the mechanism of ade s58941418 陳俊良. ade has been proposed as an underlying pathogenic...
Post on 21-Dec-2015
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The mechanism of ADE
S58941418 陳俊良
• ADE has been proposed as an underlying pathogenic mechanism of DHF/ DSS.
• It often occurs in patients with second, heterotypic DENV infections or in infants with maternally transferred dengue immunity.
1. ADE occurs because preexisting subneutralizing antibodies
2. The infecting DENV form complexes that bind to Fc receptor-bearing cells
3. Leading to increased virus uptake and replication
Antibody-dependent enhancement (ADE)
• ADE of DENV-2 infection has also been demonstrated in monkeys infused with a human dengue immune serum.
ND/SK: bind to human and mouse IgGIBN: can’t bind to human and mouse IgG
Antibody-dependent enhancement of infection by murine serum
B. Raji-1 (DC-SIGN) cells:has been shown to facilitate DENVinfection by a mechanism different from ADE
C. The difference in ADE activities among the different DENV serotypes was probably due to sequence variations in the antibody-binding sites.
in vitro IgG 1A5: chimpanzee–human chimeric IgG1 mAbs
in vivo
L-NIL, a specific inhibitor of iNOS
Primary DHF Primary DHF
2nd DHF 2nd DHF