tissue homogenate with precipitation tissue homogenate without precipitation cy5: pp2a 0.1 u cy5:...
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![Page 1: Tissue homogenate with precipitation Tissue homogenate without precipitation Cy5: PP2A 0.1 U Cy5: PP2A 1U Cy3: PP2A 0.1 U Cy3: PP2A 1U Cy5: PP2A 1U Supplementary](https://reader036.vdocuments.site/reader036/viewer/2022062500/5697c01b1a28abf838ccf9f8/html5/thumbnails/1.jpg)
Tissue homogenate with precipitation
Tissue homogenate without precipitation
Cy5: PP2A 0.1 U Cy5: PP2A 1U
Cy3: PP2A 0.1 U Cy3: PP2A 1U Cy3: PP2A 1U
Cy5: PP2A 1U
Supplementary Figure 2
pH 7pH 4
![Page 2: Tissue homogenate with precipitation Tissue homogenate without precipitation Cy5: PP2A 0.1 U Cy5: PP2A 1U Cy3: PP2A 0.1 U Cy3: PP2A 1U Cy5: PP2A 1U Supplementary](https://reader036.vdocuments.site/reader036/viewer/2022062500/5697c01b1a28abf838ccf9f8/html5/thumbnails/2.jpg)
Cy5: PP1 1U
Cy3: PP1 1U Cy3: PP1 10U
Cy5: PP1 10U Cy5: PP1 1U
Cy3: PP1 1U
Tissue homogenate with precipitation
Tissue homogenate without precipitation
Supplementary Figure 3
pH 7pH 4
![Page 3: Tissue homogenate with precipitation Tissue homogenate without precipitation Cy5: PP2A 0.1 U Cy5: PP2A 1U Cy3: PP2A 0.1 U Cy3: PP2A 1U Cy5: PP2A 1U Supplementary](https://reader036.vdocuments.site/reader036/viewer/2022062500/5697c01b1a28abf838ccf9f8/html5/thumbnails/3.jpg)
Cy5: AP 1U
Cy3: AP 1U Cy3: AP 10U
Cy5: AP 10U
Cy3: AP 10U
Cy5: AP 10U
Tissue homogenate with precipitation
Tissue homogenate without precipitation
Supplementary Figure 4
pH 7pH 4
![Page 4: Tissue homogenate with precipitation Tissue homogenate without precipitation Cy5: PP2A 0.1 U Cy5: PP2A 1U Cy3: PP2A 0.1 U Cy3: PP2A 1U Cy5: PP2A 1U Supplementary](https://reader036.vdocuments.site/reader036/viewer/2022062500/5697c01b1a28abf838ccf9f8/html5/thumbnails/4.jpg)
Cy5: -PP 10U
Cy3: -PP 10U Cy3: -PP 100U
Cy5: -PP 100U Cy5: -PP 100U
Cy3: -PP 100U
Tissue homogenate with precipitation
Tissue homogenate without precipitation
Supplementary Figure 5
pH 7pH 4
![Page 5: Tissue homogenate with precipitation Tissue homogenate without precipitation Cy5: PP2A 0.1 U Cy5: PP2A 1U Cy3: PP2A 0.1 U Cy3: PP2A 1U Cy5: PP2A 1U Supplementary](https://reader036.vdocuments.site/reader036/viewer/2022062500/5697c01b1a28abf838ccf9f8/html5/thumbnails/5.jpg)
Cy5: AP 10U , pH 9
Cy3: AP 10U , pH 9 Cy3: AP 10U, pH 9
Cy5: AP 10U, pH 9
Tissue homogenate with precipitation
Tissue homogenate without precipitation
Supplementary Figure 6
pH 7pH 4
![Page 6: Tissue homogenate with precipitation Tissue homogenate without precipitation Cy5: PP2A 0.1 U Cy5: PP2A 1U Cy3: PP2A 0.1 U Cy3: PP2A 1U Cy5: PP2A 1U Supplementary](https://reader036.vdocuments.site/reader036/viewer/2022062500/5697c01b1a28abf838ccf9f8/html5/thumbnails/6.jpg)
Mitochondrial-enriched fraction resuspended in SDS/HEPES buffer (H-buffer)
Mitochondrial-enriched fraction resuspended in phosphatase buffer (P-buffer)
Cy3: AP 10U Cy3: AP 10U
Cy5: -PP 100U Cy5: AP 10U
Cy3: -PP 100U
Cy5: AP 10U
Supplementary Figure 7
pH 7pH 4
![Page 7: Tissue homogenate with precipitation Tissue homogenate without precipitation Cy5: PP2A 0.1 U Cy5: PP2A 1U Cy3: PP2A 0.1 U Cy3: PP2A 1U Cy5: PP2A 1U Supplementary](https://reader036.vdocuments.site/reader036/viewer/2022062500/5697c01b1a28abf838ccf9f8/html5/thumbnails/7.jpg)
0.5U AP: Cy3 (green)
0.5U PP1: Cy5 (red)
0.5U PP2A: Cy2 (blue)
Supplementary Figure 8
pH 7pH 4