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The Citric Acid Cycle Mary Gearing

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Page 1: The Citric Acid Cycle Mary Gearing. Problems with this pathway Where does the oxaloacetate come from? How do we convert oxaloacetate to malate?

The Citric Acid Cycle

Mary Gearing

Page 2: The Citric Acid Cycle Mary Gearing. Problems with this pathway Where does the oxaloacetate come from? How do we convert oxaloacetate to malate?
Page 3: The Citric Acid Cycle Mary Gearing. Problems with this pathway Where does the oxaloacetate come from? How do we convert oxaloacetate to malate?

Problems with this pathway

• Where does the oxaloacetate come from?

• How do we convert oxaloacetate to malate?

Page 4: The Citric Acid Cycle Mary Gearing. Problems with this pathway Where does the oxaloacetate come from? How do we convert oxaloacetate to malate?

Where in the world is 1.1.1.37?

• BLAST our genome against 1.1.1.37 (H. salinarium)

Page 5: The Citric Acid Cycle Mary Gearing. Problems with this pathway Where does the oxaloacetate come from? How do we convert oxaloacetate to malate?

Mistaken identity???

Page 6: The Citric Acid Cycle Mary Gearing. Problems with this pathway Where does the oxaloacetate come from? How do we convert oxaloacetate to malate?

Different BLAST result

Page 7: The Citric Acid Cycle Mary Gearing. Problems with this pathway Where does the oxaloacetate come from? How do we convert oxaloacetate to malate?
Page 8: The Citric Acid Cycle Mary Gearing. Problems with this pathway Where does the oxaloacetate come from? How do we convert oxaloacetate to malate?
Page 9: The Citric Acid Cycle Mary Gearing. Problems with this pathway Where does the oxaloacetate come from? How do we convert oxaloacetate to malate?

Can our organism convert pyruvate to oxaloacetate? (Enzyme 6.4.1.1)

PyruvatePyruvate

OxaloacetateOxaloacetate

6.4.1.16.4.1.1

Page 10: The Citric Acid Cycle Mary Gearing. Problems with this pathway Where does the oxaloacetate come from? How do we convert oxaloacetate to malate?

BLASTing our genome

Page 11: The Citric Acid Cycle Mary Gearing. Problems with this pathway Where does the oxaloacetate come from? How do we convert oxaloacetate to malate?

Both correspond to EC number 2.3.3.13 (2-isopropylmalate synthase)

Page 12: The Citric Acid Cycle Mary Gearing. Problems with this pathway Where does the oxaloacetate come from? How do we convert oxaloacetate to malate?
Page 13: The Citric Acid Cycle Mary Gearing. Problems with this pathway Where does the oxaloacetate come from? How do we convert oxaloacetate to malate?

So where does the oxaloacetate come from anyway?

• Aspartate metabolism

• Glycoxylate metabolism

L-aspartateL-aspartate OxaloacetateOxaloacetate2.6.1.12.6.1.1

Page 14: The Citric Acid Cycle Mary Gearing. Problems with this pathway Where does the oxaloacetate come from? How do we convert oxaloacetate to malate?

Unanswered Questions

Oxaloacetate/malate conversion

Pyruvate/oxaloacetate conversion

We still don’t know if these processes occur in H. utahensis.


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