teachable tidbit: mobility of membrane lipids and proteins i.pre-assessment group discussion...

Download Teachable Tidbit: Mobility of membrane lipids and proteins I.Pre-assessment group discussion II.Lecture/discussion/ Experimental examples III.Post unit

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  • Slide 1
  • Teachable Tidbit: Mobility of membrane lipids and proteins I.Pre-assessment group discussion II.Lecture/discussion/ Experimental examples III.Post unit assessment: problem set and quiz
  • Slide 2
  • The fluid mosaic model for biological membranes: A lipid bilayer within which integral membrane proteins are inserted into and through the bilayer, but unless restrained, free to move within the plane of the membrane.
  • Slide 3
  • Topic/unit objective: Understanding the nature of and functional significance of the dynamics of lipids and proteins in biological membranes Pre-unit assessment goal: How would you demonstrate the mobility of lipids and/or proteins in biological membranes? CRAZY IDEAS WELCOME: Fluid Mosaic Model for Biological Membranes
  • Slide 4
  • I.Modes of lipid mobility; factors that affect mobility II.Topographic organization of biological membrane proteins III.Lateral mobility of integral membrane proteins I.Frye and Edidin experiment demonstrating lateral mobility of cell surface proteins in mouse/human heterokaryons II.Antibody capping of cell surface membrane proteins III.Kinetic analysis of lateral membrane protein mobility: FRAP and FLIP analysis IV.Modes of regulating membrane protein mobility
  • Slide 5
  • Fluorescence Recovery after Photobleaching (FRAP)
  • Slide 6
  • Slide 7
  • Fluorescence recovery after photobleaching
  • Slide 8
  • Nuclear lamina on inner surface of frog egg nucleus. Lamin dimers
  • Slide 9
  • Slide 10

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