“The thin wall ESI tip appears unusually

advantageous for ensuring stable ESI

conditions and efficient ion transfers.”

Valaskovic, Kelleher, McLaffertyScience (1996), 273, 1199

perfect zero-dead-volumePicoClear™ technology revolutionizes the way capillary connections are made. High performance applications that require connections with inline filters, liquid-junction voltage, sample-trap, or tees and crosses are now simple and easy! The PicoClear line of connectors can do it all–even at high pressure.

How does Picoclear™ technology work?PicoClear™ connectors feature pure radial compression to provide a high performance connection. This novel format ensures the perfect alignment and union of fused silica tubing – every time. Uniformly applied pressure surrounds the tubing, thereby eliminating friction and cracking that can lead to unwanted contaminants and result in a clogged nanospray emitter.

1 2 3Slide the first piece of tubing into the union, positioning it halfway through the window

Insert the second piece of tubing into the opposite side of the union. Gaps are clearly visible if present.

Tighten the end fittings to seal and compress the union. Full compression results in higher optical clarity.

Pure radial compressionThe radial compression provided by PicoClear connectors is a revolutionary development in LC-MS and eliminates frusto-conical compression connections featured in traditional fittings. Troubleshooting becomes diagnostic and clogging is virtually eliminated.

Elastomer coreThe elastomer core provides the platform for PicoClear technology and allows for the easy visualization of the perfect connection. Compression of the elastomer core results in a clear view of the union, and of course, a perfect connection.

Longitudinal compression shellapplies pure axial compression

Body

Fluoropolymer Core

1º Nut2º Nut2º Nut

1º Nut

Optically Clear Insert

Clearly, a revolutionary solutionBecause PicoClear connectors are clear, perfect contact is confirmed with a simple visual inspection. A clear solution to a difficult problem.

Body

Fluoropolymer Core

1º Nut2º Nut2º Nut

1º Nut

Optically Clear Insert

P icoclear™ connec tor s

PicocleartM applications

2d SeParationS & BeYond

Build your own, custom multi-phasic columns. Take advantage of PicoClear technology and the novel zero-dead-volume design to connect columns of differing or similar chemistries - with no loss in performance. It’s easy!

Zero-dead-volUMe liQUid JUnction voltaGe

Need high voltage capabilities? The PicoClear conductive union provides the same zero-dead-volume connection with liquid junction high-voltage capability. The application of high voltage is more stable and efficient.

Expanded chromatographic regions of BSA tryptic digest chromatographic peaks. Injection: 100 fmol BSA, Flow rate: 300 nL/min., Gradient: 2% - 50% B over 70 min. All connections made using PicoClear union.

C) PicoClear conductive union

A) Intact 30 cm C18-packed PicoFrit column

B) A C18-packed 20 cm IntegraFrit coupled to a C18-packed 10 cm PicoFrit column

An injection of BSA (300 fmol) was analyzed using three different liquid-junction high-voltage connection devices. The same column and emitter were used for all data collection.

A) Standard metal union

B) PEEK microtee with a platinum wire

viSiBle inline filter

Integrate PicoClear technology with an IntegraFrit™ nanobore column to create an in-line, metal free filter— A powerful combination.

Unpacked IntegraFrit column (on left) coupled with fused silica tubing (right) using PicoClear union

Carbon and other harmful particulates are clearly visible (after 2 hours)

Frittedtubing To column

Flow

Inside the PicoClear conductive union, highly efficient voltage contact is made within the zero-dead-volume liquid-junction connection

57.52

96.52

13.8245.22

73.22

95.91 74.82

15.8282.03

73.91

58.4333.9139.6 61.11 11.9133.41

:LN7E08.7

5 01 51 02 52 03 53)nim( emiT

Metal union

A

20 25 30 35 40 45 50Time (min)

NL:4.78E7

NL:5.61E7

NL:4.03E7

20 25 30 35 40 45 50Time (min)

20 25 30 35 40 45 50Time (min)

B

C

A

6.98E62.97E72.40E7

NL:7.11E6NL:2.58E7NL:2.20E7

NL:6.98E6NL:2.97E7NL:2.40E7

8.4 s 9.6 s 12.6 s

10.8 s 10.2 s 13.8 s

10.8 s 12.0 s 13.2 s

A

PEEK microtee

5 01 51 02 52 03 53)nim( emiT

80.82

51.82

27.52

25.91 12.8264.22

79.92

21.773.9162.8 51.9 54.21

80.03

:LN8E50.1

B

20 25 30 35 40 45 50Time (min)

NL:4.78E7

NL:5.61E7

NL:4.03E7

20 25 30 35 40 45 50Time (min)

20 25 30 35 40 45 50Time (min)

B

C

A

6.98E62.97E72.40E7

NL:7.11E6NL:2.58E7NL:2.20E7

NL:6.98E6NL:2.97E7NL:2.40E7

8.4 s 9.6 s 12.6 s

10.8 s 10.2 s 13.8 s

10.8 s 12.0 s 13.2 s

B

PicoClear Conductive Union

5 01 51 02 52 03 53)nim( emiT

51.62

65.0235.82

59.4298.22

95.82

52.0354.02

70.3363.0246.7 70.9 22.11 95.81

:LN8E40.4

C

20 25 30 35 40 45 50Time (min)

NL:4.78E7

NL:5.61E7

NL:4.03E7

20 25 30 35 40 45 50Time (min)

20 25 30 35 40 45 50Time (min)

B

C

A

6.98E62.97E72.40E7

NL:7.11E6NL:2.58E7NL:2.20E7

NL:6.98E6NL:2.97E7NL:2.40E7

8.4 s 9.6 s 12.6 s

10.8 s 10.2 s 13.8 s

10.8 s 12.0 s 13.2 s

C

PicoClearTM technology is an essential part of the high-performance analytical laboratory. Capillary connections are now robust and simple.

C) Two C18-packed 10 cm IntegraFrit columns coupled to a 10 cm PicoFrit column

Picoclear™ Union PCU-360PCU-150

Reusable bodySet of reusable nuts / ferrules

Replaceable clear inserts (2)Swaging tool

Picoclear™ condUctive UnionPCCU-360

Reusable conductive bodySet of reusable nuts / ferrules

Replaceable clear conductive insert (1)Bore rejuvenation kit

SPecificationS For 360 µm OD and 150 µm OD tubing

Length: 48mm - 50 mm Assembled (1.89” - 1.97”)

Height: 8mm (0.31“) Width: 9mm (0.35“)

Max. Pressure: 9,000 psi

SPecificationS For 360 µm OD tubing

Length: 48mm - 50 mm Assembled (1.89” - 1.97”)

Height: 8mm (0.31“) Width: 9mm (0.35“)

Max. Pressure: 5,000 psi

High-Performance. cost-efficient.Modern applications require the highest possible performance - sensitivity, selectivity, ruggedness. Use PicoClearTM connectors over and over again to ensure a perfect connection that is free of contaminants. A quick visual inspection of the PicoClear see-through elastomer core can confirm that your fluid path is in tip-top shape. When it is time to replace the PicoClear core, simply remove the used core and replace it with a new one.

Learn more about PicoClear technology on our website: www.newobjective.com

® 2009 New Objective, Inc. All rights reserved. PicoClear U.S. and Foreign Patents Pending. PicoView, Digital PicoView, PicoFrit, IntegraFrit, PicoTip, GlassTip, EconoTip, QuartzTip, PicoClear, and ProteoPep are trademarks or registered trademarks of New Objective, Inc. All other trademarks are the property of their respective companies.

New Objective, Inc.Two Constitution WayWoburn, MA 01810-1023

888 220 2998 Orders781 933 9560 Phone781 933 9564 Fax

sales@ newobjective.comwww.newobjective.com

New Objective, Inc.Two Constitution WayWoburn, MA 01810-1023

888 220 2998 Orders781 933 9560 Phone781 933 9564 Fax

sales@ newobjective.comwww.newobjective.com