The evaluation of Pre-Cut corneas prepared in The International Eye

Bank of Prague

Magdaléna Netuková, Yveta Urbanová, Josef Šach, Pavel Kuchynka

Ocular Tissue Bank OTB01/The International Eye Bank of Prague, Czech RepublicDpt. of Ophthalmology Vinohrady Teaching Hospital Prague, Czech Republic

All authors have No finacial interest

Aim of the study

• To evaluate quality of Pre-Cut corneas for DSAEK prepared in the Interantional Eye Bank of Prague

• To analyse possible complications during preparation of the Pre-Cut corneas.

• To analyse possible complications after preparation of Pre-Cut corneas

Methods - preparation

• Pre-Cut corneas were prepared from 30 corneas – not suitable for surgery

• All Pre-Cut corneas were preapred in „Clean rooms“ by certified staff members

• All Pre-Cut corneas were prepared using standardized method for DSAEK – with Moria microkeratome

Ljfpqejqoj tpojJkpavjpjp ojp

Moria microkeratome

No major technical problems during preparation

Mounting

Epithelial abrasion

Pachymetry

Free flap reposition

Free flap evaluatin

Cut

Dismounting

Methods - Evaluation

• All Pre-Cut corneas were evaluated using– Slit lamp– Specular microscopy

• Selected Pre-Cut corneas were screend for – Microbial comtamination

Ljfpqejqoj tpojJkpavjpjp ojp

Results - Slit lamp evaluation

• All 30 Pre-Cut corneas were evaluated using slit lamp• In 28 Pre-Cut corneas we found good position of the corneal

free flap• In 2 cases we foud dislocation of the free flap into the

medium• No obvious stromal or endotehelial defects or opacity were

detected

Ljfpqejqoj tpojJkpavjpjp ojp

Incorrect position of

the free flap

Correct position of

the free flap

Results Specular microscopy evaluation

• The average endothelial cell censity prior PreCut was 2709±483(SD) cells/mm2,

• The average endothelial cell censity after PreCut was 2623±407(SD) cells/mm2

• We found no significant endothelial cell loss after preparation.

Corneal endothelium after Pre-Cut

Endothelial Cell Density

Ljfpqejqoj tpojJkpavjpjp ojp

Resuts - Microbiology

• 5 randomly selected samples of medium and corneal swaps were microbiologically tested for microbial and fungal contamination – all results were negative

• no medium showed signs of bacterial contamination 1 week after preparation of the Pre-Cuts.

Summary

• Preparation – In our serie we experienced no major technical

problems– The procedure is straight forward with relatively

short learning curve

• Evaluation– In our serie evaluation of prepared tissue showed

high quality and high safety

Conclusion

• We believe that our eye bank prepares Pre-Cut corneas for lamelar keratoplasty of very high quality and safety

• We also believe that in the near future Pre-Cut corneas will represent majority of tissues provided by our eye bank

Thank you Thank you

International Eye Bank of PragueSrobarova 50100 34 Prague 10 Czech Republice-mail:eyebank@fnkv.czTel: +420 26716 3678, +420 26716 2267