21MALT AGARS

Malt Extract Agar MEH2O 500 mLAgar 10 gMalt 10 g

Malt-yeast Extract MYAH2O 500 mLAgar 10 mLMalt 10 mLYeast 1 mL

Malt Extract with ActivatedCarbon

MEAC

H2O 500 mLAgar 10 gMalt 10 gActivated Carbon 1 g

Malt Extract with Peptone MEPH2O 500 mLAgar 10 gMalt 10 gPeptone 1 g

Malt Extract with Yeast andPeptone

MYAP

H2O 500 mLAgar 10 gMalt 10 gYeast 1 gPeptone 1 g

Malt Extract with Yeast,Peptone, and ActivatedCarbon

MEPYAC

H2O 500 mLAgar 10 gMalt 10 gPeptone 1 gYeast 1 gActivated Carbon 1 g

For the premixed malt agar: Use 25 g

Wheat Malt-yeast Extract WMYAH2O 500 mLAgar 10 mLWheat Malt 10 mLYeast 1 mL

Wheat Malt Extract withPeptone

WMEP

H2O 500 mLAgar 10 gWheat Malt 10 gPeptone 1 g

Wheat Malt Extract withYeast and Peptone

WMYAP

H2O 500 mLAgar 10 gWheat Malt 10 gYeast 1 gPeptone 1 g

Malt Extract with Milo MEMH2O 500 mLAgar 10 gMalt 10 gGround Milo 10 g

Malt Extract with Corn MECH2O 500 mLAgar 10 gMalt 10 gGround Corn 10 g

Malt Extract with Honey MEHH2O 500 mLAgar 10 gMalt 10 gHoney 16 g

ATTC Medium 597 597H2O 500 mLAgar 10 gMalt 3.75 gYeast 0.3 g

Peptone 0.5 g

MYAP with Multi-vitamin MYMPH2O 500 mLAgar 10 gFresh ground crickets 8 gMulti-Vitamin 0.01 gPeptone 1 gYeast 1 gMalt 10 g

MYAP with elements MYPMCZH2O 500 mLAgar 10 gMalt 10 gYeast 1 gPeptone 1 gCalcium 0.01 gMagnesium 0.01 gZinc 0.01 g

Yeast Malt Extract YMEH2O 500 mLPre-mixed Malt Agar 25 gYeast 1 g

Peptone Malt Extract PMEH2O 500 mLPre-mixed Malt Agar 25 gPeptone 1 g

MYA with CS4 CS4MYH2O 500 mLAgar 10 gMalt 3.75 gYeast 0.3 gGround Cordyceps Sinensis 4 10 g

MYA with CSG2 G2MYH2O 500 mLAgar 10 gMalt 3.75 gYeast 0.3 gGround Cordyceps Sinensis G2 10 g

Add Multi-vitamin after sterilization.

Can use just 1 or 2 elements, so labelMYPZ or MYPCZ, etc.

Chitin Method :ch Add 0.3 g chitin to any malt agar

ANIMAL RELATED MEDIA

Blood Worm Agar BWH2O 500 mLAgar 10 gBlood Worms 5 g

Krill Agar KAH2O 500 mLAgar 10 gKrill 10 g

Fish Flake Dextrose Agar FDAH2O 500 mLAgar 10 gFish Flakes 3 gDextrose 10 g

Meal Worm Agar MWH2O 500 mLAgar 10 gMeal Worms (ground) 10 gDextrose 3 gYeast 1 g

Fishy Wax Agar FWAH2O 500 mLAgar 10 gKrill 3.3 gTubiflex Worms 3.3 gFish Flakes 3.3 gBee's Wax 1 g

For 24 test tubes (1 rack) use:432 mL H2O8.6 g Agar8.6 g nutrient0.86 g yeast/peptone

Cat Food Agar CFAH2O 500 mLAgar 10 gCat food 10 g

Dog Food Agar DFAH2O 500 mLAgar 10 gDog food 10 g

Dr. Pollock’s Modified Agar POLLH2O 500 mLAgar 10 gDog food 10 gDextrose or malt extract 2 g

Difco Heart Infusion Agar DHAH2O 500 mLAgar 10 gBeef Heart Infusion from 500g 10Tryptose 5 gNaCl 2.5 g

Difco Charcoal Agar DCAH2O 500 mLAgar 10 gBeef Heart Infusion from 500g 6 gPeptone 5 gNaCl 2.5 gSoluble startch (potato starch) 5 gYeast 1.8 gNorit SG 2 g

Rabbit Food Agar RFAH2O 500 mLAgar 10 gRabbit food 12.5 g

Brown Rice Rabbit Food BRRFH2O 500 mLAgar 10 gBrown Rice flour 10 gPeptone 1 gCrushed Rabbit food 5 g

Boil the rabbit food. Steep for 30minutes. Filter and add agar to filtrate.

Dextrose 1 g

Bird Seed Malt Agar BSMH2O 500 mLAgar 10 gBrown rice Flour 2.5 gGround Bird seed 5 gMalt 2.5 gPeptone 0.5 g

Gold Fish Food Agar GFFAgar 10 gH2O 500 mLGold Fish Food 10 g

GRAIN/STARTCH MEDIA

Milo Agar MAH2O 500 mLAgar 10 gGround milo 10 g

Milo Agar #2 MILOH2O 500 mLAgar 10 gGround milo 20 gYeast 1 g

Modified Sabouraud'sMedium

MSM

H2O 500 mLAgar 10 gBarley flour 25 gYeast 1 gDextrose 5 gNeo-peptone (optional) 2 g

Oatmeal Neo-peptone Agar ONAH2O 500 mLAgar 10 gOatmeal/oat flour 40 gNeo-peptone (optional) 2 g

Cornmeal Dextrose Agar CDAH2O 500 mLAgar 10 gCornmeal 25 gDextrose 3 g

Barley Malt Extract Agar BMAH2O 500 mLAgar 10 gBarley flour 40 gMalt 2 gYeast (optional) 1 g

Teff Flour Malt Extract Agar TFMAH2O 500 mLAgar 10 gTeff flour 40 gMalt 2 gYeast (optional) 1 g

Garbanzo Bean Malt ExtractAgar

GBMA

H2O 500 mLAgar 10 gGarbanzo bean flour 40 gMalt 2 gYeast (optional) 1 g

Rice Bran Malt Extract Agar RBMAH2O 500 mLAgar 10 gRice Bran 40 gMalt 2 gYeast (optional) 1 g

Dark Rice Flour-Malt ExtractAgar

DRMA

H2O 500 mLAgar 10 gDark Rice flour 40 gMalt 2 gYeast (optional) 1 g

Brown Rice flour-Malt ExtractAgar

BRMA

H2O 500 mLAgar 10 gBrown rice flour 40 gMalt 2 gYeast (optional) 1 g

Wheat Flour Malt ExtractAgar

WFMA

H2O 500 mLAgar 10 gwheat flour 40 gMalt 2 gYeast (optional) 1 g

Oat Flour Malt Extract Agar OFMAH2O 500 mLAgar 10 gOat flour 40 gMalt 2 gYeast (optional) 1 g

Green Bean Powder Agar GBPAH2O 500 mLAgar 10 gGreen Bean Powder 10 g

ATCC Medium 336 336H2O 500 mLAgar 10 gDiced Potatoes orPotato Starch

150 g or10 g

Dextrose 10 g

Avocado Peel dextrose Agar APDAH2O 500 mLAgar 10 gAvocado Peels 10 gDextrose 7 g

Potato, Ammonium citrate,Thiamin Agar

PAT

H2O 400 mLAgar 10 gPotato Broth 100 mLKH2PO4 1 gMgSO4 0.5 gPeptone 1.5 gAmmonium Citrate 0.5 gDextrose 15 gThiamine (B1 vitamin) 0.025 g

Potato Dextrose Agar PDAH2O 500 mLAgar 10 gDextrose orHoney/Corn syrup

7 g or10 mL

Diced potatoes 150 g

Potato Dextrose Yeast Agar PDYAH2O 500 mLAgar 10 gDextrose orHoney/Corn syrup

7 g or10 mL

Diced potatoes 150 gYeast 1g

Potato Dextrose Agar PDAH2O 500 mLAgar 10 gDiced and boiled Potatoes orPotato starch

150 g or10 g

Dextrose 7 g

Potato Dextrose Agar PDYH2O 500 mLAgar 10 gDiced and boiled Potatoes orPotato starch

15 g or10 g

Dextrose 7 gYeast 1 g

In the 500 mL of water boil the 150 g ofpotatoes, and use this broth (not thepotatoes). Bring solution back to 500 mLwith water then add remaining ingredients.Potato flakes can be used as a substitute (5g in the 500 mL of water).

Cordyceps militaris known to fruitfrom this and brown rice in jars.

In the 500 mL of water boil the 150 g ofpotatoes, and use this broth (not thepotatoes). Bring solution back to 500 mLwith water then add remaining ingredients.Potato flakes can be used as a substitute (5g in the 500 mL of water).

Potato Skin Dextrose Agar PSDAH2O 500 mLAgar 10 gDried Potato skins 10 gDextrose 7 g

For potato agars made from real potatoes label PP so PPPDA or PPPDY for exampleFor long term storage/ wood decomposers Add 10 g sawdust and label with :SD

Cornmeal Malt Agar CMMAH2O 500 mLAgar 10 gCornmeal 10 gDextrose 7 g

Spore Germination Agar #1 SGA1H2O 500 mLAgar 10 gDifco Cornmeal Agar 8.5 gGlucose (Dextrose) 1 gYeast 0.5 g

Spore Germination Agar #2 SGA2H2O 500 mLDifco Cornmeal Agar 8.5 gGlucose (Dextrose) 3.5 gSucrose 5 gYeast 0.5 gKH2PO4 0.5 g

Spore Germination Agar #3 SGA3H2O 500 mLAgar 10 gCornmeal 10 gMalt 0.8 g

Spore Germination Agar #4 SGA4H2O 500 mLDifco Cornmeal Agar 8.5 gGlucose 1 gSucrose 1.5 gYeast 0.5 g

10 g cornmeal and 10 g agar can besubstituted for the Difco Cornmealagar.

10 g cornmeal and 10 g agar can besubstituted for the Difco Cornmealagar.

10 g cornmeal and 10 g agar can besubstituted for the Difco Cornmealagar.

CHEMICAL MEDIA

Malt with Activated Carbonand KOH

R7

H2O 500 mLAgar 10 gEpsom Salts 0.25 gActivated Carbon 1.3 gMalt 12.5 gHumus 2.5 gKOH 1% 20 mL

Organic Medium Agar OMAH2O 500 mLAgar 10 gGlucose 5 gPeptone 0.5 gYeast 0.05 gKH2PO4 1 gMgSO4x7H2O orMgSO4

0.15 g or0.075 g

Czapek's Medium Czapek'sH2O 500 mLAgar 10 gNaNO3 1.5 gK2HPO4 0.5 gMgSO4 0.25 gKCl 0.25 gFeSO4 orFe SO4x7H2O

0.0187 g or0.0094 g

Dextrose 15 g

JUICE/BROTH MEDIAV8 juice Agar V8AH2O 500 mLAgar 10 gCaCO3 1.5 gV8 Juice 100 mL

Beef Broth Agar BBAH2O 500 mLAgar 10 gBeef Broth 20 mLMalt 5 gYeast 1 g

Chicken Broth Agar CBAH2O 500 mLAgar 10 gChicken Broth 20 mLMalt 5 gYeast 1 g

Butternut Squash Soup Agar BNSAH2O 500 mLAgar 10 gButternut squash soup 20 mLMalt 5 gYeast 1 g

Beef Yeast Agar BYAH2O 300 mLAgar 10 gYeast 2 gBeef Broth 200 mL

MISCELLANEOUS MEDIA

Brine Agar BAH2O 500 mLAgar 10 gBrine 10 g

Sea Water Agar SWAH2O 125 mLAgar 10 g

Dissolve beef extract and peptone byheating H2O. Adjust pH to 7.8, and boilfor 10 minutes. Readjust pH to 7.3 and

Agar 10 gSea Water 375 mLBeef Extract 5 gPeptone 5 gTo Make Artificial Sea Water NaCl 14.065 gKCl 0.385 gCaCl2 0.60 gMgCl2 1.275 gNaHCO3 0.065 g

MgSO4x7H2O 1.75 gH2O 500 mL

Nutrient Agar NAH2O 500 mLPre-mixed NA 12 g

Honey Agar HONH2O 500 mLAgar 10 gHoney 16 g

Nutrient Agar with Honey NAHH2O 500 mLPre-mixed NA 12 gHoney 16 g

Lysogeny Broth LBAgar 10 gH2O 500 mLYeast 2.5 gPeptone 5 gDextrose 10 gOr replace yeast, peptone anddextrose with LB pre-mix

12.5 g

Yeast Peptone Dextrose YPDAgar 10 gH2O 500 mLYeast 5 gPeptone 10 gDextrose 10 g

Yeast Peptone Agar YEPAgar 10 g

add agar.

Also use as broth (no agar).

H2O 500 mLYeast 10 gPeptone 20 gTryptophan 0.32 g

V8 and Honey V8HEMH2O 500 mLAgar 10 gHoney 16 g

V8 Juice 80 mLEgg Shell 0.4 gMilo 1 g

Beet Root Agar BRAH2O 500 mLAgar 10 gBeet Root Supplement 3 gMalt 10 g

Peaches Malt Agar PMAH2O 500 mLAgar 10 gPeaches (canned) 10 gDextrose 7 g

Spirulina Agar SPAH2O 500 mLAgar 10 gSpirulina supplement 3 gMalt 10 g

Nori Agar NorAH2O 500 mLAgar 10 gNori 3 gMalt 10 g

Bean Soup Agar BSAH2O 400 mLAgar 10 gBean Soup 100 mL

Mannitol Egg Yolk PolymyxinAgar

MYP 1) Dissolve agar into water2) Sterilize

AgarH2O 500 mLAgar 10 gPre-mixed MYP 23 gClycloheximide :C 2 gEgg Yolk Solution` 25 mLAntibiotic vial P 4.1 mL

Water Agar H2OH2O 500 mLAgar 10 g

Fish Disease RenibacteriumSalmoninarum Charcoal Agar

FDR

H2O 500 mLAgar 10 gPeptone 5 gYeast 0.3 gL-cysteine hydrochloride 0.5 gActivated Carbon 0.5 g

Variant #1 V1H2O 500 mLAgar 10 gDry sprouted millet 4 gYeast 1 gMalt 10 g

Variant #2 V2H2O 500 mLAgar 10 gDry sprouted oats 4 gYeast 1 gMalt 10 g

Variant #3 V3H2O 500 mLAgar 10 gLive sprouted millet 8 gYeast 1 gMalt 10 g

3) cool to 45-50◦C4) Add egg yolk solution and antibiotic

Adjust pH to 6.8 before autoclaving.

Variant #4 V4H2O 500 mLAgar 10 gLive sprouted oats 8 gYeast 1 gMalt 10 g

Variant #5 V5H2O 500 mLAgar 10 gFresh Ground Corn 12 gYeast 1 gMalt 10 g

For all dry Sprouted grains: place on damp cloth (Scott towel) for about 2 days. Dry for 1 day(completely dry). Grind.For Live sprouted grains: place on damp cloth for about two days. Grind.

For any fresh insect media: Freeze before grinding

Variant #6 V6H2O 500 mLAgar 10 gFresh ground crickets 8 gYeast 1 gMalt 10 g

Variant #7 V7H2O 500 mLAgar 10 gFresh ground crickets 12 gYeast 1 gMalt 10 g

Crickets and Corn CRCH2O 500 mLAgar 10 gFresh ground crickets 8 gFresh Ground Corn 8 gYeast 1 gMalt 10 g

Variant #9 V9H2O 500 mL

Agar 10 gFresh ground crickets 8 gFresh Ground Corn 8 gOyster Shell 0.8 gYeast 1 gMalt 10 g

Variant #10 V10H2O 500 mLAgar 10 g

Fresh ground crickets 8 gFresh Ground Corn 8 gOats 8 gYeast 1 gMalt 10 g

For any fresh insect media: Freeze before grinding

Variant #11 V11H2O 500 mLAgar 10 gFresh Ground Corn 8 gOyster shell 0.8 gYeast 1 gMalt 10 g

Variant #12 (MYA with 2xYeast)

(MYx2A)

H2O 500 mLAgar 10 gYeast 2 gMalt 10 g

Photo-bacterium medium PBMH2O Bring solution up to 500 mLAgar 10 gTris 4 gNH4 0.4 gGlycerol 0.5 gTryptone 5 gYeast 2.5 gSea Water aquarium salt 16.5 gCaCo3 0.5 g

Antibiotic Agar AB

The antibiotic of choice needs to be added toagar after sterilization. in 500mg/500 mL.

Adjust pH to 7.2-7.5 before sterilizing.

H2O 500 mLAgar 10 gLevaquin :L 25 gDoxycycline :D 25 gAmoxicillin :A 25 gSefdinir :CEF 25 gCiprofloxacin :CIP 25 gAzithromyacin :AZI 25 gCephalexin :CLX 25 g

Cycloheximide (Dr. Dan’s Favorite antibiotic)Put 1 mL of 1% cycloheximide solution in through filtered syringe and into any 1L of media (malt andpotato based recommended). To make 1% cycloheximide solution weight out 0.1 g of cycloheximideand bring solution up to 10 mL with water.

Vermiculite Cakes Verm CakesSorghum flour (ground milo) 1 cupH2O 1 cupVermiculite 2 cup

Plug Recipe Plug Recipe50, 000 plugs 1 cupH2O Enough to soak plugsGarbage bag 1Gypsum 4- 8 cupsWheat Bran 6-8 quarts

Media for Liquid Cultures LCH2O 500 mLMalt 10 gPeptone 1 gYeast 0.3 gVegetable oil 5 dropsGround Grain (sorghum, oats,etc)

1 g

1) Grind antibiotic into powder under the hood.2)Add about 10-15 mL pre-sterilized coded H

2O.

3) Mix well.4) When agar has cooled to about 50-60◦C addantibiotic.

Makes 5 cakes.

Soak plugs in water for at least 24 hours.Drain. Mix with gypsum and Wheat brantill each plug is coated. Sterilize inautoclave for 1-2 hours. Inoculate and seal.When mycelium covers plugs drill hole inlog place in plug and seal with wax. Logswill fruit in 6 months to two years.

EXPERIMENTAL AGAR

Carrot Agar CARAgar 10 gH2O 500 mLCarrot mash 20 g

Carrot Malt Agar CMAAgar 10 gH2O 500 mLCarrot mash 20 gMalt 5 g

Complete Media CMAgar 10 gH2O 500 mLSucrose 15 gAmmonium tartrate (NH4-) 2.5 gAmmonium Nitrate (NH4NO3) 0.5 gMagnesium sulfate heptahydrate(MgSO4x7H2O)

0.25 g

Sodium Chloride (NaCl) 0.5 gCalcium chloride (CaCl2) 0.065 gYeast 0.5 gMonopotassium phosphate(KH2PO4)

0.5 g

Sunflower Seed Agar SFSH2O 500 mLAgar 10 gSunflower seeds 10 g

Almond Agar AAH2O 500 mLAgar 10 gAlmonds 10 g

Boil 1 bag of baby carrots in 500 mLwater. Mash carrots. Freeze leftovers.

Boil 1 bag of baby carrots in 500 mLwater. Mash carrots. Freeze leftovers.

MEDIA NOT TO USE!

Cricket Food Agar CAAgar 10 gH2O 500 mLCricket food 20 g

Ovaltine OVAL

H2O 500 mLAgar 10 gOvaltine 10 g

Coffee Raw with Malt Extract C(R)MAH2O 500 mLAgar 10 gMalt 10 gRaw Coffee 10 g

Dark Coffee with Malt Extract C(D)MAH2O 500 mLAgar 10 gDark Coffee Bean 10 gMalt 10 g

Nutrient Agar with Soy NASH2O 500 mLAgar 10 gPre-mixed NA 12 gSoy beans 2 g

Amaranth Soy Agar ASAH2O 500 mLAgar 10 gAmaranth flour 20gSoy flour 20 g

EntheoGenesis #442 EG442H2O 500 mLAgar 10 gMalted Barley 2 g

Reason: failure for mycelium to thrive

Reason: failure for mycelium to thrive

Reason: failure for mycelium to thrive

Reason: Allergies

Reason: Allergies

Reason: Allergies

Reason: failure for mycelium to thrive

Soy flour 10 gPotato Flour 10 gBrown Rice Flour 10 gAmaranth Flour 10 g

Malt Extract with Onion MEOH2O 500 mLAgar 10 gBlended onion 10 gMalt 10 g

Malt Extract with Garlic MEG

H2O 500 mLAgar 10 gBlended Galric 10 gMalt 10 g

Tryptic Soy Agar TSAH2O 500 mLPre-mixed TSA 20 g

Nutrient Agar with Soy NASH2O 500 mLPre-mixed NA 12 gSoybeans 5 g

Peanut Butter Agar PBH2O 500 mLAgar 10 gPeanut Butter 5 g

Coconut Agar CNH2O 500 mLAgar 1 gCoconut milk/juice 10 g

INGREDIENTS TO NEVER USE WITH PRODOCUTION STRAINS

Ingredient ReasonPeanut Butter AllergiesSoy Allergies

Reason: Antifungal

Reason: Antifungal

Reason: Allergies

Reason: Allergies

Reason: Allergies

Reason: Antifungal

Coconut AntifungalDairy/Prebiotics AllergiesEggs AllergiesTree nut (walnuts, pecans, almonds) AllergiesGarlic AntifungalCider Vinegar Failure to thriveOvaltine AntifungalPomegranate AntifungalCinnamon AntifungalCloves AntifungalCurry AntifungalCilantro AntifungalCoffee Failure to thrive