THE COMPOUND LIGHT MICROSCOPE

USING THE COMPOUND LIGHT MICROSCOPE

• WET MOUNT REVIEW

• MOVING THE SLIDE

• LETTER INVERSION

• DEPTH OF FIELD

• MAGNIFICATION

EYEPIECE (10x) x LENS (10X LOW OR 40X HIGH)=100X, 400X

USING THE COMPOUND LIGHT MICROSCOPE• MEASUREMENT IN MICROMETERS ( µ )

1mm = 1000 µm and .001mm = 1 µm

• DIAMETER OF FIELD

EXAMPLE UNDER HIGH POWERDIAMETER (LOW POWER)= 4 mm

DIAMETER (HIGH POWER)

40/10 = 4 4000/4 = 1000 µm

• PROPER CARE AND USAGE

MICROSCOPE LAB DIRECTIONS SUMMARYTEXTBOOK PAGES 691-694

• NOTES: SEE MATERIAL LIST ON P.691

• SKIP STEP #’S 19-24

• REVIEW MICROSCOPE DIAGRAM ON THE TOP LEFT OF P.692 FOR PARTS A AND B

• YOU DO NOT HAVE TO DO TABLE PC.1

• FOR PART C, MAKE WET MOUNT SLIDES OF THE LETTERS O, C, AND E AND DRAW WHAT YOU OBSERVE UNDER LOW OR HIGH POWER (10X OR 40X). KEEP YOUR WET MOUNT OF THE LETTER “E” FOR #33.

• NOTICE WHAT HAPPENS TO YOUR SPECIMEN WHEN YOU MOVE THE SLIDE ONE WAY OR THE OTHER.

• PAY ATTENTION TO WHAT HAPPENS TO YOUR FIELD OF VIEW WHEN YOU INCREASE MAGNIFICATION (AS WELL AS IF YOU HAVE TO ADJUST YOUR LIGHT DIAPHRAGM).

• FOR PART D, USE THE COLORED THREADS SLIDE IN THE BACK OF THE ROOM FOR #25-30.

• FOR PART E, USE THE TRANSPARENT RULER IN THE BACK OF THE ROOM TO MEASURE YOUR FIELD OF VIEW USING THE 4X OBJECTIVE LENS (USE mm MARKINGS AND PLACE THE RULER ON THE FAR LEFT OF YOUR FIELD OF VIEW, MAKE YOUR MEASUREMENT AND THEN CONVERT IT TO MICROMETERS).

• FINISH THE LAB BY DOING ANALYSIS QUESTIONS #1-6 ON P.694.

HOW TO USE THE MICROSCOPE

ANALYSIS QUESTIONS P.694

1. MICROSCOPE MORE DETAILED, LARGER IMAGES, THE OBJECT MOVES IN THE OPPOSITE DIRECTION OF HOW YOU MOVE THE SLIDE, OBJECTS ARE INVERTED AND REVERSED

2. AS MAGNIFICATION INCREASES RESOLUTION DECREASES (USE THE FINE FOCUS ADJUSTMENT)…AS FIELD OF VIEW DECREASES, FIELD OF VIEW IS DARKER (ADJUST DIAPHRAGM FOR LIGHT)…TO FOCUS WITHIN DEPTH OF FIELD (USE THE FINE FOCUS ADJUSTMENT)

3. RED ON TOP, BLUE IN THE MIDDLE, YELLOW ON BOTTOM…DEPTH OF FIELDUSE FINE FOCUS ADJUSTMENT (WHEN ONE THREAD IS FOCUSED AND DETAILED, THE OTHERS ARE NOT)

4. AS MAGNIFICATION INCREASES, THE DIAMETER OF THE FIELD OF VIEW DECREASES

5. 4 mm = 4000 µm

6. 40/10 = 4, 4000/4 = 1000 µm

MICROSCOPE QUIZ FORMAT

• 21 M.C. QUESTIONS

• 3 M.C. BONUS QUESTIONS (1 PT. EACH)

• REVIEW: OUTLINE SHEET, LAB ANALYSIS QUESTIONS, STUDY GUIDE QUESTIONS, MICROSCOPE DIAGRAM p.692

MICROSCOPE STUDY GUIDE

1. A

2. C

3. B

4. A

5. B

6. C

7. C

8. B

9. B

10. D

11. D

12. D

13. A

14. A

15. B

16. A

17. A

18. A

19. C

20. B