test report n. eg 180406-05 · 2020. 4. 28. · uni en iso 16911-1 . solide a srl - p.iva e cf...

Sampling of: ambient air taken during the operation of the purifying machine AERIA 400 Performed by: our operators on 06.04.2018 (9:00 -11 :30) Place of sampling: MUSP compressor room (Piacenza site) Purpose of the survey : calculation of the purification efficiency of ultrathin powders (PM 2.5) aero-dispersed in indoor environment by AERIA 400 Description of sampling point: center of the compressor room Room volume : approximately 25 m3 External environmental microclimatic data: Average tempe-rature: 14.2 °c -Average relative humidity: 88.5 % U.R.

TEST PROCEDURE METHOD 1st PHASE (measurement of fluid-dynamic parameters)

Before the test is performed, the fluid-dynamic parameters of AERIA 400 were measured in the condition of maximum operating potential. The results can be summarized as follows:

2nd PHASE (preparation of forced polluted atmosphere)

In the room used for the tests, a forced contaminated atmo-sphere was created through the combustion of a standardi-zed quantity of vegetable charcoal (15 grams) blended with paraffin tablets (10 grams); the combustion phase was extended for a period of eight (8) minutes. In the room, had been positioned the equipment for the air sampling with a selector capable of differentiating the aero-dispersed powders, according to their diameter, in the following three fractions:

- PM > 1 O micron - PM between 10 e 2.5 micron- PM < 2.5 micron

In these environmental conditions, our operators have sampled the suspended powders by a cellulose nitrate membrane; subsequently, the quantitative determination of the powders was performed in the laboratory by gravimetric way. The result obtained was taken as a reference to calculate the abatement efficiency of AERIA 400.

3rd PHASE (operation of AERIA 400 at maximum potential)

After ventilation of the room until complete removal of powders, the same conditions of forced environmental contamination were recreated as described in 2 nd phase. AERIA 400 has been turned on to its maximum operating potential, for a total time of sixty (60) minutes. In this way, it is reasonable to assume that around eight (8)complete air changes have been carried out in the room.At the end of the machine operation, the sampling of residual powders was repeated in the same modalities described in 2 nd phase.

TEST REPORT n. EG 180406-05 1/2

Parameters determined

Average temperature of the emission Average temperature of the emission

Conduit section (circular)

Emission speed Hourly flow of the emission

Normalized hourly flow of the emission

Unit ofmeasure

°CK

M2

m/sm3/h

Nm3/h

Values measured atmaximum potential

14.2 287.2

0.0283

2.05209199

Test method

UNI EN ISO 16911-1

SOLIDEA SRLwww.solidea-srl.it - P.Iva e CF 03701880365 - Via Pirandello 31 - 41051 Castelnuovo Rangone (Mo) Italy


Sampling of: ambient air taken during the operation of the purifying machine AERIA 400 Performed by: our operators on 06.04.2018 (9:00 - 11 :30) Place of sampling: MUSP compressor room (Piacenza site) Purpose of the survey : calculation of the purification efficiency of ultrathin powders (PM 2.5) aero-dispersed in indoor environment by AERIA 400 Description of sampling point: center of the compressor room Room volume : approximately 25 m3 External environmental microclimatic data: Average temperature: 14.2 °C - Average relative humidity: 88.5 % U.R

NOTES ABOUT THE AIR-SAMPLING

RESULT OF TEST

COMMENTS and INTERPRETATION

With regard to the way the test was conducted, it was possible to verify that in the monitored room, the operation of AERIA 400 reduced the concentration of PM2.5 powders by 82.6% This purification efficiency was achieved using AERIA 400 to its maximum potential, so as to achieve eight complete air changes in the room, in a time of sixty minutes.



Average temperature of the air in the environment Average temperature of the air in the environment

Average relative humidity of the ambient air

Total volume of sampled air: 928 L c.n. Total number of air changes by AERIA 400: eight

Unit ofmeasure

°CK

% U.R.

Values at the startof the test

15.8 288.865.2

Values at the endof the test

15.5 288.568.5

Measurement conditions

Measurement performed before activation of AERIA 400

Measurement performed after operation of AERIA 400 for a period of 60 minutes at

maximum potential

Unit ofmeasure

µg/Nm3

µg/Nm3

Ultra-thin powders (PM 2.5)

1580

275

Test method

UNI 13284-1


Sampling of: ambient air taken during the operation of the purifying machine AERIA 400 Performed by: our operators on 06.04.2018 (12:00 - 15:00) Place of sampling: MUSP compressor room (Piacenza site) Purpose of the survey : calculation of the purification efficiency of ultrathin powders (PM 2.5) aero-dispersed in indoor environment by AERIA 400 Description of sampling point: center of the compressor room Room volume : approximately 25 m3 External environmental microclimatic data: Average tempe-rature: 15.5 °C - Average relative humidity: 81.8 % U. R.

TEST PROCEDURE METHOD 1st PHASE (measurement of fluid-dynamic parameters)Before the test is performed, the fluid-dynamic para-meters of AERIA 400 were measured in the condition of minimum operating potential. The results can be summarized as follows:

2nd PHASE (preparation of forced polluted atmosphere)

In the room used for the tests, a forced contaminated atmo-sphere was created through the combustion of a standardi-zed quantity of vegetable charcoal (15 grams) blended with paraffin tablets (10 grams); the combustion phase was extended for a period of eight (8) minutes. In the room, had been positioned the equipment for the air sampling with a selector capable of differentiating the aero-dispersed powders, according to their diameter, in the following three fractions:

- PM > 1 O micron - PM between 10 e 2.5 micron- PM < 2.5 micron

In these environmental conditions, our operators have sampled the suspended powders by a cellulose nitrate membrane; subsequently, the quantitative determination of the powders was performed in the laboratory by gravimetric way. The result obtained was taken as a reference to calculate the purification efficiency of AERIA 400.

3rd PHASE (operation of AERIA 400 at maximum potential)

After ventilation of the room until complete removal of powders, the same conditions of forced environmental contamination were recreated as described in 2 nd phase. AERIA 400 has been turned on to its minimum operating potential, for a total time of sixty (60) minutes. In this way, it is reasonable to assume that around six (6) complete air changes have been carried out in the room.At the end of the machine operation, the sampling of residual powders was repeated in the same modalitiesdescribed in 2 nd phase.



Average temperature of the emission Average temperature of the emission

Conduit section (circular)

Emission speed Hourly flow of the emission

Normalized hourly flow of the emission

Unit ofmeasure

°CK

M2

m/sm3/h

Nm3/h

Values measured atmaximum potential

14.9 287.9

0.0283

1.55158150

Test method

UNI EN ISO 16911-1


Sampling of: ambient air taken during the operation of the purifying machine AERIA 400 Performed by: our operators on 06.04.2018 (12:00 -15:00) Place of sampling: MUSP compressor room (Piacenza site) Purpose of the survey: calculation of the purification efficiency of ultrathin powders (PM 2.5) aero-dispersed in indoor environment by AERIA 400 Description of sampling point: center of the compressor room Room volume : approximately 25 m3 External environmental microclimatic data: Average temperature: 15.5 °C -Average relative humidity: 81.8 % U.R.

NOTES ABOUT THE AIR-SAMPLING

RESULT OF TEST

COMMENTS and INTERPRETATION

With regard to the way the test was conducted, it was possible to verify that in the monitored room, the operation of AERIA 400 reduced the concentration of PM2.5 powders by 90.2 % This purification efficiency was achieved using AERIA 400 to its minimum potential, so as to achieve six complete air changes in the room, in a time of sixty minutes.



Average temperature of the air in the environment Average temperature of the air in the environment

Average relative humidity of the ambient air

Total volume of sampled air: 936 L c.n. Total number of air changes by AERIA 400: six

Unit ofmeasure

°CK

% U.R.

Values at the startof the test

16.2 289.267.8

Values at the endof the test

15.8 288.868.8

Measurement conditions

Measurement performed before activation of AERIA 400

Measurement performed after operation of AERIA 400 for a period of 60 minutes at

maximum potential

Unit ofmeasure

µg/Nm3

µg/Nm3

Ultra-thin powders (PM 2.5)

1580

155

Test method

UNI 13284-1


This Report shows the results of the research and development and the operational test of an organic-based sanitizing agent for the microbiological control of the water tank of the AERIA 400 air purifying machine, as per the Research and Confidential Assignment Agreement signed by both sides on 3/11/2017. It has been developed a sanitizing agent with a biocide and biostatic effect based on medium-short chain fatty acids and their esters. This mixture was introduced into the AERIA 400 prototype machine, and it was placed in an environment whose characteristics are: T°: 23-25°C RH: 44,1 Volume: 60 m2; 200 m3 Presence of 4 people at work who came out and entered in the room (at least 10 times) Subsequently, three areas of interest were sampled: 1. Total Microbiological Charge of the water;2. Total Microbiological Charge of the air of the machine;3. Total Microbiological Charge of the environment described above.

The samplings were performed with these times: • T0 -> 1 minute after starting the machine;• T1 -> 1 hour after starting the machine;• T3 -> 3 hours after starting the machine;• T4 -> 4 hours after starting the machine;• T5 -> 5 hours after starting the machine;• T24 -> 24 hours after starting the machine.

The methods used for the analyses are: • For water: protocol ISO 6222: 1999;• For air and environment with the SAS system (surface air system), volume withdrawn 200 m3.

The plates were incubated for 24 hours at 30 ° C (after 48 and 72 hours the plate counting was repeated to verify that no late growths were present). The growth medium was the Plate Count Agar. In addition to the sanitizing compound, commercial sanitizer was supplied with the same parameters and protocols provided directly by the client, the concentration used was 2% w / w.

Microbiological results Microbial loads are expressed in:• UFC/ml for the water;• UFC/m3 for the air and the environment.

pg.01/02

Report on the activity of Research andDevelopment of an organic sanitizer forthe microbiological control of water in theAERIA 400 air purification plant.

Commercial Sanitizer 2% Time (ore)Water AirEnvironment

010034

160

120042

116

33004032

41363

6688

54000100116

2431621324112

Sanificante Packtin 0.75% Time (ore)Water AirEnvironment

010013

176

11001117

3100

718

41702068

5800

914

241000

1520


pg.02/02

1

10

100

1000

10000

100000

0 5 10 15 20 25 30

UFC

/*

ore

Commercial Sanitizer 2%

Acqua Aria Ambiente

1

10

100

1000

0 5 10 15 20 25 30

UFC

/*

ore

Packtin Sanitizer 0.75%

Acqua Aria Ambiente

1

10

100

1000

10000

100000

0 5 10 15 20 25 30

UFC

/ml

ore

Water

commercial sani�zer 2% sanificante Pack�n 0.75%

1

10

100

1000

0 5 10 15 20 25 30

UFC

/m3

ore

Air

1

10

100

1000

0 5 10 15 20 25 30

UFC

/m3

ore

Environment

As the graphs show, the greater effectiveness of the "Sanitizing Packtin" product on all three areas of interest is evident. Moreover, the first graph shows that the microbial charge of water is related to both the one of the air and the one of the environmental. After 8 hours, the water charge in the "commercial sanitizer" tests is sufficiently contaminated to raise the microbiological charge in the air; therefore, it will affect the environmental one. This behavior is not observed in the Packtin sanitizer. Furthermore, during the tests the water loss parameter was evaluated. The results demonstrated a loss of: 8.8 L after 24 hours of operation (loss 360 ml / h); 9.1 L after 27 hours of operation (loss 340 ml / h). The Microbiological analyzes were carried out by Dr. Riccardo De Leo and validated by Professor Andrea Pulvirenti of the University of Modena and Reggio Emilia.




This Report shows the results of the research, development and the operational test of an organic-based sanitizing agent for the microbiological control of the water tank of the AERIA 400 air purifying machine, as per the Research and Confidential Assignment Agreement signed by both sides on 3/11/2017. It has been developed a sanitizing agent with a biocide and biosta-tic effect based on medium-short chain fatty acids and their esters. Concentration 0.75% w/w The following report (2 of 3) describes the antimicrobial activity of the sanitizer against four food pathogens bacteria The method used is a Kirby-Bouer Diffusion test in plate:

• Inoculum size: 100 microliters of physiological solution with microbial load of 1x107 CFU / ml.• Paper disk dimensions: 5 mm• Incubation for 24 h at 30 ° C

pg.01/02

Activity againstCampylobacter jejuni

Activity againstEscherichia coli

Activity againstListeria monocytogenes

Activity againstSalmonella Typhimurium

Report on the activity of Research andDevelopment of an organic sanitizer for themicrobiological control of water in theAERIA 400 air purification plant.


pg.02/02

Packtin sanitizer at the concentration of 0.75% shows an inhibition against the microorganisms involved in the study. The best activity is against Gram + bacteria (Campylobacter and Listeria). The best result is observed against Listeria monocytogenes with an inhibition area of 12 mm. Dr. Riccardo De Leo,

Microorganism

Campylobacter jejuni

Escherichia coli

Listeria Monocytogenes

Salmonella typhimurium

Inhibition zone dimension (mm)

9.7

9.2

12.0

7.9


AUTHORIZED EXCERPT WITH A SUMMARY OF THE MAIN EVIDENCE

Customer : SOLIDEA S.r.l.

Analysed material : Exhaust gas residues Responsible: Dr. Antonietta Gatti

Signed:

Date: 02 March, 2015 rev. 20/10/2017

Note: the present document is an authorized excerpt from the document created on March 02, 2015 by Nanodiagnostics srl. which includes the full results of the analysis.

INTRODUCTIONNanodiagnostics srl is a scientific consultancy firm in the fields of medicine, industry and ecology. The main activity is the study of organic and inor-ganic samples by means of environmental electron microscopy with the aim of detecting micro- and nano-sized inorganic particles. The survey mainly uses a field-emission environmental scanning electron microscope (Quanta 200, FEI Company, The Netherlands). In the present case, it was required to analyse particles trapped by a water filtration system applied for about half an hour to the exhaust pipe of a powered tractor. The test had as its object the analysis of the liquid contained in the upper part of the reservoir of the potting liquid filtered by the exhaust of the trac-tor. The analysis verified the nature of the parti-culate matter present in the repository by means of scanning electron microscopy (FEG - ESEM Quanta 200, FEI), while X-ray microanalysis (EDS) evaluated the elemental chemical composition. No processing was performed on the sample which was analyzed as it was. The method allows to evaluate the presence of particles of micro- and nanometric size.

ANALYSIS DATA EXCERPT Sample 1, filtered particles from tractor exhaust gas deposited on nitrocellulose filter The table summari-zes all the most significant analyses performed on the sample.

ANALYSIS OF EXHAUST GAS RESIDUESFILTERED BY A WATER SYSTEM BYNANODIAGNOSTIC ELECTRON-MICROSCOPYINVESTIGATION AND X-RAY MICROANALYSES


TABLE I. ANALYSIS OF EDS 1.


N. ANALISI1234

5

678

910111213

14

15161718

192021222324

DESCRIZIONE PARTICLES ON NITROCELLULOSE FILTER - 47X DUST ON NITROCELLULOSE FILTER - 190X FIBRE DIAMETER 10 µM – 1,122X AGGREGATE 10 µM – 2,350X SPHERULE 1 µM – 2,350X SPHERULE 4 µM – 20,000X BACKGROUND SIGNAL SPHERULE 0.8 µM – 60,000X DEBRIS 0,5-10 µM – 1,291X SHREDDED DEBRIS 60 µM - 800X DETRITUS 35 µM - 800X DETRITUS 130 µM – 1,283X DEBRIS 5 AND 40 µM – 2,613X CARBONACEOUS RESIDUES – 2,000X CARBONACEOUS RESIDUES - 400X SPHERULE 2 µM LEFT – 3,000X SPHERULE 2 µM RIGHT – 3,000X SPHERULES 1-2 µM – 4,297X DETRITUS 2.5 µM – 2,613X DETRITUS 1.5 µM – 2,613X FIBRES - 225X DETRITUS 5 µM - 150X SPHERICAL AGGREGATE WITH 0.2 µM – 41,530X NANOMETRIC DEBRIS DEBRIS 50-250 µM - 300X DETRITUS 50 µM - 300X DEBRIS 5-50 µM - 395X DUST - 90X ACCUMULATE CARBON RESIDUES - 400X DETAIL OF 2 - DETRITUS 2 µM – 2,898X AGGREGATE 5 µM – 16,083X DETRITUS 1.5 µM - 2,123X SPHERULE 0.5 µM – 2,123X

ELEMENTI PRESENTImorphological image morphological image Si,Ca,C,Al,O,Mg,K Fe,C,Cr,Ni,O,Si C,Fe,O,Si,Ca,Ni Fe,O,C,Si C,O,Si,Fe Fe,O,C,Si Fe,C,Cr,Ni,O,Si Fe,C,Cr,Ni,O,Si C,O,Fe,P,Si,Na C,O,Fe,P,Si,Na Fe,C,Cr,Ni,O,Si C,O,Na,Si C,O,Na,Si Fe,C,O,Si,Mn,Na C,O,Ca,P,Si,Zn,S,Fe Fe,O,C,Si C,O,Fe,P,Si,Na Fe,C,Cr,Ni,O,Si Si,Ca,Al,O,C,Mg,Na,Ti Cu,O,Zn,C Ti,O,C,Al,Si,Fe C,O,Fe,P,Si,Na Fe,C,Cr,Ni,O,Si Fe,C,Cr,Ni,O,Si morphological image C,O C,Fe,O C,O,Si,Fe,S,Cl,Ca,Na,Cu C,Fe,O,Cr,Ni,Si C,O,Fe,Si

Analysis 1 and 2 of Table I. The figures show two areas of the sample at low magnification (47x to 190x) where the surface of the nitrocellulose filter on which the particles have been deposited is visible. The Silicon support is visible on the right side of the first image.

EVIDENCE

The residuals analysed by the system applied to the exhaust gas of a tractor highlighted the morpholo-gies and compositions of the particulate matter emitted by the engine. The particles are closely correla-ted with what is contained in the propellant used, lubricants and engine wear residues. Undoubtedly, the tested filter can retain large quantities of particulate matter from the exhaust and play an important role in reducing environmental pollution both from the health and the strictly bureaucratic point of view. Many of the particles captured by the filter are large carbonaceous agglomerates and it should be specified that the volume of sphere-assimilated particles as they legally are is a cubic function of the radius. That means that a small increase in the radius entails a huge increase of the volume and, con-sequently, of the mass. Current law requires that a normal cubic meter of air may not contain more than 40 micrograms of PM10. Excesses are legally sanctioned. It is obvious, therefore, that the removal from the environment of those coarse and relatively heavy particles will result in a remarkable reduction of the particles contained in a normal cubic meter of atmospheric air. Therefore, a widespread use of the system will greatly reduce the number and amount of the sanctions that public administrations face. In conclu-sion, satisfaction is expressed about the role the filter can play in reducing environmental pollution. However, a more in-depth study to assess the influence of fuels and lubricants on the qualitative and quantitative production of particulate matter is recommended in order to evaluate the efficiency over time of the filtration system in question when the water is saturated with pollutants and check what, in terms of particles, may eventually elude the filter and escape in the environment.



This Report shows the results of the research and investigation about the bactericidal and bacteriostatic activity operated by an organic-based deterging agent on two surfaces: floor and desk.The detergent is constituted by medium-short chain fatty acids and their esters.Concentration 0.75% w/wThe method used is a test in plate:PCA count agarIncubation for 48 h at 30 ° CMicrobial count in CFU/m2

Packtin detergent at the concentration of 0.75% shows an inhibition against the microorganisms involved in the study.

pg.01/02

Report on the activity of Research andDevelopment of an organic detergent forthe microbiological control of the surfaces.

Microbial count on the desk surfaces in CFU/m2

Time (h)0624

Comparative example: Not cleaned desk surface3.11x105

3.11x105

3.11x105

Cleaned desk surface with DISINFECTANT C of the invention0

4.5x103

1.35x104

Microbial count on the untreated surface areas and on the cleaned surface areas, in CFU/m2

SurfaceFloor 1Floor 2Desk

Not cleaned before incubation at 30°C:comparative examples (CFU/m2)

9,75x10 4

6x10 3

6x10 3

Cleaned desk surface with DISINFECTANT C of the invention

3000

10


This Report shows the first results of the research, development and the operational test of an orga-nic-based repellent to mosquitoes for the application inside the water tank of the AERIA 400 air purifying machine. It has been developed a repellent agent with activity against mosquitoes based on natural essential oils and volatile acids. Concentration 0.5% (in water) w/wThe repellent was firstly tested against tiger mosquitoes (Aedes albopictus spp.). The method used was:• Type of test: in vivo• Concentration: 0.5% in 10 lt of bi-distilled water vs control (only water)• Environment: a camper positioned near an agricultural waterway (via Caprì 2, 42015 Correggio, RE, Italy)• Duration of the test: 3 h• Two replications• Mosquito bait: human presence into the camper• Count method: Insect count during the test

Packtin repellent at the concentration of 0.50% shows a repulsion against tiger mosquitoes. The future aims are to try its repellent action also against flies and common mosquitoes.

N. Replication

Control 1

Control 2

Test 1

Test 2

N. Mosquitoes

350

380

3

20

Report on the activity of Research andDevelopment of an organic repellent tomosquitoes applied to AERIA 400 airpurification plant.


This Report shows the results of the research, development and the operational test of an organic-based sanitizing agent for the microbiological control of the water tank of the AERIA 400 air purifying machine, as per the Research and Confidential Assignment Agreement signed by both sides on 3/11/2017. It has been developed a sanitizing agent with a biocide and biosta-tic effect based on medium-short chain fatty acids and their esters. Concentration 0.375%, 0.75%, 1.5%, 3% w/w The following report (3 of 3) describes the antimicrobial activity of the sanitizer against Legionella Pneumophila The method used is a Kirby-Bouer Diffusion test in plate: • Inoculum size: 100 microliters of physiological solution with microbial load of 1x107 CFU / ml.• Paper disk dimensions: 5 mm• Incubation for 72 h at 35 ° C

Activity against Legionella pneumophila

Packtin sanitizer at all concentrations shows an interesting inhibition against Legionella pneumophila.


% w/W of sanitizer 0.3750.751.53

Inhibition zone dimension (mm) 16.216.421.925.3


This Report shows the results of the research, development and the operational test of an organic-based deterging agent for the microbiological control of the water tank of the AERIA 400 air purifying machine, as per the Research and Confiden-tial Assignment Agreement signed by both sides on 3/11/2017. It has been developed a deterging agent with a biocide and biostatic effect based on medium-short chain fatty acids and their esters. Concentration 0.75% w/w. The

following report (2 of 3) describes the antimicrobial activity of the sanitizer against four food pathogens bacteria. The method used is a Kirby-Bouer Diffusion test in plate:• Inoculum size: 100 microliters of physiological solution with microbial load of 1x107 CFU / ml.• Paper disk dimensions: 5 mm• Incubation for 24 h at 30 ° C

Packtin sanitizer at the concentration of 0.75% shows an inhibition against the microorganisms involved in the study. The best activity is against Gram + bacteria (Campylobacter and Listeria). The best result is observed against Listeria monocytogenes with an inhibition area of 12 mm.

Report on the activity of Research andDevelopment of an organic detergent forthe microbiological control of water in theAERIA 400 air purification plant.

MicroorganismCampylobacter jejuni

Escherichia coliListeria MonocytogenesSalmonella typhimurium

Inhibition zone dimension (mm) 9,79,2

12,07,9


This Report shows the results of the research, development and the operational test of an organic-based deterging agent for the microbiological control of the water tank of the AERIA 400 air purifying machine, as per the Research and Confidential Assignment Agreement signed by both sides on 3/11/2017.It has been developed a deterging agent with a biocide and biostatic effect based on medium-short chain fatty acids and their esters.Concentration 0.375%, 0.75%, 1.5%, 3% w/w The following report (3 of 3) describes the antimicrobial activity of the detergent against Legionella Pneu-mophila.The method used is a Kirby-Bouer Diffusion test in plate:• Inoculum size: 100 microliters of physiological solution with microbial load of 1x107 CFU / ml.• Paper disk dimensions: 5 mm• Incubation for 72 h at 35 ° C

Activity against Legionella pneumophila

% w/W of sanitizer0.3750.751.53

Inhibition zone dimension (mm)16.216.421.925.3

Packtin detergent at all concentrations shows an interesting inhibition against Legionella pneumophila.

Report on the activity of Research andDevelopment of an organic detergent forthe microbiological control of water in theAERIA 400 air purification plant.

test report n. eg 180406-05 · 2020. 4. 28. · uni en iso 16911-1 . solide a srl - p.iva e cf...

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