time- and frequency- resolved fluorescence made easy … · time- and frequency- resolved...
TRANSCRIPT
Key Benefits
Add GEMINI Interferometer in between the sample and the detector to easily retrieve the SPECTRUM of your signal.
• High throughput that allows high sensitivities
• User-friendly software
• Variable spectral resolution (without affecting throughput)
• Exceptional stability and insensitive to vibrations • Compact, lightweight and turn-key
• Broadband spectral coverage (UV to SWIR)
B
TIME- AND FREQUENCY- RESOLVED FLUORESCENCE MADE EASY
Measurement Example
1 2 3
Decay Time (ns)
10 1
10 2
Inte
nsity
(arb
.un.
)
Rhodamine B
Nile Red
A: Fluorescence Map of a sample (mixture of Rhodamine B + Nile Red in Ethanol) as a function of Emission Wavelength and Decay Time. B: Dynamics corresponding to vertical cuts in (A). C: Spectra corresponding to horizontal cuts in (A).
560 nmτ=2.85 ns
630 nmτ= 3.68 ns
1 nsA B C
6 ns
3 ns
FAST
DEC
AY
SL
OW
D
EC
AY
550 600 650 700
Emission Wavelength (nm)
0
50
100
150
Inte
nsity
(arb
.un.
)
500 1000 1500 2000 2500 3000 3500
Wavelength (nm)
0
10
20
30
40
50
Spec
tral R
esol
utio
n (n
m)
400 600 800 1000
Wavelength (nm)
0
1
2
3
4
L version
M version
S version
SimDOME project has received funding form the European Union’s Horizon 2020 Research and Innovation
Programme, under Grant Agreement n. 814492
• Interferometry• Generation of pulse pairs
GEMINI IN EXCITATION PATH• Fluorescence Excitation-Emission Map• Characterization of single molecules
GEMINI IN DETECTION PATH• Time- and frequency- resolved fluorescence• Pump-probe spectroscopy• Coherent Raman spectroscopy
Other Applications
Via Giovanni Durando 39 • 20158 Milan (Italy)www.nireos.com • [email protected]
Time- and frequency-resolved fluorescence with a single-pixel detector and a TCSPC module
Pulsedexcitation
Sample
SPAD(or PMT)GEMINI
Interferometer
Time Correlated Single Photon Counting
(TCSPC)
Experimental Setup for Time- andFrequency-resolved fluorescence
GEMINI provides the SPECTRAL resolutionTCSPC provides the TEMPORAL resolution
Spectral Resolution
Sensitivity Test
The GEMINI can be placed as a turn-key add-on device
10-12 10-10 10-8 10-6Concentration (M)
102
103
104
105
106
107
108
Cou
nts/
seco
nd
Background
DatapointsLinear Fit Measurements at different concentrations of the
sample have been performed (red dots).
The outstanding sensitivity of the system enables to reach picoMolar concentrations.
SNR=2
Excitation Power: 275 µW @ 532 nm wavelength
Sample: Mixture of Rhodamine B and Nile Red in Ethanol
Valid for asymmetric versions
Perri et al., Opt. Express 26, 2270-2279 (2018)