survey of prokaryotes.pptx

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    Copyright 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings

    Lecture prepared by Mindy Miller-Kittrell, University of Tennessee, Knoxville

    M I C R O B I O L O G YWITH DISEASES BY BODY SYSTEM SECOND EDITIONChapter 11 Characterizing and Classifying Prokaryotes

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    Copyright 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings

    General Characteristics of Prokaryotic Organisms

    Prokaryotes

    Most diverse group of organisms

    Habitats

    From Antarctic glaciers to thermal hot springs

    From colons of animals to cytoplasm of other prokaryotes

    From distilled water to supersaturated brine

    From disinfectant solutions to basalt rocks

    Only a few capable of colonizing humans and causing disease

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    Copyright 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings

    General Characteristics of Prokaryotic Organisms

    [INSERT FIGURE 11.1]

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    Copyright 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings

    General Characteristics of Prokaryotic Organisms

    Reproduction of Prokaryotic Cells

    All reproduce asexually

    Three main methods

    Binary fission (most common)

    Snapping division

    Budding

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    Copyright 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings

    General Characteristics of Prokaryotic Organisms

    [INSERT FIGURE 11.2]

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    Copyright 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings

    General Characteristics of Prokaryotic Organisms

    [INSERT FIGURE 11.5]

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    7/25Copyright 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings

    General Characteristics of Prokaryotic Organisms

    Arrangement of Prokaryotic Cells

    Result from two aspects of division during binary fission

    Planes in which cells divide

    Separation of daughter cells

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    General Characteristics of Prokaryotic Organisms

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    9/25Copyright 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings

    General Characteristics of Prokaryotic Organisms

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    10/25Copyright 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings

    General Characteristics of Prokaryotic Organisms

    Endospores

    Produced by Gram-positive Bacillus and Clostridium

    Each vegetative cell transforms into one endospore

    Each endospore germinates to form one vegetative cell

    Constitute a defensive strategy against hostile or unfavorableconditions

    Serious concern to food processors, health care professionals,and governments

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    General Characteristics of Prokaryotic Organisms

    [INSERT FIGURE 11.8]

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    Modern Prokaryotic Classification

    Currently based on genetic relatedness of rRNA sequences

    Three domainsArchaea

    Bacteria

    Eukarya

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    Modern Prokaryotic Classification

    [INSERT FIGURE 11.9]

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    Survey of Archaea

    Common features

    Lack peptidoglycan Cell membrane lipids have branched hydrocarbon chains

    Classified in three phyla: Crenarchaeota, Euryarchaeota, Korarchaeota

    Reproduce by binary fission, budding, or fragmentation

    Most are cocci, bacilli, or spiral forms; pleomorphic forms exist

    Not known to cause disease

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    Survey of Archaea

    Extremophiles

    Require extreme conditions of temperature, pH, and/or salinity tosurvive

    Prominent members are thermophiles and halophiles

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    16/25Copyright 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings

    Survey of Archaea

    Extremophiles

    Thermophiles

    DNA, RNA, cytoplasmic membranes, and proteins do not

    function properly below 45C

    Hyperthermophiles

    require temperatures over 80C

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    Survey of Archaea

    [INSERT FIGURE 11.11]

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    Copyright 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings

    Survey of Archaea

    Extremophiles

    Halophiles

    Inhabit extremely saline habitats

    Depend on greater than 9% NaCl to maintain integrity of cell

    wallsMany contain red or orange pigments; require protection from

    visible and UV light

    Most studied Halobacterium salinarium

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    Copyright 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings

    Survey of Archaea

    [INSERT FIGURE 11.12]

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    Copyright 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings

    Survey of Archaea

    Methanogens

    Largest group of archaea

    Convert carbon dioxide, hydrogen gas, and organic acids tomethane gas

    Convert organic wastes in pond, lake, and ocean sediments to

    methane Some live in colons of animals; one of primary sources of

    environmental methane

    Have produced ~10 trillion tons of methane that is buried in mud

    on ocean floor

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    Copyright 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings

    Survey of Bacteria

    Deeply Branching and Phototrophic Bacteria

    Deeply branching bacteria

    Scientists believe these organisms are similar to earliest bacteria

    Autotrophic

    Live in habitats similar to those scientists think existed on earlyEarth

    Aquifex considered to represent earliest branch of bacteria

    Deinococcus has outer membrane similar to Gram-negatives,but stains Gram-positive

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    Copyright 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings

    Survey of Bacteria

    Deeply Branching and Phototrophic Bacteria

    Phototrophic bacteriaPhototrophs that contain photosynthetic lamellae; autotrophic

    Divided into five groups based on pigments and source ofelectrons for photosynthesis

    Blue-green bacteria (cyanobacteria)

    Green sulfur bacteria

    Green nonsulfur bacteria

    Purple sulfur bacteria

    Purple nonsulfur bacteria

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    Copyright 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings

    Survey of Bacteria

    Low G+C Gram-Positive Bacteria

    Mycoplasmas

    Facultative or obligate anaerobes

    Lack cell walls

    Smallest free-living cells

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    Copyright 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings

    Survey of Bacteria

    Gram-Negative Proteobacteria

    Alphaproteobacteria

    Nitrogen fixers

    Azospirillum

    Rhizobium

    Nitrifying bacteria

    Nitrobacter

    Purple nonsulfur phototrophs

    S f B i

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    C i h 2009 d i bli hi j i C i

    Survey of Bacteria