syntech resea · effect studies • oecd 75 semi‐field ... trial types: bioefficacy in...

© July 2018

Expert services delivering competitive advantage

SynTech Research’s range of services include:• Environmental Chemistry (GLP) - Lab & Field• Primary Screens (GEP) - Greenhouse• E�cacy Field Trials (GEP) - Field• Bee Ecotoxicology (GLP) - Lab, Field• Seed/Traits GM & Conventional - Greenhouse, Field• Protocol & Program Design• Study Directorship, Project/Program management

• Quality Assurance

Global provider of�eld and laboratory

R&D services

SynTech Research U.S. Operations

For further information visit www.syntechresearch.com or email [email protected]

Field sitesHQ and Lab Services

- US Headquarters and Lab ServicesStilwell, KS

- Sanger, CA Research Center

- Groveland, FL

- Atlantic, IA

- Stewardson, IL

- Drexel, MO

- Whiteface, TX

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5-Stilwell, KS6Drexel, MO

Sanger, CA

Groveland, FL

Atlantic, IA

Stewardson, IL

Whiteface, TX

Analytical Laboratory SynTech Research Laboratory Services, LLC 17745 Metcalf Ave., Stilwell, KS 66085

Lead By: Frank White – 913‐378‐0998 ext. 106 [email protected]

Analytical Chemistry Manager – PhD in organic chemistry, over 30 years of chemistry experience in Crop Protection Chemicals and Pharmaceuticals.

SynTech Research has assembled an experienced team of residue scientists striving to complete your project on‐time with high quality GLP standards. Utilizing our 7 state‐of‐the‐art triple quad LC mass spectrometers (AB Sciex and Thermo Fisher) and a triple quad GCMS. SynTech Research offers the combination of instrumentation, experience, knowledge, communication and flexibility to provide project deliverables to meet a broad range of analytical needs. SynTech Research conducts its work under U.S. EPA/OECD GLP guidelines. Sample homogenization can be provided for all types of crop and soil samples. A working kitchen is available for residue reduction studies in prepared foods. SynTech Research has nearly 7000 sq. ft. of temperature monitored freezer space. All necessary USDA/APHIS‐issued import permits are available or can be obtained for any type of crop/matrix importation to the USA, including restricted import material permits for rice and sugarcane. SynTech Research also holds a USDA PPQ 525 soil permit for restricted soils. Analytical Team Members: Sean Moore– 20+ years in residue chemistry

Walter (Van) Vandaveer, PhD – 12.5 yrs. analytical chemistry Katie Stone ‐ 10 yrs. in Sample Management Jennifer Shepherd, BS – Analytical Report Writer; 16 yrs. experience Farah Rahimi, MS – Quality Control Data Reviewer

Specialized Areas of Research:

Method development and validation. Method refinement for crops, processed commodities, ecotoxicology studies, animal tissues, animal feeds and pollinator matrices. Independent laboratory validation (ILV). Storage stability. Soil dissipation. Field volatility and spray drift measurement. Operator Exposure studies (Transferable Turf Residue & Dislodgeable Foliar Residues). Non‐target plant (NTP).

Types of services performed: Trace residue analysis

Study Management – Stilwell, Kansas SynTech Research 17745 S. Metcalf Ave. Stilwell, KS. 66085

Lead By: Denise Woodard – Office: 913‐378‐0998 ext. 103 [email protected]

Study Directors:

Denise Woodard Cheryl Lenz Jaime Brungardt Heidi Dixon‐White Chris Banman

Knowledge: SynTech Research Study Directors each have at least 10 years of experience as a Study Director, and have a combined 55+ years of Study Director experience and 100+ years in the agriculture industry. All Study Directors have residue analytical background experience and technical writing abilities for the studies we manage. Work on a wide variety of crops including fruits, vegetables and grains.

Study Types: Manage various residue and environmental field studies such as Processed Food/Feed, Magnitudeof Residue, Import Tolerance, Field Accumulation in Rotational Crops, Animal Feeding, Pollinator, Dislodgeable Foliar Residue, Storage Stability, Soil Dissipation and Post‐Harvest.

What We Do:

Study Management Team offers valuable input and recommendations to the Sponsor relating to field phase design / agricultural management practices. Prepare detailed study protocols following appropriate guidelines.

Coordinate with field cooperators during the study conduct and monitor sample processing and analyses. Ensure that all work is being conducted under strict adherence to GLP Standards.

Provide the necessary technical direction to the various field cooperators and laboratory personnel in order to assure quality work and timely completion of the study.

Prepare final study reports at the conclusion of studies for Sponsors suitable for submission to the appropriate regulatory agency. Incorporate field work as well as analytical and processing data into final study reports. Interpret data based on various environmental factors/field conditions/maintenance practices/analytical challenges.

Guidance:

EPA Residue Chemistry Test Guidelines, OCSPP Series 860 EPA GLPs 40 CFR Part 160 OECD Guidelines for the Test of Chemicals, 500 Series SANCO 7525/VI/95 – rev 10.3 NAFTA Guidance DIR98‐02 and DIR2010‐05 USEPA/PMRA Joint Guidance Doc. SPN2017‐02

Trial Locations: U.S., Canada, Mexico, Central America, South America, SE Asia, Asia Pacific

Terrestrial Laboratory & Field SynTech Research Laboratory Services, LLC 17745 Metcalf Ave., Stilwell, KS 66085

Site Lead: Houston Howerton – Cell: 785‐331‐6971 [email protected]

Houston has a depth of experience across a range of ecotoxicology areas, ranging from aquatic to terrestrial. He has been instrumental in building our terrestrial ecotox lab team in Stilwell, KS. He has extensive beekeeping experience and a high degree of expertise in pollinator effects, residue, and

efficacy studies.

Laboratory Test Capability Overview and Trial Types:

Acute Effects • Honey bee adult oral and contact acute tests (OECD 213, 214 and OCSPP

850.3020) • Honey bee 7‐day larval acute test (OECD 237) • Honey bee acute toxicity of residues on foliage (OCSPP 850.3030) • Bumble bee oral and contact acute tests (OECD 213, 214 and EFSA, 2014)

Chronic Effects • Honey bee 10‐day adult chronic test (Decourtye, 2005 and EFSA, 2014) • Honey bee 8‐day larval chronic test (draft OECD, 2014 modified) • Honey bee 22‐day larval chronic test (draft OECD, 2014 and Univ. Florida

method Apiaries and Crops to Support Pollinators:

SynTech maintains a permanent apiary at the Stilwell location to support field and laboratory studies. Additional supporting apiaries are located in KS and MO, including 15 acres partnered with Kansas State University Horticulture Extension Department.

Field Testing Capability Overview:

Residue (Bee Matrix) Studies • Tunneled Studies with honey bee and bumble bee used as collectors

• Sampling from the hive and from the foragers (pollen load, stomach preparation and nectar extraction)

• Open field studies with manual collection • Pollen and nectar manual collection experience with more than 30

crops Effect Studies

• OECD 75 semi‐field • Full field effects • Feeding studies (Oomen) • Efficacy studies (miticides) • Customized designs

Residues in Bees and Bee Products:

Tunneled Studies: • Whole bees, Pollen, Nectar, Larvae, Wax, Crop Matrices

Pollen Nectar

Residue Crop Experience:

cotton, tomato, cucurbits, citrus, peppers, stonefruit, apple, buckwheat, Canola, sunflower, cranberry, blueberry, clover, soybean, ornamental flowers

Sanger Research Center – EPA Crop Region 10 SynTech Research, Inc. 17915 E. Annadale Ave.

Sanger, CA 93657 Site Lead : Mariana Krugner – Office: 559‐875‐7080

[email protected]

Mariana Krugner ‐ Sanger Research Center Manager. Joined SynTech Research in 2011. Before SynTech, Mariana had over eleven years of research experience on major crop pests and diseases at the University of California and USDA, Agricultural Research Service.

Specialized Areas of Research:

Conv. & GM Seeds/Traits, Yield, GLP Residue Studies, Bioefficacy, Terrestrial Ecotoxicology, Plant Stress Response Screening

Trial Types: Bioefficacy in Laboratory, Greenhouse & Field ‐ insecticides, fungicides, herbicides, plant growth regulators, fertilizers, biostimulants, harvest aids, GMO crops, and seed treatments from early stage screening to post‐sale products Seeds/Traits ‐ nutrient composition analysis, expressed trait protein analysis, agronomic characterization Seed production for conventional and GMO row and vegetable crops Yields ‐ row and vegetable crops GLP ‐ processed food/feed, magnitude of residue, field accumulation in rotational crops, terrestial/pollinator, Dislodgeable Foliar Residue (DFR), Turf Transferable Residue (TTR), non‐target organisms in the lab and field

Main crops at this location:

Orchards and Vineyards including walnut, almond, grape, citrus, apple, and stone fruits Row and vegetable crops including corn, sorghum, soybean, barley, canola, wheat, cotton, alfalfa, tomatoes, peppers, eggplant, okra, cucumber, carrots, cabbage, lettuce, mustard greens, melons, potatoes, squashes, strawberries, sugar beets, sunflower, onions, spinach, brussel sprouts, cauliflower, broccoli, radish, grasses, and ornamentals

Diseases/Pests/Weeds:

Diseases: Fireblight , gray mold, powdery mildew, shot hole disease. Phytophthora spp., Fusarium spp., Alternaria spp., Botrytis spp. in the lab Pests: Egyptian alfalfa weevil, Lygus bug , aphid spp., leafhopper spp., California red scale, citricola scale, San Jose scale, whitefly spp., codling moth, Spodoptera spp., corn earworm, cabbage looper, diamondback moth, soybean looper, thrips spp., twospotted spider mites Common weeds in the CA cropping systems – resistant weeds: (Monocot including sedges: resistant to G1, G2, G9, G22, G10) & (Dicot: resistant to G2, G9, G22)

Soil Type: Fine Handford sandy loam, 1% organic matter

55.763

68.474.9

83.691.6 96.5 96.5

90.879.9

66.456.5

37.9 41.8 45.2 48.655.2 61.1

66.2 65.4 60.952.7

43.438

2.27 1.99 2.48 1.02 0.56 0.22 0.04 0.03 0.33 0.66 1.2 2

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Annual Weather Averages for Sanger, CA

Average High Temp (°F) Average Low Temp (°F) Average Rainfall (in)

www.ncdc.noaa.gov – Source for perception and weather averages

Florida Field Station – EPA Crop Region 3 SynTech Research, Inc.

14750 Lee Rd, Groveland, FL 34736

Site Lead: Tyler Parks – Cell: 217‐972‐8548 [email protected]

Tyler Parks – worked at the SynTech Stewardson IL for 3 years before moving to Florida site. Extensive experience in row crops in the Midwest and most vegetables grown in Florida.

Specialized Areas of Research: Conv. & GM Seeds/Traits, Yield, GLP Crop Residue Studies, Bioefficacy, Pollinator/Terrestrial Ecotox

Trial Types:

Bioefficacy ‐ Insecticide, herbicide, fungicide, seed treatments, biologicals, plant stress GLP ‐ Processed Food/Feed, Magnitude of Residue, Field Accumulation in Rotational Crops, Terrestial/Pollinator Biostimulants and Fertilizers Plant growth regulator evaluations Seeds/Traits: Nutrient Composition Analysis, Expressed Trait Protein Analysis, Agronomic Characterization


Tomato, Pepper, Lettuce, Watermelon, Winter Squash, Summer Squash, Cotton, Bermuda Grass, Cabbage, Eggplant, Okra, Mustard Greens, Radish, Broccoli, Cauliflower. Citrus: Orange, Mandarins, Grapefruit and Lemon, Banana

Diseases/Pests:

Downy mildew, powdery mildew, white fly, bacterial spot, sunburn, hornworm Asian citrus psyllid

Soil Type:

Sand, <1%

71.2 73.978

82.588.1 90.7 91.8 91.6 89.5

84.678.4

72.8

49.2 52.155.8

6066.4

72 73.6 74.1 72.766.4

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2.35 2.38 3.77 2.68 3.457.58 7.27 7.13 6.06 3.31 2.17 2.58

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Iowa‐A Field Station – EPA Crop Region 5 SynTech Research, Inc.

2633 300th Street Atlantic, IA 50022

Site Lead: Shane Andersen – Cell: 712‐404‐0074 [email protected]

Shane Andersen – over 10 years experience as a PFI, 15 years total experience. Specializing in agronomy, weed science, biotech, USDA & EFSA regulations, hand pollination and GLP crop residue studies

Site Information: Family owned farms, isolation and security for biotech work Specialized Areas of Research:

Biotech Seeds/Traits, GLP Residue Studies, Bioefficacy/Yield

Trial Types:

Biotech Seeds/Traits ‐ Nutrient Composition Analysis, Expressed Trait Protein Analysis, Agronomic Characterization, Hybrid Nursery, Seed Bulk‐Up, Processing, Regulated Crop Residue. GLP ‐ Processed Food/Feed, Magnitude of Residue, Field Accumulation in Rotational Crops. Bioefficacy ‐ Herbicide, Foliar Fungicide, Seed Treatments, Biologicals, Yield Trials.


Field corn, soybean, alfalfa, small grains, other row crops


Diseases: Grey leaf spot of corn Pests: bean leaf beetle of soybean Weeds: A variety of species common to upper Midwest

Soil Type:

Silty Clay Loam, Clay Loam, 3‐4% OM

31.536.5

49.6

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82.585.9 83.9

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Annual Weather Averages for Atlantic, IA


www.ncdc.noaa.gov – Source for precipitation and weather averages

Illinois Field Station – EPA Crop Region 5 SynTech Research, Inc. 2911 E. 300 North Rd Stewardson, IL 62463

Site Lead: Gary Schultz – Cell: 217‐690‐1826 [email protected]

Gary Schultz – Over 30‐years experience in crop research and production.

Sam Eident – Over 6‐years experience in GLP and regulated field trials.

Site Information: Family owned farm, isolation for biotech work, multiple sites >20 miles apart to conduct R&D trials

Specialized Areas of Research: Conv. & GM Seeds/Traits, Yield, GLP Residue Studies, Bioefficacy, Terrestrial Ecotox, Plant stress response screening

Trial Types:

Bioefficacy ‐ Insecticide, herbicide, fungicide, seed treatments, biologicals GLP ‐ Processed Food/Feed, Magnitude of Residue, Field Accumulation in Rotational Crops, Terrestrial/Pollinator Plant growth regulator evaluations Biostimulants and Fertilizers Seeds/Traits ‐ Nutrient Composition Analysis, Expressed Trait Protein Analysis, Agronomic Characterization

Main crops at this location: corn, soybean, wheat and vegetable crops Diseases: Pests: Weeds:

Gray Leaf Spot (Corn), Common Rust (Corn), Southern Rust (Corn), Stalk Rot (Corn) Phytophthora spp. (Soy) Corn: army worms, root worm; Soy: bean leaf beetles, aphids, stinkbugs lambsquarters, ragweed spp., morning glory spp., Marestail, resistant waterhemp, resistant cocklebur,

Soil Type:

Silt Loams, 2‐3% OM

28.733.6

46.1

60.771.3

80.6 84 82.475.6

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46.2

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9.8 14.4

25.2

36.747.9

58.3 62.5 59.8

50.538.5

26.9

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0.86 1.02 2.31 3.55 4.81 5.23 4.84 4.78 3.28 2.57 2.281.27

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Annual Weather Averages for Effingham, IL




Missouri Field Station – EPA Crop Region 5 SynTech Research Inc.

112387 NW Country Rd 15002 Drexel, MO

Site Lead: Houston Howerton – Cell: 785‐331‐6971 [email protected]

Houston Howerton ‐ Over 5 years experience in GLP and field research.

Specialized Areas of Research: Conv. & GM Seeds/Traits, Yield trials, GLP Crop Residue Studies, Bioefficacy, Terrestrial Ecotox, Plant Growth Regulators

Trial Types:

Bioefficacy ‐ insecticide, herbicide, fungicide, seed treatments, biologicals GLP ‐ Processed Food/Feed, Magnitude of Residue, Field Accumulation in Rotational Crops, Terrestial/Pollinator, Non‐target plans Biostimulants and Fertilizer Plant growth regulator evaluations Seeds/Traits ‐ Nutrient Composition Analysis, Expressed Trait Protein Analysis, Agronomic Characterization


corn, soybean, wheat, alfalfa, sorghum, sunflower, solanaceae, cucurbits, root crops, brassicas, leafy greens


Pests: aphids, Japanese Beetle, corn earworm, corn rootworm, Army worm, cutworm, corn borer, thrips, tarnished plant bug, grasshoppers, bean leaf beetle, stem borer, stink bugs, soybean cyst nematode, spider mites, wireworm, cucumber beetle, squash bug, vine borer, hornworm, cabbage looper, alfalfa weevil Diseases: Fusarium, Leaf Blight, Rust, SDS, Root Rot, Blight, Brown Stem Rot, Anthracnose, SMV, Mildew, Stem Rot, Viruses Weeds: various weeds of the Midwest

Soil Type:

Clay loam, 4.5% OM

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Annual Weather Averages for Stilwell, KS

Average High Temp (°F) Average Low Temp (°F) Average Rainfall


Texas Field Station – EPA Crop Region 8 SynTech Research, Inc.

302 Arthur St Whiteface, TX 79379

Site Lead: Milo Lewis – Cell: 806‐252‐6141 [email protected]

Milo Lewis – Over 7 years of experience in contract research. The SynTech Research TX field site was opened in 2018.

Specialized Areas of Research: Conv. & GM Seeds/Traits, Yield trials, GLP Crop Residue Studies, Bioefficacy, Terrestrial Ecotox, Plant Growth Regulators, Harvest Aids

Trial Types:

Bioefficacy ‐ insecticide, herbicide, fungicide, seed treatments, biologicals GLP ‐ Processed Food/Feed, Magnitude of Residue, Field Accumulation in Rotational Crops, Terrestial/Pollinator Biostimulants and Fertilizer Plant growth regulator evaluations Harvest Aids – desiccants and defoliants Seeds/Traits ‐ Nutrient Composition Analysis, Expressed Trait Protein Analysis, Agronomic Characterization


Cotton, sorghum, corn, peanut, potato, peppers, cucurbits, forage crops


Diseases: powdery mildew in cucurbits Pests: potato psyllid, whitefly, mite, sugarcane aphid, Lygus bug Weeds: A variety of species, including glyphosate resistant marestail and Palmer amaranth

Soil Type:

Sandy loam, 1‐3% OM

54.158.9

66.7

75.483.8

90.6 92.8 91.384.5

75.2

63.6

54.1

26.430.1

3745.7

55.964.2

67.7 66.6

58.8

47.9

35.9

27.1

0.65 0.75 1.1 1.41 2.36 3.04 1.91 1.91 2.51 1.93 0.85 0.760

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Annual Weather Averages for Lubbock, TX



Mexico Field Station SynTech Research Mexico

Torre de Londres 6935 Fracc. Las Torres Aeropuerto C.P. 80144. Culiacán, Sinaloa ‐ Mexico Site Lead: Alejandro Benitez

[email protected]

Alejandro Benitez – Specialist in horticulture. Alejandro joined SynTech Research in 2015

Specialized Areas of Research: Seeds/Traits, Yield trials, GLP Crop Residue Studies, Bioefficacy, Terrestrial Ecotox, Plant Growth Regulators; Sample homogenization, storage, preparation and shipping

Trial Types:

Bioefficacy ‐ insecticide, herbicide, fungicide, seed treatments, biologicals, fertilizers, biostimulants, and plant growth regulators GLP ‐ processed food/feed, magnitude of residue, field accumulation in rotational crops, terrestrial/pollinator Seeds/Traits ‐ seed production for conventional row and vegetable crops


tomato, cucumber, peppers, radish, potato, onion, spinach, mustard green, pea, bean, soybean, eggplant, cucumber, squashes, sunflower, corn, sorghum, alfalfa, wheat, carrot, cilantro, radish, lettuce, avocado, mango, orange, papaya, strawberry


Diseases: Fusarium spp., Alternaria spp., bacterial canker, bacterial leaf spot, late blight, gray leaf spot, syringae leaf spot, black dot, downy mildew, tobacco mosaic virus, powdery mildew, leaf blight, gray mold, poinsettia mosaic virus Pests: Whitefly spp., armyworm, thrips spp., red spider mite, American serpentine leaf miner, aphid spp., pickleworm, cutworm, pepper weevil, fall armyworm, corn earworm Weeds: Palmer Amaranth, castorbean, wild sunflower, sedge spp., Johnsongrass,

Soil Type:

Clay, 1%OM

81.2 83.7 8791.7 95.5 97.5 95.2 94.1 93.6 92.8

87.982.3

50.5 50.8 52.556.5

62.271.2 73.6 73.2 72.5

66.857.6

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1.1 0.5 0.3 0.1 0.2 1.67.2 7.7 5.7 2.2 1 1.2

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www.weatherbase.com – Source for precipitation and weather averages

syntech resea · effect studies • oecd 75 semi‐field ... trial types: bioefficacy in...

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