TALINUM PANICULATUM (GINSENG JAWA)

20 DAYS

GROUP MEMBERS… NATASHA KAUR AMARPREET KAUR YAASHINI

SELVARAJAH KRISNAVIMALA

KRISHUAN NABILAH BINTI

YUSOF NORIZA BINTI

ISHAK

AMIRAH BINTI SHAARI

HILDA ANAK EMAK AIN NAJWA BINTI

AMRAN NURUL HIDAYAH

BINTI MANSOR ONG SHWU CHYN PUI WAN TING WONG SIEW CHING

INTRODUCTION

Five Mus musculus have been

used to investigate the effect of Talinum Paniculatum (ginseng jawa) to the liver, kidney and intestine within `20 days of experimental.

Problem statement

Why do we choose Mus musculus as our specimen?

• Class of mammalian• Easy to get• Easy to handle-small in size

Purpose of the study

To know the effect of Talinum Paniculatum size and body weight of Mus musculus

Behaviour investigation of the Mus musculus.

Significance of study Talinum Paniculatum contains

vitamin c and toxic

Tanin, Saponin and Flavoid.

So we carry out this experiment to see the side effects of Tanilum Paniculatum to the internal organs (intestine, kidney and liver) of Mus musculus.

Research limitation

Experiment applicable only to five Mus musculus.

only three leaves of Tanilum Paniculatum used everyday.

Time limitation to only 20 days

LITERATURE REVIEW

1.Talinum:

A Summer Vegetable For Florida

DAVID FAIRCHILD, COCONUT GROOVE, FLORIDA

DR J.L HEID said that in his report :In the green leaves of Talinum

The Vitamin C is about 160 mg per 100 gram

The Talinum show the phenomenally high content of vitamin C compared to guavas and sweet peppers.

It also probably has a considerable contents of carotenoid pigments.

2The Effect Of Ginseng Jawa Root (Talinum Paniculatum) To The White Male Mencit

Evi Setiya ManuputyFaculty Of Pharmacy

University Of Muhammadiyah Surakarta

Chemical content in Talinum paniculatum composed of saponin, flavanoid and tanin.

Saponin - strengthen our body and - improve blood

circulation. Saponin : can cause irritation in

mucosa membrane, and can damage red blood cell through hemolysis

The root can be used to treat : tiredness, dizzy, diarrhea, aphrodisiacs and pneumonia.

The leaves can be used to treat bump. Also increase the sexual desire in male

mencit.

Materials and methods

20 days of observation5 mice (Mus musculus)Bedding3 ginseng jawa (Talinum paniculatum)

leaves (0.5g) mix with 100 ml waterMice food

Mice is weighed daily Amount of water consumed are

measured

DissectionGloveCotton and chloroformDissecting setDissecting tray and pinsWaterBeaker

Water was applied on the ventral side of mice

The kidney, small intestine and liver were dissected.

Stages/technique in preparing slide

Fixation. Dehydration. Clearing. Embedding. Sectioning. Coloring/ staining.

FixationBouin’s fluid (<24 hours)Bottles

DehydrationEthanol 70% (< 24 hours)Ethanol 80%, 95%, 100% (< 1 hour)

ClearingToluene solution (< 24 hours)

Embedding (Paraffin processing)

Paraffin solution (60C) (< 1 hour) Paraffin solution (60C) (< 1 hour)

• (twice procedure)

Paraffin solution added in mold followed by the organ.

Tissue sample then was left for 5 days.

The infiltrating medium (paraffin) provides cellular support for cutting tissue sections.

Sectioning (Microtome /cutting) materials methods

Diamond penDistilled waterAlbuminSlide and slide

coverMicrotomeBrush

The slices was placed on the slide.

Few drops of distilled water/albumin are dropped on the slides. Intestine-distilled

waterAlbumin - liver and

kidney Heating (30C)

Coloring/staining

Materials Methods Xylen 1 And 2 Alcohol+xylen (1:1) Alcohol,

Alcohol( 90%,80%,75%) Distilled Water Hematoksilin Solution 0.5 % Sodium Bicarbonate

Solution Running Water Scott’s Solution Eosin

Color stain used :• Harris Hematoksilin- Eosin

coloring.

• Soaking of slides- • into 21 piece of jar

containing different types of chemicals and liquid

• based on the duration given.

• Gluing: DPX / Canada balsam

• Heating of slides • (37 C) • 1 week

Results & discussion

day 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17Resu

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Graph showing the increasing weight mass of the mice in 20 days

The weight of each mice increase day by day After taking Talinum paniculatum as their drink.

The Talinum paniculatum rich with vitamin Cincreases appetiteGaining of body weight

Graph showing the amount of water and Talinum paniculatum consumed by the mice's

Intestine (duodenum)

Affected intestine

The goblet cell in this intestine secretes more mucous compared to the normal intestine

40x magnification

Goblet cell

LIVER

Affected liver Normal slide

Wide spaces in between cells showing the sinusoid. This means that more enzymes are secreted to detoxify the drug (ginseng jawa). In the normal slide the sinusoid is compact.

sinusoid

40 x magnification

The presence of vitamin C in Talinum paniculatum causes more enzyme to be released to detoxify the drugs in the liver cells.

prove: white spaces (sinusoid)seen in effected liver cells while the original cell the (sinusoid) is more compact.

KIDNEY

Affected kidney Normal kidney

The glomerular is tightly packed due to the toxic caused by the drug that is being detoxify by the glomerular as compared to the normal kidney slide.

glomerulus

40x magnification

Materials & function:• act as rinsing agent to remove the blood from

the organs, and its sterileNormal saline solution

• a fixing & preserving solution for the organsBouin’s solution-

• For dehydration processEthanol 70%,80%,95% & 100%

• used for the clearance purposeToluene

• to hold the organ tissues tightly during the sectioning process

paraffin

• Only the small intestine cell can withstand with the pressure.Distilled water

• to maintain the shape of the kidney and liver cell.Albumin

Effect of Talinum

paniculatum

Increase the appetite

Improve blood circulation

Stimulate testosterone hormone to

enhance sexual desire

they will get attracted to mate becomes active and aggressive

Weight gain

Tannins precipitate proteins inhibits in some ruminant animals for the absorption of nutrients from high-tannin

grains such as sorghum.

Tannic acid does not affect absorption

of other trace minerals such as zinc, copper, and manganese in rats

Prove: the glomerulus at kidney cell is tightly packed compared to the original slide of kidney where there are some spaces between the glomerulus.

The lethal dose of Talinum paniculatum around 6% is able to increase the animal's body weight.

Prove: the mice’s body weight increases drastically in that 20 days.

Problem about the project

Damage of the weighing machine.

Damage of Microscope.

The end..Thank you..