the practical issue 18

Jeju Exhibition & Convention CenterJeju, Korea

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Jeju Fish-Culture Fisheries Cooperatives

ASSOCIATE SPONSORSAquaculture Engineering Society

International Association of Aquaculture Economics &Management

May 26-30, 2015

WORLDAQUACULTURE 2015Aquaculture For Healthy People, Planet and Profit

THE ANNUAL INTERNATIONAL CONFERENCE & EXPOSITION OFWORLD AQUACULTURE SOCIETY

ASIAN-PACIFIC CHAPTER, WAS KOREAN CHAPTER, WAS

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NEXT GENERATION GENOMIC STUDIES

The most threatening new problem in Asian shrimp aquaculture since 2009 has been serious mortality within approximately the first 35 days after stocking shrimp ponds with post larvae. The problem is commonly called early mortality syndrome (EMS) and started at the early of 2012 in Thailand and has caused Thai shrimp production to reduce 54% in 2013. The cause of EMS was originally unknown. We carried out a metagenomic analysis to determine whether any unique bacteria (culturable or unculturable) might be present in EMS ponds when compared to normal ponds. DNA was extracted from pooled HP tissue samples from normal and EMS shrimp ponds and subjected to PCR amplification of small subunit ribosomal RNA (ssu rRNA) gene fragments using universal bacterial primers. After primer and multiplex adapter sequence trimming, the remaining 59.7 million bases from 163,102 reads with lengths of at least 100 bp were used to construct unique sequences (operational taxonomic units, OTUs). Since no OTU were found to be exclusive to EMS ponds, we compared the proportion of reads for OTU between EMS ponds and normal ponds. This comparison revealed 32 OTUs of six genera from four algorithms that had a significantly higher proportion of reads in EMS ponds than in normal ponds. This comparison revealed OTU with high identity to expected Vibrio species (e.g., V. parahaemolyticus, V. harveyi and V. campbellii) and with high identity to other bacteria known from aquatic environments, but never previously reported as shrimp pathogens. The latter included the genera Delftia and Ralstonia from the order Berkholderiales and Leifsonia and Rhodococcus from the Order Actinomycetales. Preliminary studies with bioassay confirmed that these bacteria could be direct shrimp pathogens. Besides the bacterial

1 Shrimp-virus Interaction Laboratory (ASVI), National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Bangkok 10400, Thailand 2 Biostatistics and Informatics Laboratory, Genome Institute National Center for GeneticEngineering and Biotechnology, Pathum Thani 12120, Thailand 3 Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400 Thailand 4 National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani 12120, ThailandE-mail: [email protected]

pathogens, we also found the concurrent problems in shrimp associated with EMS including the occurrence of aggregated transformed microvilli (ATM). Preliminary study using the next generation sequencing and bioinformatic analysis suggested that there might be the pathogen

involved. Further investigations on these pathogens are urgently required in order to design the appropriate strategy to control EMS in shrimp industry.

ACKNOWLEDGEMENTS: We thank National Research

Council of Thailand (NRCT), National Center for

Genetic Engineering and Biotechnology (BIOTEC) and Agricultural Research

Development Agency (ARDA) for grant support for the study

on shrimp EMS.

Presented in "Next Generation Sequencing for Genetic and Genomic Studies (NGS2014)" organized by Mahidol University, 29-30 July, 2014 Winser Hotel, Bangkok, Thailand

Kallaya Sritunyalucksana1,Anuphap Prachumwat1,Sissades Tongsima2,Siripong Thitamadee3,Timothy W. Flegel4

Next generation sequencing uncovers unexpected pathogens associated with early

mortality syndrome (EMS) in shrimp

11

Case 11-041. Normal hepatopancreas (HP)

Uneaten Feed

No2 Gas

Bacteria

Bacteria

No2 Nitrite

No2 Nitrite

No3 Nitrate

Fixation

Mineralization

Algae bloom

Volatilized


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08:00 - 10:0010:00 - 11:0011:00 - 11:4011:40 - 12:0012:00 - 13:00

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16:40 - 17:0017:00 - 18:40

10:00 - 20:00

Registration & Inscriptions + Posters Assembly.OFFICIAL INAUGURATION BY FEDERAL AND LOCAL AUTHORITIES.MASTER CONFERENCE: "Strategies for the Development of Aquaculture in Mexico", TBA. Recess & Coffee break, Tradeshow.

TRADESHOW SCHEDULE.

Tilapia Aquaculture. - Nutrition.

- Moderator: Wilfrido Contreras, UJAT, Mexico; Kevin Fitzsimmons, U. of Arizona, USA.

Feeds & Additives.

- Moderator: Teresa Viana, UABC, Mexico.

Marine Finfish Aquaculture. - Reproduction & Genetics.- Moderator: Lorenzo Juarez, ANCPN, Mexico; Luis Andre Sampaio, UFRC, Brazil; Juan Pablo Lazo, CICESE, Mexico.

Production Systems. - Biofloc.

- Moderator; Wilson Wasielesky, UFRC, Brazil; Sergio Zimmermann, Zimmermann AquaSolutions, Brazil.

Production Systems. - Biofloc.

Production Systems. - Recirculation. - Moderator: Marcelo Shei, PREAMAR, Brazil; Michael Schwarz, Virginia Tech, USA.

Fish Morphology & Physiology.

- Moderator: Roberto Mendoza, UANL, Mexico.

Fish Morphology & Physiology.

Gender Inclusion in Aquaculture.

- Moderator: Felipe Matias, FAO, Brazil.

Feeds & Additives. Marine Finfish Aquaculture. - Larviculture.

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Crustaceans Aquaculture.- Health.

- Moderator: Scott Horton, Nutrimar, Mexico.

Lunch.

Recess & Coffee break, Tradeshow.

Friday, November 7th

09:00 - 11:00

11:00 - 11:2011:20 - 13:00

13:00 - 15:0015:00 - 17:40

17:40 - 19:0010.00 - 20:00

CLOSING EVENT, TRADESHOW.TRADESHOW SCHEDULE.

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- Moderator: Juan Pablo Lazo, CICESE, Mexico.

Fish Health.

- Moderator: Camilo Pohlenz,BIOMAR, Costa Rica.

Catfish, Carp & other Freshwater Species.

- Moderator: Francisco Arregui, S.P.-Bagre, Mexico.

Production Systems. - Recirculation. - Moderator: Marcelo Shei, PREAMAR, Brazil; Michael Schwarz, Virginia Tech, USA.

Ornamental Species. - Moderator: Marcelo Shei, PREAMAR, Brazil; Nuno Simões, UNAM, Mexico; Serafin Almenara, AZOO Mexico, Mexico.

Ornamental Species.

Reproduction & Genetics.

- Moderator: Ricardo Ribeiro, UEM, Brazil; Roberto Solis, Acuaplan, Mexico.

Reproduction & Genetics.

Career Seminary for Students.- Moderator: Juan Pablo Lazo, CICESE, Mexico; Ma. Celia Portella, CAUNESP, Brazil.

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Salmon & Trout Aquaculture.

- Moderator: Luis Hector Hernandez, UNAM, Mexico.

Fish Health.

Fish Feeding & Nutrition.

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- Moderator: Scott Horton, Nutrimar, Mexico.

Crustaceans Aquaculture.- Nutrition.

Crustaceans Aquaculture.- Nutrition.

Recess & Coffee Break, Tradeshow.

Lunch.

LYNX HALL AQUARIUM HALL ANTARES HALL PERSEUS HALL SCORPIO HALL ARIES HALL

Wednesday, November 5th

07:00 - 09:0008:00 - 16:0009:00 - 09:4009:40 - 10:2010:20 - 13:00

13:00 - 15:0015:00 - 17:00

17:00 - 17:2017:20 - 19:00

19:30 - 22:0017:00 - 20:00

Registration & Inscriptions + Posters Assembly.Tradeshow Assembly.MASTER CONFERENCE: "Effects of Climate Change in Aquaculture", Adalberto Val - Director of the National Institute for Amazonian Research, INPA, Brazil.Recess & Coffee break, Lobby.

Welcome Cocktail.TRADESHOW SCHEDULE.

Aquaculture of Indigenous Species.

- Moderator: Carlos Martinez, UMSNH, Mexico.

Mollusks Aquaculture. -Reproduction.- Genetics.

- Moderator: Sergio Guevara, CESAIBC, Mexico.

General Contributions to Aquaculture.

- Moderator: Helcio Marques, APTA, Brazil.

Ecology & Limnology.

Aquaculture Economics.

- Moderator: Fco. Javier Martinez Cordero, CIAD, Mexico.

Aquaculture Economics.

Crustaceans Aquaculture. - Macrobrachium and other crustaceans.- Moderator: Patricia Valenti, CAUNESP, Brazil; Eduardo Ballester,UFRG, Brazil; Fernando Villasante, UdeG, Mexico.

Education, Training & Technology Transfer. - Moderator: Newton Rodrigues, Brazil.

Aquaculture in LA&C.- Moderator: Wilfrido Contreras, UJAT, Mexico; Antonio Garza, LACC-WAS. Mexico; Felipe Matias, FAO, Brazil.

Mollusks Aquaculture. - Nutrition. - Grow-out.

Micro-Algae.

- Moderator: Pilar Sanchez, CICESE, Mexico.

Macro-Algae.

- Moderator: Pilar Sanchez, CICESE, Mexico.

Mollusks Aquaculture. - Environmental & Health Managment.

Aquaculture of Indigenous Species.

Fish Larviculture.

- Moderator: Ma. Celia Portella, CAUNESP, Brazil.

Sustainable Aquaculture. - For a changing World. - Financial Integration of sustainable models.

- Moderator: Adalberto Val, INPA, Brazil.

Sustainable Aquaculture. -Multi-trophic integrated aquaculture. - Moderator: Luiz Poersch, Brazil.

Sustainable Aquaculture. - Aquaponics.- Organic Aquaculture. - Moderator: Carlos Leon, BoFish, Mexico; Manuel Segovia, CICESE, Mexico.

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A regional professional network in aquaculture established in 2009, the AAN’s mission is to help aquaculturists and farmers operate a profitable and environmentally sound business, and in doing so, provide the world affordable products at high quality in a sustainable manner.

WORLD AQUACULTURE 2015 WILL FOLLOW THE VERY SUCCESSFUL FIRST KOREAN MEETING - WORLD AQUACULTURE 2008 IN BUSAN

World Aquaculture 2015 will be held in Jeju Island – one of the leading flatfish production centers in the world. The rest of the growing aquaculture industry in Korea and the Asian Pacific region will also be represented.

A major international trade show at WORLD AQUACULTURE 2015 is the place to learn about the latest in aquaculture, see the newest technology presented by exhibitors from around the world.

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the practical issue 18

Documents

ems ponds

ems shrimp ponds

normal ponds

stocking shrimp ponds

asian shrimp aquaculture

cause of ems

shrimp pathogens

orgblue aqua international