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iGEM – The International Genetically Engineered Machine Competition and The Registry of Standard Biological Parts Presidential Commission for the Study of Bioethical Issues Randy Rettberg, Sept. 14, 2010

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Page 1: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

iGEM – The International Genetically Engineered Machine Competition

and The Registry of Standard Biological Parts

Presidential Commission for the Study of Bioethical IssuesRandy Rettberg, Sept. 14, 2010

Presenter
Presentation Notes
* Introduce how iGEM works to everyone Summarize what happened and what will happen with iGEM
Page 2: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

The TTL Data Book

Page 3: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

The Registry of Standard Biological Parts

Page 4: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

Community Parts Collection

Page 5: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

Synthetic Biology Question

Can simple biological systems be built from standard, interchangeable parts and operated in living cells?

Or, is biology so complex that each case is unique?

Page 6: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

Education Driving Research

Page 7: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

Device-Level System Diagram 2003

Page 8: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

Parts- and Device-Level System Diagram

Page 9: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

Year

IAP

2004

2005

2006

2007

2008

2009

2010

2011

2012

Teams

4

5

13

32

54

84

112

130

170

225

Participants

20

70

150

400

750

1180

1650

1900

2500

3300

iGEM Growth and Scale

Projected

Page 10: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

iGEM 2009

Page 11: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

iGEM Sites

Page 12: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

iGEM Sites

Page 13: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

iGEM Philosophy

Get and Give

Teams are expected to use the parts, ideas, and experience of teams in previous years.

Teams are expected to contribute their parts, ideas, and experiences.

Page 14: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

Community Beyond iGEM

Page 15: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

iGEM Statistics

• 6,513 users• 2,478 users have logged in this year• 1,005 have entered parts• 85 labs registered

• 12,327 parts in the Registry• 5,166 samples in the Repository• 2,328 parts reported to work• 1,691 parts sent by iGEM 2009 teams• 800 confirmed parts (of 1000)

Page 16: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

iGEM Schedule: Assemble Your Team

5 High School Students5 Undergraduate Students3 Graduate Students3 Faculty

Utah State - iGEM 2009

Page 17: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

iGEM Schedule: Raise Money

Page 18: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

iGEM Schedule: Attend A Workshop

Page 19: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

iGEM Schedule: Get the BioBrick Parts

Page 20: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

iGEM Schedule: Work At Your School

Presenter
Presentation Notes
Each student teams works at own school
Page 21: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

iGEM Schedule: Attend the Jamboree

Page 22: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

iGEM Schedule: Attend the Jamboree

Page 23: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

iGEM Schedule: Win Awards

Presenter
Presentation Notes
Typically 6-12, had up to 25 Biologists, others… Mostly undergrads (had HS), but GS’s should only be supervisors Instructors are important as well – a way to get synbio into a school
Page 24: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

iGEM Schedule: Publish Your Work

Presenter
Presentation Notes
iGEM 2006 from Edingburgh
Page 25: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

MIT iGEM 2006

eau d’e coli

mit igem 2006

Presenter
Presentation Notes
Hasn’t been explored much in synthetic bio Put Cool, Regulated biosynthetic devices in e. coli for the registry
Page 26: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

Final System

Presenter
Presentation Notes
Change title of cell to indole-deficient Get rid of title of indole knockout chassis in other slides.
Page 27: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

Banana Generating Device

Page 28: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

Precursor Biosynthesis

Presenter
Presentation Notes
Mention: we put these devices together w/ WGD and BGD, and found that they expressed the scent without exogenously adding precursor
Page 29: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

Creating a Red Blood Cell Substitute

Researchers Arthur Yu • Austin Day • David Tulga • Hannah Cole • Kristin Doan • Kristin Fuller • Nhu Nguyen • Samantha Liang • Vaibhavi Umesh • Vincent Parker

Teaching Assistants Amin Hajimorad • Farnaz Nowroozi • Rickey Bonds

AdvisorsJohn Dueber • Christopher Anderson • Adam Arkin • Jay Keasling

Medical Applications

UC Berkeley iGEM 2007

Page 30: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

Creating a Red Blood Cell Substitute

Medical Applications - Bactoblood

Page 31: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

Save the World - Arsenic Detector

A test tube could contain all the necessarycomponents: Freeze dried bacteria, growth medium,indicator powder, Ampicillin salt, etc…

•These tubes could then be given to local villagers to monitor their own water quality themselves

•A good alternative to the widely used Gutzeit method

www.Macteria.co.uk

Presenter
Presentation Notes
Here is the result we got from the modeling. When there is low concentration of arsenic input, like 5 ppb, the lacZ is produced at a basal level while the Urease is quite high.
Page 32: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

Live Forever - BioBeer - Resveratrol

Page 33: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

2009 Winner - Cambridge

Page 34: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

2008 Winner - Slovenia

Helicobacter pylori

Peptic Ulcers

Testing in Mice

Page 35: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

Is iGEM Safe?

Page 36: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

Human Practices - Heidelberg 2008

Page 37: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

Is iGEM Secure?

Page 38: Synthetic Biology & iGEM International Genetically ......iGEM Schedule: Attend the Jamboree iGEM Schedule: Win Awards Typically 6-12, had up to 25 Biologists, others… Mostly undergrads

iGEM Awards: Finalists

2009 2008 2007 2006

Cambridge Slovenia Peking Slovenia

Freiburg Caltech UC Berkeley Imperial

Groningen NYMU Taipei Slovenia Princeton

Heidelberg Freiburg Paris

Imperial Harvard UC San Francisco

Valencia UC Berkeley USTC China