resh
wt» Dry wt.
Wwf wt, Mils
lelativ© fruitful-aess-^
aitr@gea mm, « 11^ "
t,f3 3.fS i,15
87.^ 146.5 isg.e
16,6 28.4 24.5
§4.6 •53»0
61.0*» 15.1*'^ 113.8 25,1 114.2 23.4
' 26.9*» 7.38 34.9 5.27 34.4 4.§®
ptesfte®pi@
*
S.90 3.98 3.15
95.8 123.0 132.8
18.f a3,.4 24.5
52.5 5f.l m.Q
m,B** 2#.f 104.8 24,3 114.2 23.4
St.5 30.8 34.4
6.5i 5.25 4.6©
Ijm Mmltwt • *
MEh
.S.*#3 3,4a 3.15
107.8 139.8 138.8
20,5 25..4 24Ǥ
Sf.5 S5,.0 m,&
115.5 112.3 114.2
24.8 23.3 23.4
33.5 28.4 14.4
4.55 4.90 4.i0
Low eal©im Med* » Higfa «
4.38 4.4S 3,li
139.3 133.3 132.8
25.9 23.9 24.5
62.2 55.f 53.®
124.3 109.8 114.2
27.2 24.4 23.4
31.1 32.9 34.4
4.42 4.78 4.60
I^w fotmssim Ket, « ligi ••
4.40 g.fS 3.1S
120.0 121.0 132.8
22.4 SI.? 24.5
61.1 56^3 m,Q
IM.f 105.3 114.2
25.1 23.3 23.4
34.1 35.® 34.4
5.02 5.46 4.60
Im aaga®sim Met. " Siffik ••
s.sa 3.40 3.1S
loa.f 131.3 132.8
It.i 24.1 24-.5
5^ m 5 56.f 53.0
110.0 110.5 114.2
25,0 24.4 23.4
31.8 34.5 34.4
4.20 4.75 4.60
mmber ©f feolls f©r 100 graa® ®f fresfa flaat weight. ••B©«tel© asterisk, sigBifi«aat t© .01 1®t@1.
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30.
%h@ firj &t Mils, TMis &ls® eemsti a
»©tie«alX® y®ll©w.lsg ©f tke f©iiag««' fli« Imt phmwhoruB
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flaati wtoil® the ?®a«iaiag mtwimt kai so
#ff««t mi>©» w#lgb%, QT itpf®araB«®| tlins affortiag
9zmllmt a®t®]pial fm tii« afplteatioa ©f tisstie
fM rtsalis ®f til® aaalF«#-« ©a the p®tlol©s
liairT«at®d at to, to, aaA 14i iay« «« pres«at#i la tal)l«s
5» ®,. lyad f f©»g®©tlir«lr aai ala© la flgmr® $. la, tfa®
@ag« ®f ti® aaalysts oa tJl,® iO»4ay p@ti©l@» (tabl® S|
littl® itgiifitaae® 4m@ t© tip«ata.^t wss ©fetaia®i, feisibly
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ty«atm«at, wMste. it la felt was «aias«i fey th®'a®tfeoi of
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til© pttl©l«s at til# SO-iay tofvest failed t© e©r?®lat«
with tk%iT «mpply la tl® aatostrat®,. with- @mt ^etptloa.
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latl©a fe®tw«»B aitrogea la tli« sm^stmt® aai aolmfel®
plii©spli@rmi ia tto p®tl©l«s. rnsmimmUm &t tl@ pfeoaptoorms
l«v#li im 'ttoi® petl#les ©f th® "meiiim," ani "lii#!"
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31.
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Tissw fmt Eestilta. ©a tvom 60-Bay Harvest ©f Sa-eoM Iav©#tlgati©a#
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freatiaettt Fegeeataae in Bttiele
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Lew pfaespMonii l»SSg .lig 6.to ,§Bf Mea* « 1..44S .Oil §.88 .581 Eigli «• l.if4 .07S 6. &S .473
Jjm snltwt 1.404 .107 §..40 .48g 1.463 .Otl 5.W .518
Mim ** 1,574 .073 6.§2 .473
Lijw ©mlelaa l.m .Ofi 7.44 .588 Met* » l*4"?'f ,.Oii 5.79 .539 Mim " 1.5f4 • 07$ i.S2 .473 ,
Low fotassim 1.473 .088 5.3i .5St 1..501 .087 ©.73 .50g
Hlg& •» i.af4 .073 i.sg .473
JUiw aagiidsl'w 1.883 .102 e.M .519 M®d» » 1.636 .083 S.03 .481 llgii » 1.574 .073 i.§2 .473
^ Emh tlgmm I0 aa avtrag# ©f f©» r«piieati®fls,
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* Sigaifi#sat to .©Si •• slgaifieaat t© •OX XeireX.
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MmU figmr© is «a average @f f©mr r®pli.©ati©ttB»
^ Isiioates sigQifioaat correlation coefficients tostweem th® 6@aoentration of elements in the petioles aM
tfeatment.
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34.
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High Mg
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60 90 nits sc §^5 15? DAYS AFTER PLANTING
Fig. 3. Concentrations of nutrient elements in the petioles as influenced by their level of supply and age of plant.
60 90 145
Substrate
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ii i6—itr
in 4a
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38.
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38,
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aeiinBi was ©e®fi»®€ t© %he ©alaiaa, l®ir«l ®f tli® p®tidles*
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tfe® ^ Ia this Mrvm%t s^strat# magmesim was
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iaiieatiaa ©f altf^aa tafitieaaj was ©tetaiati ia the first
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41*
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f©ti©X@s a«f«!ai«a la a Isrg# mmBwm npom e©m©«iitratloa
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44.
aaa tto® fiitiolf teataat of fajfiotis mutritat ©Itaeats w&m
of Msmits. Igalm %h& ia#gativ«
®0tr«lati©a« petassitta sufpliei m& tiie petiole
©al©im aai aa^esim w«r« ©btmimed# la tk« fotassiaa-
©altiim r®latios., as 9hmm ia taljl@» 8 aai t, m© a©tmal
4iff@r@a©«« ia feti#l® ealoium fe»iagiit afeomt tef f«yti«
limit patassim wm^m soall,. raagiag ia tto© fO-iaf sampling
tmm ,3®f f®j? §m% for tli® 2®^® petasM l®ir®l t© »S3® pet
f«at f®r tto.® fifth 1«T®1 ©f petassiai. TailaMeas ia tli®
p®tlel® aa®a®«im im« t© f®rtilia»r potassim w®r® ©f a
lafg«y ord«"3r tiiaii tMs® ©f ©aitiwa# im iawrs® ralatioa
wa# fomai fe®tw®®ii f©taasi«a aad p®ti©l« 10g aitro^pa. ^lis
sigaifieaiit mgm%ir@ torralatiea was ©btaiatd ia %G%k
saapliagi, A signifieaat iata^s® iat®?aetioa was fomi
for potasfim smjpply-petiol® f^esploftts ia tli® tO-iay harvestj
^©we-reir, tke lg©-aay aaajpliag failti 't© »km meh a r®latioa-
»liip.
fall® 10 ooataims fi®M data ia fom4® p®3f mm of
80«4 oottoa for «afli tr«a'toieii.t, ®©o f®w ofeaarratioaa ar®
afailafel® to mmpuwrn jri«lfi witli patiol®'aitr®gefl» ptooaphorms,
m potasiiw. bo» g®»ralizatioa8t tot©w®t«sr, »®« ©ti¥iou«#
If laio l«Tal ©'f 1% aitroges ia tie fatiolaa is ©o»par«a
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fatel® m
fissM® fes% Results m fO-Day Old Cotton WXmtm aai Fiaal TleM of S«ea Cottorn im lbs. Per Acre,
CSes«lt» IrnsM mm tmsh weight|
1 • F fetiol®
HO3N tl®M
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3 8 4 .©©55 i'»4t 4 4 4 .§14f 18©fi 4 8 S 18f4
4 8 4 ,mm S3?# 4 S 4 .90i§ lt§7 4 8 s zm$
5 8 4 .051© t42f 4 8 4 .Ql§2 BSf®' 4 8 4 .tfg SSfS
S 8 4 ,09 2f M06 4 li 4 •©tl8 mm 4 8 5 , •.•8i8 iS4f
^ 1000 Ifes. ©f- fsi-tilizer iaAl^atM afpllei p#?
® Mm&h figKi^ is an &w&Tmm &f repllo©ti®as.
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4?
earefMllr as t© avoid lta®Mag the att,t3?i«ts froa tb®
fdts.. nay kav# ©amasi ©©s,si4«3pa'bl« moistma?® stress
t# i«v«3.#f m& teomg.bt a¥omt a -reimttiQa ia growth,
laefe p©t la the f©mr^ iav«stigatl®a was proviitfi with m
«aa»@l«i pm t© mt&k tfe® leaeliat# %hm aakiag it possibi®
t# wat®r t&# plaats fb« l®a©tot# was rtturaei to
tfe® pots ferioditally, fh« tw@*gall©® pots tts®4 ia this
flias® ef tfee mwk wmm fillei 14 Hi wask«t mmk iaM#
Ww tisM# harvests weir® mat®, at 3§, ®0, 90, aad MS
Aays aft»r «»@ri®ii#®« As tfte plasts w®p« too »»al3. at SO
tays to frovlA® »mffle4«at f«ti©ie mterial, tlto tatir®
above gr©iia4 portiom was laiirv®st«t* F«ti©l@s froa tfe®
third aai fowtfe. aod®# fitm tfe® mim. st®a mftx w«r® tak®a
©a th« othtr fearvost iat®®, fii® a«fi®s war® iri»i aat
th® ®li«aieml t«t®r»iBBti©a« w®r« »at# ia tli® ®aii>® aiaaaer
as tfeoi® of the saooai iav«stigatioii.
Ilaight aad iry w®i#it measnroaeats also w@r@ wiie
daring th® oomra® of this ittv»8tigaties» fh®s® 4ata for
the four harvest tot®# ar® giv®a Im tafel# 11.
mirty <lay» aftar @«®rt«oe® th® '"low* tr«atm®Bt8 of
aitrogaa, pMspMrms^ aai potasaiia. w@r® aigaifioaatly
shorter thaa th® p'laats in th« **hi^« a®rl®»* M© r«al
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fatel© 11
Height aai Ory Weigbt as IsflmmmA hf Ntttrl©at •'fif®ata©ats itoiag %h.@ t&wtth lavestlgatlqa
iw-day ^ ^ fo^bfty l^^-&y
efi* BIT
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«». Bry Wt. i©i^t
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iitLr ¥ mmm 5ry It.
Imt mitm-gmm Met. « mm *
15.®* If.S 18.0
.390
.460
.532
51.5 89.6 64,4
5.11** f.61
10.to
103 122 108
132^ 131 llf
Si.i** 65.8 72.4
l w fJhosftoorms
•
14,0** 17.9 19.0
.355
.455
.532
56.7 C2.4 64.4
6.66» 7.92*»
lo.tt
129 115 108
144'^* 125 117
§6.1 65.0 72.4
%&W BV^tWt »a. » HigM. •
18,0 18..5 If.O
,455 .450 .53B
it.s §5.4 §4.4
6.7t** S.$6**
lO.tO
117 117 108
129 129 117
68.1 81.9* 72.4
I^w ealeim ««4. " ai^
If.S iO.I 10.0
.511
.sot ,53m
#8.1 6§.i #4.4
7.83»» 10,59 10.90
125 113 108
137** 122 117
81.0* 73.0 72.4
fO-'teSSilM' M@4. » ti# »
m,%** 18,4 19,0
,427 ,567 ,532
61,3 64.1 §4.4
8.34»* f.t4»»
10.90
109 122 108
115 131 117
62.4* 77.2 72.4
Ijm aaga«siwi Mei. " Hi^ *
1?.0 20. S 19.0
.5a« ,605 .532
§5.7 71.6 64.4
8,45** §.56*
10.90
124 112 108
lit 123 117
75.8 7S.4 72.4
* Siagl® asterisk, sigaifitant to .0§ l^vel. ** Boulile asterisk, sigaifteaat to .01 level.
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50.
fabl# IB
Itsuita 0f fissa# tssti oa 30-©af Harvest ©f lomirtli Iav©stlgsti©a»
Clesmlts tea®®a oa ©vea-tef wtlgMt)
fer«e ntm& ia tme^ 1 •W w en
I,©w »itrog«a Hit* " II,^^ •»
.138^ l.l8f 1.6i3
,874*»
.MM .3S3 .315
4. ©4 5.tl 4.il
3.4§ 3.71 3.4S
,3i3 *541 .53®
tm pb©sph6«s M#a. *• ligb **•
r •
1.580 I, am I. §03
,m 4.60 .249 3.78 .315 4.61 .858**
3.33 3.44 3.4®
.§95
.539
.536
Low stalfiap led, *» High "
I. §§8 1.3?® 1,603
.33®
.340
.315
4.71 4.§5 4.il
3.5® 3.48 3.46
.®45
.565 ,53i
I,®w' ealeiw l#t.. « High. « r =
lAm 1.411 i.isa
.268
.3E4
.315
4.44 4.St 4.®1
3. §5 3.E8 3,.46
.685
.i48 • .53i •.88S»*
p© tea Sim Met, » Itgfa
W m
i.iss 1.40g i.ess
• 547*
.321
.335
.315
2.77 3.84 4.Si
..88g»*
4.11 3.9S 3.4i -•771*»
.808
.712 • 53®
isw' tttgaositM M©a. " uigh «
1.181 i.iio 1.6^3
.319
.322
.315
4.48 4.47 4.61
3.57 3.49 3.4i
.520 • 573 .53®
%atir® abow fr©mai p^wtim of tto©,.'eottea plant was ^li.arT©fit®i. at -Kiis sampliag.
%a0li'fig«o?d is aa. avwag© ©f six Teplieatioas sigaificaat e©,i*r«latioa ©oeffieieats
^hmtwmm tt® eeaesatratios @f ia tk« p@tiol®8 ami amtr'itat tf-eatasat., .
•Sigaifie.aat %& ,05| •*algmifl0aat. to ..'01 la-rtl...
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51
U
ftsam# f#st ieamlts m ?©ti©l®8 ftom %Q»Mj Ilayvtst ©f •fenrtto latestigatioa.
Clesmlti fessti oa w«igM)
Peregat.agg ia Ga
Low- aitr©g«a .M«a, « Hi^ «
f «
.009^
.631 g.sio .8^»*
.185
.1^1
.IfS
f.45 7»5f $,Sf
.890**
^•§4 1.7f 1.71
.481
.367
.iOl
tm pfeospli®rm8 *«t. «' li^ «
y r
2.0B2 2.0S1 2.210
.14i
..13f 8.6g 8.13 §.#7
i..S4 8»ia 1.71 -.730**
.536
.464 •
.501
Jtow sulfiar
T «
a. 124 i.ifi g.gio
.ISf ,liS .IfS
8.0f S.f9 f.if
.68S* ,
g,«7 2.76 1.71
.488
.6g0
.§01
Low toloiiia M©a. « Hlgb «*
S.421 1.040 g.gio
..w am aw
8.13 7.98 9.67
i.Sg s,$s 1.71
.891
.@©4 .601
Low totassim *#4.• « 11# *• T t
I, §89 1.E33 a.810-
.158
.176
.173
4.17 6.04 9.67
,904**
3.16 1.018 3«©§ #648 1.71 .501 -.,801*».»87g*«
Lew mgii®aiwa Met. '" mm "
l.§4f g.m g.gio
.164
.180
.173
8.11 8.33 t.if
E..S9 g.88 1.71
.46§. ,§m .501
^ la®^ figw# Is m aterag# &f few 3f#pllsatl©s«,
•• ^ Imat©&t«s gl^ifisaiat ssyrtlatioa. e^^ffititiita •feetw#®® tJa© ©o®o®n:t-yatloa &t la tfe®
amteieat
•'SIgaifleant t© ,05j •* sigaifi@aat t© #01 l#t«l'i
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52.
fable 14
Tissue Test Besults on Petioles fmrn. SO-Day Harvest of Fourtli Investigation.
(Results based on oven-dry weight)
Treatment in petlolQS N P K Ca m
Low nitrogen Med. » High "
r s
Ix)w phosphorus Med. " High "
r »
Low sulfur Med. « High «
r s
Low oaloium Med. " Hi# »
r a
Low potassium Med. ^ High "
r *
Low mgnesiua Med. " High "
r ft
^ Each figur-e ia an average of five replications.
^ Indicates significant coirelatiGn ooeffioients between the concentration of elements in the petioles and nutrient treatajint#
* Significant to .05; •* significant to .01 level.
,447^ .356 6.10 2.31 .436 .§10 .2§8 4.94 1.57 .368
1.04B .S18 §.83 1.47 .454 .918** -.862** — -..817** —
1.002 .130 5,42 1.81 .362 .8S9 .175 5.41 1.63 .460
1.048 .E18 5.83 1.47 .454 — .703** •m m -.682**
.873 .B49 4.70 2.60 .431
.704 .gl7 4.86 1.52 .433 1.043 .218 5.83 1,47 .454
- - - - •.560* --
1.036 .245 5,55 1.29 .369 .721 .8E7 4.92 1.39 .400
1.042 .218 5.83 1.47 .454 - - - - .606*
,703 •211 2.29 2.58 .972 .700 .215 4.13 1.85 .624
1.04g .218 5.83 1.47 .454 -- — .954** -.918»*« ,760**
.911 .204 5.70 1.96 .286 • 758 .207 5.08 1.93 .380
1.042 .218 5.83 1.47 .454 — - - — .621*
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IteM aai lelativ#'faraitfttlaess of Ootton GomparM with' tissue test Result® frm 90»D«f F®ti@l@ Harvest of
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1, Oaroims, 1. L. fli« ma® of rapid ^©mleal plaat amtri«at testi im ftyMlizer .i©fi©i«i,®T iiaga&sti aat orop firgiaia trmel: Ixpt, Sta., ltil» ts, 1938 •
2. IstQB, I, 1, ls« of aitxie aoifi ia eontrol of pi aai aitrat# l@T#lfia amttiaat solmtiom.s* Plaat mjs,. lii itii.
§• latom,. t# M. lalatioas'of filj©y pfop@i?tl©8 of eottoa to aoistuff# gmpflf, frmltf«la#ss, aai eap'feolifdrat® aooimulatlos. 'fapmlllshai data. ProJtot lo* 4ga, t@3cas Agr. Ixp, ita« lf4f#
4, latoa, f, M, aai 1, 1» liglar. Iffeet of light iattosity, aitrogaa wtpply aaS fraltiag oa'ear^oMyaratt' atiliisatioa %f tli® oottoa plaat, flaat fliys# &0i 330-411. lt4S.
5, lEMyt, It 1. flaat tism® toats. as a gaii® to ftytilizar traatmaat of toiwto^s. EaataoSry A&t, ixf. Sta. Bal. 430, lf4g,
6, aayaaa, 1. I. iffaet of potaislum fartlliaatioa ©a tfci® mptaka of eatioas'tey tb@ oottoa plaat mHar ^otabomsa ®eafiitlea»«' Sotttk Cayoliaa .Agr, lacp. Sta, 5ttM. Mamal laporti 4©-'44. If46,
f. loailaaij i, 1,, ami B, I. Araoa# flia watox ©mltart aatMofl foff'gipowiag'plaats witlioat soil. Saliforaia Am* Sxp* 1W8,
8. Hoffar, 1, fastiag mta. stalks o:fea»i@ally to ait ia tatamiaiag tiair plaat food mmAs, ladiaMi A®p, latp, Sta..lal. gtS, If26.
f. Masoa, T, 0,# aat B. FMllis. Staiiea oa tli# ti-aaspopt of aitrogaaoma imfestaaces ia tM® eottoa plaat. VI Ooaosraiag at© rag® ia the Umrk. .itoaala of Botaay 4fi 31i-SSi, 1934.
10. Malated, S. W, A ohamioal atm4j of tai©fc-t®at took-aiqu®» for • potaasiiuft aad ami aim. i&we* Mar. Soc. Amm, mt Si3*S41. It4g.
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H, Morgaa, M. F* OMemieal soil ilagaosis %j ml'rersal a©ll ttatlag syst®®, Geaatetlemt igr. Ixp. Sta« ;iml. 450, 1941,
IS, ©Isen, X# G»t aad fi, P. Bledsoe, ffe# ©hiimieal eoMjptositloii ©f the ootton plaat aai tftie uptake of aatrieats at different stages ®f mrmm. i«@rgla-A®r»'l3Ep, Sta. Bui, 2as. Itig,
13, F«a©li, 1,:,'aad L, 'laglisM,' Sufid mieir@eli«iiical ioil t®@ts. S#U S©i, 5f I ISf-lfS* lt44.
14, Se«s«tlt, 0. D, Talm® aat lialtatl®a,s @f aettods'of iiagaogiag plaat atitrl@at ae@i«., 'Ittttr Oro©« with Plaat Food Ifl N@, §, ll-lfi, 1943.
15, f&€>aa»> ^W., aai'W, B. Kaok, foliar dlagaoslst fkyflolegltml baleuac© 1i@tw#@a tfe® bases lla®, •aagatsia,. lyaa potash., • Flamt Phys, 14 s --".flS, If St,
16, fboaas, f,, 1. S., la©k, aai 1, M, laha, foliar il.agaeals aai plaat awtrltlea f«rtllla®r pla««<®at ifEp®rla«at», i©wt* Mtr, So®, Agroa, 36t Sat^fOt, lt44.
17, f&oratoa# 8. f»,, S, -Coaaer, aai !• S, frasstr, • • m m ©f rapli ©fatmleal tests ©a aolli aat
flaats as alia la ittermlalag f®rtllla«r a®®ds, lailaaa A©f. laqp, Sta, Clr, i04, IfSf,
18, fya«r, 1, H, fb# rtlatloa of aora ,rl#ia» tO' l@af altroiea.:, p&ospliorma, aai' potaaslaii ©oateat,
aa®r, a©®. Soli Sel,. Ill Slf-SgS,. 194%,
It, 'SymTr Htf aad J, 1, rtlatloa ©f aora yl«Ms to autrlaaf tealaaa® a« rtT®al®d fer leaf' aaalrsla. Jour, Jaer.-Soa,, A®r©a, 38i. 173-188, 194$,•
B.Q, llrlolij, A.,., .flaat aaaljsla a« ,,a,.,ilaga©»tl0 pr©0#dur®» S®11,^ Sel, 5Sl 101-112, '"194'3,
®1, ?«al«, f, f, Ifutrltloaal laformatloa from plaat tlasu® tssti. Batter Crops wltk Plaat food lis 1©, §,'10-14, IMS,
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Wolf, B# lapii i®t@i?aiBati©a &t solmhl® 4ft soil aai flaat •trtrmets W mmm ©f a fto©t@ele«trle Imi# mi lag. 01i«a* 15s ^ a4®, 1®4S..
W©lfi 1#, aad ?• Idliliato... It^li ©j^taieal soil • aad flaat t«sts« S©il Sei. S4: gE7-S44« 1947,
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