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Task 3:Sampling for LarvaeAlso called wrigglers!

Imagine small, the size of a pencil tip, creatures that wriggle from side to side.

Visit: http://youtu.be/QRpv7aNtQiY

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Task 3. Sampling for mosquito larvae

Counting Mosquito Wrigglers Protocol

Goal: To sample mosquito wrigglers in breeding habitats around Baltimore, Maryland.

Read over materials and data collection sheets before starting your own sampling.

1. Sample during the first week of May 2014. 2. On top of Wriggler Data Sheet, write your

street address in the ID section (all information given in this project is strictly confidential). Do this for all sheets every sampling time. Write the date on every sheet for each sampling time.

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Task 3. Sampling for mosquito larvae

3. To sample:-Begin by walking around the property of your residence and note any

potential habitats. Be sure to review the Mosquito Habitat Identification worksheet, which outlines examples of mosquito habitats and how to measure them. -Designate 5 to 10 potential habitats around the property you think would have wrigglers. Make sure to remember where/what these habitats are. You are only sampling wrigglers from wet habitats with standing water.

-Each mosquito habitat will be successively numbered under Plot ID starting from 1.

-For each mosquito habitat with standing water, you will take a 1mL sample of water for a visual inspection for wrigglers. To take the sample, squeeze the bulb of the pipette and insert the tip into the standing water. With the tip under water, release the bulb and the pipette should fill with water. Empty the pipette onto a petri dish and visually inspect the sample. Count the number of wrigglers in sample and record on Wriggler Data Sheet. Empty petri dish and rinse with water before moving onto next sampling area.

-Continue along your route, repeating these directions until you have returned to the starting location.

4. For each sampling period, you should use the same sampling technique and follow the same route. There may be changes in the number, state, or location of mosquito habitats between sampling periods and this is normal.

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Activity 3. Urban Site and Mosquito Field Study

Mosquito Lifecycle

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Task 3. Sampling for mosquito larvaeWriggler

Data Sheet       Address=  Habitat Codes:   Date=  1= Puddles   4=Buildings/Foundations 7=Tires  2= Swampy Areas   5=Water Storage Container 8=Planters  3=Drain/Ditch   6=Trash   9=Other        

Number your habitat Habitat Code Habitat Description Visual Estimate Are there wrigglers present? Number from sample/////////////// /////////////// //////////////////////////////////// (Yes/No) Yes No 1mL sample

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