MINI THESIS

SCHOOLWIDE ENRICHMENT MODEL

MARA JUNIOR SCIENCE COLLEGE MUKAH, SARAWAK

2014

THE USES OF SNAIL AND SLUG SLIME

SCIENCE

GROUP: KANZEN CHOWA

RIYADH FAHMI BIN FAIZAL 144089

AMIRUZ ZAINULARIFFIN BIN ZULKURNAIN 144110

MOHAMMAD IMRAN BIN BOLHEE 144075

WRITER’S CONFESSION

We admit that this project report is the result of our own work except for excerpts and summaries of each of which had I explain the source.

Student : ………………..

(RIYADH FAHIMI BIN FAIZAL)

College No. : 144089

Date : ………………..

Student : ………………..

(AMIRUZ ZAINULARIFFIN BIN ZULKURNAIN)

College No. : 144110

Date : ………………..

Student : ………………..

(MOHAMMAD IMRAN BIN BOLHEE)

College No. : 144075

Date : ....................

2

FACILITATOR’S CONFESSION

I confirm that this mini thesis is the result of the students to meet their graduation requirements.

Facilitator : ………………..

(AHMAD SYUKRI BIN HARIRI)

Date : ………………..

3

APPRECIATION

Bismillahirahmanirrahim,

Thanks to God for giving us the chance to finish this mini thesis. This thesis which is entitled

“THE USES OF SNAIL AND SLUG SLIME” is one of the enquiries for us to graduate from

MARA Junior Science College. We are very grateful to every single person that had helped us in

finishing this thesis. Firstly, a massive thank you we bid to our principal, Mr. Abdul Aziz Bin

Baba for giving us the spirit on continuing our studies and the spirit to never give up.

Furthermore, we would like to say thank you to our dedicated teachers for helping us throughout

the whole process of the program. Moreover, our parents that had gave us the opportunity to

study in a higher level. Lastly, our friends that had lend us their hands whether in a big matter or

small matter.

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CONTENTS

PREFACE 1-6

INTRODUCTION 7-11

LITERATURE REVIEW 14-15

METHODOLOGY 16-17

RESULT 18-19

DISCUSSION 20-22

CONCLUSION 23-24

BIBLIOGRAPHY 25-26

ATTACHMENT 27

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ABSTRACT

THE USES OF SNAIL AND SLUG SLIME

MARA Junior Science College Mukah

Sarawak

This research is studied from the internet and experiment. Based on the data that we had collected, the slime produced by terrestrial molluscs (slugs and snails) has interesting properties

which have been utilized for centuries for the treatment of minor wounds and other skin disorders such as warts. This paper provides an introduction to the properties of slug slime and considers its potential value in modern wound management. It also reports the results of a small

study in which this material was successfully used to treat a longstanding wart.

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CHAPTER 1:INTRODUCTION

1.1 Introduction

1.2 Problem statement

1.3 Objective

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1.1 INTRODUCTION

Slugs and snails are terrestrial molluscs which have similar morphology except that slugs, unlike snails, have no obvious shell, although some species possess a partial or internal vestigial shell. Widely distributed around the world, the largest species of slug in the UK, the Ashy-Grey slug (Limax cinereoniger), can exceed 25 cm in length.

Both slugs and snails secrete visco-elastic slime or mucus which acts both as an adhesive and lubricant and enables the creatures to adhere to, and glide over, all types of surfaces including rough or potentially hostile terrain. Mucus also helps to prevent the creatures from drying out, renders them fairly unattractive as food for predators, and is also thought to help prevent infection and facilitate healing.

For centuries snails, and to a lesser extent slugs, have been used both as a food and as a treatment for a variety of medical conditions. In America slugs are not thought to be swallowed live in this way, but a recipe for ‘Slug Syrup’ is recorded on the website of the University of Saskatchewan. This instructs that a jar be filled with alternating layers of slugs and sugar. After about a day, when the sugar has ‘dissolved’ the slugs, the resulting mixture is run through a sieve, after which 1/3 grain alcohol is added by volume. The site quotes the original authors who recommend the resulting syrup be used for the treatment of ulcers, bronchitis, asthma, claiming that it is able to ‘heal these conditions when nothing else will.’

This observation resulted in the production of ‘Elicina’ a Chilean snail slime-based product. In 2010 ‘Missha’ then launched Super ‘Aqua Cell Renew Snail Cream’, claiming that its 70% snail extract ‘soothes regenerates and heals skin’. Snail slime based products are claimed to be the new miracle face-fixer in the U.S where they are used to treat acne, reduce pigmentation and scarring, and combat wrinkles.

In addition, in a special ritual slugs themselves are used for the treatment of warts. Mucus from a live slug is first rubbed onto the wart, and then the slug is hung out in the sunshine to dry out and die. It is believed that once the slug has dried up, the wart should as well. The use of slugs for the treatment of warts is not, however, confined to Italy. Records exist of the use of slug slime in the US and UK some of which recommended that the slime be collected at certain phases of the moon to ensure maximum effect.

Evidence of this practice may be found amongst the specimens contained in the Pitt Rivers Museum in Oxford where a glass specimen jar filled with alcohol contains a slug impaled on a thorn. Purchased by the Museum in July 1898 from Thomas James Carter of Oxford, it provides hard evidence of a cure used in several parts of the UK. The label on the jar instructs ‘Go out alone and find a large black slug. Secretly rub the underside on the warts and impale the slug on the thorn. As the slug dies the warts will go’. In other parts of the UK such as Berwickshire, Northumberland and Lancashire, the museum suggests that the slug is replaced by a snail: ‘Take a black snail, rub the warts with it, and then suspend it upon a thorn; as the snail melts away, so

will the warts. This must be done nine nights successively, at the end of which time the wart will completely disappear.

Snail products may even have a role in orthopaedics. The authors postulated that in the long term their observations could point the way to the development of bone cement based on a natural process involving inorganic crystals in an organic matrix; a biologically compatible material which might contribute to mechanisms of bone healing.

The principal benefits associated with the use of slugs and snails as topical treatments are therefore associated with the chemical and/or physical properties of the slime or mucus that they produce in abundance, particularly when threatened or irritated.

Production and properties of slug mucus relevant to wound healing

The mucus producing cells are located in the epithelium of the skin, both on the foot and upper surface of the body. Slugs produce at least two types of mucus; pedal mucus, which is relatively thin and contains about 96-97% water, and a second form which is produced over the entire body. This tends to be more thick and sticky. Both types are hygroscopic.

The precursor of slime is initially produced by the slug or snail in the form of highly hygroscopic grains which are stored within the cells in the form of granules coated with a protective water resistant membrane which keeps them dry. These packets only break open after they have been released from the cell, a process which is thought to be mediated by contact with extracellular ATP. At this point the granules very rapidly absorb up to 100 times their initial volume of water to form the familiar mucus or slime trail.

Slime is a complex material with non-Newtonian properties. In simple terms, the slime acts like a solid glue at rest, but liquefies when an adequate stress (or force) is applied to it - rather like non-drip paint or ketchup. When the applied stress is removed, the slime quickly re-solidifies. This may have important implications for its use as skin or wound treatment but slugs and snails use this property to create ‘pedal waves’ in a process known as adhesive locomotion. By exploiting this ‘yield-heal’ property, the creature can keep one part of its foot stuck to a surface whilst the remainder moves forward.

Composition of slug/snail slime

The composition of slime is thought to vary according to species, and it is believed that it is possible that each may also be able to vary its formulation.

Mucus consists of a complex mix of proteoglycans, glycosaminoglycans, glycoprotein enzymes, hyaluronic acid, copper peptides, antimicrobial peptides, and metal ions. Atomic absorption spectrometry showed that glue from the slug Arion subfuscus contains substantial quantities of zinc, iron, copper and manganese. Experimentally it was shown that the addition of iron or copper to dissolved slug glue causes the proteins to precipitate rapidly but the addition of zinc had no effect, suggesting that some metal ions play an important role in gel formation.

The presence within the slime of these complex polymers, may have particular relevance for wound healing as the literature contains many references to the importance of these materials in the healing cascade.

Studies have also shown that mucus contains peptides such as mucin which possess antibacterial activity against both Gram positive and Gram negative bacteria. These antimicrobial peptides not only act as natural antibiotics, but also stimulate many elements of the immune system, including barrier repair and inflammatory cell recruitment. The antibacterial factor from the body surface of the Giant East African Snail, Achatina fulica, for example, exhibited highly positive antibacterial activity both for the Gram-positive bacteria, Bacillus subtilis and Staphylococcus aureus and for the Gram-negative bacteria, Escherichia coli and Pseudomonas aeruginosa, but this activity was lost when the material was heated at 75º C for 5 min. The antibacterial factor of the snail mucus was shown to be a glycoprotein with a molecular weight of about 160,000.

Slug slime is also said to contain a local anesthetic and for this reason there are anecdotal accounts of live slugs being used to treat toothache.

These local anaesthetic properties (if confirmed) coupled with the antimicrobial properties and hygroscopic nature of the slime might offer significant benefits in the treatment of minor but painful wounds such as superficial burns in humans.

In the United States a patent has been filed (US2009026349) which describes the possible use of slug slime as a carrier for therapeutic agents in the treatment of burns and skin conditions and also as a protective covering for these and other wounds. Within the patent the inventor described how he had used slug slime on a painful skin rash and subsequently upon self inflicted burns deliberately produced with a soldering iron. The slime was said to form a protective layer which eliminated pain and stayed in place during showering

Scientific evidence which provides some credible basis for the possible use of slime in wound management may be found in report published in 2008. The authors found that slime from Cryptomphalus aspersa (also known as H. aspersa or the common garden snail) contains antioxidant superoxide dismutase (SOD) and Glutathione-S-Transferase Activity (GST) activities. Antioxidants are substances that may protect cells from the damage caused by unstable molecules known as free radicals or reactive oxygen species. SODs act as antioxidants and protect cellular components from being oxidized by reactive oxygen species. The authors also reported that the snail slime stimulated fibroblast proliferation, extracellular matrix assembly and the regulation of metalloproteinase activities and concluded that these effects together provided an array of molecular mechanisms underlying the secretion’s induced cellular regeneration, thereby supporting its possible use in repair of wounded tissues. In a subsequent study it was also demonstrated that the slime increased migration and increased the expression of cell-cell and cell-substrate adhesion molecules in mammalian fibroblast and keratinocyte cells.

It should be noted that some of these properties are analogous to claims made for some modern wound management materials.

Slug slime in the treatment of warts: an experimental study

In order to determine if the folklore surrounding the use of slugs for the treatment of warts had any apparent merit, slug slime was applied to a fairly large, longstanding filiform wart on the inner thigh that was rubbing on clothing and therefore scheduled for surgical removal. The wart was monitored and photographed over time resulting in what is believed to be the first fully documented and illustrated study of its type.

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1.2 PROBLEM STATEMENT

Nowadays, majority of people often say that snail and slug slime are useless substance. Actually snail and slug slime have a lot of benefits. We want to discover an alternative drug in

medical treatment. For in this case, snail and slug slime are use for the treatment of minor wounds and other skin disorders such as warts.

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1.3 OBJECTIVE

To overcome this problem, other alternatives should be used which is the snail and slug slime.

Based on the data that we had collected, the slime produced by terrestrial molluscs (slugs and

snails) has interesting properties which have been utilized for centuries for the treatment of

minor wounds and other skin disorders such as warts.

13

CHAPTER 2:

LITERATURE

REVIEW

14

Quave et al (scientist) described how, in southern Italy, the common garden slug, Arion

hortensis, is sometimes swallowed whole as a treatment for gastritis or stomach ulcers. In

the publication cited previously, Quave et al also described how slugs are used in Italy to

treat dermatological conditions. Mucus collected from a slug is rubbed onto the skin to

treat dermatitis, inflammations, calluses, and acne, and to promote wound healing.

Snail and slug slime have been used sporadically as skin treatments since the time of the

Ancient Greeks; Hippocrates reportedly recommended the use of crushed snails to

relieve inflamed skin and some 20 years ago, the potential of snail slime was noted by

Chilean snail farmers who found that skin lesions healed quickly, with no scars, when

they handled snails for the French food market.

Researchers at Herriot-Watt University found that the slime of Giant African land

snails contains unusual crystals of calcite. Under adverse conditions the snail will retract

into its shell and produce significant quantities of this slime which dries and quickly

hardens to form the animal’s epiphragm - a protective covering formed across the

opening of the shell when the snails go into periods of deep rest.

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CHAPTER 3:

METHODOLOGY

16

Slime was collected as required by irritating the dorsal surface of a specimen presumed to be

Arion ater, the European black slug, The slime was applied to the wart using a simple device

designed for the purpose consisting of a small plastic chamber cut from a strip of plastic

previously used to supply tablets. The apex of the chamber was removed and the plastic cup

attached to a piece of semipermeable polyurethane film to form an island dressing (Figure 2).

The slug slime was placed in the chamber and the dressing applied to the skin. Four applications

of gel were made over a period of about 8 weeks in the same way, with the exception that the

plastic chamber was not deroofed for the final two.

The principal reason that treatment was conducted over an extended period was to

accommodate a family holiday. Short intervals were also left between applications to monitor

any changes that might have occurred in the appearance of the wart and surrounding skin.

Under normal circumstances, however, it would be preferable to provide frequent and

uninterrupted applications of slime to facilitate or accelerate any possible therapeutic effect.

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CHAPTER 4:

RESULTS

18

During the course of the treatment, the moist conditions produced by the mucus initially caused

the wart to become hydrated which resulted in an apparent increase in size of the finger-like

projections growing from the surface. As the treatment progressed, however, these projections

were lost and eventually the location of the wart became virtually indistinguishable from the

surrounding skin.

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CHAPTER 5:

DISCUSSION

20

The results of this simple investigation provide convincing evidence that the use of the slug slime

coincided with major changes in the appearance of the lesion in question, although more comprehensive

controlled studies would obviously be required to make a more definitive statement on the efficacy of the

material for this indication.

The mechanism for the observed response of the wart to the slime treatment is unclear. It might be that

there is a physical effect, simply caused by changes in the hydration of the stratum corneum produced by

the semi-occlusive nature of the dressing. If this is the case it is postulated that conventional hydrogel or

hydrocolloid dressings should prove to be equally efficacious, and this might in part explain the reported

success of other folklore treatments involving banana skin or potato peelings which might produce a

similar effect.

A more interesting and exciting possibility, however, is that the slime is exerting some biological effect.

Warts are growths resulting from an infection caused by human papilloma virus and it is interesting to

speculate whether the slime has specific antiviral activity.

Although references have been identified that refer to antiviral activity of mucins found in the mucous

linings of the stomachs of pigs, no references have been identified which specifically attribute this

property specifically to mucus produced by terrestrial molluscs.

It would not be surprising if such properties did exist however, for slugs (and to a lesser extent snails)

have a moist skin and live an in environment heavily contaminated with potential pathogenic agents, and

thus might be considered to be at particular risk of developing infections. Mucus, like that present in the

nasal passages of mammals for example, functions as a selectively permeable replaceable physical barrier

between the host and the outside world. If slug mucus possesses antiviral properties in addition to its

antibacterial effects, its effectiveness in this regard will presumably be greatly enhanced.

If such activity is present, it could provide an explanation for the apparent ability of slugs to help remove

warts and perhaps potentially even treat other viral skin lesions such as those caused by Herpes simplex.

Irrespective of any possible antiviral activity, slug mucus appears to offer some real potential benefits in

wound management. The results of the biochemical studies cited previously provide scientific support for

a possible beneficial effect on the proliferation of fibroblasts and keratinocytes which could contribute

directly to enhanced healing. The physical properties of the mucus may also be important in that it

adheres well to human skin even in the presence of water as any gardener will confirm, and in this regard

may well out-perform most existing hydrogel products. Together all these observations suggest a number

of intriguing possibilities for the production of new dressing formulations that could represent a

promising area for future research.

The use of slugs and snail is not without risk, however, as these can sometimes act as vectors of disease.

E.coli and other bacteria present in their faeces have a relatively long external and internal survival time.

Slugs and snails can also become a vector of rat lungworm a disease caused by a parasitic worm

Angiostrongylus cantonensis. Normally carried by rats, the molluscs become infected by consuming the

infected faeces of carrier rats. The parasite develops further in the slugs and snails and if the infected

molluscs are consumed in turn by rats the life cycle is completed.

A. cantonensis generally poses relatively little threat to humans as infections are very rare, although they

can occur from consumption of undercooked or raw infected slugs and snails either by design or by

consuming produce that has not been adequately washed and therefore contains a small slug or a snail.

The fresh slime of snails and slugs can also have lungworms, which may be passed on to humans and

other animals, although the risks are probably lower with dry slime as outside of hosts the lungworm dies

quickly.

Lungworms are dangerous because once ingested they first head to the brain where they can cause

meningitis type symptoms, with damage to brain tissue and swelling of the brain before the lungworm

dies. Many people show no symptoms at all before the lungworm dies but others are greatly affected. In

Sydney in 2011 one baby girl died due to lungworm infection and adults have had severe brain injuries

after eating slugs. This small number of cases suggests that the risk of infection is possibly low, although

the consequences can be disastrous.

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CHAPTER 6:

CONCLUSION23

The results of the brief literature review identify some intriguing possibilities concerning the

potential value of slug secretions in the treatment of wounds. These findings, together with the

results of the simple study involving the treatment of a wart, provide some support for empirical

observations made in different parts of the world which led to the use of these materials for

medical purposes. Whilst it is true that in some instances these treatments also involve a degree

of magic or superstitious ritual, this does not of itself mean that they have no scientific merit.

Indeed, a direct parallel exists to the use of maggots, leeches, bees and bee products, all of which

were used for centuries before the actual mechanisms of action were clearly defined or

understood.

Whilst the plant kingdom has long been recognized as a key source of medical products, rather

less attention has been focused upon members of the animal kingdom in this regard.

Perhaps in the future it could be the turn of the humble slug to slither quietly into the medical

spotlight by providing a new treatment for wounds which incorporates agents that accelerate

healing whilst providing a degree of antisepsis and local analgesia.

24

BIBLIOGRAPHY1.Anon. Slug. Wikipedia 2013; Available from URL: http://en.wikipedia.org/wiki/Slug.html

2. Quave C L, Pieroni A, Bennett B C. Dermatological remedies in the traditional pharmacopoeia

of Vulture-Alto Brandano, inland southern Italy. Ethnobiol Ethnomed 2008; 4.

3. Cremati J. Sensitized Slug Slime Recipe. University of Saskatchewan 2007; Available from

URL: http://www.usask.ca/lists/alt-photo-process-l/200712/msg00196.html.

4. Reporter D.M. Snail slime hailed latest beauty wonder product, promising to 'clear acne,

reduce scarring and beat wrinkles'. Daily Mail 2012; Available from URL:

http://www.dailymail.co.uk/femail/article-2216457/Snail-slime-hailed-latest-beauty-wonder-

product-promising-clear-acne-reduce-scarring-beat-wrinkles.html.

5. Anon. Wart Remedies. www.thenakedscientists.com 2004; Available from URL:

http://www.thenakedscientists.com/forum/index.php?topic=1371.0

6. Richardson H. Slug on a thorn. Pitt Rivers Museum Website 2013; Available from URL:

http://england.prm.ox.ac.uk/englishness-slug-on-a-thorn.html.

7. Aitken R. Snail slime 'could mend bones'. BBC News Available from URL:

http://news.bbc.co.uk/1/hi/health/900869.stm.

8. Anon. . Wikipedia 2013; Available from URL:

http://en.wikipedia.org/wiki/Angiostrongylus_cantonensis.

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ATTACHMENT

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