The impact of fingerprint The impact of fingerprint reagent – cyanoacrylate on reagent – cyanoacrylate on

DNADNA

88thth International Symposium on Forensic Sciences International Symposium on Forensic SciencesŠamorín – ČilistovŠamorín – Čilistovseptember september 2727thth 2007 2007

Zuzana DeščíkováZuzana Deščíková

Aims of the presentationAims of the presentation

To describe an influence of fingerprint To describe an influence of fingerprint enhancement reagent – cyanoacrylate enhancement reagent – cyanoacrylate on DNA and to show, that it has no on DNA and to show, that it has no deleterious effect on DNAdeleterious effect on DNA

Structure of presentationStructure of presentation

1. History1. History2. Reality2. Reality3. Problem3. Problem4. Question4. Question5. Cyanoacrylate5. Cyanoacrylate6. DNA analysis6. DNA analysis7. Fingerprints or DNA?7. Fingerprints or DNA?8. Conclusion8. Conclusion

1. History1. History Ancient fingerprintsAncient fingerprints Fingerprint pioneersFingerprint pioneers- M.MalpighyM.Malpighy- J.E.PurkinjeJ.E.Purkinje- Sir W. HerschelSir W. Herschel- H. FauldsH. Faulds- A. BertillonA. Bertillon- Sir F. GaltonSir F. Galton- Sir HenrySir Henry Enhancement of Enhancement of

latent fingerprintslatent fingerprints

2. Reality2. Reality

Evidence from the Evidence from the crime scenecrime scene

Selection of the Selection of the right methodright method

Order of expertiseOrder of expertise

3. Problem3. Problem

Persistance of fingerprint / DNAPersistance of fingerprint / DNA Factors influencing the fingerprintsFactors influencing the fingerprints Time Time

Facts Facts - Year Year 20052005 3227 cases3227 cases

33%% together with DNA analysis together with DNA analysis

- Year Year 20062006 2931 cases2931 cases2,52,5%% together with DNA analysis together with DNA analysis

- Year Year 20072007 2178 cases 2178 cases 55%% together with DNA analysis together with DNA analysis

9090%% of these expertises was delayed app. for of these expertises was delayed app. for about 3 monthsabout 3 months

4. Question4. Question

Do chemicals and methods used in Do chemicals and methods used in dactyloscopy have negative influence dactyloscopy have negative influence on DNA?on DNA?

Is it possible to make fingerprint Is it possible to make fingerprint analysis BEFORE the DNA analysis?analysis BEFORE the DNA analysis?

How does cyanoacrylate influence the How does cyanoacrylate influence the DNA?DNA?

5. Cyanoacrylate5. Cyanoacrylate

„„Superglue“ or Superglue“ or „Krazy glue“„Krazy glue“

Ethyl-2-Ethyl-2-cyanoacrylatecyanoacrylate

CA fumes react CA fumes react with amino acidswith amino acids

Use for smooth, Use for smooth, nonporous surfacesnonporous surfaces

6. DNA analysis6. DNA analysis Same task as dactyloscopy- Same task as dactyloscopy-

individual identification individual identification - identification of suspectsidentification of suspects- exoneration of persons wrongly accused of exoneration of persons wrongly accused of

crimecrime- DVIDVI- establishing of the paternity etc.establishing of the paternity etc.

Serological analysis - RFLP – Serological analysis - RFLP – PCR using STR PCR using STR

Smaller samples, more Smaller samples, more sensitive analysis sensitive analysis

7. Fingerprints or DNA?7. Fingerprints or DNA?

Destructive influence of fingerprint Destructive influence of fingerprint enhancement chemicals on serological enhancement chemicals on serological teststests

No deleterious effect of CA and other No deleterious effect of CA and other fingerprint enhancement chemicals on fingerprint enhancement chemicals on subsequent DNA analysis of STRssubsequent DNA analysis of STRs

((Hochmeister et al., Stein et al., Zamir et al.Hochmeister et al., Stein et al., Zamir et al., Frégeau et al.), Frégeau et al.)

! Exceptions !! Exceptions !

Use with careUse with care - - physical developerphysical developer- shortwave UV light- shortwave UV light- destaining- destaining

DeleteriousDeleterious effect on DNA: effect on DNA:- Stickyside powder- Stickyside powder- Stickyside powder + Un-do- Stickyside powder + Un-do- prolonged exposure to blood reagents- prolonged exposure to blood reagents

8. Conclusion8. Conclusion

DNA profiles can still be obtained after DNA profiles can still be obtained after the use of the majority of fingerprint the use of the majority of fingerprint enhancement techniquesenhancement techniques

Fingerprint analysis of latent prints can Fingerprint analysis of latent prints can be done BEFORE the DNA analysis of be done BEFORE the DNA analysis of the same subjectthe same subject

Contact informationsContact informations

Zuzana DeščíkováZuzana Deščíková

Finger, palmprint and trasology departmentFinger, palmprint and trasology departmentInstitute of Forensic sciencesInstitute of Forensic sciences(421) 9610-57270(421) 9610-57270descikova@keupz.minv.skdescikova@keupz.minv.sk