the microscope agricultural biology. the microscope two major types of microscopes based on energy...
TRANSCRIPT
The Microscope
Two major types of microscopes based on energy used by device:
1. Light microscope• Uses visible light that is magnified as it passes
through glass lenses• Source: Light• Magnifies up to 2000 times by using glass lenses• Materials can be living or dead
The Microscope
Two major types of microscopes based on energy used by device:
2. Electron microscope• Electron beam passes through magnetic lenses
before magnified image is projected on photographic film or a fluorescent screen similar to television.
• Source: Electrons• Magnifies up to 2 million times by using magnets• Materials should be dead
The Microscope
• You will use the compound microscope– Why is it “compound”?
• Two lenses collect and focus the light from the source as it is transmitted through the sample.
• For light to pass through the specimen, the specimen must be very thin.
• The image of the specimen seen in the microscope is not only magnified but the resolution is improved.
The Microscope
• Resolution: Ability to distinguish and separate fine details in a specimen, such as two objects that are close to each other as being two rather than one– Our eyes can “resolve” two objects that are at
least 0.1 millimeters (mm) apart
– A good light microscope has a resolution up to 1000 times closer or 0.1 micrometers (µm)
The Microscope
• Contrast: Ability to distinguish detail; different absorption of light by parts of the object being viewed–Can be increased by decreasing light
intensity or by staining of the specimen• Proper selection of stains also increases
our ability to identify specific structures within specimen
Microscopic Terms• Slide: Piece of glass upon which
specimen is placed for viewing.
• Cover Slip: Thin square piece of glass or plastic used to cover specimen.
• Field of View: Area of specimen which is seen through lens system; bright circle you see when looking into a microscope.
Microscopic Terms• Depth of Field: Ability of lens to furnish
distinct image above and below focal plane. Decreases with increase of magnification.
• Parfocal: When objective is in focus, it can be switched from one power to another, with only small fine focus adjustments necessary
Microscopic Terms• Magnification: Number of times an object’s
apparent size is increased by lens system– Determined by multiplying power of objective by
the power of eyepiece (usually 10x).
Power of objective
x Power of eyepiece (usually 10x)
Total magnification
Microscopic Terms• High Power: High power objective on
nosepiece (usually 40x).• Medium Power: Middle power
objective on nosepiece (usually 10x).• Low Power: Lowest power objective
on nosepiece (usually 4x).
Use of the Microscope1. Carry with both hands.
2. Set microscope in from edge of desk.
3. Use lens paper to clean eyepiece and objectives.
4. Start with lowest power (shortest objective) in place.
Use of the Microscope
5. Turn coarse adjustment so that stage is as close to low power as possible -- now while looking, turn coarse adjustment so stage starts to move away from objective.
6. Clean up all spills and moisture from stage before putting away.
Use of the Microscope
7. Caution – Keep all water away from electrical parts
8. Never use coarse adjustment with high power objective while trying to focus, because of danger of striking and breaking glass slide with objective.
Functions of Microscope Parts• Ocular Lenses:
Upper lenses nearest to eyes; magnify image already magnified by objective; brings light rays to a focus at the eye
Functions of Microscope Parts• Rotating Nose
Piece: Revolving part that supports three objectives with different magnifications
Functions of Microscope Parts• Objective Lens:
Lens closest to specimen; magnifies image of specimen
Functions of Microscope Parts• Iris Diaphragm
Lever:
Disc-shaped device, located beneath stage, controls amount of light passing through specimen
Functions of Microscope Parts
• Thumb Clip: Hold slide on stage, to prevent it from slipping around.
Functions of Microscope Parts• Coarse
Adjustment Knob: Roughly focuses image by bringing the specimen and objective lenses closer together or farther apart.
Functions of Microscope Parts• Fine Adjustment
Knob: Sharpens image of specimen by moving specimen and objective lenses very small distances closer together or farther apart.