the method of intravenous laser blood irradiation and ... · the blue laser irradiation with blue...
TRANSCRIPT
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The method of intravenouslaser blood irradiation and
clinical applications
The method of intravenouslaser blood irradiation and
clinical applications
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IV Laser Blood Irradiation
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UV-radiation of blood
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Applied of the catheter in the blood stream
-
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IV Lasertreatment with
Red and green laser
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Iv-laser treatment with blue and yellowlaser
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Puncture needles for intravenous lasertherapy
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Y-needle with 3 x Luer-lock for simultaneousinfusion therapy
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Intravenöse Laserblutbehandlung mitverschiedenen Wellenlängen:
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Red laser intravenous therapy
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The laser-needle mouth shower
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The laser-needle mouth shower for systemicsublingual laser energy application
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Weberneedle 12-channel modularEndolaser system
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The intravenous laser blood irradiation fordiabetes
(blood acupuncture)
Diabetes mellitus with leg ulcer
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The intravenous laser blood irradiationfor allergy (blood acupuncture)
Treatment of severe allergie withcombined laser therapyTreatment of severe allergie withcombined laser therapy
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Effects of intravenous laser light irradiation
Red laserStimulation of the immune system, improvement ofblood viscosity
Green laser
Increased oxygen supply
Blue laser:Increased NO, bactericidal effects
Ultraviolett laser:Kills viruses and bacteria
Yellow laser:Detoxification, antidepressive
Red laserStimulation of the immune system, improvement ofblood viscosity
Green laser
Increased oxygen supply
Blue laser:Increased NO, bactericidal effects
Ultraviolett laser:Kills viruses and bacteria
Yellow laser:Detoxification, antidepressive
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Effects of intravenous laser bloodirradiation on mitochondria
„Giant-mitochondria“ in humanlymphocytes after laserirradiation (632 nm)
Ring-shaped mitochondria inhuman lymphocytes after laserirradiation (632nm)
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Increased ATP production
ATP-Increase under laser irradiation (632 nm, red light) of aHeLa cell-culture
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Immunological effects of iv-Laser
Activation of macrophages in fluorescent light
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Immunological effects of iv-Laser
Laserclinic Dr. med. Dipl. chem.Michael Weber, Germany
Mouayed A. Hasan et al., Estimation of IgM & IgG values in the serum after intravenousirradiation of blood with diode laser
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Immunological effects of iv-Laser
Laserclinic Dr. med. Dipl. chem.Michael Weber, Germany
Mouayed A. Hasan et al., Estimation of IgM & IgG values in the serum after intravenousirradiation of blood with diode laser
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Intravenous green laser
Green laserlight binds to haemoglobin
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Effects of the green Laser on mitochondria
Gen Physiol Biophys. 2005 Jun;24(2):209-20.
Mitochondrial alterations induced by 532 nm laser irradiation.
Kassak P, Przygodzki T, Habodaszova D, Bryszewska M, Sikurova L.
Division of Biomedical Physics, Faculty of Mathematics, Physics and Informatics,Comenius University, Mlynska Dolina F1, 842 48 Bratislava 4, Slovakia.
Another MTT assay was used for isolated mitochondria suspensions in order toexamine the effect of green laser irradiation on stimulation of processes related tooxidative phosphorylation. It revealed 31.3%-increase in MTT assay products inirradiated mitochondria as compared to controls
Green laserlight increases the production of ATP in theirradiated mitochondria for more than 30%.
Gen Physiol Biophys. 2005 Jun;24(2):209-20.
Mitochondrial alterations induced by 532 nm laser irradiation.
Kassak P, Przygodzki T, Habodaszova D, Bryszewska M, Sikurova L.
Division of Biomedical Physics, Faculty of Mathematics, Physics and Informatics,Comenius University, Mlynska Dolina F1, 842 48 Bratislava 4, Slovakia.
Another MTT assay was used for isolated mitochondria suspensions in order toexamine the effect of green laser irradiation on stimulation of processes related tooxidative phosphorylation. It revealed 31.3%-increase in MTT assay products inirradiated mitochondria as compared to controls
Green laserlight increases the production of ATP in theirradiated mitochondria for more than 30%.
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Stimulation of sodium-potassium-ATP-ase of humanerythrocytes with green laser irradiation
Kassak et al, Univ. Bratislava und Lodz, die Reaktion der Na + / K + - ATPasemenschlicher Erythrozyten zu grünem Laserlicht Behandlung; Phys. Res. 5 / 2005
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First yellow laser worldwide:
• after the development of red, infrared, greenand blue lasers, yellow was the last missingprismatic color
• yellow additionally stimulates themitochondrial respiratory chain at complexIII (cytochromes)
• yellow has an detoxifying effect• yellow has an anti- depressive effect• The yellow laser stimulates the strongest
natural photosensitizer – Hypericin out ofSt. Johns wort – and is therefore one of themost efficient laser in photodynamic cancertherapy.
• after the development of red, infrared, greenand blue lasers, yellow was the last missingprismatic color
• yellow additionally stimulates themitochondrial respiratory chain at complexIII (cytochromes)
• yellow has an detoxifying effect• yellow has an anti- depressive effect• The yellow laser stimulates the strongest
natural photosensitizer – Hypericin out ofSt. Johns wort – and is therefore one of themost efficient laser in photodynamic cancertherapy.
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Application of blue laser light
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The new 447nm Blue Laser
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The blue Laser
Irradiation with blue laser leads to increase of the release of nitric oxide(NO) from haemoglobin
Kinetics of NO release and reabsorption triggered by He-Cd laser (40 mW) irradiation in erythrocytesenriched with NO-Hb.
(A) Anaerobic irradiation; (B) Aerobic irradiation; (C) Difference in NO concentration in solution dueto switching the laser ON and OFF.
Mittermayr et al., Ludwig Boltzmann Institut Wien in Zusammenarbeitmit der Russian State Medical University in MoskauMol Med. 2007 Jan-Feb; 13 (1-2): 22-29
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Blue laser increases nitric oxide (NO)
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Blue laser increases nitric oxide (NO)
• Emerging evidence suggests that increasing nitric oxide(NO) bioavailability or endothelial NO synthase (eNOS)activity activates telomerase and delays endothelial cellsenescence.
J Cell Sci. 2006 Jul 15;119(Pt 14):2855-62.
• Emerging evidence suggests that increasing nitric oxide(NO) bioavailability or endothelial NO synthase (eNOS)activity activates telomerase and delays endothelial cellsenescence.
J Cell Sci. 2006 Jul 15;119(Pt 14):2855-62.
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Blue laser increases nitric oxide (NO)
• J Cell Sci. 2006 Jul 15;119(Pt 14):2855-62.
Nitric oxide and mitochondrialbiogenesis.
Chronic, smaller increases in NO levels stimulate
mitochondrial biogenesis in diverse cell types
• J Cell Sci. 2006 Jul 15;119(Pt 14):2855-62.
Nitric oxide and mitochondrialbiogenesis.
Chronic, smaller increases in NO levels stimulate
mitochondrial biogenesis in diverse cell types
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Mikrocirculation problems in
- Macroangiopathy, Microangiopathy
- Diabetes mellitus
- coronary heart disease
- Fat metabolism disturbances
- Hypertension
- Kidney failure
- Old humans
- After transplantations
NO is in the view of today the main physiolocical regulator of themicrocirculation and is infuencing the cGMP-metabolism.
In the blood NO is not free but will bind immediately to haemoglobin.
Der HbNO-complex is photosensitive and reacts on laser irradiation.
The Blue laser
Laserclinic Dr. med. Dipl. chem.Michael Weber, Germany
Mikrocirculation problems in
- Macroangiopathy, Microangiopathy
- Diabetes mellitus
- coronary heart disease
- Fat metabolism disturbances
- Hypertension
- Kidney failure
- Old humans
- After transplantations
NO is in the view of today the main physiolocical regulator of themicrocirculation and is infuencing the cGMP-metabolism.
In the blood NO is not free but will bind immediately to haemoglobin.
Der HbNO-complex is photosensitive and reacts on laser irradiation.
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The blue laser in ENT
Patient, 45 y., acute hearing loss, medication without effect
Improvement ca. 50 % after 4 sessions
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Effects on Telomeres
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Effects on Telomeres
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Typical diseases to treat with laser bloodirradiation
Fibromyalgia
Hypertension
Tinnitus
Macula degeneration
Multiple Sclerosis
Chronic fatigue syndrome
allergies and eczemas
Diabetes mellitus
Chronic liver diseases
Lipometabolism disorders
Chronic pain syndromes
Rheumatoid Arthritis
Polyneuropathy
Chronic inflammatory diseases
Cancer (photodynamic therapy)
Laserclinic Dr. med. Dipl. chem.Michael Weber, Germany
Fibromyalgia
Hypertension
Tinnitus
Macula degeneration
Multiple Sclerosis
Chronic fatigue syndrome
allergies and eczemas
Diabetes mellitus
Chronic liver diseases
Lipometabolism disorders
Chronic pain syndromes
Rheumatoid Arthritis
Polyneuropathy
Chronic inflammatory diseases
Cancer (photodynamic therapy)
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General effects:
- Improvement of the general performance
- Improved Sleep
- Positive effect on depression
- improvement of the immune system
The intravenous laser blood irradiation
Laserclinic Dr. med. Dipl. chem.Michael Weber, Germany
General effects:
- Improvement of the general performance
- Improved Sleep
- Positive effect on depression
- improvement of the immune system
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Regenerative effects of intravenous laser therapy
• There is strong evidence that theregenerative effects of intravenous lasertherapy are induced by stimulation of thebody‘s own stem cells released from thebone marrow in the blood stream
• There is strong evidence that theregenerative effects of intravenous lasertherapy are induced by stimulation of thebody‘s own stem cells released from thebone marrow in the blood stream
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Studies
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Application in cardiology and angiology
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Before Lasertherapy
After Lasertherapy
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Cardiovascular Disease• Includes anything adversely affecting heart
and blood vessels such as:
- Angina / Ischemic Heart Disease- Atherosclerosis- Dysrhythmia- Hypertension- Hyperlipidemia- Myocardial Infarction- Stroke- …and more
• Includes anything adversely affecting heartand blood vessels such as:
- Angina / Ischemic Heart Disease- Atherosclerosis- Dysrhythmia- Hypertension- Hyperlipidemia- Myocardial Infarction- Stroke- …and more
1) Rosamond W, et al. Heart disease and stroke statistics 2007 update. A report from the American Heart Association StatisticsCommittee and Stroke Statistics Subcommittee. Circulation 2006; DOI: 10.1161/ circulationaha.106.179918.
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Widespread effects onBlood & Blood Vessels
• Reduced aggregation of platelets.• Increased elasticity/deformability of red cells.• Reduced viscosity / improved microcirculation.• Reduced coagulability.• Increased antioxidant levels.• Increased oxygen binding to red blood cells.• Prevention, even regression of plaque.• Immune activation.• Increase of kidney function
• Reduced aggregation of platelets.• Increased elasticity/deformability of red cells.• Reduced viscosity / improved microcirculation.• Reduced coagulability.• Increased antioxidant levels.• Increased oxygen binding to red blood cells.• Prevention, even regression of plaque.• Immune activation.• Increase of kidney function
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Study about the efficacy of laser therapy onpatients with coronary heart disease
F. Noohi, MD. FACC , M. Javdani, MD*, M. kiavar, MDShaheed Rajaei Cardiovascular Medical & Research Center. IRAN
University of Medical Science, Tehran, IRAN, Nov.2008
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stage
post irradiationpre irradiation
SIXMINUTE
500
400
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stage
post irradiationpre irradiation
SIXMINUTE
500
400
300
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B
6 Minuten Lauftest
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stage
post irradiationpre irradiation
EF
50
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stage
post irradiationpre irradiation
EF
50
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10C
Ejektionsfraktion des Herzens
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The effect of intravenous laser on metabolism anddiabetes
Die Dynamik des Lipidprofils (mmol/l bei Patienten mit Diabetes mellitus (M ±m)
I= Kontrollgruppe (n=22) ohne CLT- Behandlung
II= Hauptgruppe (n=37) mit Behandlung
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Blutzuckerwerte (M ±m)
I= Kontrollgruppe (n=30) ohne CLT- Behandlung
II= Hauptgruppe (n=37) mit Behandlung
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Evaluating the Efficiency of Low Level Laser Therapy (LLLT)in Combination With Intravenous Laser Therapy (IVL) onDiabetic Foot Ulcer, Added to Conventional Therapy
Soheila Mokmeli MD1, Mahrokh Daemi MD2, Zahra Ayatollahzadeh Shirazi MD1Fatemah Ayatollahzadeh Shirazi PhD3, Mitra Hajizadeh MD4
1Department of Medical Laser, Milad Hospital, Social SecurityOrganization, Tehran, Iran2Department of Surgery, Sina Hospital,Tehran University ofMedical Sciences, Tehran, Iran
Evaluating the Efficiency of Low Level Laser Therapy (LLLT)in Combination With Intravenous Laser Therapy (IVL) onDiabetic Foot Ulcer, Added to Conventional Therapy
Soheila Mokmeli MD1, Mahrokh Daemi MD2, Zahra Ayatollahzadeh Shirazi MD1Fatemah Ayatollahzadeh Shirazi PhD3, Mitra Hajizadeh MD4
1Department of Medical Laser, Milad Hospital, Social SecurityOrganization, Tehran, Iran2Department of Surgery, Sina Hospital,Tehran University ofMedical Sciences, Tehran, Iran
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Diabetes mellitus, metabolic syndrome
56
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New Diabetes study 2008( Dr. Andreas Wirz, Basel, Switzerland)
• Protocol: 100 diabetic patients were treatedwith 10 sessions red and green lasersintravenously with the new insulinefrequency of 3023 Hz
• Results: positive effects in 75 %Reduction of HbA1c of 1,5 %( this study was presented at the international congress foracupuncture in Davos, Switzerland, February 2008, will be publishedsoon )
• Protocol: 100 diabetic patients were treatedwith 10 sessions red and green lasersintravenously with the new insulinefrequency of 3023 Hz
• Results: positive effects in 75 %Reduction of HbA1c of 1,5 %( this study was presented at the international congress foracupuncture in Davos, Switzerland, February 2008, will be publishedsoon )
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The Hypoglycemic Effect of Intravenous LaserTherapy in Diabetic Mellitus Type 2 Patients;A Systematic Review and Meta-analyses
Kazemikhoo N1, 2, Ansari F2 and Nilforoushzadeh21 1Skin Diseases and Leshmaniasis Research Center, Isfahan University of MedicalSciences, Isfahan, Iran 2 Skin and Stem Cell Research Center, Tehran University ofMedical Sciences, Tehran, Iran12/2015
Kazemikhoo N1, 2, Ansari F2 and Nilforoushzadeh21 1Skin Diseases and Leshmaniasis Research Center, Isfahan University of MedicalSciences, Isfahan, Iran 2 Skin and Stem Cell Research Center, Tehran University ofMedical Sciences, Tehran, Iran12/2015
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R. Chen, 2000 (Chen, Chen, Xie, Chen, & Zhang, 2000) 1067.3 93.3He-Ne laser extravacular irradiation therapy instrument,O—4Omw, 632.8nm, 60 min197.1±73.8 106.2±540
T.V. Kovalyova, 2002 (Kovalyava, 2002) 27 57.3 13ILBI intravenously 2 mW,l =0,63 mm 405-nm 15-30 min259.74±15.48 255.78±15.3
N. KazemiKhoo, 2013 (N Kazemi Khoo et al., 2013) 9 60.6355ILBI intravenously 1.5 mW, continuous, 405-nm 30 min190±17 165±20
N. KazemiKhoo, 2015 (KazemiKhoo & Ansari, 2015) 24 3763.7ILBI intravenously 1.5 mW, continuous, 405-nm and 630 nm20-30min-one day blue one day red laser therapy214.71±78.99 188.53±75.60
Studies
R. Chen, 2000 (Chen, Chen, Xie, Chen, & Zhang, 2000) 1067.3 93.3He-Ne laser extravacular irradiation therapy instrument,O—4Omw, 632.8nm, 60 min197.1±73.8 106.2±540
T.V. Kovalyova, 2002 (Kovalyava, 2002) 27 57.3 13ILBI intravenously 2 mW,l =0,63 mm 405-nm 15-30 min259.74±15.48 255.78±15.3
N. KazemiKhoo, 2013 (N Kazemi Khoo et al., 2013) 9 60.6355ILBI intravenously 1.5 mW, continuous, 405-nm 30 min190±17 165±20
N. KazemiKhoo, 2015 (KazemiKhoo & Ansari, 2015) 24 3763.7ILBI intravenously 1.5 mW, continuous, 405-nm and 630 nm20-30min-one day blue one day red laser therapy214.71±78.99 188.53±75.60
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Macular degeneration
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Application in macular degeneration
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Results
01020304050607080
MD1 MD3 RP1 RP3
cohort
Overall results
Percentage gainedPercentage seen "before"
01020304050607080
MD1 MD3 RP1 RP3
cohort
Overall results
Percentage gainedPercentage seen "before"
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INTRAVENOUS LASERINTRAVENOUS LASERBLOOD IRRADIATIONBLOOD IRRADIATIONIN SPORTS MEDICINEIN SPORTS MEDICINE
INTRAVENOUS LASERINTRAVENOUS LASERBLOOD IRRADIATIONBLOOD IRRADIATIONIN SPORTS MEDICINEIN SPORTS MEDICINE
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Materials and MethodsMaterials and Methods• Definitions
– Maximum strength tests:1. Pectoral muscle maximum lifting power
» Weight is lift in supine position, while athlete lies onthe bench
» Maximum weight has to be confirmed by 8 followinglifting of sub-maximal weight (80% of maximum)
• Definitions– Maximum strength tests:
1. Pectoral muscle maximum lifting power» Weight is lift in supine position, while athlete lies on
the bench» Maximum weight has to be confirmed by 8 following
lifting of sub-maximal weight (80% of maximum)
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Maximum strength tests:Pectoral muscle maximum lifting power
% variation (mean)
ResultsResults
0,00
11,45 11,1712,29
10,34
7,26
-1,12
-2,00
0,00
2,00
4,00
6,00
8,00
10,00
12,00
14,00
beforetreatment
aftertreatment
4 weeksafter end
8 weeksafter end
12 weeksafter end
16 weeksafter end
20 weeksafter end
%
0,00
11,45 11,1712,29
10,34
7,26
-1,12
-2,00
0,00
2,00
4,00
6,00
8,00
10,00
12,00
14,00
beforetreatment
aftertreatment
4 weeksafter end
8 weeksafter end
12 weeksafter end
16 weeksafter end
20 weeksafter end
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Materials and MethodsMaterials and Methods• Definitions
– Endurance tests:1. Cord jumping time
» Is the maximum time that athlete could perform incord jumping until he felt tired
• Definitions– Endurance tests:
1. Cord jumping time» Is the maximum time that athlete could perform in
cord jumping until he felt tired
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Endurance tests:Cord Jumping time % variation (mean)
ResultsResults
0,00
83,3390,48 88,10
95,2485,71
0,000102030405060708090
100
beforetreatment
aftertreatment
4 weeksafter end
8 weeksafter end
12 weeksafter end
16 weeksafter end
20 weeksafter end
%
0,00
83,3390,48 88,10
95,2485,71
0,000102030405060708090
100
beforetreatment
aftertreatment
4 weeksafter end
8 weeksafter end
12 weeksafter end
16 weeksafter end
20 weeksafter end
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Race camels in Dubai
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Endurance sport
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Sheik Nasser, Prince of Bahrain
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New Developments inRegenerative Medicine:
Interstitial laser therapy,PRP, Stem cells, intravenous laser and
cosmetic laser therapy
New Developments inRegenerative Medicine:
Interstitial laser therapy,PRP, Stem cells, intravenous laser and
cosmetic laser therapy
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Absorption of laser light in biological tissue
532nm,589 635,658nm 810nm405,447
green red Infrared
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The skin barrier
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Disturbing effects of laser penetration in biological tissue
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Optical penetration depth of different wavelengths
- depends upon the wavelength- Tissue penetration of blue laser very low, green
laser ca. 5mm, red 3 cm, infrared 6 cm
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The structure of the mitochondria canbe different in the special types oftissue cells.
In living cells mitochondria have adynamic structure; this means that theycan vary their structure and size. Theyare able to merge or to dividethemselves.
The mitochondria are making outabout 10 – 15 % of the volume of aliving cell.
Their main task is the production ofATP
The mitochondria have an inner andouter membrane.
In the inner room of the mitochondriawe can find the christae, formed bydouble layer membranes, where therespiratory chain is located and theproduction of ATP.
The structure of the mitochondria
Laserclinic Dr. med. Dipl. chem. MichaelWeber, Germany
The structure of the mitochondria canbe different in the special types oftissue cells.
In living cells mitochondria have adynamic structure; this means that theycan vary their structure and size. Theyare able to merge or to dividethemselves.
The mitochondria are making outabout 10 – 15 % of the volume of aliving cell.
Their main task is the production ofATP
The mitochondria have an inner andouter membrane.
In the inner room of the mitochondriawe can find the christae, formed bydouble layer membranes, where therespiratory chain is located and theproduction of ATP.
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The respiratory chain in the mitochondria
Karp, Molekulare Zellbiologie
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The respiratory chain in the mitochondria
Karp, Molekulare Zellbiologie
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The respiratory chain in the mitochondria
In figure 13 we find the processes of energy production in the mitochondria.
We should remember again that with the blue laser we will stimulate the starter complex NADH-dehydrogenase and with the red and infrared laser the end-complex cytochrome-c-oxydase.
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The intraarticular laser therapy
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The anatomy of the shoulder
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Shoulder syndrome
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Shoulder syndrome
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Intraarticular laser therapy
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Interstital laser therapy for spine syndromes
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Interstitial laser therapy for spine syndromes
Laserclinic Dr. med.Dipl. chem. Michael
Weber, Germany
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Step 1:Local anaesthesia
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NaCl for improvement of beam spreading
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Interstitial fiberoptic canula ( 4,5,8,10,12 cm )
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New blue laser 447 nm
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Blue laser increases nitric oxide (NO)
• J Cell Sci. 2006 Jul 15;119(Pt 14):2855-62.
Nitric oxide and mitochondrialbiogenesis.
Chronic, smaller increases in NO levels stimulate
mitochondrial biogenesis in diverse cell types
• J Cell Sci. 2006 Jul 15;119(Pt 14):2855-62.
Nitric oxide and mitochondrialbiogenesis.
Chronic, smaller increases in NO levels stimulate
mitochondrial biogenesis in diverse cell types
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Blue laser increases nitric oxide (NO)
• Emerging evidence suggests that increasing nitric oxide(NO) bioavailability or endothelial NO synthase (eNOS)activity activates telomerase and delays endothelial cellsenescence.
J Cell Sc. 2006 Jul 15;119(Pt 14):2855-62.
• Emerging evidence suggests that increasing nitric oxide(NO) bioavailability or endothelial NO synthase (eNOS)activity activates telomerase and delays endothelial cellsenescence.
J Cell Sc. 2006 Jul 15;119(Pt 14):2855-62.
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Sterile fiberoptics with circualrirradiation
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Sterile fiberoptics with spheric irradiation
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Step 3:Inserted catheter for interstitial laser therapy
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Interstitial laser application
• Blue and yellow laser
• Cooling effect
• Antiinflammatory effect
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Spinal interstitial laser therapy
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OA is a disease ofjoints that affects allof the weight-bearingcomponents of thejoint:
•Articular cartilage•Menisci•Bone
The intraarticular laser therapy for kneeosteoartrits
OA is a disease ofjoints that affects allof the weight-bearingcomponents of thejoint:
•Articular cartilage•Menisci•Bone
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•Periarticularsclerosis
•Osteophytes
•Sub-chrondralbone cysts
Advanced knee osteoarthrits
•Periarticularsclerosis
•Osteophytes
•Sub-chrondralbone cysts
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Fiberoptic cannulas for intraarticular lasertherapy
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Fiberoptic plastic cannula
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The intraarticular laser therapy
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Fiberoptic butterfly for small joints
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The intraarticular laser therapy
Laserclinic Dr. med. Dipl. chem.Michael Weber, Germany
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The intraarticular laser therapy
Laserclinic Dr. med. Dipl. chem.Michael Weber, Germany
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The intraarticular laser therapy
Laserclinic Dr. med. Dipl. chem.Michael Weber, Germany
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Intraarticular laser in Shoulder syndromespresented on World of Pain conference, Miami 2012
Number ofpatients = 15
Number oftreatments meanvalue 9,40
• VAS before6,67• VAS after 3,33
(dose about 1 J)
4
5
6
7
8
9
10
Pain on a10-point-scale Pain before treatment
Pain after treatment
Number ofpatients = 15
Number oftreatments meanvalue 9,40
• VAS before6,67• VAS after 3,33
(dose about 1 J)
0
1
2
3
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Patients
Pain after treatment
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Intraarticular laser in knee syndromespresented on wold of Pain conference, Miami 2012
Number ofpatients = 11
Number oftreatments meanvalue 6
• VAS before7,27• VAS after 3,36
(dose about 10J)
4
5
6
7
8
9
10
Pain on a10-point-scale Pain before treatment
Pain after treatment
Number ofpatients = 11
Number oftreatments meanvalue 6
• VAS before7,27• VAS after 3,36
(dose about 10J)
0
1
2
3
4
1 2 3 4 5 6 7 8 9 10 11
Patients
Pain after treatment
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Interstitial laser in spine syndromespresented on world of Pain conference Miami 2012
Number ofpatients = 23
Number oftreatments meanvalue 8,13
• VAS before6,48• VAS after 3,78
(dose about 10J)
6
8
10
12
Pain on a10-point-scale Pain before treatment)
Number ofpatients = 23
Number oftreatments meanvalue 8,13
• VAS before6,48• VAS after 3,78
(dose about 10J)
0
2
4
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23
Patients
Pain before treatment)
Pain after treatment
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Dr. med. Volkmar Kreisel, Germany: (2015)Neuraxial Low-Level- Laser Therapy for
Lumbar Disc Herniation
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Movie
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Results Middle-age and old-age population
• 8 of 22 middle-age patients (36%) treated with laser therapy achieved 71-80 % painrelief after 6 months
• 9 of 22 middle-age patients (41%) treated with laser therapy achieved 81-90 % painrelief after 6 months
• 2 of 22 middle-age patients (9%) treated with laser therapy achieved 91-100 % painrelief after 6 months
• 3 of 6 old-age patients (50%) treated with laser therapy achieved 71-80 % painrelief after 6 months
• 2 of 6 old-age patients (33%) treated with laser therapy achieved 81-90 % painrelief after 6 months
• 1 of 6 old-age patients (17%) treated with laser therapy achieved 91-100 % painrelief after 6 months
Results Middle-age and old-age population
• 8 of 22 middle-age patients (36%) treated with laser therapy achieved 71-80 % painrelief after 6 months
• 9 of 22 middle-age patients (41%) treated with laser therapy achieved 81-90 % painrelief after 6 months
• 2 of 22 middle-age patients (9%) treated with laser therapy achieved 91-100 % painrelief after 6 months
• 3 of 6 old-age patients (50%) treated with laser therapy achieved 71-80 % painrelief after 6 months
• 2 of 6 old-age patients (33%) treated with laser therapy achieved 81-90 % painrelief after 6 months
• 1 of 6 old-age patients (17%) treated with laser therapy achieved 91-100 % painrelief after 6 months
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The interstial and intraarticular laser therapy
• The laser can be applied in the depth of the tissue close tothe spot of injury
• One or more interstitial needles can be added tosuperficially applied laserneedles
• Pain relief is quicker and more effective
• Combination of metal needle with fiberoptics ( truelaserneedle)
• The laser can be applied in the depth of the tissue close tothe spot of injury
• One or more interstitial needles can be added tosuperficially applied laserneedles
• Pain relief is quicker and more effective
• Combination of metal needle with fiberoptics ( truelaserneedle)
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The interstial and intraarticular laser therapy
• Important in patients with dark skin
• Blue and green laser with anti-inflammatory effects can beapplied as well
• Better effect on tissue regeneration
• Important in patients with dark skin
• Blue and green laser with anti-inflammatory effects can beapplied as well
• Better effect on tissue regeneration
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Combination of laser with platelet richplasma (PRP)
• Serum from patient‘s own blood, enrichedwith cytokines and growth factors
• Injected intraarticulary or interstitially
• Intraarticular and interstitial laserirradiation
• Serum from patient‘s own blood, enrichedwith cytokines and growth factors
• Injected intraarticulary or interstitially
• Intraarticular and interstitial laserirradiation
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Peripheral blood (6% platelets)
6%
94%
0%
PlateletsRed Blood CellsWhite Blood Cells
6%
94%
0%
PlateletsRed Blood CellsWhite Blood Cells
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Preparation of PRP
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Centrifugation of PRP ( low speed)
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PRP in Buffy Coat
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94% Platelets in PRP
94%
5% 1%
PlateletsRed Blood CellsWhite Blood Cells
94%
5% 1%
PlateletsRed Blood CellsWhite Blood Cells
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PRP Composition
1. Plateletsgrowth factors and antiinflammatory cytokines,Interleukin-1 receptor antagonist
2. Neutrophiles40-75% of circulating leukocytes
3. Monocytes2-10% highly motile and migrate to soft tissues
4. Fibroblastsproduce collagen, glycosaminoglycans, glycoproteins
5. KeratinocytesStratified, squamous epithelial cells Primary function is toact as a barrier
6. Small number of primitive stem cells
1. Plateletsgrowth factors and antiinflammatory cytokines,Interleukin-1 receptor antagonist
2. Neutrophiles40-75% of circulating leukocytes
3. Monocytes2-10% highly motile and migrate to soft tissues
4. Fibroblastsproduce collagen, glycosaminoglycans, glycoproteins
5. KeratinocytesStratified, squamous epithelial cells Primary function is toact as a barrier
6. Small number of primitive stem cells
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Chronic inflammation and osteoarthritis
• Interleukin 1 ( Il-1 ) leads to cartilagedamage
• The autologue serum containsincreased amount of Il-1-receptorantagonist ( Il 1 RA)
• Il 1 RA inhibits inflammation andimproves regeneration
• Interleukin 1 ( Il-1 ) leads to cartilagedamage
• The autologue serum containsincreased amount of Il-1-receptorantagonist ( Il 1 RA)
• Il 1 RA inhibits inflammation andimproves regeneration
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Different growth factors in PRP
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Advantages of PRP -Therapy
1. Boosts local healing and tissue (re)growth
2. Natural procedure with patient’s own blood , no side effects ortoxicities
3. Individual therapy
4. Easy handling, procedure doesn’t take longer than 20 min.
5. Supports the body’s own potency of healing6. Cartilage protection and anti-inflammatory effects
7. Prevention or delay of surgery
8. Improvement in quality of life
9. Cost efficiency (no other substances necessary)
10. Can be combined with other methods such as laser therapy
1. Boosts local healing and tissue (re)growth
2. Natural procedure with patient’s own blood , no side effects ortoxicities
3. Individual therapy
4. Easy handling, procedure doesn’t take longer than 20 min.
5. Supports the body’s own potency of healing6. Cartilage protection and anti-inflammatory effects
7. Prevention or delay of surgery
8. Improvement in quality of life
9. Cost efficiency (no other substances necessary)
10. Can be combined with other methods such as laser therapy
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PRP without Laserstimulation
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PRP with Laserstimulation
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STEM CELLSSTEM CELLS•• EmbryonicEmbryonic vsvs Adult Stem CellsAdult Stem Cells
Adult StemCells are moreEthical
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5-10 J/cm2
@ 630-660nm
5-10 J/cm2
@ 630-660nm
APPLICATION OF LILI TO STIMULATE ADSCPROLIFERATION AND VIABILITY
ADDITION OF GROWTH FACTORS TO INDUCEDIFFERENTIATION & APPLICATION
OF LILI TO STIMULATE DIFFERENTATION ANDPROLIFERATION
Adipose derived stem cells and low intensity laserAdipose derived stem cells and low intensity laserirradiationirradiation
APPLICATION OF LILI TO STIMULATE ADSCPROLIFERATION AND VIABILITY
ADDITION OF GROWTH FACTORS TO INDUCEDIFFERENTIATION & APPLICATION
OF LILI TO STIMULATE DIFFERENTATION ANDPROLIFERATION
CARDIACMUSCLE
SKELETALMUSCLE
SMOOTHMUSCLE
SKIN CELLS:KERATINOCYTES
NEURONS
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PHOTO ACTIVATION of stem cells
BioactiveMolecules
Laser irradiation can positively affecthuman stem cells by increasing cellularviability, proliferation and differentiation
CellActivatedCell
BioactiveMolecules
Dormant AwakeLow level Laser Light
light activates cells
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ADIPOSE STEM CELLS (ADSC)
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Stem cells for Osteoarthritis
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Stem cells for Osteoarthritis
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Stem cells for skin rejuvenation
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Stem cells for brain
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Stem cells for brain
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Stem cells for COPD
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Stem cells for diabetes
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Stem cells ED
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Stem cells for heart failure
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Lasers stimulate stem cells for heart repair (Prof. Uri Oron, Tel Aviv)
(WALT-Laserconference, Washington DC, September 2014)
• A simple new process significantly reduces heart scarring afteran ischemic event.
• Discovered by professor Uri Oron at Tel Aviv University, themethod, called shining, consists of applying low-level laserenergy to living bone marrow stem cells a few hours after aheart attack.
• This procedure reduces scarring by up to 80 percent.
• A simple new process significantly reduces heart scarring afteran ischemic event.
• Discovered by professor Uri Oron at Tel Aviv University, themethod, called shining, consists of applying low-level laserenergy to living bone marrow stem cells a few hours after aheart attack.
• This procedure reduces scarring by up to 80 percent.
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Lasers stimulate stem cells for heart repair(Uri Oron, Tel Aviv)
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Lasers stimulate stem cells for kidney repair(Uri Oron, Tel Aviv)
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Lasers stimulate stem cells for kidney repair(Uri Oron, Tel Aviv)
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Fat is a “HighDensity” Sourceof Stem Cells
Fat is a “HighDensity” Sourceof Stem Cells
Tissue/Source of SCs Stem Cell Density
Bone marrow 1 out of 100,000 cellsBone marrow 1 out of 100,000 cellsAdipose tissue 1 out of 100 cellsAdipose tissue 1 out of 100 cells
Tissue/Source of SCs Stem Cell Density
Bone marrow 1 out of 100,000 cellsBone marrow 1 out of 100,000 cellsAdipose tissue 1 out of 100 cellsAdipose tissue 1 out of 100 cells
* In old age
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Comparison of the amount of stem cells in fat andbone marrow (1000:1)
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Adipose derived mesenchymal stem cellls
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Liposuction
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Small incision
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Infiltration cannula
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Tuminescense solution
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Infiltration, 150 – 200 ml
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Liposuction with harvesting cannula
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2 phases, fat on top
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Processing of fat and stem cells
• Indirect sonication
• Centrifugation
• Separation of fat
• Filtration
• Washing
• Freezing (stem cell banking )
• Indirect sonication
• Centrifugation
• Separation of fat
• Filtration
• Washing
• Freezing (stem cell banking )
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Laminar Flow
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Ultrasonic system
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Counting of viable stem cells (Flow cytometry)
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Stem cells can be used for:
- intrarticular injection- interstitial injection- intravenous infusion
followed by laser irradiationwith all spectral colors
Stem cells can be used for:
- intrarticular injection- interstitial injection- intravenous infusion
followed by laser irradiationwith all spectral colors
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Before and 1 year after stem cell therapy
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Before and 1 year after stem cell therapy
Post cell therapy at 12 months improvement of 0.3 mm at posterior condyle
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Ultrasound schock waves for targetingof stem cells
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Evidence-Based Complementary and Alternative MedicineVolume 2013 (2013), Article ID 594906, 12 pages
http://dx.doi.org/10.1155/2013/594906
Research Article
Low-Level Laser Stimulation on Adipose-Tissue-Derived Stem Cell Treatments for Focal Cerebral
Ischemia in Rats
Chiung-Chyi Shen,1,2,3,4 Yi-Chin Yang,1 Ming-Tsang Chiao,1 Shiuh-Chuan Chan,5 and Bai-Shuan Liu6
1Department of Neurosurgery, Taichung Veterans General Hospital,Taichung 40705, Taiwan
Evidence-Based Complementary and Alternative MedicineVolume 2013 (2013), Article ID 594906, 12 pages
http://dx.doi.org/10.1155/2013/594906
Research Article
Low-Level Laser Stimulation on Adipose-Tissue-Derived Stem Cell Treatments for Focal Cerebral
Ischemia in Rats
Chiung-Chyi Shen,1,2,3,4 Yi-Chin Yang,1 Ming-Tsang Chiao,1 Shiuh-Chuan Chan,5 and Bai-Shuan Liu6
1Department of Neurosurgery, Taichung Veterans General Hospital,Taichung 40705, Taiwan
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Differentiation in neuronal cells
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IV Laser Blood IrradiationDr. Michael H. Weber
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IV Lasertreatment with
Red and green laser
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Iv-laser treatment with blue andyellow laser
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Y-needle with 3 luer-lock for iv-Laser withsimultaneous infusion
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Effects of intravenous laser lightirradiation
Red laserStimulation of the immune system, improvement ofblood viscosity
Green laser
Increased oxygen supply
Blue laser:Increased NO, bactericidal effects
Yellow laser:antidepressive effects and more?
All colors stimulate the respiratory chain in themitochondria with increased ATP production.
Red laserStimulation of the immune system, improvement ofblood viscosity
Green laser
Increased oxygen supply
Blue laser:Increased NO, bactericidal effects
Yellow laser:antidepressive effects and more?
All colors stimulate the respiratory chain in themitochondria with increased ATP production.
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Cosmetic laser medicine withMicro- fatgrafting
• Fat preparation by small liposuction
• Separation from tuminscence solution
• Injection of fat below wrinkels for filling(Microfatgrafting)
• Laser stimulation
• Fat preparation by small liposuction
• Separation from tuminscence solution
• Injection of fat below wrinkels for filling(Microfatgrafting)
• Laser stimulation
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Skin aging
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Cosmetic laser medicineNano-Fatgrafting
• Small liposuction
• Separation from tuminesense solution
• Emulsification of the fat
• Injectionen intracutanously with 27 G needle
• Enriched with stem cells
• Laser stimulation
• Small liposuction
• Separation from tuminesense solution
• Emulsification of the fat
• Injectionen intracutanously with 27 G needle
• Enriched with stem cells
• Laser stimulation
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Emulsification process
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Special filter system
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Thank you for your attention