THE JOURNAL OF IMMUNOLOGY

Test tubes to answers. The complete radioassay systems.

Searle Analytic (formerly Nuclear-Chicago) offers you the only complete on-line radioimmunoassay/

competitive protein binding (RIA/CPB) systems. Systems that automate the entire radioassay

procedure--from analyzing RIA/CPB samples to printing out immediate, meaningful results. You only

load standards and samples, establish assay protocol, start the system, and retrieve final, hard

copy answers. Our systems do all the rest!

Assay preparation isn't changed at all. Yet answers are transformed directly to averaged count rate,

normalized percent bound, standard deviation, dose, corrected dose, and confidence range for each

sample group. It's all performed by our RIA/CPB Data Processor, which can be linked to either our beta or

gamma spectrometer systems. The combination provides unprecedented speed and convenience in data-reduction.

But we didn't stop with immediate answers in RIA. For the wide variety of kits now in commercial use, our spectrometer systems let you program and count many combinations of tests in the same run. Or, with our exclusive SRA 2 T~ System, simultaneously operate both beta and gamma systems from a single RIA/CPB Data Processor.

Whatever the demand--raw RIA/CPB data, spec- trometer systems for any use, or the right systems for your particular lab--we provide complete answers. You'll find your questions answered in our free brochure, RtA/CPB Data Systems. Write to us today.

SEARLE

Searle Analytic Inc. (Formerly Nuclear-Chicago) Subsidiary of G. D. Searle & Co. 2000 NucJear Drive Des Plaines, tlHnois 60018

Searle Sales and Service Offices in Major Cities World Wide ALS'403

A I A . - - . 4 = , ~ =,U,*

Bionetics Laboratory Products is actively involved in bringing new and useful reagents to the immunology and virology laboratory, Other products currently available or soon to be announced include: • Mouse Myeloma Proteins- Immunoglobulins and Free Light Chains • Antisera to Mouse Myeloma Proteins • Purified RNA Tumor Viruses and their Components • Antisera to RNA Tumor Viruses and their Components • EBV Antigen Slides (VCA, EA, and EBNA)

Please direct inquiries to Mr. Richard D. Eisenberg. BIONETICS

Laboratory Products Litton

5510 Nicholson Lane Kensington, Maryland 20795 Telephone: (301) 881-5600

ETHENO NUCLEOSIDES and NUCLEOTIDES A new too l fo r enzymologis ts and cytologists.

Extremely high fluorescence permits easy detection at concentrations as low as I0 "g M.

The potent ia l ut i l . i ty o f these h igh ly f luorescent compounds as a probe in enzymic mechanism and structure is indicated by the i r long f luorescent l i fe t ime (close to 20 nsec), de tec tab i l i t y at l ow concen- t ra t ion and re lat ive ly long wavelength o f exc i ta t ion . Science 177:279, 1972, and Science 175:646, 1972

MANY NOVEL APPLICATIONS ARE POSSIBLE:

• 1•N6•Ethen•aden•sine•3•:5••M•n•phosphatehasbeenfoundt•beahigh•yacceptabIesubstituteforAden•sine•3+:5 +- Monophosphate in protein kinase systems. Science 177:279, 1972

* 1,Ng-Ethenoadenosine-5'-Triphosphate is reported to show considerable substrate activity as a replacement tor Adenosine-5'-Triphosphate with adenylate kinase, hexokinase and phosphofructokinase. Biochemistry 11:3499, 1972

• 1,Ng*Ethenoadenosine-5'-Diphosphate has proved to be an excellent substitute for Adenosine-5'-Diphosphate in the pyruvate kinase system, affording a facile assay for a wide variety of kinases. Biochemistry 11:3499, 1972

• Nicotinamide-l,Ng-Ethenoadenine Dinucleotide, a fluorescent analog of Nicotinamide Adenine Dinucleotide, is reported to show reasonable substrate activity in the Yeast or Horse-Liver Alcohol Dehydrogenase, Glyceraldehyde- 3-Phosphate Dehydrogenase and Lactic Acid Dehydrogenase systems. Proc Nat Acad Sci 69:2039, 1972

= Nicotinamide-1 ,N 6-Ethenoadenine Dinucleotide Phosphate, a fluorescent analog of Nicotinamide Adenine Dinucleo- tide Phosphate, shows considerable substrate activity with Glucose-6-Phosphate Dehydrogenase, but is essentially inactive with lsocitric Dehydrogenase from pig heart. Sigma Chemical Company, unpublished

- - o ' ' • • - -

These Etheno compounds are already available from Sigma:

1,Ng-ETHENOADENOSINE 2rag $ 7.00 5'-DIPHOSPHATE

3,N+-ETHENOCYTIDINE Img $ 6.50 E 3378 tOmg 19.80 E 3753 5"-TRIPHOSPHATE lOmg 36.00

Sodium salt, Approx, 98% tOOmg 134.50 Sodium salt. Approx. 98% lOOmg 200.00

3,N4-ETHENOCYTIDINE l mg 4.00 I'Ng"ETH EN 0"2" 17rig 6.50 E 3505 5'-MON OPHOSPHATE lOmg 2 2 . 5 0 DEOXYADENOSlNE lOre# 36.00

Sodium salt. Approx. 98% lOOmg 125.00 E 4003 5"-MONOPHOSPHORIC ACID lOOmg 200.00 Sodium salt. Approx. 98%

j 1,N +-ETH ENO-2"- 3,N4-ETHENOCYTIDINE I mg 6 . 5 0 DEOXYADENOSINE 1 mg 6.50

E 3628 5'-DIPHOSPHATE lOmg 36.00 lOmg 36.00 Sodium salt. lOOmg 200.00 E 4128 5'-TRIPHOSPHATE Sodium salt. lOO mg 200.00

N 3630 NICOTINAMIDE 1,Ng-ETHENOADENINE DINUCLEOTIDE PHOSPHATE 1 mg$ 12+00 10 mg 65. O0 Grade II, Sodium salt. Approx. 90% t00 mg 360.00

Previously announced Ethenos:

E 2378 1,N6=ETHENOADENOSINE E 3003 I,Ng-ETHENOADENOSINE-5'-TRIPHOSPHATE E 2253 1,Ng-ETHENOADENOSINE-3':5"-CYCLIC E 2878 3,N4-ETHENOCYTIDINE HYDROCHLORIDE

MONOPHOSPHORIC ACID N 2630 NICOTINAMIDE-1,N6-ETHENOADENINE E 9262 1,Ng-ETHENOADENOSINE HYDROCHLORIDE DINUCLEOTIDE E 9127 I,Ng-ETHENOADENOSINE-5'-MONOPHOSPHORIC ACID

C.I.F. Destination Anywhere in the World Via Air.

4174

If you need other etheno compounds, please write or phone us.

ORDER DIRECT

TELEPHONE COLLECT ~ from ANYWHERE in the WORLD

Station to Station, 314-771-5750 (Including Saturday and Sunday until 1 P.M.)

- O - Night, Person to Person,

Dan Broido, 314~993-6418

Most orders will be shipped within hours TWX [Teletype],Oay or N~ght: COLLECT 910-761-0593

TELEGRAM: SIGMACHEM, Saint Louis, Missouri

I I/N ,,e ,..,,g, ,..,.,o+g.,

d,v,sion of SIGMA INTERNAIIONAL, LTD

P.O. BOX 1 4 5 0 8 • SAINT LOUIS, MISSOURI 6 3 1 7 8 U.S.A.

Distributed through: SIGMA LONDON Chem. Co. Ltd • NorbJton Station Yard, Kingston-upon Thames, Surrey. KT2 7BH, England

Telephone: 01-549 3171 (Reverse Charges)

SIGMA CHEMIE Gmbh MI)NCHEN • D 8014 INeub,berg bet Munchen. Isarstrasse 14. W Germany Telephone 089 60 70 03 (Reverse Char~esl

N Y E 6 A A R D & CO.

SODIUM METRIZOATE SOLUTION

(ISOPAQUE)

AND

LYMPHOPREP SODIUM METRIZOATE*/FICOLL® SOLUTION

For preparation of pure lymphocyte suspensions

Blood

Separation fluid

Before After

Before and after centrifugation

Lymphocytes

R eferences

Boyum, A. (1964): Separation of white blood cells. Nature 204, 793.

Boyum, A (1968): Separation of leucocytes from blood and bone marrow. Scand. J. Clin. Lab. Invest. 21, Suppl. 97.

Favour, C, B. (1964): Antigen-antibody reactions in tissue culture. Immunological Methods, ed. J. R. Ackroyd, pp. 195-223, Blackwell Scientific Publ., Oxford.

Thorsby, E. & Bratlie, A. (1970). A rapid method for preparation of pure lymphocyte suspen- sions. Histocompatibility Testing t970, ed. P.I. Terasaki, p. 655. Munksgaard, Copenhagen.

A T LAST/

Available in the U.S.A.!

GALLARD-SCHLESINGER CHEMICAL MFG. CORP., 582 MINEOLA AVE, CARLE PLACE

LI., N.Y. 11514 6 (516) 333-5600

Gallard/Sclllesinger

THE JOURNAL OF I M M U N O L O G Y

M E L O Y . . . Most Complete Line Of

Immunochemical Products AVAILABLE FOR IMMEDIATE DELIVERY

9WELL* 24WELL* Consult Meloy catolog for quantity pricing KITS KITS of all Meloy products.

(54detns.) (72 detns.) IgG, IgM, IgA, IgD, Albumin, Alpha I Antitrypsin, Ceruloplosmin, $48.60 $60.48 Alpha: Macroglobulin, Transferrin, C'3, C'4 IgE 52.00 62.00 Low Level IgG, IgA, IgM 5%00 72.74

IgG, IgM, IgA, IgD, Albumin, Alpha 1 Antitrypsin, Ceruloplasmin Alpha2 Macroglobulin, Tronsferrin C'3, C'4 $15.00/2 ml.

Bence-Jones Kappa, IgE 20.00/2 ml.

Bence-Jones Lambda Polyvalent Immunoglobulins 24.00/2 ml.

Whole Human Serum 12.00/2 ml.

PLATES KIT PACKAGE

AGM PLATES [72detns.] $73.44 $60.94

ALSO AVAILABLE Fluorescein Conjugated Antisera (human and animal), Mouse Immunoglobulin Plates, Abnormal Reference Sera, Radioimmunoassoy, ANA, C'5, etc. Catalog available upon request.

ANTISERA

AGM PLATE

The Immunology People E L t ~ BIOLOGICAL PRODUCTS DIVISION

6 7 1 5 ELECTRONIC DRIVE, SPRINGFIELD, V I R G I N I A 2 2 1 5 1

ON MAY i, 1974 HYL AND'S NEW IMMUNO-PI TE ]]1][ OBSOLETED EVERY OTHER RADIAL IMMUNODIFFUSION SYSTEh AROUN

NOW YOU CAN PROVIDE IgG, lgA AND IgM END-POINT PROFILES IN 48 HOURS FLAT.

H y l a n d ' s new Imrnuno-P la te I I I uti l izes the precision end-poin t t echnic to give you r e su l t s t h a t r each comple t ion in only 48 hours . I t ' s the f a s t e s t IgG, IgA and IgM end-poin t proi~fle d e t e r m i n a t i o n avai lab le f rom any m a j o r m a n u f a c t u r e r .

OR MEASURED-TIME DETERMINATIONS AS QUICKLY AS 4 HOURS.

Utilizing the star technic, you can have r e su l t s in 4 hours . Or 16-18 hou r s utilizing the overnight technic. The choice is yours . And you d o n ' t have to swi tch f rom one p la te to another . T h e new I m m u n o - P l a t e I I I offers all t h r e e opt ions (4 h o u r stat , 16-18 h o u r overn igh t or 48 h o u r precis ion) wi th the s a m e plate.

WITHOUT SERUM DILUTION. With all m a j o r tes ts , s e r u m di lu t ions a re

not r equ i red wi th in no rma l adu l t work ing ranges. You save t ime. You r educe the r i sk of p r o c e d u r e error .

WITHOUT OTHER INCONVENIENCES. Clear prec ip i t in zones, w i t h o u t staining,

a re the ru le wi th the new I m m u n o - P l a t e III . A n d to m a k e the p r o c e d u r e eas ie r still, each p la te is c lear ly labeled, each well is ser ia l ly n u m b e r e d .

AND WITH A WHOLE LOT MORE. T h e new H y l a n d I m m u n o - P l a t e I I I s y s t e m e n c o m p a s s e s a wide r ange

of s e r u m p ro t e in tes ts . Each t e s t offers t he s a m e 4, 16-18 and 48 h o u r flexibility. Each inco rpora t e s the m a n y i m p r o v e d fea tures . But t h e r e ' s even m o r e you ' l l be i m p r e s s e d by wi th th is advanced new radia l immunod i f fus ion sys t em. Th ings

~ducibili ty and l ineari ty. W e ' r e su re you ' l l w a n t to h e a r ~y not call y o u r H y l a n d r e p r e s e n t a t i v e today?

: ;a l ::,F-."~e_ ~ B : I : ' E;Sz- [ : :£~. = r A iBs* .& : :a , . i : i3 : r~&

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THE JOURNAL OF IMMUNOLOGY

not l~y chance. Durable plastic chambers at- tached to a glass slide by a removable gasket simplify work. No tubes. No dishes. No need to transfer cells. Avoids dangers of contamination. The start-to-st6rage system assures you of a readable permanent record because it's on the slide. Lab-Tek's good manufacturing procedures insure reproducible results. Lab-Tek ® Tissue Culture CHAMBER/SLIDE is useful for growing clones, cell lines, and strains. We have many reports of how useful Tissue Culture CHAMBER/SLIDES are for ANA and other FA tech- niques but if by chance you've adapted our CHAM- BER/SLIDE to some other useful technique, please let us know. Lab-Tek Tissue Culture CHAMBER/SLIDES, #4808 {8- chamber}, are available from your Lab-Tek Products Service-Certified Distributor.

L,3_ b-Tek Products D,vision Miles Labo,atories. Inc. 30W475 N Auro,;z Rd. Napmvil le. Il l inois 60540

Lab-Tek P roduc t s~ . . . se t t ing the s t andards by which performance is judged

We have the best medical dictionary

STEDMAN'S 22 That may sound like a big claimmbut we believe you'll agree with us when you use the brand new 22nd edition of Stedman's Medical Dictionary.

And we know you will want to use it, because it is the most authoritative, most comprehensive, and most up-to-date reference book the physician, medical student or researcher can buy. That's why we've invested seven years in re- vising, expanding, redesigning and rethinking our good old dictionary. Thirty- four experts have worked on the forty-four specialties for an average of three years apiece. (Where else can you find an accumulated 102 years of current medical knowledge?) And to assist these human brains, we enlisted the services of a computer for overall editorial review. (Where else does a great past unite with today's technology?) We think it's about time the medical profession had the best. So we're offering you our investment. Your investment in Stedman's 22 will pay off. We can promise you that. So order your copy today. You'll never regret it.

The facts about Stedman's 22 Our new dictionary contains 7,199 significant new vocabulary entries reflecting new information from 44 specialties and subspecialties in the life sciences; 24,877 important editorial revisions presenting new information on 30 r/r, of the vocabu- lary; excellent color plates and illustrations presenting graphic ac- companiment for hundreds of vo- cabulary entries; an alphabetical

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PAP

Pe roxidase-A nti-Peroxidase

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lml Concentrate

Produced in:

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Specialists in Fluorescein, Rhodamine, Ferritin, Peroxidase and Custom Conjugations.

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