the plasma membrane · 2018. 9. 1. · 4 functions of plasma membrane provide a binding site for...
TRANSCRIPT
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The Plasma Membrane -
Gateway to the Cell
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Photograph of a Cell Membrane
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Functions of Plasma Membrane
Protective barrier (flexible)
Regulate transport in & out of cell (selectively permeable) Homeostasis
Allow cell recognition
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Functions of Plasma Membrane
Provide a binding site for enzymes
Provide anchoring sites for cytoskeleton
Contains the cytoplasm (fluid in cell)
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Structure of the Cell Membrane
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Phospholipids
Cholesterol
Proteins(peripheral and integral)Carbohydrates (glucose)
Membrane Components
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PhospholipidsMake up the cell membraneContains 2 fatty acid chains that are nonpolar (non magnetic)
Head is polar (magnetic) & contains a –PO4
(Phosphate)
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FLUID- because individual phospholipids and proteins can move side-to-side within the layer, like it’s a liquid.
MOSAIC- because of the pattern produced by the scattered protein molecules when the membrane is viewed from above.
FLUID MOSAIC MODEL
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Polar heads are hydrophilic “water loving”
Nonpolar tails are hydrophobic “water fearing”
Cell Membrane
Makes membrane “Selective” in what crosses
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Small molecules move through easily.e.g. O2, CO2, H2O
Semipermeable Membrane
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Large molecules such as proteins do not movethrough the membrane on their own.
Semipermeable Membrane
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Types of Transport Across Cell Membranes
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Simple Diffusion
• Requires NOenergy
• Molecules move from area of HIGH to LOWconcentration
Solvent/Solute/Solution
• Solvent is a substance that dissolves a solute to make a solution
• Water is the substance that dissolves sugar to make Sugar-Water
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Diffusion of Liquids
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Diffusion through a Membrane
Cell membrane
Solute moves DOWN concentration gradient (HIGH to LOW)
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Osmosis
• Diffusion of wateracross a membrane
• Moves from HIGH water potential(low solute What the water is in) toLOW water potential (high solute)
Diffusion across a membrane
Semipermeable membrane
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Diffusion of H2O Across A Membrane
High H2O potentialLow solute concentration
Low H2O potentialHigh solute concentration
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Cell in Isotonic Solution
CELL
(Salt Water) 10% NaCL90% H2O
(Salt Water)10% NaCL90% H2O
What is the direction of water movement?
The cell is at _______________.equilibrium
ENVIRONMENT
NO NET MOVEMENT
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Cell in Hypotonic Solution
CELL
(Salt Water) 10% NaCL90% H2O
(Salt Water)20% NaCL80% H2O
What is the direction of water movement?
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Cell in Hypertonic Solution
CELL
(Salt Water) 15% NaCL85% H2O
(Salt water)5% NaCL95% H2O
What is the direction of water movement?
ENVIRONMENT
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Isotonic Solution
NO NET MOVEMENT OF
H2O (equal amounts entering & leaving)
Hypotonic Solution
“Blow” –up
Think Hypo=Blow
Hypertonic Solution
Cell shrinks
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Osmosis in Red Blood Cells
Isotonic Hypotonic Hypertonic
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Three Forms of Transport Across the Membrane
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Passive Transport
1. Simple Diffusion
Doesn’t require energy
Moves high to lowconcentration Example: Oxygen or water diffusing into a cell and carbon dioxide diffusing out.
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Passive Transport
2. Facilitated diffusion
Doesn’t require energy
Uses transport proteinsto move high to lowconcentration
Examples: Glucose or amino acids moving from blood into a cell.
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Types of Transport Proteins
• Channel proteins are embedded in the cell membrane & have a pore for materials to cross
• Carrier proteins can change shape to move material from one side of the membrane to the other
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Facilitated DiffusionMolecules will randomly move through the pores in Channel Proteins.
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Facilitated Diffusion
• Some Carrier proteins do not extend through the membrane.
• They bond and drag moleculesthrough the lipid bilayer and release them on the opposite side.
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Active Transport
Requires energy or ATP
Moves materials from LOW to HIGHconcentration
AGAINSTconcentration gradient
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Active transport
Examples: Pumping Na+ (sodium ions) out and K+
(potassium ions) in against strong concentration gradients.
Called Na+-K+ Pump
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Sodium-Potassium Pump
3 Na+ pumped in for every 2 K+ pumped out; creates a membrane potential
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Pinocytosis- “Cell drinking”
Most common form of endocytosis.Takes in dissolved molecules as a vesicle.
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Endocytosis – Phagocytosis
Used to engulf large particles such as food, bacteria, etc. into vesicles
Called “Cell Eating”
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Molecules are moved out of the cell by vesicles that fusewith the plasma membrane.
Exocytosis- moving things out.
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Exocytosis
Inside Cell Cell environment
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Three Forms of Transport Across the Membrane
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Extra slides …
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ExocytosisExocytic vesicle immediately after fusion with plasma membrane.
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Moving the “Big Stuff”Large molecules move materials into the cell by
one of three forms of endocytosis.
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Pinocytosis
• Cell forms an invagination
• Materials dissolve in water to be brought into cell
• Called “Cell Drinking”
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Example of Pinocytosispinocytic vesicles forming mature transport vesicle
Transport across a capillary cell (blue).
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Receptor-Mediated Endocytosis
Some integral proteins have receptorson their surface to recognize & take in hormones, cholesterol, etc.
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Receptor-Mediated Endocytosis
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Phagocytosis About to Occur
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Phagocytosis- Capture of a YeastCell (yellow) by Membrane Extensions of an Immune System Cell(blue)
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Cells in Solutions
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DIFFUSION
Diffusion is a PASSIVE process which means no energy is used to make the molecules move, they have a natural KINETIC ENERGY
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Cytolysis & Plasmolysis
Cytolysis Plasmolysis
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hypotonic hypertonic isotonic
hypertonic isotonic hypotonic
What Happens to Blood Cells?
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Proteins Are Critical to Membrane Function
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Carrier Proteins
• Other carrier proteins change shapeto move materials across the cell membrane