TEM – Track Etched Membranes Unique technology - Precise Features - Advanced Performance

� Polycarbonate / PC

� Polyethylene Terephthalate / PET

� Polyimide / PI

Track-etched membranes (TEM) are manufactured from either Polycarbonate (PC), Polyethylene terephthalate (PET) or Polyimide

(PI) with such precise and accurate pore size distribution that they are perfect for a varied range of applications from selective

bacterial determination in the food and beverage industry to selective particle removal for further assessment.

These membranes are manufactured using the latest production methods and following strict procedural methods in a clean

room atmosphere (as per ISO 5 and 7): A high purity polymeric film is in the first instance subjected to a tightly controlled high

energy beam of heavy particles which leave the film with a known density of holes. The film is then subjected to chemical

etching with the resultant film having cylindrical ‘holes’ of a uniform diameter. The whole process being controlled by state of

the art technology so the resultant product’s physical properties can be modified to be precisely the pore size and distribution

required by each application.

Key Features:Pore Size: �

The accurate pore size of 0.01 to 30μm ensures that

100% of the larger than nominal pore size liquid or

gas particles are captured on the surface and do not

penetrate the membrane facilitating easy capture and

harvesting.

Pore Density: �

Pore densities available range from 1x104 to 6x109/cm²

with a porosity of up to 30%.

Purity: �

The extremely low level of extractable components

and the inherent chemical inertness of the membrane

materials result in a product with high biological

compatibility: non-cytotoxic and non-bactericidal

behavior, with low binding of proteins (< 5μg/cm²)

and chemicals (like dyes) which guarantee low

background levels after staining cells on the membrane.

The polymeric base material of the TEM does not leach

particles like some other filtration membranes.

Appearance: �

The PC and PET polymers are also both available as

truly black membranes allowing the best possible

contrast and visibility for evaluating microorganisms

stained with fluorescent dyes under epifluorescence

microscopy.

Surface: �

The standard version of all membranes shows a

translucent opacity and two shiny and smooth

surfaces. The optional transparent membrane surface

allows excellent visibility, which is ideal for analyzing

microorganisms and particles under the microscope

using transmitted light.

Physical Properties: �

Polycarbonate, Polyethylene terephthalate and

Polyimide membranes all have inherently high

tensile strengths and good solvent, acid and base

resistance - with PET having a slightly higher

resistance to most organic solvents, amides,

halogenated hydrocarbons and corrosive agents -

making PC, PET and PI membranes a reliable

alternative to PVDF and PTFE membranes.

The maximum operating temperatures of PC and

PET being 140ºC and 150ºC respectively whereas

the PI membrane has an additional advantage of

being operational in temperatures up to 400ºC.

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Accurate pore size

Typical Applications

The smooth membrane with glossy surfaces allows retained particles to be easily viewed under a microscope or electron

microscope. The optional highly transparent membrane, which is recommended in this case, is an ideal medium for a broad

range of analyses as they assure homogenous transillumination with high contrast.

Environmental Analysis, Cell Biology, �

Microbiology, Parasitology, Air Analysis

Our black PC and PET membranes are

made for rapid detection and enumeration

of particles and microorganisms in

epifluorescence microscopy, which requires a

pitch black background. They are treated using

a unique manufacturing process, ensuring a

membrane that although visually grey in colour

appear true black under the microscope with

zero auto-fluorescence that does not stain, for

high visibility and contrast, yielding to superior

results compared to results obtained with other

grey membranes.

Microorganism Detection in Water, Food and Dairy Products, Wine and Beverages, Pharmaceuticals and Cosmetics �

Being manufactured from pure polymeric film gives exceptional chemical cleanliness. They are free of contaminants and have

a very low level of nonspecific protein binding and a low tare weight. These properties give a clear advantage for sampling, cell

enrichment, bacteria and microorganism capture prior to any further analysis.

Microorganism capture, concentration and fractionation �

Yeast �

Mould �

Legionella �

Serratia �

Shistosoma �

Coliform bacteria etc. �

Healthcare �

Biosensors: flow control and protection from potential �contaminants

Diagnostic assays: flow control of reagents, sample preparation and �blood separation

Sterility testing �

Blood filtration and cell analysis: red blood cell (RBC) deforma- �

bility, leukocyte removal, chemotaxis, rare cell detection

Water Analysis �

Absorbable organic halides (AOX) in waste water, drinking water, �soil - even total organic halide (TOX).

Marine biology �

Dissolved phosphates and nitrates �

Air Monitoring �

Airborne particle analysis: pollen, dust, asbestos �

Trace elements �

Analytical Methods �

Gravimetrical analysis �

Qualitative analysis �

HPLC and GC sample preparation �

Cell Culture �

Membranes of PC and PET are available with a special surface treatment for cell culture, �

promoting an exceptional cellular growth and adhesion for a wide range of cell lines.

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Plastic cylinder with a transparent and a black PC membrane as customised design

Tumor cells after filtration

Bacteria collected on PC-membrane

Standard-Products of Polycarbonate (PC), Polyimide (PI) and Polyester (PET)

Membrane diameter 25 mm and 47 mm �

Pore sizes from 0,20 μm to 10 μm �

Pore size [µm]

Thickness PC, PI/PET [µm]

Air flow [ml/min/cm²]

Water flow* [ml/min/cm²]

Bubble pointWater [psi]

Pore density (± 15 %)[Pores/cm²]

0.20 25 / 23 3,5* 10 58 5 × 108

0.40 25 / 23 8.5* 45 32 1,5 × 108

0.60 25 / 23 7.5* 45 17 4 × 107

0.80 24 / 22 16* 90 13 4 × 107

1.0 24 / 22 14* 150 10 2,2 × 107

3.0 22 / 20 18** 350 3 3 × 106

5.0 20 / 18 21** 800 2 4 × 105

8.0 17 / 15 24** 1000 1,3 1 × 105

10.0 15 / 13 30.5** 1150 1 1 × 105

Special Order Products of PC, PET and PI

Pore sizes: 0.01 μm to 0.10 μm and 12 μm to 30 μm �

Rectangular cuts, rolls �

Membrane diameters: 13 mm, 76 mm, 90 mm, 142 mm and 293 mm �

Thinner layer or customised pore density �

Opacity: Transparent �

Hydrophobic or specially treated for cell culture �

Please note: If you are intended to use the PC membrane for AOX, you will require a special controlled quality with 10 μm

thickness. PC, PET and PI membranes can be autoclaved or sterilized by using Ethylene Oxide (EtO) or Gamma irradiation.

Broad Range of Available Characteristics

Feature Polycarbonate (PC) Polyester (PET) Polyimide (PI)

Thickness (μm) 6 – 50 9 – 50 7 – 25

Burst strength (psi) > 10 > 10 >10

Weight (mg/cm²) 0.7 – 6.0 1.2 – 7.0 1.3 – 3.5

Specific gravity (g/cm³) 1.21 1.39 1.43

Heat sealing range (°C) 230 – 275 250 – 265 -

Max. service temp. (°C) 140 150 400

Ash weight (μg/cm²) 0.6 2.3 -

Porosity (%) according to pore size/density 0.1 – 20 0.1 – 20 0.1 – 20

Pore density (pores/cm²) depending on pore size 104 – 6 x 109 104 – 6 x 109 104 – 6 x 109

Pore size (μm) 0.01 -30 0.02 – 30 0.01 –10

Opacity Translucent; optional: Translucent; optional: Translucent

transparent, black transparent, black

Refractive index 1.58 – 1.63 1.55 – 1.66 1.70

Surface treatment Hydrophilic; optional: hydro- Hydrophilic; optional: hydro- Hydrophilic; optional: hydro-

phobic or cell culture treated phobic or cell culture treated phobic or cell culture treated

Water absorption (% wt) < 0.35 < 0.5 <1.0

Fibre release No No No

Leachables Negligible Negligible Negligible

Autoclavable (30 min, 120 °C) Yes Yes Yes

Biological compatibility Inert Inert Inert

Polymer structure Amorphous Stretched (crystalline) Amorphous

*at 10 psi, **at 2.5 psi

Translucent or deep black �

Both sides with shiny surface, hydrophilic �

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Germany

Hahnemühle FineArt GmbH

Hahnestrasse 5

D-37586 Dassel

Sales area

Germany, Austria, Suisse

and the Netherlands:

Phone: + 49 5561 791 687

Sales area

Rest-Europe and other Countries:

Phone: + 49 5561 791 688

Fax: + 49 55 61 79 13 77

filtration@hahnemuehle.com

www.hahnemuehle.com

United Kingdom

Hahnemühle FineArt UK

Suite 6, St. Mary‘s Court

Carleton Forehoe

GB-Norwich,

NR9 4AL

Phone: + 44 (0) 845 3300 129

Fax: + 44 (0) 1603 757 915

ukfiltration@hahnemuehle.com

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Ordering information for PC Membranes

Part number Pore size Diameter Quantity per box

PC02025NL 0.2 μm, black 25 mm 100

PC02025TL 0.2 μm, translucent 25 mm 100

PC02047NL 0.2 μm, black 47 mm 100

PC02047TL 0.2 μm, translucent 47 mm 100

PC04025TL 0.4 μm, translucent 25 mm 100

PC04025TLX 0.4 μm, translucent, for AOX 25 mm 100

PC04025NL 0.4 μm, black 25 mm 100

PC04047TL 0.4 μm, translucent 47 mm 100

PC04047NL 0.4 μm, black 47 mm 100

PC04047TLX 0.4 μm, translucent, for AOX 47 mm 100

PC30047TL 3.0 μm, translucent 47 mm 100

PC50047TL 5.0 μm, translucent 47 mm 100

PC80047TL 8.0 μm, translucent 47 mm 100

PC100047TL 10.0 μm, translucent 47 mm 100

Other membrane types like PET and PI, other pore sizes, diameters, design and pack sizes are available on request.

Please contact our sales office for information on order size and lead time.

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