A

Figure 1. Periaortic iron in C57 Bl (A) and ApoE K/O (B) mice 6 days following intravenous administration of SPIO (1 mmol/kg iron). Iron uptake is much greater in the periaortic fat of ApoE K/O mice.

B

A

Figure 2. Periaortic Mac3 stain in C57 Bl (A) and ApoE K/O (B) mice. There is greater positivity in the latter. Mac3 x10.

B

A B

Figure 3. Subcutaneous tissue iron in C57 Bl (A) and ApoE K/O (B) 6 days following the intravenous administration of SPIO (1 mmol/kg. iron). There is no significant difference in iron uptake.

A

Figure 4. Subendothelial and periaortic fat iron in WHHL rabbit (A) 6 days following intravenous administration of SPIO. In this case, fat uptake was greater than subendothelial. RAM-11 staining (B) shows positivity in the plaque and periaortic fat.

B

A

Figure 5. A. Human atherosclerotic coronary artery with prominent necrotic core and macrophage infiltration. x4 B. CD68 demonstrates heavy macrophage concentration in the plaque but also in the pericoronary fat. C. Inset shows in higher magnification the vascular-stroma positive population.

C

B