the nucleotide sequence data are available in the ddbj/embl/genbank database
DESCRIPTION
Supplemental Table 1. Summary information for differentially expressed genes in tissue-cultured seedlings of mountain laurel with colonizing Streptomyces padanus AOK30, as revealed by suppression subtractive hybridization (SSH). - PowerPoint PPT PresentationTRANSCRIPT
No. Clone Putative function Accession E -value No. Clone Putative function Accession number E value No. Clone Putative function Accession E -value
Forward library 49 F115 Putative uncharacterized protein FY996736 3.3 26 R049 no hit FY996782 -
1 F003 Glutathione S-transferase. FY996694 6.00E-15 50 F116 Enolase (EC 4.2.1.11). FY996737 1.00E-
23 27 R050 S-adenosylmethionine decarboxylase 2 FY996783 9.00E-22
2 F005 Glutathione S-transferase. FY996695 3.00E-17 51 F119 no hit - - 28 R051 Alpha-glucosidase (EC 3.2.1.20). FY996784 1.00E-
16
3 F010 Similarity to endo-1,3-1,4-beta-d-glucanase FY996696 0.50 52 F120 Peroxidase FY996738 5.00E-15 29 R052 Cell wall hydrolase (Fragment). FY996785 2.00E-
14
4 F012 T30E16.25 (Glutathione S-transferase). FY996697 2.00E-20 53 F121 Putative uncharacterized protein. FY996739 4.00E-
10 30 R056 NAC-domain protein. FY996786 3.00E-04
5 F027 Glycosyl hydrolase family-like protein. FY996698 9.00E-78 54 F134 Glutathione S-transferase (Fragment). FY996740 6.00E-
15 31 R058 Putative uncharacterized protein FY996787 3.00E-56
6 F030 Similarity to similar to ubiquitin-conjugating enzyme FY996699 2.00E-
75 55 F152 Glutathione S-transferase. FY996741 3.00E-24 32 R059 UDP-glucose dehydrogenase-like protein FY996788 4.00E-
63
7 F031 UDP-glucose pyrophosphorylase (EC 2.7.7.9). FY996700 6.00E-28 56 F156 Putative uncharacterized protein. FY996742 2.00E-
05 33 R063 Allergenic isoflavone reductase-like protein FY996789 3.00E-50
8 F032 Putative uncharacterized protein. FY996701 9.00E-30 57 F162 Glutathione S-transferase FY996743 3.00E-
13 34 R064 Prohevein (Fragment). FY996790 8.00E-20
9 F033 Putative uncharacterized protein. FY996702 1.00E-31 58 F165 no hit - - 35 R065 UVB-resistance protein-like (At5g16040). FY996791 4.00E-
56
10 F034 WD-40 repeat-containing protein FY996703 2.00E-78 59 F167 Glucose-6P/phosphate translocator. FY996744 2.5 36 R066 SAG101. FY996792 6.00E-
22
11 F035 Polyprenyl synthetase. FY996704 5.6 60 F169 Malate dehydrogenase (EC 1.1.1.37). FY996745 2.00E-31 37 R067 Chromosome chr18 scaffold_1, whole genome... FY996793 8.00E-
82
12 F036 Asparagine synthase-rela ted protein. FY996705 1.00E-39 61 F170 no hit FY996746 - 38 R068 Sugar transporter ERD6-like 6. FY996794 1.00E-
53
13 F037 Glutathione S-transferase. FY996706 7.00E-19 62 F171 Catalase CAT1 (EC 1.11.1.6). FY996747 2.00E-
11 39 R070 Cyclase-like protein (Os09g0110300 protein). FY996795 4.00E-63
14 F044 Cysteine protease FY996707 4.00E-24 63 F172 BTB/TAZ domain protein FY996748 2.00E-
05 40 R071 Putative auxin-regulated protein. FY996796 6.00E-04
15 F046 no hit FY996708 - 64 F173 Putative 40S ribosomal protein FY996749 2.00E-08 41 R072 GDSL-like lipase/acylhydrolase family protein FY996797 7.2
16 F065 Caffeate O-methyltranfferase (Fragment) FY996709 0.034 65 F182 Protein CutA, chloroplast precursor (Copper... FY996750 5.00E-12 42 R075 Pyruvate/2-oxoglutarate dehydrogenase FY996798 5.9
17 F069 Putative uncharacterized protein. - 2.00E-20 66 F197 Ubiquitin-specific protease FY996751 8.00E-
12 43 R076 Putative uncharacterized protein. FY996799 9.9
18 F070 Glucan endo-1,3-beta-glucosidase, acidicis… FY996710 4.00E-10 67 F200 Non-specific lipid-transfer protein (Frag... FY996752 3.00E-
24 44 R077 Monooxygenase (Fragment). FY996800 8.00E-21
19 F072 no hit - - 68 F202 Multi antimicrobial extrusion protein MatE. FY996753 4.00E-14 45 R079 no hit FY996801 -
20 F073 Putative uncharacterized protein. FY996711 1.00E-09 69 F210 Peptidase S28. FY996754 2.00E-
20 46 R080 no hit FY996802 -
21 F075 Similarity to protein kinase. FY996712 3.4 70 F234 no hit FY996755 - 47 R082 Pathogenesis-related protein, PR-3 type FY996803 7.00E-19
22 F076 no hit FY996713 - 71 F239 Putative cinnamyl-alcohol dehydrogenase; ... FY996756 0.016 48 R083 60S ribosomal protein L18a-2. FY996804 2.00E-14
23 F078 no hit FY996714 - Reverse library 49 R084 Putative uncharacterized protein At4g0029... - 2.00E-04
24 F079 Putative uncharacterized protein FY996715 7.00E-16 1 R003 Putative uncharacterized protein. FY996757 1.00E-
33 50 R085 Carbamoyl-phosphate synthase, small subunit. FY996805 2.00E-29
25 F080 no hit - - 2 R004 Triglyceride lipase FY996758 4.00E-36 51 R086 no hit FY996806 -
26 F081 Similar to SOUL Protein (At1g17100). FY996716 0.009 3 R005 Tetratricopeptide repeat domain 5-like protein FY996759 0.85 52 R089 H-ATPase FY996807 7.00E-19
27 F084 Glutathione S-transferase GST 9 FY996717 0.009 4 R006 26S proteasome subunit RPN6a FY996760 5.00E-66 53 R090 Predictec protein FY996808 2.00E-
19
28 F086 Ribulose bisphosphate carboxylase small c… FY996718 3.4 5 R007 Putative uncharacterized protein. FY996761 7.6 54 R091 Putative basic-helix-loop-helix (BHLH) FY996809 0.23
29 F087 Trypsin-like serine protease. FY996719 4.3 6 R011 Flavonoid 3’ -hydroxylase FY996762 5.00E-12 55 R097 Putative uncharacterized protein. FY996810 7.00E-
11
30 F088 no hit FY996720 - 7 R013 Putative uncharacterized protein. FY996763 0.081 56 R098 no hit FY996811 -
31 F089 3-isopropylmalate dehydratase, small subu... FY996721 3.00E-04 8 R014 Putative phosphatase FY996764 2.00E-
17 57 R099 no hit - -
32 F092 no hit - - 9 R016 AAA-metalloprotease FtsH. FY996765 1.00E-44 58 R103 Dehydrin. FY996812 5.00E-
04
33 F093 Similarity to GTPase activating protein FY996722 2.00E-14 10 R019 Chitinase, class V FY996766 7.00E-
24 59 R105 no hit - -
34 F094 Putative uncharacterized protein FY996723 4.00E-28 11 R020 Putative uncharacterized protein. FY996767 4.00E-
05 60 R106 Chitinase CHI1. - 0.046
35 F095 Diphosphonucleotide phosphatase 1 FY996724 2.00E-21 12 R021 Oxygen-evolving enhancer protein 2 FY996768 1.00E-
37 61 R107 Actin (Fragment). FY996813 1.00E-10
36 F096 F3F20.14 protein (BTB and TAZ domain protein… FY996725 2.00E-05 13 R024 Stress and pathogenesis-related protein. FY996769 9.00E-
11 62 R112 Histidine-containing phosphotransfer protein FY996814 1.00E-05
37 F097 no hit - - 14 R027 Stress-related protein FY996770 5.00E-26 63 R113 Putative uncharacterized protein At5g08450. FY996815 2.00E-
28
38 F100 no hit FY996726 - 15 R028 Putative uncharacterized protein. FY996771 5.00E-34 64 R114 Putative 1-acyl-sn-glycerol-3-phosphate… FY996816 5.00E-
09
39 F101 no hit FY996727 - 16 R031 no hit FY996772 - 65 R120 Trehalose-6-phosphate synthase homolog. FY996817 3.00E-17
40 F102 NADH-ubiquinone oxidoreductase 75 kDa sub... FY996728 8.00E-13 17 R034 Plastidic ATP/ADP-transporter. FY996773 5.00E-
36 66 R123 Putative uncharacterized protein. FY996818 1.00E-23
41 F103 Dual specificity protein phosphatase fami... FY996729 7.00E-22 18 R035 Proline-rich family protein-like FY996774 5.00E-
18 67 R124 Probable metal-nicotianamine transporter... FY996819 2.00E-25
42 F104 no hit FY996730 - 19 R036 At1g10410/F14N23_31. FY996775 8.00E-45 68 R125 MFS family transporter: sugar (Sialic acid). FY996820 7.5
43 F107 no hit FY996731 - 20 R039 Stress and pathogenises-related protein FY996776 9.00E-11 69 R126 Putative hydrolase. FY996821 8.00E-
31
44 F108 Putative Na+/myo-inositol symporter. FY996732 6.00E-13 21 R040 Glycosyl transferase, family 48 (Fragment). FY996777 7.00E-
16 70 R127 Coated vesicle membrane protein-like. FY996822 1.00E-07
45 F109 no hit FY996733 - 22 R042 Putative senescence-associated protein FY996778 7.00E-14 71 R128 Formamidase-like protein. - 2.00E-
08
46 F111 no hit - - 23 R044 Poly(A)-binding protein (Fragment). FY996779 2.00E-26 72 R129 Hypersensitive-induced response protein. FY996823 3.00E-
08
47 F112 no hit FY996734 - 24 R047 Vegetative cell wall protein gp1-like FY996780 9.00E-25 73 R130 histone H3 FY996824 3.00E-
21
48 F113 NADH-dependent glutamate synthase precurs... FY996735 1.00E-14 25 R048 AG-motif binding protein-2. FY996781 1.00E-
07 74 R131 Heat shock protein. FY996825 1.00E-08
The nucleotide sequence data are available in the DDBJ/EMBL/GenBank database.
Supplemental Table 1. Summary information for differentially expressed genes in tissue-cultured seedlings of mountain laurel with colonizing Streptomyces padanus AOK30, as revealed by suppression subtractive hybridization (SSH)
No. Clone Putative function Accession E -value
Reverse library (Continued)
75 R139 Class IV chitinase (CHIV) (At3g54420). FY996826 0.68
76 R141 Putative uncharacterized protein. FY996827 10
77 R143 Prolyl-tRNA synthetase (Fragment). - 0.025
78 R144 no hit FY996828 -
79 R145 no hit FY996829 -
80 R149 Protein with 2 possible TPR domains FY996830 9.6
81 R156 Valyl-tRNA synthetase, putative FY996831 6.00E-05
82 R158 no hit FY996832 -
83 R163 Maturase K (Fragment). FY996833 7.4
84 R165 no hit FY996834 -
85 R166 no hit - -
86 R171 no hit - -
87 R173 no hit FY996835 -
88 R174 CPRD2 protein. FY996836 0.002
89 R178 AAA-type ATPase-like protein. FY996837 4.00E-16
90 R179 Putative calcium-dependent protein kinase… FY996838 2.00E-
14
91 R184 Protein translation factor FY996839 2.00E-26
92 R186 Ribonuclease Z, chloroplast precursor FY996840 2.00E-20
93 R190 Non-cell-autonomous heat shock cognate FY996841 3.00E-40
94 R194 Putative uncharacterized protein FY996842 3.00E-16
95 R195 no hit - -
96 R196 no hit - -
97 R197 Putative uncharacterized protein. FY996843 7.9
98 R202 Metallothionein-like protein FY996844 8.00E-18
99 R203 Snakin2 precursor. FY996845 1.00E-09
100 R206 Putative serine/threonine protein kinase. FY996846 6.1
101 R211 no hit - -
102 R218 Ataxin-2 related protein. FY996847 9.7
103 R219 Putative calcium-transporting ATPase. FY996848 4.00E-12
104 R220 Methionine synthase (Fragment) FY996849 2.00E-15
105 R221 Pore-forming toxin-like protein Hfr-2. FY996850 0.23
106 R226 no hit FY996851 -
107 R227 Phi-1 protein. FY996852 2.00E-07
108 R231 Metallothionein2. FY996853 3.00E-20
109 R236 Metallothionein-like. FY996854 2.00E-16
Supplemental Table 1 continued. Summary of differentially expressed genes in tissue-cultured seedlings of mountain laurel with colonizing Streptomyces padanus AOK30, as revealed by suppression subtractive hybridization (SSH) procedure.