OMEGA RESEARCH & DEVELOPMENT AND TRAINING CENTRE

PROJECT WORK

TRAINING&

For students of M.Sc./B.Sc./B.Tech. of

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Microbiology Molecular Biology Biotechnology

Chemistry Pharmaceutical Chemistry Biochemistry

Microbial & Food Technology / Allied Sciences

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H TEST HOUSE134, Ind. Area, Phase-1, Panchkula (Haryana). Phone : 0172-2576904, 5057358(O), Fax : 2571008,

Mobile : 9815000244, 9779224444, 9855445539. E-mail : jugalkg@gmail.com, oth_pkl@rediffmail.com,

omegapanchkula@gmail.com. Website: www.indiamart.com/omegatesthouse

MICROBIOLOGY, MOLECULAR BIOLOGY, BIOTECHNOLOGY, CHEMISTRY, PHARMACEUTICAL CHEMISTRY,

BIOCHEMISTRY, MICROBIAL & FOOD TECHNOLOGY / ALLIED SCIENCES

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HOmega Test House came into being on 1st day of the new millennium as a chemical test house. Over the years its business increased manifolds under the guidance of a team of dedicated professors, chemists, scientists and engineers. The test house has grown into a multi disciplinary test house for fertilizers, micronutrients, food and pesticides, herbicides, weedicides, inorganic and organic chemicals, metals, liquid effluents, water used for different purposes like drinking, irrigation, construction, chemical processing, industrial cooling. It got accreditation by NABL for the above fields for chemical testing in the year 2002. Alongside the test house also caries out mechanical testing related to construction materials for highways, buildings and bridges. Pharmaceutical Testing and training programmes are being planned as new disciplines.

Omega test house provides hands-on, intensive, job focused training, you can bring home and apply immediately on the job. Our mission is to establish unprecedented nationwide education, training and applied research in biotechnology, microbiology, molecular biology, pharmaceutical chemistry and associated technologies. As industry trends change, we evaluate our curriculum and add new concepts to provide the most current information in laboratory and lecture sessions. Massive setup of educational institution in private and public sector is taking place due to globalization of Indian economy. However, some of these institutes are found lacking in giving adequate attention to laboratory work. With requests pouring in from different sectors, we have decided to fill up this gap by providing laboratory exposures to the students to make them more confident to face the challenges for employment in different segments of industry. During the training programs the participants will be interacting with experts in their fields and will get acquainted with practical aspects of various modern techniques along with the underlying principles. Experiments will be conducted in small groups under expert supervision where the individual participants will follow experimental protocol designed in different areas.

Training Facilities at Omega Test HouseOmega test house has facilities for carrying out research and training program in the areas of biotechnology, microbiology, molecular biology chemistry, pharmaceutical sciences. Major equipment and facilities such as high performance liquid chromatography, (HPLC - Waters), Gas chromatography (G.C.), P.C.R., Lab scale Fermenter, Uv-vis-Spectrophotometer, D.N.A. & protein Electrophoresis system, high speed refrigerated centrifuge, Light microscope with projection system, (F.T.I.R.S & A AS - under acquisition)

About Us

Training Section

Molecular methods are used extensively in modern research and development programs of different industries as well as in the field of Biological Sciences. This module is prepared for those who are interested in learning both fundamental and advanced level molecular biology techniques. The course has been designed to give a comprehensive idea of molecular biology; it includes DNA isolation, manipulation of DNA by cloning, amplification of DNA and analyses of DNA by different bioinformatics tools:

Module A: MOLECULAR BIOLOGY

INSTRUMENTATION U V / v i s i b l e

spectrophotometer, laminer air flow, microscope, pH meter, autoclave, centrifuges, shakers, water purification system, auto pipettes, weighing balances etc.

Electrophoresis apparatuses, T r a n s i l l u m i n a t e r , P C R ,

Introduction to recombinant DNA tools and genetic engineering techniquesGeneral rules to perform molecular biology experimentsPreparation of solutions for the molecular biology experiments.Isolation of plasmid, genomic, metagenomic DNA.Agrose gel electrophoresis of isolated DNA.Spectrophotometeric estimation of quantity and purity of isolated DNA.

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Six Weeks - (Fee Rs. 8000/- per Student)

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Restriction digestion of DNA.Ligation of fragmented DNA in the plasmid.Trasformation and screening of recombinantsSelection of the recombinant of interest by master and replica plating.Designing of primersPCRWestern BlottingPlasmid curingAnalyses of the DNA sequences.Retrieving different DNA sequences from the gene banksAlignmnt of DNA sequences using different softwares.Analyses of the derived protein sequence using bioinformatics.

Microbiologocal analysis is done in different industries e.g. pharmaceutical, food, fermentation etc.. Besides this microbiological analysis of different clinical samples is an important tool in the diagnosis of diseases. Theoretical knowledge in microbiology is not sufficient to practise microbiology as microbiological techniques require specialized skills & training. This module is for those who are working or going to start their work in the field and want to expand their scope by earning proficiency in the techniques of practical microbiology.

Module B: MICROBIOLOGICAL TECHNIQUES

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HINSTRUMENTATION UV/ visible spectrophotometer, laminar air flow, microscope, pH meter, auto claves, centrifuges, shakers, water purification system, auto pipettes, weighing balances etc.

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Preparation of microbiological media (Basal medium, enrichment medium, selection medium etc.) Sterilization techniques (Dry heat sterilization, moist heat sterilization, chemical sterilization). Culture techniques (Spread plating, pour plating, streaking, stab cultures).Shake flask fermentation for enzyme production.Staining techniques (simple staining, differential staining, Gram staining, acid fast staining.Wet mount and hanging drop preparation.Identification of bacterial isolates by biochemical methods. Preservation of microorganism .Processing the different clinical samples. Antibiotic sensitivity testing of different clinical samples.Microbiological examination of water.

For analysis of any industrial sample thorough knowledge of different advanced technique

eg.HPLC & GC is essential. Further the knowledge of working of other instruments eg. Fermenter

is required to monitor the production at industrial level in Pharmaceutical, Food and Fermentation

industry. This module covers information immediately applicable to work environment, including

instrument theory, operation, routine maintenance, common problems and solutions.

Module C: ADVANCED TECHNIQUES

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INTRODUCTION & PRINCIPLES of HPLC - G.C. AND THEIR COMPONENTS. The various Modes of chromatographic separation(Reversed phase, Normal phase etc.) Understanding working of HPLC, degassing the mobile phase and preparation of sample.To control retention and selectivity in reversed-phase LC.Concept of gradient elution Analytical method validation and troubleshooting Introduction to gas chromatography(GC). Column types and selection. Sample introduction techniques Reviewing , liquid, and other injection methods. FID detection systems Analysis and interpretation of results. Routine maintenance and troubleshooting

INTRODUCTION TO FERMENTER FUNCTIONINGMedia formulation for enzyme productionSterilization of fermenter, media and ancillary equipments Demonstration of Fermenter functioning Inoculation of media Monitoring the growth &Enzyme production Decontamination of fermenter & ancillary equipment

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Three Weeks - (Fee Rs. 8000/- per Student)

This module has been designed for the persons of Pharmaceutical chemistry and chemistry, who are working or going to start work in industry. This module will give exposure to the candidates on the various tests which are done in the Pharmaceutical and Chemical industry. The emphasis will be given to make the students familiar with the advanced instruments used for the analysis of industrial products.

INSTRUMENTATION HPLC, GC, UV-VIS-spectrophotometer, dissolution app., pH meter, auto pipettes, weighing balance, centrifuge, water purification system etc.Testing of pharma products eg. Tablets, capsules. Syrups, injections etc.Wet chemical analysis of Pharma Products.Qualitative/ Quantitative analysis of chemical samplePracticals in Volumetric/ Gravimetric/ Colorimetric analysisWater analysisIntroduction & principle of HPLC and its various components. The various Modes of chromatographic separation (Reverse phase, Normal phase etc.)Understanding working of HPLC, Degassing the mobile phase and Preparation of sample.To control retention and selectivity in reversed-phase LC.Concept of gradient elutionAnalytical method validation and troubleshootingIntroduction to gas chromatography (GC). Column types and selection. Sample introduction techniquesReviewing of injection methods. FID detection systemAnalysis and interpretation of results. Routine maintenance and troubleshooting

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Four Weeks - (Fee Rs. 8000/- per Student)

Module D: Pharmaceutical Chemistry / Chemistry

Four Weeks - (Fee Rs. 6000/- per Student)

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HModule E: All In One(Microbiology, Molecular biology, Pharmacology, Advanced techniques)This module has been designed for the students who have preliminary knowledge of Microbiology/ Biotechnology/Biochemistry etc. The module has been designed in such a way that student will learn, starting from the basic techniques to the most advanced techniques of life sciences which have direct application in different industries. Along with learning the techniques the students will be taught how to design, perform and analyze the experiments so that they develop interest in the areas of scientific research.

Pharmacology

MicrobiologyMolecular Biology

Virology

Six Weeks - (Fee Rs. 8000/- per Student)

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INSTRUMENTATION UV/ visible spectrophotometer, PCR, Electrophoresis transilluminator, laminar air flow, microscope, pH meter, autoclave, centrifuge, shaker, water purification system, auto pipettes, weighing balance etc.Basic knowledge of designing, performing and analyzing the experimentsCollection of samples from different sources using different techniquesMedia preparation and sterilization techniquesStudying the type of microbial flora of different samplesStaining techniques (simple staining, differential staining, Gram staining, acid fast staining.Screening of organisms for the production of enzyme producing isolates (Proteases, Cellulases, Xylanases,

Amylases, Lipases, Mananases etc).Shake flask fermentation for enzyme production.Identification of isolates by biochemical methods.Monitoring the growth and enzyme production.Enzyme production at lab scale fermenterBasic characterization of the enzyme.Isolation of plasmid DNAIsolation of genomic DNAAgrose gel electrophorese.Testing the pharma products eg Tablets, Capsules, Syrups, injections etc.Introduction and principle of HPLC and its various componentsIntroduction to Gas Chromatography (GC).

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Module F: Food & Fermentation Technology This module has been designed for those who have to work in food or fermentation industry. The candidates will be exposed to various tests done to determine the quality of various food products in the industry. The candidates will also be made well versed with the use of advanced instruments like Fermenter, HPLC, GC, etc.

Four Weeks - (Fee Rs. 8000/- per Student)INSTRUMENTATION UV/ visible spectrophotometer, laminer air flow, microscope, pH meter, autoclave, centrifuge, shaker, water purification system, auto pipettes, weighing balances etc.

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To determine bacteriological quality of raw milkMBR (Methylene Blue Reduction test)Resazurin reduction testSPC (Standard plate count)DMC (direct microbial count)Rapid plate form testsTo determine the bacteriological quality of various food products eg. Ice-cream, butter, condensed milk, etc.

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Quantification of reducing, non reducing, and total sugar in various foods eg. Milk & milk products, fruit juice, etc.Quantification of starch in various food products eg. Cereals.Quantification of fat content in various food products.Introductions to Fermentation and system types, Media formulation for enzyme production.Sterilization of fermenter, media and ancillary equipments.Demonstration of Fermenter functioning & process piping Inoculation of media.Monitoring the growth & Enzyme productionMicrobiological examination of water.

Module G: Project workFour Months (Fee Rs. 25000/- per Student for a group of minimum 5 students)

Facilities exist for the longer term project workin the above areas.

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Molecular Biology

Microbiology

Advanced Techniques for the analysis of industrial products

Pharmaceutical Chemistry

Water Testing

Registration

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Admission for summer training opens in the first week of December on first come first serve basis. Training courses during winter session are also available.

20% deposit is essential for registration in a course. The remaining 80% to be paid at the time of joining the course.

Registration fee can be submitted as under -

Cash DD issued in favour of Omega Test House payable at Panchkula.

Deposit to bank A/C No. 51631131000801. OBC, Sector 8, Panchkula

Registration fee and registration form can be submitted personally or by post to our office at the following address:

Omega Test House, Plot no. 134, Ind. Area, Phase-1, Panchkula, Haryana -134109.

Note: The contents of the modules can be rescheduled depending on the need of a batch of students.

Prof. J.K. Gupta - Prof. Emeritus in Microbiology

(Ex-Dean University Instructions)Panjab University, Chandigarh

Patron:

Faculty:1. Dr N.Kumar - Consultant - Microbiology & Molecular Biology

Under IIPP Scheme of PU, Chandigarh

2. Eminent retired professionals from industry.

3. Retired Professors from PU & other academic Institutions.

For Additional Information

CONTACT:Mr. Manu J Singh - 9815000244 -

me.mjsingh@gmail.comMr. Joginder Singh - 9779224444

oth_pkl@yahoo.inDr N.Kumar - omegapanchkula@gmail.com

Dear Sir/ Madam,

I am submitting herewith my duly completed registration form as detailed below-

Name:………………………………………………………………… Sex:……………………….

DOB…….……………...... Father's Name …………………………………………………………

Mobile no……………………...…… Mobile no (Father/ Guardian)………………………………..

E mail Id …………………………………………………………………….………………........…

Class……………………………… Department……………………………………………............

College/ Institute………………………………………………………………………………........

…………………………………………………………………………………..……………..........

Address of College or Institute………………………………….……………………………...........

…........…………………………………… City…………………........… State……………………

Permanent Address………………………………………………….………………………………

…........…………………………………… City…………………........… State……………………

Address for Correspondence ………………………………………………………………………..

…........…………………………………… City…………………........… State……………………

Please Tick the module of your Interest-

Module A Molecular Biology

Module B Microbiological Techniques

Module C Advanced Techniques

Module D Pharmaceutical chemistry & Chemistry

Module E All in One

Module F Food & Fermentation Technology

Module G Project work

Note: The trainees can also send registration request on a photocopy of the registration form alongwith 20% payment.

Tentative date of joining………………………………………….

Payment details

and the fee amounting Rs

(in figure)………………… (in words)…………………………………..……………………

Registration fee Rs…………………………………DD no………………………………… Date……………………… Name of Issuing bank……………………………………………

on account of Registration fee for the above training.

Date- Signature

Place- (Participant)

Verified and recommended for participation in training.

(HOD/ Principal)

Training Registration Form

TERMS OF PAYMENT

Students are required to pay their fees in 2 instalments as follows:

20% of the fees upon registration (Non-refundable)

80% of the fees at the joining of Training Programme

MODES OF PAYMENT

1. Cash

2. Cheque/DD made out to Omega Test House, Panchkula

3. Deposit to Bank a/c no. 51631131000801 - OBC, Sec tor- 8, Panchkula

Terms & Conditions

These terms and conditions together with the registration form comprise the “Agreement” pursuant to which Omega Test House provides training programs to the students.

1. Trainees must ensure that they bring along when attending a course a personal ID for identification purposes.

2. A 20% deposit is required in order to register in a course. The remaining 80% should be paid at the joining

time of course (Non-refundable). Omega Test House reserves the right to cancel a student's registration and refuse entry to the courses if a student does not settle his/her fees in accordance with the payment terms. 10% concession in fee will be given to first 5 students in every module. Group registration is available for 10 students on call to the contact phone no's.

3. Any fees paid are not refundable and non transferable to other trainees. However Fees paid are

transferable to other courses where a trainee drops a course and registers in a new one. No midway changes allowed.

4. Each training session will be of 4-5 hours a day and 5 days a week.

5. Omega Test House reserves the right to cancel, reschedule, or change the location of a course, if in the

opinion of Omega Test House, such an action is necessary. Omega Test House will notify the students as soon as the change is made. In such a case the student has the option to reschedule the course, apply the fees to another course, or receive a credit note for the course fees paid to Omega Test House.

6. The training certificate shall be distributed only after successful completion of training program.