the journal o immun - journal of immunology kahan, ruth berman-goldman, ... iii and gale a. granger...

THE JOURNAL O

ImmUN ®

Official Journal of The American Association of ImmunologistsD[]D

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Published monthly by THE WILLIAMS & WlLKINS COMPANY / Baltimore, Md. 21202 U.S.A. Copyright (D 1976 by The Williams & Wilkins Company / Madeoin the Uni ted States of America.

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THE JOURNAL OF

IMMUNOLOGY

V O L U M E 117

NUMBER 4 TO 6

BALTIMORE, MD. 21202 1976

JOIMA3

COPYRIGHT ~) 1976 aV THE WILLIAMS ~z WILKINS COMPANY

CONTENTS

NUMBER 1, JULY 1976

CELLULAR IMMUNOLOGY

16 Studies on the Immune Response to Fixed Antigens. Preferential Induction of Helper Function with Heavily Trinitrophenylated Sheep Erythrocytes, and Glutaraldehyde-Treated Sheep Erythrocytes. Melvyn Kahan, Ruth Berman-Goldman, Rita Saltoun, and David Naor

40 Generation of Specific Helper Cells and Suppressor Cells in Vitro for the IgE and IgG Antibody Responses. Kimishige Ishizaka and Toshihiro Adachi

48 Enhancement of Early Human E Rosette Formation by Cholinergic Stimuli. Stanley P. Galant, Robert L. Lundak, and Linda Eaton

66 Antibodies to Guinea Pig Lymphokines. II. Suppression of Delayed Hypersensitivity Reactions by a "Second Generation" Goat Antibody against Guinea Pig Lymphokines. Carolyn L. Geczy, Andrew F. Geczy, and Alain L. de Weck

130 Mouse Lymphotoxin. Glennwood Trivers, Dale Braungart, and Edward J. Leonard 151 Studies on the Maturation of Immune Responsiveness in the Mouse. II. Role of the Spleen. Carol A.

Landahl, Ashim Chakravarty, Miriam Sulman, Louis Kubal, and Robert Auerbach 155 Inhibition of Antigen-induced B Lymphocyte Activation in Vitro by Cytochalasin B. Robert L. Roberts,

Jr., and Mariano F. La Via 174 Relationship of CIF, LT, and PIF Released in Vitro by Activated Human Lymphocytes. II. A Further

Functional Comparison of LT and PIF Activities on HeLa and L-929 Target Cells. Edward W. B. Jeffes III and Gale A. Granger

216 Antibody-mediated Basophil Accumulations in Cutaneous Hypersensitivity Reactions of Guinea Pigs. Philip W. Askenase, J. David Haynes, and Betty J. Hayden

274 The Xenogeneic Effect. II. Requirement for Unactivated Murine T Cells during Restoration of Immune Responsiveness with Xenogeneic Reconstitution Factor. John J. Farrar and Janet Fuller-Bonar

283 Regulatory Factors Produced by Lymphocytes. I. The Occurrence of Multiple a-Lymphotoxins Associ- ated with Ribonuclease Activity. Sheng-Chung Lee and Zoltan J. Lucas

313 Induction of Specific Suppressor T Cells in Vitro. Diane D. Eardley and Richard K. Gershon 319 Suppressor Cells in Spleens from "Nude" Mice: Their Effect on the Mitogenic Response of B

Lymphocytes. J. Bruce Smith and Gordon J. Eaton 323 Biological Expressions of Lymphocyte Activation. V. Characterization of a Soluble Immune Response

Suppressor (SIRS) Produced by Concanavalin A-Activated Spleen Cells. Takushi Tadakuma, Alice L. K~ihner, Robert R. Rich, John R. David, and Carl W. Pierce

331 Interaction of Soluble C3 Fragments with Guinea Pig Lymphocytes. Comparison of Effects of C3a, C3b, C3c, and C3d on Lymphokine Production and Lymphocyte Proliferation. W.J. Koopman, A. L. Sandberg, S. M. Wahl, and S. E. Mergenhagen

337 Divergent Effects of Cyclophosphamide Administration on Mononuclear Killer Cells: Quantitative Depletion of Cell Numbers versus Qualitative Suppression of Functional Capabilities. Gary W. Hunninghake and Anthony S. Fauci

CLINICAL IMMUNOLOGY

84 Measurement of IgG-Blocking Antibodies: Development and Application of a Radioimmunoassay. Anne K. Sobotka, Martin D. Valentine, Kimishige Ishizaka, and Lawrence M. Lichtenstein

97 In Vitro Production of IgE by Human Peripheral Blood Lymphocytes: Effect of Cholera Toxin and // Adrenergic Stimulation. Roy Patterson, Irena M. Suszko, W. James Metzger, and Mary Roberts

115 Identification of T and B Cell Subpopulations in Human Peripheral Blood: Electropheretic Mobility Distributions Associated with Surface Marker Characteristics. Joel H. Kaplan and E. E. Uzgiris

246 Chemotactic Responses of Normal Human Basophils to C5a and to Lymphocyte-derived Chemotactic Factor. Michael A. Lett-Brown, David A. Boetcher, and Edward J. Leonard

IMMUNOCHEMISTRY

7 Structural Studies on Induced Antibodies with Defined Idiotypic Specificities. III. N-Terminal Amino Acid Sequence of the Heavy and Light Chains of Mouse Anti-Streptococcal Antibodies--A5A, $8, and Sl17. J. Donald Capra, Claudia Berek, and Klaus Eichmann

79 The Ultrastructure of Clt, a Subcomponent of the First Component of Complement: An E. M. and Ultracentrifuge Study. L. Pinteric, S. N. Assimeh, D. I. C. KeUs, and R. H. Painter

160 Diversity among Rabbit Antibody Light Chain Amino Terminal Sequences. L. Edward Cannon, Michael N. Margolies, A. Donny Strosberg, Francis W. Chen, John NeweU, and Edgar Haber

180 Fine Structure of Three Different Anti-fiuorescein-Combining Sites: Induced Circular Dichroism of Hapten Bound to Autologous and Heterologous Recombinants. John R. GoUogly and Renata E. Cathou

iii

iv CONTENTS [VOL. 117

259 Analysis of Immunoglobulin Genes: DNA/RNA Hybridization with Immunoglobulin K-Chain mRNA and Isolation and Translation of Hybridized RNA. Ursula Storb and Stephanie Marvin

269 Characterization of an Antiserum Specific for Cell Surface Antigens of Rat Mast Cells. Suk Hing Yiu and Arnold Froese

298 Visualization of Type I and II Collagens in Tissue Sections by Immunohistologic Techniques. Georg Wick, Hans Nowack, Eckhart Hahn, Rupert Timpl, and Edward J. Miller

304 Clq (C1) Receptor on Human Platelets: Inhibition of Collagen-Induced Platelet Aggregation by Clq (CI) Molecules. Eva A. Suba and Gyorgy Csako

310 Studies on Glycopeptide Released by Trypsin from Sheep Erythrocytes. Takeshi Kitao, Masazumi Takeshita, and Kenichi Hattori

IMMUNOGENETICS AND TRANSPLANTATION

33 Isolation and Partial Characterization of AgF-I-A Rat Lymphocyte Membrane Antigen. PhiUip B. C. Williams and Charles W. DeWitt

91 Identification of a Butanol-Extractable Human Placenta-Specific Antigen with Alkaline Phosphatase Activity. Chin-Hai Chang and Dennis AngeUis

124 Allotype Suppression with Anti-VH Allotype Antibody: Concurrent Suppression of VH and GH Allotypes among IgG, IgM, and IgA Molecules in Multi-heterozygous Rabbits. Daniel P. Eskinazi, Alice Gilman-Sachs, Katherine L. Knight, and Sheldon Dray

136 An Improved Method for Isolation of H-2 and Ia Alloantigens with Immunoprecipitation Induced by Protein A-Bearing Staphylococci. Susan E. Cullen and Benjamin D. Schwartz

143 Effect of Six Weekly Transfusions on Canine Marrow Grafts: Tests for Sensitization and Abrogation of Sensitization by Procarbazine and Anti-thymocyte Serum. Paul L. Weiden, Rainer Storb, Sherrill Slichter, Reed P. Warren, and George E. Sale

242 Blocking of MLC Stimulation by Anti-Ia Sera: Studies with the Virus Plaque Assay. Shogo Kano, Barry R. Bloom, and Donald C. Shreffler

343 Hybridization Kinetics of Ribonucleic Acid and Deoxyribonucleic Acid from Antigen-Stimulated Mouse Spleen Cells. Neomi Moav, Drora Goldblatt, Boaz Moav, and Asher Frensdorf[

IMMUNOPATHOLOGY

1 Interaction between Lymphocytes and Inflammatory Exudate Cells. II. A Proteolytic Enzyme Released by PMN as a Possible Mediator for Enhancement of Thymocyte Response. Shuji Nakamura, Masaru Yoshinaga, and Hideo Hayashi

27 The Requirement for Esterase Activation in the Anti-immunoglobulin-Triggered Movement of B Lymphocytes. Elmer L. Becket and Emil R. Unanue

110 Protection against Experimental Allergic Encephalomyelitis with Peptides Derived from Myelin Basic Protein: Presence of Intact Encephalitogenic Site is Essential. Bernard F. Driscoll, Marian W. Kies, and EUsworth C. Alvord, Jr.

187 Inflammatory Process in Murine Lymphocytic Choriomeningitis Is Maximal in H-2K or H-2D Compati- ble Interactions. Peter C. Doherty, Malcolm B. C. Dunlop, Christopher R. Parish, and Roll M. Zinkernagel

225 Immunosuppression of Experimental Autoimmune Myasthenia Gravis by Hydrocortisone and Azathio- prine. Oded Abramsky, Rebeca Tarrab-Hazdai, Aharon Aharonov, and Sara Fuchs

229 Eosinophil Chemotactic Factor (ECF). I. Release from Polymorphonuclear Leukocytes by the Calcium Ionophore A23187. Beate M. Czarnetzki, Wolfgang Ki~nig, and Lawrence M. Lichtenstein

235 Eosinophil Chemotactie Factor (ECF). II. Release from Human Polymorphonuelear Leukecytes during Phagocytosis. Wolfgang K6nig, Beate M. Czarnetzki, and Lawrence'M. Lichtenstein

TUMOR IMMUNOLOGY

11 Immunologic Mechanisms in the Pathogenesis of Virus-Induced Murine Leukemia. Ill. Target Cell Specificity of Autoreactive Thyrnocytes. Max R. Proffitt, Martin S. Hirsch, David A. Ellis, Beatriz Gheridhn, and Paul H. Black

52 Characterization of Effector Lymphocytes Associated with Immunity to Murine Sarcoma Virus (MSV)-Induced Tumors. I. Physical Properties of Cytolytic T Lymphocytes Generated in Vitro and of Their Immediate Progenitors. Fernando Plata, H. Robson MacDonald, and Howard D. Engers

106 Studies on the Terminal Stages of Antibody-Complement-Mediated Killing of a Tumor Cell. III. Effect of Membrane Active Agents. Michael D. P. Boyle, Sarkis H. Ohanian, and Tibor Borsos

292 Separation of Populations of Sensitized Lymphoid Coils into Fractions Inhibiting and Fractions Enhancing Syngeneic Tumor Growth in Vivo. Myra Small and Nathan Trainin

VIRAL AND MICROBIAL IMMUNOLOGY

23 Detection of Antibodies to Bacterial Cell Wall Peptidoglycan in Human Sera. Berno Heymer, Karl-Heinz Schlei[er, Stanley Read, John B. Zabriskie, and Richard M. Krause

59 The Course of Schistosoma mansoni Infection in Thymectomized Rats. Donato Cioli and Gunther Dennert 73 BasophiI-Sensitizing Antibody Response to Herpes Simplex Viruses in Rabbits. Richard P. Day, John

Bienenstock, and William E. Rawls

1976l CONTENTS

102 Association of Dane Part ic les with e Antigen in the Serum of Asymptomatic Carr iers of Hepati t is B Surface Antigen. Kazuaki Takahashi, Mitsunobu Imai, Fumio Tsuda, Takashi Takahashi, Yuzo Miyakawa, and Makoto Mayumi

168 Antigenic Analysis of the Major St ructura l Protein of the Mason-Pfizer Monkey Virus. G. Schochet- man, M. Boehm-Truitt, and J. Schlom

191 Humoral Immuni ty in Experimental Syphilis. I. The Demonstrat ion of Resistance Conferred by Pass ive Immunizat ion. Nancy H. Bishop and James N. Miller

197 Humoral Immuni ty in Experimental Syphilis. I I . The Relationship of Neutral izing Factors in Immune Serum to Acquired Resistance. Nancy H. Bishop and James N. Miller

208 Analysis of Complement-Dependent Antibody-Modiatod Lysis of Targe t Cells Acutely Infected with Measles. John T. Hicks, Michael J. Klutch, Paul Albrecht, and Michael M. Frank

253 Appearance of IgG (Fc) Receptor(s) on Cultured Human Fibroblasts Infected with Human Cytomegalo- virus. Adel A. Rahman, Marion Teschner, Krishan K. Sethi, and Henning Brandis

v

COMMUNICATIONS

350 Antibody to Murine Leukemia Virus: Genetic Control Linked to the H-2 Locus in PL Mice. Robert C. Nowinski and Theresa Doyle

352 LETTERS "TO THE EDITOR

355 ANNOUNCEMENTS

355 ERRATUM

356 AUTHOR INDEX

NUMBER 2, AUGUST 1976

CELLULAR IMMUNOLOGY

381 Induction of Resis tance to Toxoplasma Gondii in Human Macrophages by Soluble Lymphocyte Products. Seth E. Anderson, Simeon Bautista, and Jack S. Remington.

388 Function of 2-Mercaptoethanol as a Macrophage Substitute in the P r imary Immune Response in Vitro. Hilmar Lemke and Hans-Georg Opitz

396 Regulation of Antibody Response in Different Immunoglobulin Classes. I. Selective Suppression of Anti-DNP IgE Antibody Response by Preadminis t ra t ion of DNP-Coupled Mycobacter ium. Tada- mitsu Kishimoto, Yoshikatsu Hirai, Masaki Suemura, and Yuichi Yamamura

419 lmmunogen Structure and Cell Selection. I. Cellular Requirements for the In Vitro Response to Moderately Haptenated Forms of the Solid-Phase Immunogen DNP-O-Bio-GeI-P. Gary N. Trump

423 Regulation of the Immune System by Synthetic Polynucleotides. VI I . Amplification of the Immune Response in Young and Aging Mice. Ihn H. Han and Arthur G. Johnson

463 B Lymphocytes in the Guinea Pig Thymus are Specifically Localized in the Central Area. Jan P. A. Baak, Chris J. L. M. Meijer, Arie C. Nieuwenhuyzen Kruseman, Henk L. Langevoort, and Louis Kater

467 Structure and Biologic Functions of Human IgD. X. Enhancement of P H A Responsiveness by Anti-5-Activated Lymphocytes. Vail Kermani-Arab, Denis R. Burger, and Gerrie A. Leslie

480 Requirements for Induction of T Cell Tolerance to DNFB: Efficiency of Membrane-Associated DNFB. Henry N. Claman and Stephen D. Miller

504 Generation of Cytologic T Lymphocytes in Vitro. VIII . Fai lure of Anti-RS Antisera to Inhibit the Generation of Cytolytic T Lymphocytes or Cytolytic T Lymphocyte Activity. Frank W. Fitch and Hansruedy Ramseier

548 Lymphokine-Like Factors Produced by Human Lymphoid Cell Lines with B or T Cell Surface Markers . Takeshi Yoshida, Tadatoshi Kuratsuji, Akikazu Takada, Yumiko Takada, Jun Minowada, and Stanley Cohen

608 Recrui tment of Effector Lymphocytes by Ini t ia tor Lymphocytes: Circulating Lymphocytes are Trapped in the Reacting Lymph Nodes. Shmuel Livnat and Irun R. Cohen

614 Recruitment of Effector Lymphocytes by Ini t ia tor Lymphocytes: Recruited Lymphocytes are Im- munospecific and Include Graft-versus-Host-Reactive Lymphocytes. Shmuel Livnat and Irun R. Cohen

666 In Vitro Activation of Mouse Macrophages by Rat Lymphocyte Mediators. Isaiah J. Fidler, John H. Darnell, and Marilyn B. Budmen

690 Radioimmunoassay for Thymopoietin. Gideon Goldstein

428

CLINICAL IMMUNOLOGY

In Vitro Stimulation of Neutrophil Motility by Levamisole. Maintenance of cGMP Levels in Chemo- tactically Stimulated Levamisole-Treated Neutrophils. Ronald Anderson, Annemarie Glover, Hendrik J. Koornhof, and Arthur R. Rabson

vi CONTENTS [VOL. 117

IMMUNOCHEMISTRY

364 Isolation and Prel iminary Characterization of Two Varieties of Low Molecular Weight Immunoglo- bulin in the Bullfrog, Rana Catesbeiana. Christopher Green and Lisa A. St~iner

375 Low Molecular Weight Immunoglobulins in Rana Catesbeiana Tadpoles. Christopher Green and Lisa A. Steiner

405 Purification of Native Properdin by Reversed Affinity Chromatography and its Activation by Pro- teolytic Enzymes. Joe O. Minta

416 Cross-Reactivity of Human /32-Microglobulin with Human Granulocyte Colony-Stimulating Activity. G. B. Price, E. A. McCulloch, and J. E. Till

447 Cross-Reactive T Cell Antigens among Mammal ian Species. C. M. Balch, M. K. Dagg, and M. D. Cooper 457 Receptor-Blocking Factor Present in Immune Serum Resembling Auto-Anti-Idiotype Antibody. Richard

B. Bankert and David Pressman 486 Cold Activation of Complement. I. Presence of Coagulation-Related Activator. Motoharu Kondo,

Keimei Hosokawa, Masasuke Masuda 497 Rat T Lymphocyte-Specific Antigens and Their Cross-Reactivity with Mouse T Cells. Yoshifumi Ishii,

Hiro[uni Koshiba, Hiroshi Yamaoka, and Kokichi Kikuchi 511 Isolation of a Murine Leukemia Fc Receptor by Selective Release Induced by Surface Redistribu-

tion. Sheldon M. Cooper and Yugalkishore Sambray 518 Par t ia l Regain of Activity in Heterologous Recombinants of Phosphorylcholine-binding M-compo-

nents from Different Species. W.F. Riesen 569 Analysis of the Diversity of Murine Antibodies to Dextran B1355. I. Generation of a Large, Pauci-

clonal Response by a Bacterial Vaccine. Daniel Hansburg, David E. Briles, and Joseph M. Davie 587 Immunoglobulin Binding by Mouse Intestinal Epithelial Cell Receptors, Ruth L. Guyer, Marian E.

Koshland, and Paul M. Knop[ 630 C3 Requirements for Formation of Alternative Pa thway C5 Convertase. Mohamed R. Daha, Douglas

T. Fearon, and K. Frank Austen 639 Demonstration of Binding Sites for IgG Fc and the Third Complement Component (C3) on Isolated

Hepatocytes. Uwe Hopf, Karl-Hermann Meyer zum Buschenfelde, and Manfred P. Dierich 661 The Rat Basophilic Leukemia Cell Receptor for IgE. I. Characterization as a Glycoprotein.

Anthony Kulczycki, Jr., Ter O, A. McNearney, and Charles W. Parker


413 Measurement of H-2 and Non-H-2 Antigens in the Mouse with the Footpad Swelling Test. Neal D. Kon and Paul A. Klein

477 Studies on the Genetic Control of Antibody Affinity: The Independent Control of Antibody Levels and Affinity in Biozzi Mice. Fay E. Katz and Michael W. Steward

491 Lack of Association of Human "B Cell" Antigens and Fc Receptor Activity of Antibody-Dependent Cytotoxic Lymphocytes. June L. Miller, Robert E. Humphreys, and Dean L. Mann

531 T Lymphocyte-Enriched Murine Peritoneal Exudate Cells. I I I . Inhibition of Antigen-Induced T Lympho- cyte Proliferation with Anti-Ia Antisera. Ronald H. Schwartz, CheUa S. David, David H. Sachs, and William E. Paul

555 Specificity of Allogeneic and Xenogeneie Cell Recognition in the Fetal Lamb. Tuula Asantila and Paavo Toivanen

626 Localized Deletion-Insertion Mutations: A Major Factor in the Evolution of Immunoglobulin Struc- tural Variability. Michael Potter, Eduardo Padlan, and Stuart Rudikoff

679 A Homologue of the Rabbit Kappa Chain Allotype b9 on IgG from a Cottontail Rabbit. Simonetta Landucci Tosi, Rose G. Mage, and Roberto M. Tosi

686 The Homologues of Rabbit b-Allotypes on Hare Immunoglobulin Light Chains: An Evolutionary Puzzle. Simonetta Landucci Tosi, Rose G. Mage, and Roberto M. Tosi

IMMUNOPATHOLOGY

433 The Inhibitory Effect of Chemotactic Factors on Erythrophagocytosis by Human Neutrophils. Robert A. Musson and Elmer L. Becker

450 Complement-Mediated Release of Histamine from Human Basophils. II. Biochemical Characteriza- tion of the Reaction. J. Andrew Grant, Linda Settle, Elbert B. Whorton, and Elton Dupree

471 Duodenal Immunoglobulin Deficiency in Graft-versus-Host Disease (GVHD) Mice. Jerome A. Gold, Jon Kosek, Nancy Wanek, and Sonia Baur

523 Mechanisms Involved in Elimination of Organisms from Experimental Cutaneous Candida albicans Infections in Guinea Pigs. Peter G. Sohnle, Michael M. Frank, and Charles H. Kirkpatrick

576 T Cell Requirement for Experimental Allergic Encephalomyelitis Induction in the Rat. Librado Ortiz-Ortiz, Robert M. Nakamura, and William O. Weigte

594 Histamine Release by Chemotactic, Formyl Methionine-Containing Peptides. William A. Hook, Elliott Schiffmann, Srinivasabhat Aswanikumar, and Reuben P. Siraganian

674 Biologic Properties of Nontoxic Derivatives of A Lipopolysaecharide from E. coil K235. Floyd C. Mclntire, Martha P. Hargie, J. R. Schenck, Richard A. Finley, H. W. Sievert, Ernst Th. Rietschel, and David L. Roser~treich

1976 ] CONTENTS vii

TUMOR IMMUNOLOGY

440 Two Functionally Distinct Anti-tumor Effector Cells Isolated from Pr imary Murine Sarcoma Virus- Induced Tumors. Howard T. Holden, J. Stephen Haskill, Holger Kirchner, and Ronald B. Herberman

541 Murine P lasma Cells Secreting More than One Class of Immunoglobulin Heavy-Chain. I. Frequency of Two or More M-components in Ascitic Fluids from 788 P r imary Plasmacytomas. Herbert C. Morse, III, Janet G. Pumphrey, Michael Potter, and Richard Asofsky

563 Cellular Specificity of Plasmacytoma-Induced Immunosuppression. Susan ZoUa-Pazner, Bessie Sulli- van, and Deborah Richardson

580 Control of Fc and C3 Receptors on Myeloid Leukemic Cells. Joseph Lotem and Leo Sachs 597 Enzymatic Degradat ion of Tumor Cells Damaged by Antibody Plus Complement. Arnold E. Reif and

Cynthia M. Robinson 620 Strain-, Age-, and Tumor-Dependent Distribution of Terminal Deoxynuclectidyl Transferase in Thy-

mocytes of Mice. Nelson H. Pazmino and James N. Ihle 646 Altered Lymphocyte Functions in Rats Bearing Syngeneic Moloney Sarcoma Tumors. I. Mitogen

Responses, Mixed Lymphocyte Reactions (MLR), and Mixed Lymphocyte-Tumor Reactions (MLTR). Bruce C. Veit and Joseph D. Feldman

655 Altered Lymphocyte Functions in Rats Bearing Syngeneic Moloney Sarcoma Tumors. I I . Suppressor Cells. Bruce C. Veit and Joseph D. Feldman

697 Suppression of Allogeneic Lymphomas in Spleens of I r radiated Mice. Importance of the D end of the H-2 Complex. Enzo Bonmassar and Gustavo Cudkowicz


357 Cellular Immuni ty in Chronic Theiler 's Virus Central Nervous System Infection. Stanley G. Rabino- witz and Howard L. Lipton

602 Inhibition by Rheumatoid Factor, Anti-Fc, and Staphylococcal Protein A of Antibody-Dependent Cell- Mediated Cytolysis against Herpes simplex Virus-Infected Cells. R. Marshall Austin and Charles A. Daniels

635 Neonatal Infection with Mouse Thymic Virus: Differential Effects on T Cells Mediating the Graft- versus-Host Reaction. Sue S. Cross, Herbert C. Morse, III, and Richard Asofsky

693 Immune Response of the Mouse to the Major Core Protein (p30) of Ecotropic Leukemia Viruses. Robert C. Nowinski and Andrew Watson

COMMUNICATIONS

701 Deficient Production of a Thymus-Dependent High Affinity Antibody Subset in Mice (CBA/N) with an X-Linked B Lymphocyte Defect. Richard K. Gershon and Kazunari Kondo

703 In Vivo Activation of Properdin Factor B in Normotensive Bacteremic Individuals. Alan G. Palestine and Martin R. Klemperer

705 Soluble Antigens and Antibodies in Sera from Baboons Infected with Schistosoma mansoni. Vaclav Houba, Davy K. Koech, Robert F. Sturrock, Anthony E. Butterworth, John R. Kusel, and Adel A. F. Mahmoud

708 Genetic Control of the Immune Response in Guinea Pigs to the Known Sequence Polymer (Tyr-Ala- Glu-Gly)n. Allen R. Zeiger and Paul H. Maurer

711 Announcements 711 Addendum 712 Author Index

NUMBER 3, SEPTEMBER 1976

CELLULAR IMMUNOLOGY

741 Cutaneous Basophil Hypersensit ivi ty Uncovered in the Cell Transfer of Classical Tuberculin Hyper- sensitivity. Philip W. Askenase

802 Tolerance and Contact Sensitivity to DNFB in Mice. VI. Inhibition of Afferent Sensitivity by Sup- pressor T Cells in Adoptive Tolerance. John W. Moorhead

818 Studies on Human Brain-Thymus Cross-Reactive Antigens. T.P. Maznina and S. G. Kushner 835 Immune Response in the Hamster . VII I . Ig Class Differences in Susceptibility to Tolerance Indue-

tion. J. L. Portis and J. E. Coe 865 Leukocyte Migration Inhibitory Factor (LMIF) Production in Unidirectional Mixed Lymphocyte

Cultures. Andrzej J. G6rski, Bo Dupont, John A. Hansen, and Robert A. Good 962 Circadian Rhythmic Plaque-Forming Cell Response of Spleens from Mice Immunized with SRBC.

Gabriel Fernandes, Franz Halberg, Edmond J. Yunis, and Robert A. Good 967 Site of Action of a Soluble Immune Response Suppressor (SIRS) Produced by Concanavalin A-

Activated Spleen Cells. Takushi Tadakuma and Carl W. Pierce 1045 The Kinetics of Interferon Production by Mouse Lymphocytes and I ts Modulating Effect on the Virus

Plaque-Forming Cell Assay as a Quanti tat ive Method to Determine Activated Lymphocytes. Jochen W. Heine and William H. Adler

° . °

VlU CONTENTS [VOL. 117

CLINICAL IMMUNOLOGY

717 A Human Cold Agglutinin which Binds Lacto-N-neotetraose. Chao-Ming Tsai, David A. Zopf, Richard Wistar, Jr., and Victor Ginsburg

730 The Formation of Stable E-Rosettes by Human T Lymphocytes Activated in Mixed Lymphocyte Reactions. Uri Galili and Michael Schlesinger

782 Human Transfer Factor: Effects on Lymphocyte Transformation. Denis R. Burger, Arthur A. Vanden- bark, Patricia Finke, James E. Nolte, and R. Mark Vetto

789 Human Transfer Factor: Fractignation and Biologic Activity. Denis R. Burger, Arthur A. Vanden- bark, Doyle Dares, William A. Anderson, Jr., R. Mark Vetto, and Patricia Finke

797 Nicotinamide: Suppression of Lymphocyte Transformation with a Component Identified in Human Transfer Factor. Denis R. Burger, Arthur A. Vandenbark, Doyle Dares, William A. Anderson, Jr., R. Mark Vetto, and Patricia Finke

927 Suppression of Reaginic Antibody Formation. IV. Suppression of Reaginic Antibodies to Penicillin in the Mouse. Weng Y. Lee and Alec H. Sehon

939 An Appraisal of Fc Receptors on Human Peripheral Blood B and L Lymphocytes. Peter I. Lobo and David A. Horwitz

944 Development of Monoclonal IgA and an Apparent IgG in a Patient with Macroglobulinemia: Sharing of Individually Specific Antigenic Determinants Among IgM, IgA, and IgG. Daryl S. Fair, Susan Schaffer, and Robert G. Krueger

IMMUNOCHEMISTRY

774 Interaction of C-Reactive Protein with Lymphocytes and Monocytes. Complement-Dependent Ad- herence and Phagocytosis. Richard F. Mortensen, Alexander P. Osmand, Thomas F. Lint, and Henry Gewurz

807 Purification, Specificity, and Hypervariable Region Sequence of Anti-pneumococcal Polysaccharide Antibodies Elicited in a Single Rabbit. Francis W. Chen, L. Edward Cannon, Michael N. Margolies, A. Donny Strasberg, and Edgar Haber

830 A New Activity of Complement Component C3: Cell-Bound C3b Potentiates Lysis of Erythrocytes by C5b, 6 and Terminal Components. Carl H. Hammer, Aaron S. Abramovitz, and Manfred M. Mayer

841 C4 Synthesis in C4-Deficient Guinea Pig Radiation Chimeras: Restoration of the Classic Complement Pathway. Robert O. Webster, Ulrich H. Rudofsky, and Richard J. Pickering

847 Synthetic Glycelipid Adjuvants. Ulrich H. Behling, Barbara Campbell, Chung-Ming Chang, Christopher Rumpf, and Alois Nowotny

882 Structure and Function of Immunoglobulin Domains. V. Binding of Immunoglobulin G and Fragments to Placental Membrane Preparations. Trudy McNabb, T. Y. Koh, K. J. Dorrington, and R. H. Painter

900 Evidence for the Presence of Components of the Alternative (Properdin) Pathway of Complement Activation in Respiratory Secretions. J. Robertson, J. R. Caldwell, J. R. Castle, and R. H. Waldman

986 Studies of Idiotypic Antibodies: Reactions of Isologous and Autologous Anti-idiotypic Antibodies with the Same Antibody Preparations. L. Scott Rodkey

990 The Primary Structure of Porcine C3a Anaphylatoxin. Neal C. Corbin and Tony E. Hugli 1028 Intestinal Uptake of Macromolecules. V. Comparison of the in Vitro Uptake by Rat Small Intestine

of Antigen-Antibody Complexes Prepared in Antibody or Antigen Excess. W. Allan Walker, Stephen N. Abel, Margaret Wu, and Kurt J. Bloch


736 Ability of H-2 Regions to Induce Graft-vs-Host Disease. Jan Klein and ChinLee Chiang 754 In Vitro Induction of F~ Hybrid Anti-parent Cell-Mediated Cytotoxicity. Gene M. Shearer, Carol A.

Garbarino, and Gustavo Cudkowicz 765 HLA-Related Control of Spontaneous and Antibody-Dependent Cell-Mediated Cytotoxic Activity in

Humans. Daniela Santoli, Giorgio Trinchieri, Chester M. Zmijewski, and Hilary Koprowski 771 Influence of H-2 on the Ontogenesis of a Spleen Cell Population which Lacks C3 Receptors and Thy-1

Antigen. Arturo Ferreira and Victor Nussenzweig 814 Immune Response of Mice to the Thy-l.1 Antigen: Intra-H-2 Mapping of the Complementary Ir-Thy-1

Loci. Marek B. Zaleski and Jan Klein 871 Participation of Cyclophosphamide-Sensitive T Cells in Graft-vs-Host Reactions. Anthony Schwartz,

Simone Orbach-Arbouys, and Richard K. Gershon 981 Studies on the Specificity of in Vitro Induced Lymphocytotoxicity to SV40-Transformed Fibroblasts.

H. Warnatz and F. Krapf

713

IMMUNOPATHO LO GY

Modulation of Cyclic AMP in Purified Rat Mast Cells. III. Studies on the Effects of Concanavalin A and Anti-IgE on Cyclic AMP Concentrations during Histamine Release. Timothy J. Sullivan, Keith L. Parker, Anthony Kulczycki, Jr., and Charles W. Parker

1976] CONTENTS

760 Thymic Function in NZB Mice. II. Regulatory Influence of a Circulating Thymic Factor on Anti- body Production against Polyvinylpyrrolidone in NZB Mice. Marie-Anne Bach and Patrick Niaudet

935 The Complement Profile in Babesiosis. William E. Chapman and Peter A. Ward 973 Humoral Immunostimulation. VI. Increased Calcium Uptake by Coils Treated with Antibody and

Complement. William T. Shearer, John P. Atkinson, and Charles W. Parker 1010 Bence Jones Proteins and Light Chains of Immunoglobulins. XIII. Effects of Elastase-like and Chymo-

trypsin-like Neutral Proteases Derived from Human Granulocytes on Bence Jones Proteins. Alan Solomon, Wilhelm Schmidt, and Klaus Havemann

1015 Colchicine-Sensitive Structures~ and Lymphocyte Activation. Warner C. Greene, Christine M. Parker, and Charles W. Parker

1039 Separation of Slow Reacting Substance of Anaphylaxis (SRS-A) from Human Lung into Four Biologi- cally Active Fractions. Hidenobu Takahashi, Marion E. Webster, and Harold H. NewbaU

1049 Histamine Release from Cultured Human Basophils: Lack of Histamine Resynthesis After Antigenic Release. Jeffrey D. Drobis and Reuben P. Siraganian

ix

TUMOR IMMUNOLOGY

822 Antibody-Induced Killing in Vivo of L1210/MTX-R Cells Quantitated in Passively Immunized Mice with ~s~I-Iododeoxyuridine-Labeled Coils and Whole Body Measurement of Retained Radioactivity. George A. Carlson and Geronimo Terres

889 Immunosuppression by Spleen Cells from Moloney Leukemia. II. Studies on the Mechanism of Suppres- sion and Failure to Detect an Extracellular Suppressive Product. Ronald A. Stiller and Jan Cerny

904 An in Vitro Immune, Enzymatic Assay of Tumor Antigens in the Mouse With ~-Galactesidase. Franco Ce/ada, Pier Giorgio Natali, and Jasna Radojkovic

911 An Indirect Radioimmunoassay for Thymus Leukemia (TL) Antigens. Naomi L. Esmon and J. Russell Little

919 Different Mechanisms for the Modulation of TL Antigens on Murine Lymphoid Cells. Naomi L. Esmon and J. Russell Little

1023 Early Steps in Specific Tumor Cell Lysis by Sensitized Mouse T Lymphocytes. II. Electrolyte Permea- bility Increase in the Target Cell Membrane Concomitant with Programming for Lysis. Eric Martz

1033 Inhibition of Normal Mouse Lymphocyte Mitegen Responses by Xenogeneic or Allogeneic Antibodies to the MuLV Glycoprotein gp71. John C. Lee and James N. lhle


722 Immunochemical Studies of Infectious Mononucleosis. V. Isolation and Characterization of a Glyco- protein from Goat Erythrocyte Membranes. Mary Ann Fletcher, Timothy M. Lo, and William R. Graves

748 The Role of Circulating Interferon in the Modifications of Immune Responsiveness by Mouse Hepatitis Virus (MHV-3). Jean-Louis Virelizier, Anne-Marie Virelizier, and Anthony C. Allison

852 Influenza A Virus Interaction with Murine Lymphocytes. I. The Influence of Influenza Virus A/Japan 306 (H2N2) on the Pattern of Migration of Recirculating Lymphocytes. Judith J. Woodruff and Jack F. Woodruff

859 Influenza A Virus Interaction with Murine Lymphocytes. II. Changes in Lymphocyte Surface Proper- ties Induced by Influenza Virus A/Japan 305 (H2N2). Judith J. Woodruff and Jack F. Woodruff

876 Hepatitis A Antigen Isolated from Liver and Stool: Immunologic Comparison of Antisera Prepared in Guinea Pigs. Jules L. Dienstag, Alan N. Schulman, Robert J. Gerety, Jay H. Hoofnagle, Douglas E. Lorenz, Robert H. Purcell, and Lewellys F. Barker

950 Herpes Simplex-Raji (A44), A New Cell Line for Serologic Testing by Immunofluorescence. Jean-Marie Seigneurin, Claude Desgranges, Marie-France Lavoue, and Guy de The

953 Replication of Dengue-2 Virus in Cultured Human Lymphoblastoid Cells and Subpopulations of Human Peripheral Leukocytes. Argyrios N. Theofilopoulos, Walter E. Brandt, Philip K. Russell, and Frank J. Dixon

996 In Vitro Evaluation of Immunity to Coxiella burnetii. David J. Hinrichs and Thomas R. JerreUs 1004 Lymphocytic Choriomeningitis Virus-Induced Immunosuppression: A Virus-Induced Macrophage De-

fect. Robert P. Jacobs and Gerald A. Cole

COMMUNICATIONS

1054 Rapid Detection of Mycoplasma Antibody. Barbara L. Goldschmidt, Joseph P. Menonna, Peter C. Dowling, and Stuart D. Cook

1056 Announcements

1060 Errata

1061 Author Index

X CONTENTS

NUMBF.~R 4, OCTOBER 1976

[VOL. 117

CELLULAR IMMUNOLOGY

1063 The Suppressive Effect of Interferon on the Ability of Mouse Spleen Cells Synthesizing IgE to Sensitize Rat Skin for Heterologous Adoptive Cutaneous Anaphylaxis. Johnson Ngan, Spencer H. S. Lee, and Leon S. Kind

1067 Characterization of T and B Antigen-Binding Cells for ~-Galactosidase. III. Independence of Antigen- Binding Cells in Normal Animals from Antigenic Stimulation. Susan Swain and Albert H. Coons

1073 Effects of C-Reactive Protein on Platelet Function. II. Inhibition by CRP ofPlatelet Reactivities Stimu- lated by Poly-L-Lysine, ADP, Epinephrine, and Collagen. Barry A. Fiedel and Henry Gewurz

1093 Age-Associated Decline in the in Vitro Development of Cytotoxic Lymphocytes in NZB Mice. Toshio Hirano and Albert A. Nordin

1099 Appearance of Functional Lymphocytes in Fetal Liver. R.A. Phillips and F. Melchers 1111 Immunologic Cross-Reactions between the Specifically Localizing Cells (SLC) Generated in Response to

Sheep RBC and the Red Cells of Other Species. Donald R. Thursh and Keith S. Chan 1132 Coexistence of Helper and Suppressor Activities in Carrier-Primed Spleen Cells. Eva L. Chan and

Claudia Henry 1152 Specific, Transient Suppression of the Immune Response by HGG Tolerant Spleen Cells. II. Effector

Cells and Target Cells. Michael V. Doyle, D. Elliot Parks, and William O. Weigle 1159 Early and Late Contact Sensitivity Reactions in Guinea Pigs Sensitized to Oxazolone. Harold Baer,

Sanford H. Stone, and Frank Malik 1179 Differences in Ultrastructural Appearance and Modulation of Endogenous and Exogenous Surface

Immunoglobulin of Rabbit Lymphocytes. D. Scott Linthicum, Raja M. Bahu, and Stewart Sell 1211 Reaginic Antibody Formation in the Mouse. VIII. Depression of the Ongoing IgE Antibody Formation

by Suppressor T Cells. Kiyoshi Takatsu and Kimishige Ishizaka 1219 Differences in the Age-Dependent Release of a Low Molecular Weight Suppressor (LMWS) and

Stimulators by Normal and NZB/W Lymphoid Organs. David F. Ranney and Alfred D. Steinberg 1226 A Galactose-Inhibitable Mitogen for Human Lymphocytes from the Sponge Axinella pelypoides.

Stephanie Gordon Phillips, Hagen Bretting, and Elvin A. Kabat 1233 Functional Heterogeneity among the T-Derived Lymphocytes of the Mouse. V. Response Kinetics of

Peripheral T Cell Subpepulations. Barbara A. Araneo, Philippa C. Marrack, and John W. Kappler 1275 Mechanism of the Growth-Promoting Effect of Serum Albumin on Concanavalin A-Activated Lympho-

cytes: Protective Effect of the Plasma Proteins. Herman Polet and Helga Spieker-Polet 1288 Maturation of B Lymphocytes in Rats. III. Two Subpepulations of Memory B Cells in the Thoracic

Duct Lymph Differ by Size, Turnover Rate, and Surface Immunoglobulin. Samuel Strober 1308 Nucleoside Specificity in the Carrier IgG-Dependent Induction of Tolerance. B. David Stollar and Yves

Borel 1314 Quantitative Analysis of the Proliferative Activity Induced in Murine Thymocytes by Cencanavalin A.

Stanley Gerhart, Gordon Mills, Vicky Monticone, and Verner Paetkau 1320 Proliferation of Murine Thymic Lymphocytes in Vitro Is Mediated by the Concanavalin A-Induced Re-

lease of a Lymphokine (Costimulater). Verner Paetkau, Gordon Mills, Stanley Gerhart, and Vicky Monticone

1325 The Role of Macrophages in Thymocyte Mitogenesis. Gordon Mills, Vicky Monticone, and Verner Paetkou

1336 Mitogen-Stimulated Glutaraldehyde-Fixed Spleen Cells: Ability to Stimulate in the Mixed Lymphocyte Reaction and Generate Effector Cells in Ce, ll-Mediated Lympholysis. James L. Lightbody and Yi-chi M. Kong

1370 Regulatory Effect of Interferon on T Cells in Vitro. Iver Heron, Kurt Berg, and Karl CanteU

CLINICAL IMMUNOLOGY

1139 The Metabolism of IgE; Studies in Normal Individuals and in a Patient with IgE Myeloma. Thomas A. Waldmann, Atsushi Iio, Makio Ogawa, O. Ross Mclntyre, and Warren Strober

1303 Cbemotactic and Bactericidal Capacities of Human Newborn Monocytes. Roberto R. Kretschmer, Prudence B. Stewardson, Cynthia K. Papierniak, and Samuel P. Gotof[

IMMUNOCHEMISTRY

1117 Leukocyte-Derived Complement Inhibitor. IV. The Functional Properties of CI Bound to Erythrocytes Pretreated with Leukocyte Culture Supernatant. Alain Bernard, William Walter, Hideki Teshima, Laurence BoumseU, Robert A. Good, and Noorbibi K. Day

1189 Cross-Reactions between 2,4-Dinitrophenyl and Menadione (Vitamin K3) and the General Problem of Antibody Specificity. Marilyn F. M. Johnston and Herman IV. Eisen

1256 Activation of the Alternative Complement Pathway by Water-Insoluble Glucans of Streptococcus mutans: The Relation between Their Chemical Structures and Activating Potencies. Shinya lnai, Kazuyoshi Nagaki, Shigeyuki Ebisu, Keijiro Kato, Shozo Kotani, and Akira Misaki

1374 Solubilization of IgA Immune Precipitates by Complement. Gary W. Miller

1976]

1387

CONTENTS

Comparison of the Inactivation of IgM and IgG Complement Fixation Sites by Acid and Base. Stollar, Miguel J. Stadecker, and Shoshana Morecki

B. David

xi

IMMUNOGENICS AND TRANSPLANTATION

1079 Genetic Control of the Immune Response in Rats to the Known Sequential Polypeptide, (Tyr-Glu-Ala- Gly)n. I. Antibody Responses. Albert A. Luderer, Paul H. Maurer, and Robert T. Woodland

1197 Distinct Target Determinants on Two Lymphoblastoid Lines Derived from the Same Individual. Yukio Koide and Mitsuo Takasugi

1261 Macrophage-Lymphocyte Interaction. III. Site of Alloantiserum Inhibition of T Lymphocyte Prolifera- tion Induced by Allogeneic or Aldehyde-Rearing cells. Dirk K. Greineder, Ethan M. Shevach, and Alan S. Rosenthal

1295 Mixed Lymphocyte Reactions: Augmentation by Drugs That Inhibit Stimulator Cell Protein Syn- thesis. Charles M. Metzler, Thomas G. Kostyk, and Richard K. Gershon

1300 B Lymphocyte Alloantigen Specificities Present on Cultured Lymphoblastoid Cell Lines. R. Honig, R. Billing, M. Matsuyarna, and P. I. Terasaki

1351 Characterization of Ia Antigens in Mouse Serum. Gerald N. Callahan, Soldano Ferrone, M. David Poulik, Ralph A. Reisfeld, and Jan Klein

1363 In Vitro Responses of CBA/N Mice: Spleen Cells of Mice with an X-Linked Defect That Precludes Im- mune Responses to Several Thymus-Independent Antigens Can Respond to TNP-Lipepelysaccharide. D. E. Mosier, I. Scher, and W. E. Paul

IMMUNOPATHOLOGY

1085 Metabolic Studies of Guinea Pig Basophilic Leukocytes in Short-Term Tissue Culture. I. Measurement of Histamine-Synthesizing Capacity by Using an Isotopic-Thin Layer Chromatographic Assay. Stephen J. Galli, Anne S. Galli, Ann M. Dvorak, and Harold F. Dvorak

1164 Perturbation of Lymphocyte Circulation in Experimental Murine Leprosy. I. Description of the Defect. Ward E. Bullock, Jr.

1171 Perturbation of Lymphocyte Circulation in Experimental Murine Leprosy. II. Nature of the Defect. Ward E. Bullock, Jr.

1203 Demonstration of Human Nephritogenic Tubular Antigen in the Serum and Organs by Radioimmuno- assay. Yuzo Miyakawa, Kiyoshi Kitarnura, Seiichi Shibata, and Takuji Naruse

1238 Comparison of the Pyrogenicity, Limulus Activity, Mitogenicity, and Complement Reactivity of Several Bacterial Endotoxins and Related Compounds. Ronald J. Elin, Ann L. Sandberg, and David L. R ~sen- streich

1282 Independent Effects of IgG and Complement upon Human Polymorphonuclear Leukocyte Function. Ira M. Goldstein, Howard B. Kaplan, Allen Radin, and Matthew Frosch

1356 Release of Chemical Mediators from Partially Purified Human Lung Mast Cells. Nigel A. M. Pater- son, Stephen I. Wasserrnan, Jonathan W. Said, and K. Frank Austen

1378 Generation of a Neutrophil Chemotactic Agent by Spermatozoa: Role of Complement and Regulation by Seminal Plasma Factors. Robert A. Clark and Seyrnour J. Klebanoff

TUMOR IMMUNOLOGY

1145 Inhibition of Normal Allogenic Lymphocyte Mitogenesis by a Soluble Inhibitor Extracted from Human Colonic Carcinoma. Maryse M. Rernacle-Bonnet, Gilbert J. Pornrnier, Solange Kaplanski, Roselyne J. Rance, and Raymond C. Depieds

1243 Depression of Macrophage Function by a Factor Produced by Neoplasms: A Mechanism for Abrogation of Immune Surveillance. Marilyn C. Pike and Ralph Snyderrnan

1267 Characterization of Ia8 Antigen, Thy-l.2 Antigen, Complement Receptors, and Virus Production in a Group of Murine Virus-Induced Leukemia Cell Lines. Bruce Chesebro, Kathy Wehrly, Kevin Chesebro, and John Portis

1331 Reversal of Cell Surface Abnormalities of T Lymphecytes in Hodgkin's Disease after in Vitro Incuba- tion in Fetal Sera. Zvi Fuks, Samuel Strober, Donna P. King, and Henry S. Kaplan

1340 Nonspecific Inhibition of Tumor Growth in Vivo by Admixed Allogeneic Tumor- Sensitized Lymphoid Cells and Identical Inactivated Allogeneic Tumor Cells. F. Sendo, M. Nakayama, E. Gotohda, and H. Kobayashi

1346 Lysis of Tumor cells by Antibody and Complement. VII. Complement~Dependenta6Rb Release--a Nonlethal Event? Michael D. P. Boyle, Sarkis H. Ohanian, and Tibor Borsos


1104 Virus-Enhanced Modulation of Cell Surface Antigens: Effect on Immune Lytic Susceptibility. PhiUip J. Catanzara, Waiter E. Brandt, Wayne R. Hogrefe, Stanley M. Phillips, and Franklin H. Top, Jr.

xii CONTENTS [VOL. 117

1127 Hypergammaglobulinemia in Chickens Congenitally Infected with an Avian Leukosis Virus. Louis F. Qualtiere and Paul Meyers

1250 Xenogenization of Rat Erythroid Cells by Lymphatic Leukemia Virus: Its Role in Induction of Auto- immune Hemolytic Anemia. Noboru Kuzumaki, Tetsuya Moriuchi, Takao Kodama, and Hiroshi Kobayashi

1392 Large-Scale Isolation of Dane Particles from Plasma Containing Hepatitis B Antigen and Demonstra- tion of a Circular Double-Stranded DNA Molecule Extruding Directly from Their Cores. Tahashi Takahashi, Susumu Nakagawa, Takashi Hashimoto, Kazuaki Takahashi, Mitsunobu lmai, Yuzo Miyakawa, and Makoto Mayumi

1398 Primary in Vitro Sensitization of Murine Lymphocytes against Isogeneie and Allogeneic Cells Trans- formed by Simian Virus 40. Takashi Maki and Michael L. Howe

COMMUNICATIONS

1402 Autologous Human Cellular Cytotoxicity Induced by Mitogenic and Nonmitogenic Loctins. Richard P. MacDermott, Geoffrey S. Nash, John G. Saint, Eloise A. Clark, Alexander G. Zaras, and David Boldt

1404 Characterization of Antibodies to the Structural Polypeptides of HB~AG: Evidence for Subtype-Specific Determinants. Jon W. M. Gold, J. Wai-Kuo Shih, Robert H. Purcell, and John L. Gerin

1406 Partial Separation of Human Blood Leukocytes by Density Gradient Electroph0resis: Different Mobili- ties of Lymphocytos with IgG, Those with IgM and IgD, T L ymphoeytos, and Monocytos. Kenneth A. Ault, Ann L. Griffith, Christos D Platsoucas, and Nicholas Catsimpoolas

1409 Announcements 1410 Erratum 1411 Author Index

NUMBER 5, PART 1, NOVEMBER 1976

1413 Presidential Address

CELLULAR IMMUNOLOGY

1429 Characterization of Lymphocyte Inhibition by Supernatants of Crowded Lymphocytoblasts. Stanley S. Green and Richard Wistar, Jr.

1434 A Human T-Independent B Lymphocyte Mitogen Extracted from Nocardia opaca. Jean Brochier, Constantin Bona, Rita Ciorbaru, Jean-Pierre ReviUard, and Louis Chedid

1464 Interaction of K Lymphocytes with Myeloma Proteins of Different IgG Subclasses. Hans L. Spiegel- berg, Hedvig Perlrnann, and Peter Perlmann

1503 Identification of Multiple Cytolytic Components Associated with the ~-LT Class of Lymphotoxins Released by Mitogen-Activated Human Lymphocytes in Vitro. John C. Hiserodt, Daryl S. Fair, and G. A. Granger

1519 The Induction of Hapten-Specific T Cell Tolerance by Using Haptea-Modified Lymphoid Cells. I. Characteristics of Tolerance Induction. Stephen D. Miller and Henry N. Claman

1527 Anti-immunoglobulin Stimulation of Murine Lymphocytes. II. Identification of Cell Surface Target Molecules and Requirements for Cross-Linkage. Howard L. Weiner, John W. Moorhead, Karen Yamaga, and Ralph T. Kubo

1538 The T Cell Dependence of B Cell Differentiation Induced by Pokeweed Mitogen. Richard G. Keightley, Max D. Cooper, and Alexander R. Lawton

1594 The Induction and Regulation of Guinea Pig B Lymphocyte Proliferation in Vitro. Peter E. Lipsky and Alan S. Rosenthal

1603 Analysis of the Cross-reactive Immune Suppression Induced by the Random Copolymers L-Glutamic Acid 5°- L-Tyrosine" (GT), L-Glutamic Acid~°-L-Alanine '° (GA), and L-Glutamic Acid 6°- L-Alanine"- L-Tyrosine ~° (GAT). Carl Waltenbaugh, Patrice Debr~, and Baruj Benacerra[

1608 Regulation of the Primary Immune Response in Vivo by Parathyroid Hormone. S.H.H. Swierenga, J. P. MacManus, B. M. Braceland, and T. Youdale

1618 Ontogeny of the Antibody-Forming Cell Line in Mice. II. Maturation of B Cells during Fetal Develop- meat. Yvonne J. Rosenberg and Alastair J. Cunningham

1622 Hapten-Specific IgE Antibody Responses in Mice. V. Differential Resistance of IgE and IgG B Lympho- cytes to X-Irradiation. David A. Fox, Nicholas Chiorazzi, and David H. Katz

1629 Hapten-Specific IgE Antibody Responses in Mice. VI. Selective Enhancement of IgE Antibody Production by Low Doses of X-Irradiation and by Cyclophosphamide. Nicholas Chiorazzi, David A. Fox, and David H. Katz

1644 Supl, ression of in Vitro Lymphocyte Stimulation in Mice by Uterine and Placental Extracts. Nicola M. Kouttab, Arnold K. Fowler, James E. Strickland, and Alfred HeUman

1651 Guinea Pig Lymphocyte-Derived Macrophage Aggregation Factor: Its Separation from Macrophage Migration Inhibitory Factor. Arnold E. Postlethwaite and Andrew H. Kang

1656 The Involvement of Cyclic Nucleotide Metabolism in the Initiation of Lymphocyte Proliferation Induced by Mitogens. James Watson

1711 Carrier-Dependent Selection of Antibody Clones and Classes during the Immune Response. H. Kolb

1976]

1716

1721

CONTENTS

Characterization of Macrophage Migration Inhibitory Factor Activity Produced in Vivo by a Cell- Mediated Immune Reaction in the Guinea Pig. Arnold E. Postlethwaite, Alexander S. Townes, and Andrew H. Kang Antibedy-Mediated Basophil Accumulations in Cutaneous Hypersensitivity Reactions of Guinea Pigs. Philip W. Askenase, J. David Haynes, and Betty J. Hayden

CLINICAL IMMUNOLOGY

1472 The Effector Cells in Human Peripheral Blood Mediating Mitogen-Induced Cellular Cytotoxicity and Antibody-Dependent Cellular Cytotoxicity. David L. Nelson, Bonita M. Bundy, Howard E. Pitchon, R. Michael Blaese, and Warren Strober

1684 Abnormalities of Cellular Immune Response in Arthritis Induced by Rubella Vaccination. Yasuo Chiba, Esmaeel Sadeghi, and Pearay L. Ogra

1705 The Inhibition of Initial Steps of Lymphocyte Transformation by Cytochalasin B. Klaus Resch, Marlot Prester, Ernst Ferber, and Erwin W. Gelfand

1731 Identification of T and B CoIl Subpopulations in Human Peripheral Blood: Electrophoretic Mobility Distributions Associated with Surface Marker Characteristics. Joel H. Kaplan and E. E. Uzgiris

1440

1482

1491

1567

1573

1579

1638

1664

1688

1695

1456

1495

1507

1514

1532

:1584

1589

1668

1447

IMMUNOCHEMISTRY

Activation of the Complement Attack Mechanism in the Fluid Phase and Its Control by C5-~-INH: Lysis of Normal Erythrocytes Initiated by Zymosan, Endotoxin, and Immune Complexes. Thomas F. Lint, Carole L. Behrends, Patricia J. Baker, and Henry Gewurz Cell Membrane Antigen Isolation with the Staphylococcal Protein A-Antibody Adsorbent. Steven W. Kessler Investigations of the Complement-Fixing Sites of Immunoglobulins. Brian J. Johnson and Kenneth E. Thames B COIl Differentiation Induced by Lipopolysaccharide. IV. Development of Immunoglobulin Class Restriction in Precursors of IgG-Synthesizing Cells. John F. Kearney, Max D. Cooper, and Alexander R. Lawton Disparity of IgE Binding between Normal and Tumor Mouse Mast Cells. GuiUermo R. Mendoza and Henry Metzger Cell Surface Immunoglobulin. XIX. Susceptibility of IgD and IgM on Murine Splenocytes to Cleavage by Papain. Ellen S. Vitetta and Jonathan W. Uhr Contrasting Effects of BCG on Spleen and Lymph Node Antibody Responses in Nude and Normal Mice. Bernard H. Dolt, Bruce Merchant, Lynne Johannessen, Sotiros D. Chaparas, and Neal A. Sher Immunogenicity of Fab Fragment of Protein-315 for BALB/c Mice. Rachaneepas Tungkanak and Stitaya Sirisinha Partial Characterization of Human C5a Anaphylatoxin. I. Chemical Description of the Carbohydrate and Polypeptide Portions of Human C5a. Horacio N. Fernandez and Tony E. Hugli Hapten-Sandwich Labeling. III. Bifunctional Reagents for Immunospecific Labeling of Cell Surface Antigens. Salvatore Cammisuli and Leon Wo[sy


Genetic Control of Ligand-Induced Events in B Lymphocytes. Robert Jo Fram, Charles L. Sidman, and Emil R. Unanue H-2 Compatibility Requirement for Virus-Specific T Cell-Mediated Effector Functions in Vivo. 1. Speci- ficity of T Cells Conferring Antiviral Protection against Lymphocytic Choriomeningitis Virus Is Asso- ciated with H-2K and H-2D. Roll M. Zinkernagel and Raymond M. Welsh Characterization of a Constitutive Variant of the Murine Serum Protein Allotype, Sip. Ted H. Hansen and Donald C. Shreffler Mapping of the Genetic Control of Murine Response to Low Doses of the Dinitrophenyl Conjugates of Ovomucoid and Bovine T-Globulin. John H. Freed, Beverly D. Deak, and Hugh O. McDevitt Relationship between B2-Microglobulin and Cell-Surface Alloantigens of the Mouse. RoyGeib, Miroslav D. Poulik, Ellen S. Vitetta, J. F. Kearney, and Jan Klein Relationship of Functional Levels of Early Components of Complement to the H-2 Complex of Mice. Margaret B. Goldman and John N. Goldman Isolation and Characterization of Human B Cell Alloantigens. Ronald J. Billing, Madrid Safani, and Patricia Peterson IgA Half Molecules. II. Genetic Variants of IgA Detected in Normal Mouse Intestinal Contents. J. Frederic Mushinski and Miranda M. Dungan

IMMUNOPATHOLOGY

Thyroid Antibodies in Spontaneous Autoimmune Thyroiditis in the Buffalo Rat. Yoshida, Noel R. Rose, and Pierluigi E. Bigazzi

Bernice Noble, Takeshi

o ° .

Xlll

xiv CONTENTS [VOL. 117

1545 Serotonin-lnduced Production of a Monocyte Chemotactic Factor by Human Peripheral Blood Leukocytes. Kenneth A. Foon, Sharon M. Wahl, Joost J. Oppenheim, and David L. Rosenstreich

1553 Schistosoma mansoni Soluble Egg Antigens: I. Identification and Purification of Three Major Antigens, and the Employment of Radioimmunoassay for Their Further Characterization. Ronald P. PeUey, Robert J. Pelley, Joseph Hamburger, Pierre A. Peters, and Kenneth S. Warren

1561 Sehistosoma mansoni Soluble Egg Antigens. II. Determination of the Stage and Species Specificity of Their Serologic Reactivity by Radioimmunoassay. Joseph Hamburger, Ronald P. Pelley, and Kenneth S. Warren

1676 Chemotaxis of Human Polymorphonuclear Neutrophils under Agarose: Morphologic Changes Associ- ated with the Chemotactic Response. Robert D. Nelson, Vance D. Fiegel, and Richard L. Simmons

1612

TUMOR IMMUNOLOGY

Synergism between Immune Complexes and Serum from Tumor-Bearing Mice in the Suppression of Mitogen Responses. Douglas G. Kilburn, Maureen Fairhurst, Julia G. Levy, and Richard B. Whitney

ERRATA

1721 Antibody-Mediated Basophil Accumulations in Cutaneous Hypersensitivity Reactions of Guinea Pigs. Philip W. Askenase, J. David Haynes, and Betty J. Hayden

1731 Identification of T and B Cell Subpopulations in Human Peripheral Blood: Electrophoretic Mobility Distributions Associated with Surface Marker Characteristics. Joel H. Kaplan and E. E. Uzgiris

NUMBER 5, PART 2, NOVEMBER 1976

CELLULAR IMMUNOLOGY

1749 Evidence for Separate Subpopulations of B Cells Responding to T-Independent and T-Dependent Im- munogens. John J. Jennings and Marvin B. Rittenberg

1774 Membrane Expression of Thy-l.2 and G M, Ganglioside on Differentiating T Lymphocytes. Chris- tine Milewicz, Harold C. Miller, and Walter J. Esselman

1817 Induction or Suppression of B Cell Proliferation and Differentiation by Phytohemagglutinin or Con- canavalin A in Mouse Spleen Cell Cultures. Pierre-F. Piguet, Henry K. Dewey, and Pierre VassaUi

1824 Antibodies to Guinea Pig Lymphokines. IV. Suppression of the Mixed Leukocyte Culture Reaction by Anti-lymphokine Globulin. Carolyn L. Geczy, Andrew F. Geczy, and Alain L. de Weck

1853 Cell Interactions between Histoineompatible T and B Lymphocytes. IX. The Failure of Histoincom- patible Cells is Not Due to Suppression and Cannot be Circumvented by Carrier-Priming T Cells with Allogeneic Macrophages. David H. Katz, Nicholas Chiorazzi, Jeanne McDonald, and Lee R. Katz

1927 Response of Human Lymphocytes to PHA and Con A, Dependent on and Regulated by THF, A Thymic Hormone. Varda Rotter, Menachem Schlesinger, Rina Kalderon, and Nathan Trainin

1938 Two Types of Antibody-Dependent Cell-Mediated Cytotoxicity, Arming and Sensitization. T. Imir, Eero Saksela, and O. MakeM

1949 Electrophoretic Separation of Radioisotopically Labeled Mouse Lymphocytes. A.L. Griffith, N. Cat- simDoolas, M. K. Dewanjee, and H. H. Wortis

1953 Antigen-Specific and Nonspecific Mediators of T CelI/B Cell Cooperation: IV. Development of a

Model System Demonstrating Responsiveness of Two T Cell Functions to HGG in Vitro. J. Terrell Hoffeld, Philippa Marrack, and John W. Kappler

1960 Antigen-Specific and Nonspecific Mediators of T CelI/B Cell Cooperation: V. Unresponsiveness to HGG in Vitro of these Two T Cell Subpopulations. J. TerreU Hoffeld, Philippa Marrack, and John W. Kappler

1976 Isolation of a Human Lymphocyte Mitogen from Wheat Germ with N-Acetyl-D-Glucosamine Specific- ity. John M. Brown, Myron A. Leon, and James J. Lightbody

1985 Anti-lymphokine Antibody. II. Specificity of Biological Activity. Tadatoshi Kuratsuji, Takeshi Yoshida, and Stanley Cohen

2014 T and B Lymphocytes in the Regulation of Delayed Hypersensitivity. Ruth Neta and S. B. Salvin 2021 Triggering of Human Peripheral Blood B Cells: Polyelonal Induction and Modulation of an in Vitro

PFC Response. Steven M. Friedman, Jacqueline M. Breard, and Leonard Chess

CLINICAL IMMUNOLOGY

1781 Studies of Surface Immunoglobulins on Human B Lymphocytes. II. Characterization of a Population of Lymphocytes Lacking Surface Immunoglobulins but Carrying Fc Receptor (SIg-Fc + Cell). Toru Abo, Tai Yamaguchi, Fumio Shimizu, and Katsuo Kumagai

1866 Activation of the Alternative Pathway of the Complement System by Radiographic Contrast Media. Carlos M. Arroyave, K. Naras Bhat, and Ronald Crown

1890 Prearmed Effector Cells and the Target Cell Specificity of Lectin-Induced Cellular Cytotoxicity. Richard P. MacDermott, Geoffrey S. Nash, and David Boldt

1906 Association of Histocompatibility Haplotype HI~-A3-B7 with Multiple Sclerosis. Hans J. Bertrams and Ernst K. Kuwert

2006 Cytomegalovirus Specific IgM and IgG Response in Humans Studied by Radioimmunoassay. Violeta Knez, John A. Stewart, and Donald W. Ziegler

1976] CONTENTS xv

1799

1805

1812

1838

1883

1898

1933

1971

IMMUNOCHEMISTRY

Mouse Factor B of the Alternative Pathway of Complement Activation. I. Purification, Characteriza- tion, and Functional Behavior. Dieter Bitter-Suermann, Reinhard Burger, Volker Brade, and Ulrich Hadding Complement Receptor Lymphocytes in the Rabbit. I. An SIg-Negative Subpopulation in the Appendix. William R. Green and Michael W. Fanger Complement Receptor Lymphocytes in the Rabbit. II. The Effect of Immunization on the Complement Receptor Lymphocyte Population in the Draining Lymph Node. William R. Green and Michael W. Fanger Binding of IgE Myeloma Proteins to Human Cultured Lymphoblastoid Cells. Andres Gonzalez- Molina and Hans L. Spiegelberg Complement-Induced Ultrastructural Membrane Lesions: Requirement for Terminal Components. Charles H. Packman, Stephen I. Rosenfeld, Robert I. Weed, and John P. Leddy Comparative Immunogenic Properties of N-Substituted Phosphatidylethanolamine Derivatives and Liposomal Model Membranes. Robert A. Nicolotti, Naohisa Kochibe, and Stephen C. Kinsky Preparation and Subfractionation of Isologous and Heterologous Anti-idiotypes with FV Fragments. Miriam Helman, Irit Shreier, and David Givol Specific Extraction of Antigen in Vivo by Extracorporeal Circulation over Antibody Immobilized in Collodion-Charcoal. David S. Terman, Donald Petty, Daryl Ogden, Carol Pefley, and George Buffaloe


1913 Reduction of the in Vitro Cytotoxic Lymphocyte Response Produced by in Vivo Exposure to Semi- allogeneic Cells: Recruitment or Active Suppression? Richard G. Miller and Robert A. Phillips

1922 Studies on Non-H-2-Liuked Lymphocyte-Activating Determinants. II. Nonexpression of Mls Deter- minants in a Mouse Strain with an X-linked B Lymphocyte Immune Defect. Aftab Ahrned and Irwin Scher

1966 Mutant Lines of Guinea Pig L2C Leukemia. III. The Reaction of an Alloantiserum Detecting Idiotypic Determinants on a Clonally Derived Guinea Pig B Cell Leukemia with IgM and Ia Molecules. T.G. Konen, I. Green, E. M. Shevach, A. M. Kask, and B. D. Schwartz

IMMUNOPATHOLOGY

1762 Relationship between the C5 Peptides Chemotactic for Leukocytes and Tumor Cells. Alberto G. Romualdez, Jr., Peter A. Ward, and Takaatsu Torikata

1878 Role of T Lymphocytes in Adjuvant Arthritis. I. Evidence for the Regulatory Function of Thymus- Derived Cells in the Induction of the Disease. Kohji Kayashima, Toshitaka Koga, and Kaoru Onoue

1894 Immunopathogenesis of Autoimmune Tubulointerstitial Nephritis. II. Role of an Immune Response Gene Linked to the Major Histocompatibility Complex. Lawrence R. Hyrnan, Alfred D. Steinberg, Robert B. Colvin, and Eugene F. Bernard

TUMOR IMMUNOLOGY

1743 Antibody. Response to Lymphoma Antigens in Syngeneic and H-2 Compatible Mice. James G. Dalton, Shigeyoshi Fujimoto, and Alec H. Sehon

1767 Stimulation of Immune Mechanisms against Mammary Tumors by Incomplete T Cell Depletion. Jirayr R. Roubinian, Mary-Ann Lane, Maxwell Slomich, and Phyllis B. Blair

1832 Effect of a-Fetoprotein and Other Serum Factors Derived from Hepatoma-Bearing Rats on the Mixed Lymphocyte Response. Michael J. Parmely and John S. Thompson

1846 Purification and Partial Characterization of a Tumor-Specific Blocking Factor from Sera of Mice with Growing Chemically Induced Sarcomas. Jerry T. Neporn, Ingegerd HeUstr(~m, and Karl Erik Hell- strum

1860 Resistance to Tumor Growth Mediated by Listeria Monocytogenes. Collaborative and Suppressive Maerophage-Lymphocyte Interactions in Vitro. Said Youdim and Marti Sharman

1870 Analysis of Murine Oncofetal Antigens as Tumor-Associated Transplantation Antigens. Stanley E. Chism, Sally WaUis, Robert C. Burton, and Noel L. Warner

1903 The L1210 Leukemia Cell Bears a B Lymphocyte Specific, non-H-2 Linked Alloantigen. John G. Freund, Aftab Ahmed, Richard E. Budd, Martin E. Dor[, Kenneth W. Sell, Wilton E. Vannier, and Robert E. Humphreys

1943 Specific Lysis of Human Colon Tumor Cells by Antibodies to CEA and Isoantigen A: Dependence on Rabbit Serum or Neuraminidase. Wayne A. F. Tompkins, Pradeep B. Seth, Dick-Man Yip, Jerry L. Palmer, Sydney R. Gee, and William E. Rawls

1992 Possible Evidence for Antibody-Dependent Macrophage-Mediated Cytotoxicity Directed against Mur- ine Adenocarcinoma Coils in Vivo. J. Stephen HaskiU and James W. Fett


1753 Replication of Herpes Simplex Virus in Mouse Spleen Cell Cultures Stimulated by Lipopolysaccharide. Holger Kirchner, Hans-Martin Hirt, Christa Kleinicke, and Klaus Munk

1757 Inhibition of Yaccinia Virus Replication in Skin of Tuberculin-Sensitized Animals Challenged with PPD. Donald L. LodmeU, Larry C. Ewalt, and Abner Louis Notkins

xvi CONTENTS [VOL. 117

1788 Analysis of Parameters Affecting the Solid Phase Radioimmunoassay Quantitat ion of Antibody to Meningococcal Antigens. Wendell D. ZoUinger, Joel M. Dalrymple, and Malcolm S. Artenstein

1981 In Vitro Synthesis of Immunoglobulin and Anti-viral Antibody in Aleutian Disease Viral Infection of Mink. Lance E. Perryman, Travis C. McGuire, and Keith L. Banks

1999 Immunity to Phasmodium Berghei Yoelii in Mice. I. The Course of Infection in T Cell and B Cell De- ficient Mice. Fredric I. Weinbaum, Charles B. Evans, and Robert E. Tigelaar

2029 Natura l Immunity in Cats to Feline Leukemia Viral Antigens. Sandra K. Ruscetti, and Wade P. Parks

2036

2038

2040

2042

2045 2O46

COMMUNICATIONS

Acquired Resistance to Infection in an Animal Model of Giardiasis. Ian C. Roberts-Thomson, David P. Stevens, Adel A. F. Mahmoud, and Kenneth S. Warren Induction of Antibody to the "y" Determinant of HB~ Ag in a Chimpanzee Carrier of HB, Ag Subtype " a d w " . E. Tabor, R. J. Gerety, L. A. SmaUwood, and L. F. Barker Blocking of HLA and B Lymphocyte Alloantigens with F(ab' )2 Fragments of Rabbi t Antibodies. Ronald J. Billing, Ronald Honig, Patricia Peterson, Madrid Safani, and Paul L Terasaki Lack of Antibody Activity Directed Against the Most Common Human Bacteria in Human Myeloma Protein. Jacques PiUot, Nicole Creau-Goldberg, and Yolanda Gonzales Announcements Author Index

NUMBER 6, DECEMBER 1976

CELLULAR IMMUNOLOGY

2119 Analysis of Mononuclear Cell Surfaces with Fluoresceinated Staphylococcal Protein A Complexed with IgG Antibody or Heat-Aggregated 7-Globulin. Edwin W. Ades, Don J. Phillips, &even L. Shore, David S. Gordon, Mar&no F. LaVia, Charlotte M. Black, and Charles B. Reimer

2131 Functional Heterogeneity among the T-Derived Lymphocytes of the Mouse. VI. Memory T Cells Stored in the T2 Subpopulation. Barbara A. Araneo, Philippa C. Marrak, and John W. Kappler

2137 Mouse B and T Lymphocyte Responses to Purified Timothy Pollen Antigens in Vitro. SaUy S. Fairchild and Arthur Malley

2150 Effect of Vibrio Cholerae Neuraminidase on the Generation of Cell-Mediated Cytotoxicity in Vitro. Ronald Ferguson, Susan M. Anderson, Jon R. Schmidtke, and Richard L. Simmons

2158 The Regulation of the Immune Response to T-Independent Antigens by Prostaglandins and B Cells. Michal Zimecki and David R. Webb

2165 Tuberculin-Sensitized Lymphocytes Detected by Altered Electrophoretic Mobility Distributions after Incubation with the Antigen PPD. E. E. Uzgiris and Joel H. Kaplan

2175 Activation of Cytotexic Function in T Lymphocytes. Diane Heininger, Mary Touton, Ashim K. Chakra- varty, and William R. Clark

2204 Autoimmunity and Aging: The Age-Related Response of Mice of a Long-Lived Strain to Trinitrophenyl- ated Syngeneic Mouse Red Blood Cells. David Naor, Ben Bonavida, and Roy L. Walford

2209 Maintenance of Hyporesponsiveness to Antigen by a Distinct Subclass of T Lymphocytes. S. Weitzman, F. W. Shen, and H. Cantor

2213 Kinetic Analysis of Target Cell Destruction by Effector T Cells. I. Delineation of Parameters Related to the Frequency and Lytie Efficiency of Killer Cells. Richard M. Thorn and Christopher S. Henney

2220 Subpopulations of Chicken B Lymphocytes. John Lifter, Paul W. Kincade, and Yong Sung Choi 2226 Cell-Mediated Immune Response in Vitro. II. The Mechanism(s) Involved in the Suppression of the

Development of Cytotoxic Lymphocytes. Toshio Hirano and Albert A. Nordin

CLINICAL IMMUNOLOGY

2053 Relationship between Homogeneous IgG Fractions and Measles Virus Antibody Activities in Subacute Sclerosing Panencephalitis Brain. P. D. Mehta, A. Kane, and H. Thormar

2100 Activation of Human B Lymphocytes. II. Cellular Interactions in the PFC Response of Human Ton- sillar and Peripheral Blood B Lymphocytes to Pol~clonal Activation by Pokeweed Mitogen. Anthony S. Fauci, Karen R. K. Pratt, and Gall Whalen

2171 Subpopulations of Human T Cells Identified by Receptors for Immunoglobulins andMitogenRespon- siveness. Lorenzo Moretta, Mantio Ferrarini, Maria C. Mingari, Alessandro Moretta, and Susan R. Webb

IMMUNOCHEMISTRY

2181 The Role of Hydroxylation of Proline in the Antigenicity of Basement Membrane Collagen. Bradley W. Arbogast, Diane E. Gunson, and Nicholas A. Kefalides

2185 Structural and Genetic Studies on Chicken 7S Immunoglobulin Allotypes. I. Detection of Four Heavy Chain Specificities with a New Radioimmunoassay. Edward K. Wakeland and Albert A. Benedict

2249 Hemolytic Plaque Inhibition: The Physical-Chemical Limits on Its Use as an Affinity Assay. Charles DeLisi

1976 ] CONTENTS xvii


2047 Genetic Control of Immune Responsiveness to Poly(LPro)--Poly(LLys)-Derived Polypeptides by His- tocompatibility-Linked Immune Response Genes in the Rat. Eberhard G~inther, Edna Mozes, Erwin RCide, and Michael Sela

2061 Genetic Control of B Cell Activation by Bacter ial Lipopolysaceharide Is Mediated by Multiple Distinct Genes or Alleles. L. Michael Glode and David L. Rosenstreich

2092 The Genetics of Cell-Mediated Lympholysis. Mary Ann Long, Barry S. Handwerger, D. Bernard Amos, and EdmondJ. Yunis

2105 Idiotypes of Inulin-Binding Antibodies and Myeloma Proteins Controlled by Genes Linked to the Allo- type Locus of the Mouse. R. Lieberman, M. Potter, W. Humphrey, Jr., and C. C. Chien

2233 Minor H Antigens Introduced on H-2 Different Stimulating Cells Cross-React at the Cytotoxic T Cell Level during in Vivo Priming. Michael J. Bevan

2143

2191

IMMUNOPATHOLOGY

Trypsin-Induced Increase in Intracel lular Cyclic AMP of Lymphocytes. Amir Shneyour, Yehuda Patt, and Nathan Trainin Functional Characterizat ion of Rat Mast Cell Arylsulfatase Activity. Robert P. Orange and Elizabeth G. Moore

TUMOR IMMUNOLOGY

2073 Mechanism of Mastocytoma-Mediated Suppression of Lymphocyte Reactivity. Frank DeLustro and Bertie F. Argyris

2197 Cell-Mediated Destruction of Human Leukemic Cells by MHC Identical Lymphocytes: Requirement for a Proliferative Trigger in Vitro. Paul M. Sondel, Carmeline O'Brien, Linda Porter, Stuart F. Schloss- man, and Leonard Chess

2239 Immuni ty to Virus-Free Syngeneic Tumor Cell Transplanta t ion in the BALB/e Mouse after Im- munization with Homologous Tumor Cells Infected with Type C Virus. Ismail K. Al-Ghazzouli, Robert M. Donahoe, Kun-yen Huang, Bernard Sass, Robert L. Peters, and Gary J. Kelloff


2067 Cytotoxic Macrophages: A Rapid Nonspecific Response to Viral Infection. Stuart J. Rodda and David O. VT~ite

2081 Antibody-Induced Modulation of Leishmania donovani Surface Membrane Antigens. Dennis M. Dwyer

2112 Modification of H-2 Antigenic Sites by Enzymatic Trea tment Influences Virus-Specific Target Cell Lysis. Hildegund C. J. Ertl and Ulrich H. Koszinowski

2124 Use of Silica to Identify Host Mechanisms Involved in Suppression of Established Friend Virus Leukemia. Julia J. Wirth, Michael H. Levy, and E. Frederick Wheelock

COMMUNICATIONS

2258 Co-occurrence of Hepati t is B Surface Antigen of a Par t icular Subtype and Antibody to a Heterolo- gous Subtypic Specificity in the Same Serum. Takemichi Sasaki, Yasuhito Ohkubo, Yutaka Yamashita, Mitsunobu Imai, Yuzo Miyakawa, and Makoto Mayumi

2260 The Development of HB~Ag-Positive Hepati t is Despite the Previous Existence of Antibody to HBsAg. Deloris E. Koziol, Harvey J. Alter, John P. Kirchner, and Paul V. Holland

2262 Concurrently Circulating Hepati t is B Surface Antigen and Heterotypic Anti-HBs Antibody. George L. Le Bouvier, Roy A. Capper, Alan E. Williams, Marie Pelletier, and Alfred J. Katz

2264 Specificity of the in Vitro Immune Response to Synthetic Polypeptide Antigens in Inbred Rats. Jon K. Minford, Donald V. Cramer, and ThomasJ. Gill, lIl

2267 Experimental Autoimmune Thyroiditis in the Guinea Pig: Characterizat ion of Infi l t rat ing Lym- phocyte Populations. Stephen A. Paget, Philip R. B. McMaster, and John A. van Boxel

2270 Letter to the Editor 2274 Announcements 2273 Author Index 2275 Author Index Volume 117 2280 Subject Index Volume 117

THE JOURNAL OF IMMUNOLOGY

1 . N e w . " " IMMUNOHISTOPATHOLOGY By YOSHIHIRO HAMASHIMA, M.D. This is a definitive study of the pathological aspects of the body's immune processes. Explicit microscopic studies of immune reactions, obtained by introducing fluorescent- and enzyme-labelled antibodies into tissues, clearly show morphological changes resulting from immunopathological processes in over 100 full-color photomicrographs. Content ranges from processes and substances basic to all immunological functions to detailed study of the immunopathology of various body systems and disorders. 308 pages / 145 illustrations, 127 in color / 1976 / $62.00

2 . N e w . " " IMMUNOLOGY OF PARASITIC INFECTIONS

Edited by SIDNEY COHEN, M.D., Ph.D., F.R.C.Path.; and the late ELVIO H. SADUN, Ph.D. This book promotes greater interchange between para- sitologists and immunologists in the hope that serious problems of parasitic infections in many tropical and sub- tropical countries may eventually be overcome. 498 pages / illustrated / 1976 / $46.00

6. ESSENTIAL IMMUNOLOGY 2nd Edi t ion

By IVAN M. ROITT, M.A., D.Sc., M.R.C.Path. This updated edition of a concise book offers a modern view on a rapidly changing subject. It establishes the language and essential phenomena of immunology and their implications in disease. Emphasis is on clear exposi- tion of the essential principles and a broad summary of their importance in medicine. 260 pages / 136 illustrations / revised 2nd printing of 2nd edition, 1975 / soft cover, $10.65

HANDBOOK OF EXPERIMENTAL IMMUNOLOGY. • • 2nd Edi t ion Edited by D. M. WEIR, M.D. Available in three soft-cover volumes, this book provides background information and descriptions of procedures of value to those who use immunological methods.

1070 pages / 473 illustrations / 2nd edition, 1973

7. Vol . 1, IMMUNOCHEMISTRY, $32.00

3. N e w . • • PRACTICAL IMMUNOLOGY By LESLIE HUDSON; and FRANK C. HAY This is a step-by-step laboratory/classroom guide to ex- periments demonstrating modern immunological principles and processes. Coverage is from an in-depth ex- amination of basic lymphocyte units through the complete immune system. Includes precise instructions on current experimental techniques including direct and indirect immunofluorescence and radioimmunoassay. 266 pages / 30 illustrations / 1976 / soft cover, $20.00

4. N e w . • • INFECTION AND IMMUNOLOGY IN THE RHEUMATIC DISEASES

Edited by D. C. DUMONDE, M.A., Ph.D., M.B., B.Chir., M.R.C.Path., F.R.hC.; with 178 contributors. The knowledge of specialists from around the world is integrated in this book into an exhaustive study of the immunopathological basis of rheumatoid arthritis and allied diseases. It describes the mechanisms of pathological responses to microbial infection and genetic susceptibility to infectious disease. 692 pages / 235 illustrations / 184 tables / 1976 / $95.00

5. N e w . • • THE IMMUNE SYSTEM A C o u r s e on the M o l e c u l a r and Ce l lu la r Basis of I m m u n i t y Edited by M. a. HOBART, M.A., Ph.D., Vet.M.B.; and IAN McCONNELL, M.A., Ph.D., B.V.M.S.; with 14 contributors This is a detailed account of current immunology based on an integrated course of lectures presented at the Royal Postgraduate Medical School, University of London, by a number of leading immunologists. The text includes: Immunochemistry, Immunobiology, Immunogenetics, and Immunopathology. 357 pages / 116 illustrations, 56 tables / 1976 (U.S.) soft cover, $16.50

8. VoI. 2, CELLULAR IMMUNOLOGY, $26.50

9. Vol. 3, APPLICATION OF IMMUNOLOGICAL METHODS,

$26.50

10. ULTRASTRUCTURE OF LYMPHOID ORGANS

An Electron-Microscopic Atlas By I. OLAH, M.D., C.Sc.; P. ROHLICH, M.D., C.Sc.; and h TORO, M.D. This timely atlas describes and illustrates the microen- vironment in which immunocytes are produced, stored and activated, and in which cooperation between the various cell types occurs.

317 pages / 267 illustrations / 1975 / $30.00

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The purpose of this book is to bring recent progress in experimental immunology to the bedside in a manner that is both useful and comprehensible to practicing physicians, investigators and trainees in the various branches of internal medicine, pediatrics, laboratory medicine and surgery. It is also designed to be of assistance to physicians preparing for Board Examinations in Allergy and Immunology and to undergraduate students wishing to acquire specialized knowledge of applied immunology. The intention is to provide the reader with an appreciation of the etiology and pathogenesis of common immunologic disorders as well as with information concerning their clinical manifestations and treatment. New chapters have been added on immunotherapy and immunosuppression, mechanisms of protective immunity, and immunoassays in clinical medicine.

2. Immunolog lmmunopathology, and Immunity By Stewart Sell, M.D., Professor, Department of Pathology, School of Medicine, University of California at San Diego. 384 pages. 45 illustrations. Paperbound. $14.95 SECOND EDITION

The role of immune reactions in protection against infectious diseases and cancer are presented in a concise, highly organized manner. Intended as an introduction to immunology for undergraduates, graduate students, medical students, interns and residents. Includes classical immunologic subjects, such as characteristics of antigens, antibody structure and antibody-antigen reaction, and new information on cellular interactions in induction and expression of immune responses, the consequences of allergic reactions that cause immune mediated diseases.

3. immunology By Herman N. Eisen, M.D., Professor of Immunology, Massachusetts Institute of Technology, Cambridge. 624 pages. 260 illustrations. Paperbound. $14.50

Reprinted from the second edition of Davis et al.'s MICROBIOLOGY. This is a comprehensive introductory text which emphasizes the broad range of biochemical, genetic and clinical observations that provide the foundations for current concepts in immunology. Immune responses against foreign cells, microbes, and organic molecules are described in terms of specifically reactive antibodies and lymphoid cells. The molecular properties of antigens and antibodies are also described in detail.

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