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Thermo Scientific DFSHigh Resolution GC/MS
Part of Thermo Fisher Scientific
m a s s s p e c t r o m e t r y
Designed for Highest Sensitivity
Food Safety
Environmental Analysis
Doping Control
Petrochemistry
Thermo Scientific D
FS — H
igh Resolution GC/M
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Thermo Scientific DFS — High Resolution GC/MS
The Thermo Scientific DFS is the most advancedhigh resolution mass spectrometer built for target compound analysis and for solving generalorganic analytical questions.
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The DFS delivers
• Highest sensitivity• Highest signal/noise• Lowest limits of detection• Lowest limits of quantitation• Highest selectivity • Highest sample throughput• Unattended automatic operation
Running the DFS is as simpleand straightforward as operating a low resolutionbenchtop MS.
• Automatic tuning• Automatic universal mass calibration• Intuitive user interface• Intuitive method and sequence set-up• Comprehensive and flexible data
evaluation and reporting
The DFS is economical …
• Fits into a small laboratory• Uses less power• Quieter• Requires little installation time• Immediately productive• Produces less heat
... and it is versatile
• Highest sensitivity EI/CI• Positive and negative ions• Solids probe• FAB... and other ionization and inlet techniques
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Experience the New Dimension
The revolutionary ion optics and the intuitive user interface make operation of the instrument easy and straightforward.
TechnologySeveral new technologies have been incorporated in into this revolu tionary massspectrometer to provide the most powerfulGC/MS mass spectrometer ever designed.
Novel AnalyzerThe newly designed analyzer is the firstwith virtually no image aberrations. It isperfectly double focusing, employing anultra high precision toroidal electrostaticanalyzer and a carefully refined magneticanalyzer. This directly translates into stability and ruggedness• Tuning for highest sensitivity does not
influence the preset resolution• Fully automatic ion source tuning• Automatic setting of mass resolution• No tuning of other ion optical elements• Mass calibration completely independent
from any scan or tuning parameter• Single universal mass calibration for all
modes of operationThe DFS is forgiving and robust while
delivering the highest sensitivity and selectivity.
Ion SourceThe ion source has been designed with special emphasis on sensi tivity, durabilityand ease of maintenance. Low maintenanceand short downtime increase productivity.• Exchange of ion volume and filament
without venting• Plug-in ion source for easy maintenance• Optimized long lifetime filaments for EI
and CI• New easy-to-use high stability reference
inlet system
ElectronicsThe DFS has completely new, state-of-the-art electronics. It uses dedicated micro-con-trollers on all boards, enabling efficient andfast computer control and read-back of allimportant parameters and voltages througha common inter connect bus. The magneticfield regulator is fast and stable, yet veryenergy efficient, utilizing ‘power-on-demand’ switching power supplies. It fully complies with the EU power factor correction directive. The magnetic field iscontrolled with 20 bit digital reso lution and receives its feedback from a uniquemagnetic field probe, supporting the singleuniversal mass calibration.
Chassis and CabinetThe new small-footprint cabinet is aestheticand functional. It contains the analyzer, theelectronics, and the complete pumping sys-tem, including roughing pumps. No preciousspace is wasted. Through its ingeniousdesign it allows the DFS to be shipped completely assembled with the magnet inplace. This means very short installationtime, no need for a crane, and the securitythat the system arrives exactly the way ithas been tested in the factory. Due to theinnovative technology the mass spectrome-ter weights less than conventional magneticsector instruments, thus minimizing thedemands on the laboratory environment.
Tune SoftwareThermo Scientific TunePlus is the renowneduser interface for ion trap and triple quadrupole MS (Thermo Scientific LCQ, LTQ, LXQ, TSQ Quantum). For the first time,a magnetic sector mass spectrometer usesthe same concept, making tuning, experi-ment and sequence set-up intuitive andsimple. High resolution MID has now beenadded as an integral part of the user interface.
GC/MS ProductivityThe DFS delivers unprecedented flexibilityand productivity for large sample series. Itcan be optionally equipped with twoThermo Scientific TRACE GC Ultra units.The two GC units are simul taneouslyinstalled at the same ion source for separation on two independent GC setups.
It is a system designed for unattendedoperation, in which the Thermo ScientificTriPlus autosampler offers highest samplecapacity and ultimate sampling flexibilitywhile serving one or two TRACE GC Ultraunits from shared sample trays.
GCs can be equipped with a widerange of injectors, from cold on-column andsplit/splitless injectors, to advanced temperature programmed injectors (PTV),and the latest developments in large volume injection techniques (LVI).
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Unprecedented Target Compound Performance
PesticidesThe routine confirmation and quantitation of pesticides onthe DFS high resolution GC/MS are demonstrated from a complex biological matrix sample extract measured in highresolution negative CI mode. More than 40 compounds fromthe target list, representing various pesticides and herbicidesfrom multiple chemical compound classes, are monitoredusing high resolution MID and are unambiguously identifiedusing a combination of the retention time and the accuratemass information. Excellent sensitivity and quantitative linearity over a wide concentration range down to the low femtogram level is shown in Figures 1 and 2 for Tolylfluanid,a phenylsulfamide fungicide. Figure 1 presents a measure-ment of ca. 25 fg, analyzed directly from a raw sampleextract. Very little matrix interference is observed. Figure 2shows the excellent lin earity of the method over the investigated concentration range.
Dioxins and FuransThe new DFS high resolution GC/MS is the reference instru-ment for the analysis on polybrominated and polychlorinateddioxins and furans (PCDD/F), delivering reliable results at thelowest detection levels ever achieved. Figure 3 presents atypical measurement of a food sample containing low concentrations of 2,3,7,8-substituted PCDD/F congeners. The chromato gram presented here on 2,3,7,8-tetrachlorodibenzo-p-dioxin corresponds to an approximate level of 20 fgof the substance injected on-column. Depending on thematrix interferences, detection levels in the single digit femtogram range can be achieved.
Figure 1: High resolution MID chromatogram for 25 fg Tolylfluanid injectedon-column in NCI mode.
Figure 2: Calibration curve for Tolylfluanid in NCI mode.
Figure 3: High resolution MID chromatogram of TCDD approx. 20 fg on-columnin a typical food sample.
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The DFS redefines performance
PerformanceThe DFS redefines performance. The inno-vative analyzer design provides the sensitiv-ity to routinely analyze compounds in thelow femtogram range.
Dioxin and furan analysis can be per-formed according to official internationalregulations such as EN 1948, EPA 1613, JIS K 0311/0312.
The unprecedented performance of theDFS will help you gain the largest amountof data from precious samples and lets youcut down on the amount of sample toextract. This is especially important in theultra trace analysis of lipophilic POPs. Thecleanup for these compounds is a lengthyand cost-intensive process employing sever-al chromatographic steps.
In cases where sensitivity is not suffi-cient, a higher amount of sample needs tobe cleaned up, which in certain cases canonly be accomplished by parallelization ofthe process. The sensitivity of DFS allowsthe lowest amount of sample use for quickcleanup. "Going Green" is realized on theDFS by a small footprint, less power con-sumption and minimum air condition load.
DFS Features• Mass Range of 2-6000 Da
• Scan speeds continuously variable from 0.1-10,000 sec/decade
• Radially laminated magnet
• Field based calibration, i.e. mass calibration is independent of scan speed, polarity, ionization mode
• High performance toroidal ESA
• Detection system with ± 20kV conversion dynode and long-life secondary electron multiplier for efficient detection of ions
• Full range of ionization modes available
• Pneumatically actuated exchange lock for ventless exchange of ionization volumes
• Water cooled DI probe and DCI probe available
• Ultra low detection limits for POPs
• Dual TRACE GC Ultra configuration with one TriPlus XT autosampler allows GC columns of different polarity to be used in one sequence. Samples can be injected from one vial into two different GCs in fully automated fashion
• Removable ion volumes for easy source maintenance
• Optional Dual Data Acquisition for increased productivity (For detailed information on this option see Thermo Scientific Application Note AN 30174)
• Going Green - best of its class for power comsumption and air condition load
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Thermo Scientific Xcalibur −A Unified Platform for MS
One intuitive platform for GC/MS, LC/MS, and Advanced MS instruments provides confident controlfrom method development to reporting.
Installation Requirements
Power 3-phase, 230/400V ± 10% 50/60 Hz, fused 25A per phase
Temperature 15-29°C (59-79°F) Air conditioning with better than 2°C stability recommended
Humidity 30-70%, non-condensing, non-corrosive
Cooling Water ca. 13 L/min, Temperature 20-25°C 3-5 bar (43-65 psi)
Helium For GC carrier gas: 99.996% or better, ultra-high purity. Total hydrocarbons should be less than 2.0 ppm
CI Gases Methane, isobutane, or ammonia; 99.8% or better
Compressed Air For operation of the pneumatic valves: 6-8 bar, filtered for particulate matter, oil mist and moisture
Pump Exhaust 25 mm, 1 L/min
Xcalibur PlatformXcalibur™ is the most powerful data systemavailable today, delivering a unique combi-nation of functionality, system control, andease of use. The software is designed toguide you through your daily analyticaltasks. This powerful simplicity combinedwith the advanced features of theMicrosoft® Windows® operating systemand Microsoft Office productivity tools provides an analytical platform that issecond to none.
The DFS generates high quality dataregardless of matrix and concentration, lowor high resolution, scanning or MID data.The enormous amount of information hidden in the data can be revealed usingthe built-in features of Xcalibur.
Xcalibur provides complete control ofthe DFS, the TRACE GC Ultra, and theTriPlus autosampler, as well as otherdevices. Xcalibur contains a built-in audittrail to ensure compliance with your labora-tory‘s SOPs and quality programs.
Generating reports from your data hasnever been easier than with XReport, thenew reporting application of Xcalibur.
Designed with ease of use in mind,XReport provides a flexible visual interfacefor intuitive report template creation andreport generation. Simple drop-down menusand ‘drag-and-drop’ objects provide com-plete flexibility for your reporting needs.
Integrates layered applications, including
• Mass Frontier™ – Simplifiesinterpretation of mass spectrawith improved predictive frag -mentation tools, and user friendlyclassification modules.
• Thermo Scientific EnviroLab Forms –Automated reporting forms packagedesigned for the regulated environ-mental laboratory.
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Thermo Scientific TargetQuan
The dedicated quantitation package is specifically designed for the isotope dilution technique, covering protocols like EPA 1613, JIS K 0311/0312, or EN 1948.
Dedicated Quantitation PackageTargetQuan, a dedicated quantitation pack-age specifically designed for isotope dilu-tion quantitation is available as part ofXcalibur. It has been tailored to the EPA,EN, and JIS protocols for analysis of per -sistent organic pollutants, such as halo-genated dioxins and furans, polychlorinatedbiphenyls, brominated flame retardants, andmany more. The software package consistsof three individual programs, TargetQuan,Response, and Reporter. TargetQuan is themain quantitation application, whichincludes the user interface for methodsetup, peak integration, and result viewing.Response deals with calibration curves,while Reporter helps to created printed orelectronic chromatogram documents.
Features
• Quantitation based on relativeresponse factors (RRF)
• User definable dependency of standards• One quantitation mass and up to two
ratio masses for confirmation based onisotopic abundance
• Freely configurable spreadsheet for dataviewing and method setup
• Automated or manual peak integration• Retention time correction
• Toxicity equivalents (TEQ) according toWHO definition including lower, medium,and upper boundary calculation
• User definable summation of calculatedamounts or TEQs for reporting of sumTEQ values
• Allows quantitation based on averageresponse of selected compounds (for EPA 1613)
Ready To Go
The software comes with templates forquantitation of polychlorinated dioxins,furans (like EPA 1613 and more), and other POPs.
AutomationBuilt-in scripting allows for full automationfrom creating a calibration curve to measur-ing samples unattended. The simple script-ing functionality enables you to tailor thesoftware to follow the workflow of yourlaboratory.
ReportingSpreadsheets can be printed, saved, export-ed, or emailed directly from the TargetQuanapplication. The look of these spreadsheetsare user definable, such that columns (rep-resenting values), rows (representing indi-vidual compounds), headings, and displaysettings can be arranged in any desiredarrangement, and can be saved as layoutfiles for easy access. The Reporter applica-tion comes with an intuitive editor for creat-ing reporting templates using ‘drag-and-drop’ objects.
Using the templates, chromatogramreports can be either printed or stored as document files auto matically from a sequence.
LIMS ExportAny selected set of parameters and com-pound can be exported to a LIMS systemusing CSV, ASCII, or XLS file export. Fileexport can be initiated automaticallythrough a sequence, or manually.
Single GC configuration
Dual GC configuration
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BR30068_E 03/10C
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